Article
Biochemical Research Methods
Jui-Chung Hong, Hao-Chun Fan, Po-Jiun Yang, Da-Wei Lin, Hsuan-Chen Wu, Hsiao-Chun Huang
Summary: The study shows that localized proteolysis can be employed to construct intracellular asymmetry in Escherichia coli, providing a new strategy for polarization in developmental cell biology. Circuits designed in this study may help to expand the synthetic biology repository for the engineering of synthetic morphogenesis.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Biology
Shengchen Wang, Faying Zhang, Meng Mei, Ting Wang, Yueli Yun, Shihui Yang, Guimin Zhang, Li Yi
Summary: The study developed a fluorescence-assisted single-cell methodology (SPEC) to explore protein-protein interactions in E. coli cells, which offers high sensitivity, easy manipulation, and broad temperature adaptability.
COMMUNICATIONS BIOLOGY
(2021)
Article
Biochemical Research Methods
Xianan Zhang, Nico Betterle, Diego Hidalgo Martinez, Anastasios Melis
Summary: This study investigates the stability of recombinant proteins in cyanobacteria and finds that heterologous proteins are more stable when in fusion constructs compared to when they are present alone. The results set a foundation for overexpression of pathway enzymes in synthetic biology approaches.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Maricarmen Marti, Fernando Merwaiss, Anamarija Butkovic, Jose-Antonio Daros
Summary: This study reports the production of genetically encoded plant-derived viral nanoparticles (VNPs) decorated with nanobodies. This system may provide an attractive alternative for sustainable production of nanobody-containing nanomaterials for diagnostic and therapeutic purposes in plants.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Immunology
Ming-Hao Yang, Chung-Chi Hu, Chi-Hzeng Wong, Jian-Jong Liang, Hui-Ying Ko, Meng-Hsun He, Yi-Ling Lin, Na-Sheng Lin, Yau-Heiu Hsu
Summary: A new binary epitope-presenting CVP platform has been developed based on bamboo mosaic virus using SrtA-mediated ligation technology, showing promise for vaccine candidate development and mass production.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Virology
John F. Murphy, H. Tucker Hallmark, Thiruvarangan Ramaraj, Anitha Sundararajan, Faye Schilkey, Aaron M. Rashotte
Summary: Through transcriptomic RNA sequencing, distinct profiles of Differentially Expressed Genes (DEGs) were identified for each TEV strain in infected pepper plants. The number of DEGs increased with the severity of disease symptoms, and the DEG patterns from 7 to 14 days post-inoculation corresponded to the severity of disease. Surprisingly, there were very few overlapping DEGs among the TEV strains, with no overlapping DEGs between all three strains at 7 or 14 days post-inoculation.
Article
Biotechnology & Applied Microbiology
Fernando Merwaiss, Enrique Lozano-Sanchez, Joao Zulaica, Luciana Rusu, Marta Vazquez-Vilar, Diego Orzaez, Guillermo Rodrigo, Ron Geller, Jose-Antonio Daros
Summary: Researchers have developed genetically encoded multivalent viral nanoparticles that can recognize and neutralize SARS-CoV-2 Spike protein. These nanoparticles exhibited higher neutralization activity compared to the corresponding nanobodies in a dimeric format. The ability to produce VNP cocktails in plants makes them an attractive alternative to standard antibodies for multiple applications in diagnosis and therapeutics.
PLANT BIOTECHNOLOGY JOURNAL
(2023)
Article
Biotechnology & Applied Microbiology
Shiyu Chen, Scott Lovell, Sumin Lee, Matthias Fellner, Peter D. Mace, Matthew Bogyo
Summary: A phage display method was developed to screen for covalent inhibitors that bind to protein targets by introducing a reactive linker to form cyclic peptides and an electrophilic 'warhead' to react with target nucleophiles. This approach identified cyclic peptides that irreversibly inhibited cysteine protease and serine hydrolase with high specificity and potency. It enables rapid, unbiased screening to identify new classes of highly selective covalent inhibitors for diverse molecular targets.
NATURE BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Mingguang Cui, Seunghwan Lee, Sung Hwan Ban, Jae Ryun Ryu, Meiying Shen, Soo Hyun Yang, Jin Young Kim, Seul Ki Choi, Jaemin Han, Yoonhee Kim, Kihoon Han, Donghun Lee, Woong Sun, Hyung-Bae Kwon, Dongmin Lee
Summary: Proteases, particularly potyviral protease, have been employed as molecular switches in synthetic biological circuits. LAUNCHER, a single-component switch utilizing circularly permutated tobacco etch virus protease and blue-light-gated substrate, offers superior signal-to-noise ratio and precise payload release, making it suitable for diverse cellular applications and enhanced circuit performance.
