Article
Fisheries
Vitor Hugo Penariol Morante, Carlos Eduardo Copatti, Antonio Ramires Lyra Souza, Mateus Matiuzzi da Costa, Luis Gustavo Tavares Braga, Anderson Miranda Souza, Fulvio Viegas Santos Teixeira de Melo, Antonio Cleber da Silva Camargo, Jose Fernando Bibiano Melo
Summary: The study found that using 80 g per kg of crude grape extracts (CGEs) as a feed additive had a positive impact on the growth performance and immunity of tambaqui juveniles without affecting metabolic and hematologic parameters.
Article
Chemistry, Physical
Lan Tang, Jie Hu, Sen Mei, Di Wu, Jing Zhang, Wen Wu, Hanmei Li, Hui Li
Summary: The study found that the interaction between 4,4'-bis(maleimido)azobenzene and human serum albumin/lysozyme is mainly through static quenching, driven by hydrogen bonding and van der Waals forces, with lysozyme being more temperature sensitive. The binding of azobenzene within the proteins leads to significant changes in their secondary structures and the hydrophobic environment around active sites, impacting the transportation and distribution of the compound.
JOURNAL OF MOLECULAR STRUCTURE
(2022)
Article
Biochemistry & Molecular Biology
Takahiko Matsushita, Hinako Yamochi, Shinzo Omiya, Tetsuo Koyama, Ken Hatano, Koji Matsuoka
Summary: Polyacrylamides incorporating serine, aspartic acid, and histidine, the amino acids in the catalytic triad of natural serine protease chymotrypsin, were synthesized and their protein cleavage activity was examined. SDS-PAGE analysis revealed that some of the synthesized ternary copolymers were able to cleave bovine and human serum albumins. Homopolymers incorporating a single type of amino acid also showed protein cleavage abilities. These findings suggest that polymers functionalized with amino acids have potential as proteolytic artificial enzymes.
BIOORGANIC & MEDICINAL CHEMISTRY
(2023)
Article
Chemistry, Physical
Jinjun Tian, Zhenghai Shi, Gongke Wang
Summary: In this study, the binding interaction between silver nanoparticles and human serum albumin was investigated. The results showed that the interaction is driven by entropy, leading to conformational changes in the protein and a decrease in alpha-helix content. Additionally, the binding followed pseudo-second-order kinetics and fit the Freundlich model for multilayer adsorption.
Article
Biochemistry & Molecular Biology
Jimin Park, Mi-Sun Kim, Taeseong Park, Young Hwan Kim, Dong Hae Shin
Summary: Pharmaceutical-grade HSA has altered oxidative and ligand-binding properties compared to native HSA, which provides important insights for the production of p-HSA solutions and HSA-based biomaterials with higher biorelevance.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Romana Parveen, Zeba Tarannum, Sher Ali, Sadaf Fatima
Summary: Protein aggregation is the major cause of several acute amyloid diseases, and in this study, nanoclays were used to inhibit the aggregation of model proteins HSA and HL. The results showed that all nanoclays inhibited the DTF-induced aggregation, with bentonite and MMT K-10 being particularly effective in slowing down the nucleation stage, as observed through various biophysical techniques. This suggests that nanoclays could be used as artificial chaperones for effective treatment against protein aggregation-related disorders.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemical Research Methods
Aleks Shin, Yahor Vazmitsel, Shawn Connolly, Kuanysh Kabytaev
Summary: Lysine residues of proteins react with glucose to form Amadori products, and this reaction becomes more prevalent in hyperglycemic conditions, leading to severe medical complications. The structure and conformation of proteins affect the reactivity of lysine sites, and our study aimed to identify these sites in albumin and measure their glycation affinities.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Meiyun Shi, Xujian Duan, Xinyue Zheng, Di Liu, Yuncheng Ge, Ning Zhang, Yajun Liu, Hongyu Xue, Lei Yin
Summary: The quantification of albumin is important in clinical medicine. A highly selective and robust assay combining chymotrypsin/trypsin digestion and targeted LC-MS/MS technique was developed to determine human serum albumin (HSA) in human plasma. The assay showed good accuracy and precision in determining HSA concentrations.
