Article
Medicine, Research & Experimental
Shravan Sreenivasan, Anurag S. Rathore
Summary: This study investigated the aggregation of mAb in the presence of Fe2+ and H2O2 in saline and in vitro models. The results showed that the combined presence of Fe2+ and H2O2 increased mAb aggregation and altered protein structure and hydrophobicity. Increasing the time, temperature, and Fe2+ and H2O2 concentration further enhanced mAb aggregation. Additionally, mAb samples with copper and cobalt chlorides also resulted in degradation.
MOLECULAR PHARMACEUTICS
(2023)
Article
Biochemistry & Molecular Biology
Yukako Senga, Motomichi Doi, Masayoshi Onitsuka, Shinya Honda
Summary: This study establishes an approach to detect intracellular aggregates using a fluorescent-labeled artificial protein, AF.2A1, and finds that the relative amount of intracellular aggregates is correlated with the type of additives used during cell culture. Additionally, there is a moderate correlation between the relative amount of intracellular aggregates and that of extracellular aggregates, suggesting the secretion of intracellular aggregates.
CELL CHEMICAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Masaaki Takeuchi, Akina Esaki, Yukie Higa, Akemi Nakazono
Summary: The study found that spike IgG levels at 3 months after vaccination with BNT162b2 can reliably predict subsequent spike IgG levels up to 8 months, providing useful information for timing vaccination boosters.
Article
Immunology
Vibha Kumra Ahnlide, Therese de Neergaard, Martin Sundwall, Tobias Ambjornsson, Pontus Nordenfelt
Summary: Bacteria can interfere with antibody binding to their surfaces, posing a challenge in predicting the immunological function of bacteria-associated antibodies. The M and M-like proteins of group A streptococci have been found to reverse IgG binding orientation based on the host environment. A biophysical model has been developed to study these protein-antibody interactions and could potentially aid in the development of antibody treatments. Simulations have shown that altering IgG binding to GAS in serum can have physiological effects, providing a tool for predicting the impact of antibody treatments in the presence of bacteria with IgG-modulating surface proteins.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Sarah Esther Chang, Allan Feng, Wenzhao Meng, Sokratis A. Apostolidis, Elisabeth Mack, Maja Artandi, Linda Barman, Kate Bennett, Saborni Chakraborty, Iris Chang, Peggie Cheung, Sharon Chinthrajah, Shaurya Dhingra, Evan Do, Amanda Finck, Andrew Gaano, Reinhard Gessner, Heather M. Giannini, Joyce Gonzalez, Sarah Greib, Margrit Guendisch, Alex Ren Hsu, Alex Kuo, Monali Manohar, Rong Mao, Indira Neeli, Andreas Neubauer, Oluwatosin Oniyide, Abigail Elizabeth Powell, Rajan Puri, Harald Renz, Jeffrey M. Schapiro, Payton A. Weidenbacher, Rich Wittman, Neera Ahuja, Ho-Ryun Chung, Pras Jagannathan, Judith James, Peter S. Kim, Nuala J. Meyer, Kari Nadeau, Marko Radic, William H. Robinson, Upinder Singh, Taia T. Wang, E. John Wherry, Chrysanthi Skevaki, Eline T. Luning Prak, Pj Utz
Summary: In hospitalized COVID-19 patients, approximately 50% have detectable autoantibodies primarily targeting autoantigens associated with rare disorders. Additionally, a subset of autoantibodies develop de novo following SARS-CoV-2 infection.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Vincenza Leone, Christa Meisinger, Selin Temizel, Elisabeth Kling, Michael Gerstlauer, Michael C. Fruehwald, Katrin Burkhardt
Summary: This study aimed to evaluate the prevalence of COVID-19 infection in German children. It found a relatively low seroprevalence in children, despite under-reporting of cases. Measurement of IgG antibodies in capillary blood appeared to be an effective tool for detecting asymptomatic infections in children. However, no asymptomatic active infections were detected during the 4-month study period in the whole cohort.
Article
Multidisciplinary Sciences
Shravan Sreenivasan, Deepak Sonawat, Shyamapada Mandal, Kedar Khare, Anurag S. Rathore
Summary: The analysis of therapeutic IgG aggregates in serum using a novel fluorescence microscopy-based image processing approach offers an effective method for removing noise and retaining analyte sizes. This proposed algorithm simplifies implementation and complements existing approaches for analyzing aggregates in serum.