NATURE CHEMICAL BIOLOGY
(2023)
Article
Horticulture
Zhenghai Mo, Yu Chen, Min Zhai, Kaikai Zhu, Jiping Xuan, Longjiao Hu
Summary: A virus-induced gene silencing (VIGS) system based on tobacco rattle virus (TRV) was constructed and optimized for functional genomics studies in pecan. The optimized conditions included the use of a TRV-based vector with a 300 bp target sequence, syringe infiltration method, inoculation of 'Pawnee' or 'Shaoxing' seedlings, and exposure to a temperature cycle of 24 degrees C (day, 12 h)/16 degrees C (night, 12 h). The established VIGS system was successfully used for reverse genetic studies in pecan.
SCIENTIA HORTICULTURAE
(2023)
Article
Chemistry, Multidisciplinary
Sea On Lee, Qi Xie, Stephen D. Fried
Summary: By rearranging the topology of a group I self-splicing intron from T4 bacteriophage, researchers successfully circularized mRNA to optimize protein expression. Through a modified PCR method generating mutants with higher expression levels, they demonstrated the potential of loopable translation technology for creating large peptide chains.
ACS CENTRAL SCIENCE
(2021)
Article
Chemistry, Applied
Ying Li, Zhong Zhang, Alireza Abbaspourrad
Summary: The use of phycocyanin in acidic food products is limited due to its susceptibility to aggregation. However, by modifying the protein, its stability and functionality can be improved for applications in acidic environments.
FOOD HYDROCOLLOIDS
(2022)
Article
Chemistry, Medicinal
Diego Garcia Jimenez, Vasanthanathan Poongavanam, Jan Kihlberg
Summary: We have analyzed the use of macrocycles in drug discovery by studying FDA-approved drugs, clinical candidates, and recent literature. Current drugs are mainly used for infectious diseases and oncology, while oncology is also the major indication for clinical candidates and in the literature. Most macrocyclic drugs bind to targets with challenging binding sites. Natural products provide the majority of drugs and clinical candidates, but macrocycles in ChEMBL have simpler structures. Although macrocycles usually fall beyond the Rule of 5 chemical space, 30-40% of drugs and clinical candidates are orally bioavailable. Simple bi-descriptor models can be used in design to distinguish oral drugs from parenterals. We expect that advances in conformational analysis and inspiration from natural products will enhance the de novo design of macrocycles.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Jing Li, Si Chen, Run-Shuang Qiu, Li-Zhen Zhang, Yue Chen, Xue Zheng, Ting-Ting Li, Li-Hua Zhao, Zhong-Kai Zhang
Summary: This study found that the NSs protein of Tomato zonate spot virus acts as an RNA silencing suppressor in tobacco plants, inhibiting the transcription and protein expression of viral genes and alleviating the symptoms of viral infection. The study also discovered that the NSs protein suppresses the expression of the Zingipain-2-like protein, which co-localizes with NSs.
Article
Chemistry, Inorganic & Nuclear
Qianrui Zhao, Zhiwen Tang, Yuede Pan, Jingjing Han, Jinbiao Yang, Yongqiang Guo, Xiangqian Lai, Zhewei Yang, Gang Li
Summary: The solubility product constant (K-sp) is an important concept in inorganic chemistry that determines the solubility of ionic compounds. K-sp guided precipitation reactions are useful for obtaining solid materials or removing unwanted ions from solutions. The K-sp gap between two solids triggers precipitation transformation reactions, and this phenomenon can be applied to prepare transition metal sulfides from corresponding hydroxides in sodium sulfide solutions.