ANALYTICAL BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Sadaf Khan, Aabgeena Naeem
Summary: This study examines the capability of bovine serum albumin (BSA) to function as an extracellular chaperone on human hemoglobin (Hb) at pH 7.4. The optimal temperature of 55 degrees C was determined for studying Hb amyloid formation. BSA demonstrated chaperone-like activity on Hb at 55 degrees C with a client protein:BSA ratio of 1:10, indicating the effects of macromolecular crowding. BSA was found to inhibit Hb dissociation, protein aggregation, and cause fibril depolymerization.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Chemistry, Applied
Mariane Gonsalves Santos, Diailison Teixeira de Carvalho, Lucas Belga Caminitti, Bruna Bueno Alves de Lima, Marcello Henrique da Silva Cavalcanti, Daniel Felipe Rocha dos Santos, Luciano Sindra Virtuoso, Daniela Battaglia Hirata, Eduardo Costa Figueiredo
Summary: Fe3O4@TEOS@BSA nanoparticles modified with tetraethyl orthosilicate and bovine serum albumin are efficient in separating lysozyme from egg white at different pH conditions. The extracted lysozyme maintains high enzymatic activity. These particles are easily recyclable and can be reused multiple times.
Article
Biochemistry & Molecular Biology
Francis H. C. Tsao, Keith C. Meyer
Summary: The age-dependent conformational stability of human serum albumin was evaluated using the fluorescent bilayer liposome assay. It was found that albumin in the sera of older individuals exhibited hydrophobic effects on liposomes and made liposomal membrane more prone to hydrolysis, while albumin in the sera of younger individuals remained stable. These findings suggest that age-related changes in albumin stability may impact its normal functions and membrane integrity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Attila Forgacs, Madalina Ranga, Istvan Fabian, Jozsef Kalmar
Summary: Mesoporous silica aerogels have potential applications in various fields, and understanding their interactions with biomolecules is crucial for optimal performance. This study investigated the interactions between bovine serum albumin (BSA) and silica aerogels, revealing a multi-step binding mechanism and the influence of pH on their interaction strength.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Sarah McColman, Rui Li, Selena Osman, Amanda Bishop, Kathleen P. Wilkie, David T. Cramb
Summary: This study used Fluorescence Correlation/Cross Correlation Spectroscopy and Fluorescence Resonance Energy Transfer to directly analyze four different nanoparticle systems, finding surface heterogeneity to be a key factor in protein binding to these nanoparticles. The early hard corona is conceptualized as low-ratio, non-uniform binding rather than a uniform monolayer.
Article
Biochemistry & Molecular Biology
Eszter Fliszar-Nyul, Zelma Faisal, Renata Skaper, Beata Lemli, Bayarsaikhan Bayartsetseg, Csaba Hetenyi, Patrik Gombos, Andras Szabo, Miklos Poor
Summary: Beauvericin, cyclopiazonic acid, and sterigmatocystin are emerging mycotoxins that can contaminate food and animal feed, resulting in economic losses and health risks. Human serum albumin can form stable complexes with these mycotoxins, influencing their toxicokinetics and potentially serving as a cost-effective affinity protein. This study investigated the interactions of these mycotoxins with human serum albumin using various techniques, revealing the formation of low to moderately strong complexes. The interactions of cyclopiazonic acid with site markers suggest potential toxicological importance.
Article
Biochemistry & Molecular Biology
Alexey Chubarov, Anna Spitsyna, Olesya Krumkacheva, Dmitry Mitin, Daniil Suvorov, Victor Tormyshev, Matvey Fedin, Michael K. Bowman, Elena Bagryanskaya
Summary: The Pulsed Dipolar Spectroscopy (PDS) methods of Electron Paramagnetic Resonance (EPR) were used to study reversible non-covalent dimers of Human Serum Albumin (HSA), showing that chemical modifications of Cys-34 can affect dimerization. The weak non-covalent dimers formed by HSA at physiological concentrations have a well-defined structure and can easily revert back to monomers, playing an important role in transport and binding processes.
