期刊
PROTEIN EXPRESSION AND PURIFICATION
卷 92, 期 1, 页码 67-76出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.pep.2013.09.001
关键词
HEK-293; CHO-DG44; Transient gene expression; Transfection; Recombinant protein; Polyethylenimine; Suspension culture; Orbital shaking; Mammalian cells
类别
资金
- Swiss National Science Foundation
- Swiss Innovation Agency CTI-KTI
- EPFL
Transient gene expression (TGE) from mammalian cells is an increasingly important tool for the rapid production of recombinant proteins for research applications in biochemistry, structural biology, and biomedicine. Here we review methods for the transfection of human embryo kidney (HEK-293) and Chinese hamster ovary (CHO) cells in suspension culture using the cationic polymer polyethylenimine (PEI) for gene delivery. (C) 2013 Elsevier Inc. All rights reserved.
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