SCIENTIFIC REPORTS
(2021)
Review
Clinical Neurology
Christopher Nelke, Marianna Spatola, Christina B. Schroeter, Heinz Wiendl, Jan D. Lunemann
Summary: Autoantibodies have been recognized for their role in neurological diseases, and FcRn-targeted therapies show potential in treating these diseases by degrading IgG.
Article
Immunology
Biliana Todorova, Ophelie Godon, Eva Conde, Caitlin M. Gillis, Bruno Iannascoli, Odile Richard-Le Goff, Daniel Fiole, Lubka T. Roumenina, Jeanette H. W. Leusen, Andrew J. Murphy, Lynn E. Macdonald, Laurent L. Reber, Friederike Joensson, Pierre Bruhns
Summary: In mouse models of active systemic anaphylaxis, IgG-allergen immune complexes predominantly accumulate in the lungs. The accumulation of allergens in the lungs does not rely on IgG receptors, complement component C1q, neutrophils, or macrophages. Neutrophils and monocytes recruited to the lungs capture high amounts of allergens, leading to downregulation of surface IgG receptors and activation.
JOURNAL OF IMMUNOLOGY
(2022)
Article
Immunology
Yuxiao Wang, Ying Qiao, Yuqi Huo, Li Wang, Shijie Liang, Maohe Yu, Xinquan Lan, Moxin Song, Xiangjun Zhang, Ying Yan, Junjie Xu
Summary: This study aims to assess the immunogenicity and safety of the two-dose Sinovac CoronaVac vaccine for 6 months postvaccination in people living with HIV (PLWH) in China. The study found that CoronaVac was generally safe and immunogenic in PLWH, but the immune response was inferior and the antibodies vanished faster compared to HIV-negative individuals. The study suggests a shorter interval between prime-boost vaccinations for PLWH to ensure better protection.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Chemistry, Analytical
Anastasiia Misiura, Hao Shen, Lawrence Tauzin, Chayan Dutta, Logan D. C. Bishop, Nicole C. Carrejo, Jorge O. Zepeda, Shahryar Ramezani, Nicholas A. Moringo, Amanda B. Marciel, Peter J. Rossky, Christy F. Landes
Summary: This study investigated the conformational changes of antibodies during ion-exchange chromatography, revealing that absence of salt enhances electrostatic attraction, promotes surface-induced unfolding, slows antibody motion, and decreases elution rate. These findings provide new insights into the influence of antibody structural changes on macroscopic elution profiles.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Thuy Tran, Erik Martinsson, Sergio Vargas, Ingemar Lundstrom, Carl-Fredrik Mandenius, Daniel Aili
Summary: The production of therapeutic monoclonal antibodies is a complex process that requires extensive analytical characterization. Aggregation of antibodies is common and problematic, as it can affect the product quality and immunogenicity. Rapid detection of aggregates is challenging with current analytical techniques.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Michele Di Foggia, Carla Boga, Gabriele Micheletti, Benedetta Nocentini, Paola Taddei
Summary: Many restoring formulations for damaged hair keratin have been developed, claiming repair occurs through the reconstruction of disulfide bridges of keratin. Experimental results showed that shikimic acid and maleate-based restoring agents interacted with hair fibers, modifying the hair structure.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biotechnology & Applied Microbiology
Jian Ju, Xinyu Zhang, Lin Li, Sagar Regmi, Guoqiang Yang, Shixing Tang
Summary: A novel fluorescent lateral flow immunoassay (LFA) platform was developed for the rapid and accurate detection of SARS-CoV-2-specific antibodies. The platform employs aggregation-induced emission carbon dots conjugated with the SARS-CoV-2 spike protein, providing high sensitivity and potential applications in public health and clinical research.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Physical
Yanli Li, Xunhao Wang, Jie Zhang, Zhichao Wu
Summary: Silver nanoparticles (AgNPs) are widely studied and used due to their antimicrobial and optical properties. When AgNPs encounter physiological environments, they form a protein corona (PC) due to rapid encapsulation by proteins. The type, concentration, and ratio of proteins in the PC were altered during this process. Dynamic light scattering (DLS) and multi-barreled light scattering (Turbiscan) were used to study the physicochemical reactions. The results showed that IgG preferentially adsorbed and increased AgNPs aggregation, while Fib adsorbed and decreased aggregation. Fib had a greater inhibitory effect than IgG when both proteins were present. At high ionic strength, IgG and Fib cooperated to inhibit AgNPs aggregation, with Fib having a greater effect. The presence of a higher amount of protein led to a more vigorous movement of protein molecules around AgNPs corona.
COLLOID AND INTERFACE SCIENCE COMMUNICATIONS
(2023)