Article
Chemistry, Multidisciplinary
Margarita E. Aliaga, Luis Garcia-Rio, Marcia Pessego, Rodrigo Montecinos, Denis Fuentealba, Ivan Uribe, Manuel Martin-Pastor, Olimpo Garcia-Beltran
NEW JOURNAL OF CHEMISTRY
(2015)
Article
Chemistry, Multidisciplinary
Daiane G. Liz, Alex M. Manfredi, Michelle Medeiros, Rodrigo Montecinos, Borja Gomez-Gonzalez, Luis Garcia-Rio, Faruk Nome
CHEMICAL COMMUNICATIONS
(2016)
Article
Biochemistry & Molecular Biology
Gonzalo Vera, Carlos F. Lagos, Sebastian Almendras, Dan Hebel, Francisco Flores, Gissella Valle-Corvalan, C. David Pessoa-Mahana, Jaime Mella-Raipan, Rodrigo Montecinos, Gonzalo Recabarren-Gajardo
Article
Polymer Science
Fabian Avila-Salos, Alfredo Pereira, Moises A. Rojas, Mario Saavedra-Torres, Rodrigo Montecinos, Sebastian Bonardd, Caterina Quezada, Soledad Saldias, David Diaz Diaz, Angel Leivaa, Deodato Radic, Cesar Saldias
EUROPEAN POLYMER JOURNAL
(2017)
Article
Chemistry, Physical
E. Ortega, R. Montecinos, L. Cattin, F. R. Diaz, M. A. del Valle, J. C. Bernede
JOURNAL OF MOLECULAR STRUCTURE
(2017)
Article
Chemistry, Multidisciplinary
Rodrigo Montecinos, Margarita E. Aliaga, Paulina Pavez, Jose G. Santos
NEW JOURNAL OF CHEMISTRY
(2017)
Article
Chemistry, Organic
Borja Gomez-Gonzalez, Vitor Francisco, Rodrigo Montecinos, Luis Garcia-Rio
ORGANIC & BIOMOLECULAR CHEMISTRY
(2017)
Article
Chemistry, Physical
Eduardo H. Wanderlind, Daiane G. Liz, Adriana P. Gerola, Ricardo F. Affeldt, Vanessa Nascimento, Lizandra C. Bretanha, Rodrigo Montecinos, Luis Garcia-Rio, Haidi D. Fiedler, Faruk Nome
Article
Chemistry, Physical
V. E. Bahamonde-Padilla, Javier Espinoza, B. E. Weiss-Lopez, J. J. Lopez Cascales, R. Montecinos, R. Araya-Maturana
JOURNAL OF CHEMICAL PHYSICS
(2013)
Article
Chemistry, Organic
Rodrigo Montecinos, Fernanda Diaz-Wilson, Alexis Bravo-Sepulveda, Cristian O. Salas, Gonzalo Recabarren-Gajardo, Faruk Nome
JOURNAL OF PHYSICAL ORGANIC CHEMISTRY
(2019)
Article
Chemistry, Multidisciplinary
Rodrigo Montecinos, Stephanie Neira-Jimenez
JOURNAL OF THE CHILEAN CHEMICAL SOCIETY
(2014)
Article
Chemistry, Physical
Rajendran Satheeshkumar, Rodrigo Montecinos, Ariesny Vera, Karnam Jayarampillai Rajendra Prasad, Werner Kaminsky, Cristian O. Salas
Summary: A novel dispirocompound was synthesized through 1,3-dipolar cycloaddition reaction, with its crystal structure reported and chemical properties analyzed. DFT calculations were utilized to study molecular electrostatic potential, non-covalent interactions, Hirshfeld Surface, and nonlinear optical properties of the compound, providing insights into its chemical reactivity.
JOURNAL OF MOLECULAR STRUCTURE
(2021)
Article
Chemistry, Multidisciplinary
Rodrigo Montecinos, Margarita E. Aliaga, Paulina Pavez, Patricio Cornejo, Jose G. Santos
Summary: The study investigates the effects of different solvents, amines, and electrophilic groups on the kinetics, mechanism, and nucleofugality hierarchy of nucleophilic substitution reactions. The results show a mechanistic dependence on solvent polarity, with the relative polarization of the reactive centers and stabilization of nucleofuges being the main factors controlling product distribution.
NEW JOURNAL OF CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Eduardo Vieira Silveira, Rodrigo Montecinos, Leandro Scorsin, Luis Garcia-Rio, Michelle Medeiros, Vanessa Nascimento, Faruk Nome, Ricardo F. Affeldt, Gustavo A. Micke
Summary: The study compared the spontaneous decarboxylation reactions of different nitrated CBI derivatives and the kinetic effects of cationic pillararenes on the reaction rates, revealing unprecedented inhibitory effects in certain cases. The data elucidated that the geometries of the complexes play a crucial role in modifying the kinetic effects by affecting factors such as hydrogen bonding, bond polarization, and electronic effects. The results encourage the incorporation of spatiotemporal and preorganization theories in interpreting supramolecular and enzymatic catalysis.
NEW JOURNAL OF CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Rodrigo Montecinos, Marcela Gazitua, Jose G. Santos
NEW JOURNAL OF CHEMISTRY
(2019)