☆ 4.2 Article

Shotgun Mass Spectrometry Workflow Combining IEF and LC-MALDI-TOF/TOF

PROTEIN JOURNAL (2010)

期刊

PROTEIN JOURNAL

卷 29, 期 2, 页码 99-102

出版社

SPRINGER

DOI: 10.1007/s10930-009-9227-7

关键词

Shotgun mass spectrometry; MALDI-TOF; HPLC; IEF; Proteomics

类别

Biochemistry & Molecular Biology

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摘要

We present a high throughput shotgun mass spectrometry workflow using a bidimensional peptide fractionation procedure consisting of isoelectric focusing and RP-HPLC prior to mass spectrometric analysis, with the aim of optimizing peptide separation and protein identification. As part of the workflow we used the 'Isotope-Coded Protein Labeling' (ICPL) method for accurate relative quantitation of protein expression. Such workflow was successfully applied to a comparative proteome analysis of schizophrenia versus healthy control brain tissues and can be an alternative to proteome researches.

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