INORGANIC CHEMISTRY FRONTIERS
(2023)
Article
Biochemical Research Methods
Juri Sakata, Toshifumi Tatsumi, Akira Sugiyama, Akihiro Shimizu, Yuya Inagaki, Hiroto Katoh, Takefumi Yamashita, Kazuki Takahashi, Sho Aki, Yudai Kaneko, Takeshi Kawamura, Mai Miura, Masazumi Ishii, Tsuyoshi Osawa, Toshiya Tanaka, Shumpei Ishikawa, Masanobu Tsukagoshi, Michael Chansler, Tatsuhiko Kodama, Motomu Kanai, Hidetoshi Tokuyama, Kenzo Yamatsugu
Summary: This study reports an expression, refolding, and purification method for antibody-mimetic drug conjugate (AMDC). The AMDC uses a variable heavy chain of heavy chain-only antibodies (VHHs) as the targeting protein and non-covalently binds to a potent DNA-alkylating drug. The AMDC shows strong cytotoxic effects on cancer cells.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Yao Wang, Shaoting Weng, Yajie Tang, Sen Lin, Xiayue Liu, Wenhui Zhang, Gang Liu, Boomi Pandi, Yinrong Wu, Lei Ma, Lin Wang
Summary: In this study, a high yield of recombinant CD20 and claudin 18.2 proteins was achieved using an in vitro coupled transcription-translation system. The results showed that rituximab has a high affinity with CD20 protein. This study provides a novel concept for promoting the expression of multi-pass transmembrane proteins and lays the foundation for large-scale industrial production of membrane-associated drug targets.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Xiaobing Chen, Zijuan Zou, Wei Li, Xu Dong, Yi Chen, Yan Lu, Mingyue Zhu, Mengsen Li, Bo Lin
Summary: In this study, the fusion protein ImI-AFP3, composed of alpha-Conotoxin ImI and human alpha fetoprotein domain 3 (AFP3), was found to inhibit the growth and migration of lung cancer cells and showed synergistic effects with the drug gefitinib. These findings suggest that ImI-AFP3 is a promising candidate for the development of anticancer drugs.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Angel Castillo-Corujo, Mirva J. Saaranen, Lloyd W. Ruddock
Summary: This study successfully expressed two Fabs antibodies in the cytoplasm of E. coli using the CyDisCo system, achieving high yields and biological activity under industrially relevant fermentation conditions.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Junling Guo, Zhongyi Cheng, Zhemin Zhou
Summary: This study focused on a halophilic archaeal nitrile hydratase (NHase) and found that it exhibited higher tolerance to substrates and products compared to NHases from other sources. The unique genetic structure of this highly stable archaeal NHase could provide a theoretical foundation for modifying and enhancing the industrial application of NHase.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Yunlong Shen, Ruirui Zhang, Xiaohua Jiang, Jinliang Yang
Summary: The study developed a monoclonal antibody, B1M023, that can bind to LILRB1 with high affinity and block its binding to HLA-G. This antibody can promote the activation and IFN-gamma secretion of T cells, suggesting its potential applications in concomitant diagnosis and tumor immunotherapy.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Jun Jiang, Zhengqiang Jiang, Qiaojuan Yan, Susu Han, Shaoqing Yang
Summary: A novel alginate lyase gene from a marine bacterium showed efficient enzyme activity and stability, producing high conversion ratios of alginate oligosaccharides.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Xianghua Xiong, Yujin Qiu, Jiahao Zheng, Ling Zhou, Qingyang Wang, Jinglun Pang, Weicai Zhang, Huipeng Chen, Gang Liu, Xiaodong Han
Summary: A specific monoclonal antibody ML419 has been found to disrupt the recognition between Botulinum neurotoxin serotype A (BoNT/A) and FGFR3, effectively preventing BoNT/A from entering neurons. In vivo experiments show that ML419 has a strong protective effect, making it a promising candidate for the development of therapeutics against BoNT/A.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Maria Rain Jennings, Soohyon Min, Grace S. Xu, Kassandra Homayuni, Bhavana Suresh, Yusef Amir Haikal, John Blazeck
Summary: This study successfully produced Homo sapiens adenosine deaminase isoform 1 (HsADA1) and its variants through optimizing the recombinant expression process. The D8N variant of HsADA1 was found to be about 30% less active than the wildtype, but it better retained its activity in human serum. Additionally, the study revealed a previously undescribed phenomenon involving albumin that contributed to the increased activity of HsADA1 and the D8N variant in serum.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Oinam Sangita Devi, Senjam Sunil Singh, K. Rana, Sorokhaibam Jibankumar Singh, Wayenbam Sobhachandra Singh
Summary: A new lectin with hemagglutination activity was purified from the rhizome of Xanthosoma violaceum Schott. The lectin showed different reactions towards human red blood cells of different blood groups. It exhibited optimal hemagglutination activity at specific temperature and pH range, and showed good stability.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
