Article
Chemistry, Physical
Qinyu Li, Xiaojia Chen, Xiangcen Liu, Zheng Chen, Yang Han, Peng Zhou, Jiping Shi, Zhijun Zhao
Summary: The esterase PTCL1-EstA from Paenarthrobacter aurescens TC1 was successfully expressed and purified. Homology modeling and substrate docking predicted the active site residues in PTCL1-EstA and its enzymatic activity and stability under different conditions were analyzed. Fermentation medium optimization increased the enzyme activity of PTCL1-EstA, showcasing its potential for industrial production.
