Review
Medicine, General & Internal
Dan Pugh, Eoin D. O'Sullivan, Fiona Ai Duthie, Philip Masson, David Kavanagh
Summary: AHUS is a rare disorder characterized by abnormal complement regulatory proteins, leading to kidney failure and death in the past. However, new therapies such as terminal complement inhibition show promising outcomes, although based on very low-quality evidence from single-arm studies. Careful consideration of future data is needed for better understanding of treatment duration and adverse outcomes.
COCHRANE DATABASE OF SYSTEMATIC REVIEWS
(2021)
Review
Pharmacology & Pharmacy
Yahiya Y. Syed
Summary: Ravulizumab is a humanized monoclonal antibody indicated for the treatment of aHUS, with a convenient maintenance dosage regimen compared to eculizumab, and has shown good efficacy and tolerability in clinical trials.
Article
Urology & Nephrology
Caroline Duineveld, Romy Bouwmeester, Joost W. van der Heijden, Stefan P. Berger, Nicole C. A. J. van de Kar, Jack F. M. Wetzels
Summary: This interim analysis evaluated the safety of re-treatment with eculizumab in 11 patients with suspected relapse. Results showed that re-treatment with eculizumab after relapse is safe. Transplanted patients responded better to eculizumab therapy compared to those with aHUS in native kidneys.
CLINICAL KIDNEY JOURNAL
(2021)
Review
Cell Biology
Sonali Nashine
Summary: This article discusses the contribution of aging to ocular diseases such as AMD, and introduces potential therapeutic candidates for AMD along with their known mechanisms of cytoprotection.
Article
Urology & Nephrology
Gema Ariceta, Fadi Fakhouri, Lisa Sartz, Benjamin Miller, Vasilis Nikolaou, David Cohen, Andrew M. Siedlecki, Gianluigi Ardissino
Summary: Discontinuation of eculizumab in patients with aHUS carries risks of TMA recurrence and renal failure. A thorough assessment of risk factors prior to discontinuing eculizumab is essential for patient management.
CLINICAL KIDNEY JOURNAL
(2021)
Article
Ophthalmology
Ling Chen, Jeffrey D. Messinger, Deepayan Kar, Jacque L. Duncan, Christine A. Curcio
Summary: The study revealed that the volume of BLinD may be associated with the progression risk of AMD, with BLinD potentially acting as a precursor to type 1 macular neovascularization. Pre-BLinD is more prevalent in control eyes, while BLinD/soft drusen is more common in AMD eyes.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2021)
Review
Biochemistry & Molecular Biology
Reem H. ElSheikh, Muhammad Z. Chauhan, Ahmed B. Sallam
Summary: Age-related macular degeneration (AMD) is a leading cause of blindness in the elderly population, with neovascular AMD (nAMD) being the main contributor to visual loss. Currently approved anti-VEGF agents have shown favorable prognosis for nAMD but come with a substantial financial burden for patients and the healthcare system due to their high cost and the need for repeated treatments and visits. Emerging therapies aim to prolong treatment intervals and introduce new modalities for better results.
Review
Biochemistry & Molecular Biology
Ruth M. Galindo-Camacho, Cristina Blanco-Llamero, Raquel da Ana, Mayra A. Fuertes, Francisco J. Senorans, Amelia M. Silva, Maria L. Garcia, Eliana B. Souto
Summary: This article reviews the challenges in the treatment of age-related macular degeneration (AMD), innovative nanoformulations, 3D bioprinting, and techniques for monitoring progress.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Transplantation
Mendy ter Avest, Romy N. Bouwmeester, Caroline Duineveld, Kioa L. Wijnsma, Elena B. Volokhina, Lambertus P. W. J. van den Heuvel, David M. Burger, Jack F. M. Wetzels, Nicole C. A. J. van de Kar, Rob ter Heine
Summary: This study evaluated the pharmacokinetics and pharmacodynamics of eculizumab in patients with atypical haemolytic uraemic syndrome (aHUS) and proposed improved dosing strategies. The study found that individualized dosing strategy could improve treatment response and reduce treatment costs by prolonging the dosing interval.
NEPHROLOGY DIALYSIS TRANSPLANTATION
(2023)
Article
Plant Sciences
Yuli Li, Xing Li, Xiaoya Li, Zhihong Zeng, Niall Strang, Xinhua Shu, Zhoujin Tan
Summary: Traditional Chinese Medicine has shown promising efficacy in the treatment of age-related macular degeneration, but there is currently a lack of comprehensive literature review on its application. Future research should focus on understanding the mechanism of action and bioactive components of TCM therapies to improve their impact.
JOURNAL OF ETHNOPHARMACOLOGY
(2022)
Article
Medicine, General & Internal
Alicia Darwin, Leonger Malpica, Jugraj Dhanoa, Hamza Hashmi
Summary: Haemolytic uraemic syndrome (HUS) encompasses typical HUS associated with Shiga toxin and atypical aHUS associated with overactivation of the alternative complement pathway. Drug-induced aHUS cases vary in presentation, emphasizing the importance of early detection and treatment.
Article
Ophthalmology
Duo Li, Ting-Ting Wei, Jiping Cai, Tian-Hua Xie, Yong Yao, Lingpeng Zhu
Summary: This research explores the role of Smurf1 in retina degeneration and finds that inhibiting Smurf1 can alleviate retina injury and inflammation activation.
EXPERIMENTAL EYE RESEARCH
(2023)
Article
Medicine, General & Internal
Thibaud Mathis, Batoul El Ameen, Mayeul Chaperon, Yasmine Serrar, Francois Devin, Mikhail Dziadzko, Amina Rezkallah, Laurent Kodjikian
Summary: The aim of this study was to evaluate whether anatomical criteria based on optical coherence tomography (OCT)-guided strategy could determine the decision to retreat in patients with neovascular age-related macular degeneration (nAMD). A cross-sectional study of 142 eyes treated for nAMD was conducted, and the OCT-guided strategy showed a high level of agreement with the gold standard for treatment decisions. The findings suggest that selected nAMD patients under anti-VEGF treatment can be monitored without regular visual acuity testing.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Urology & Nephrology
Jose Portoles, Ana Huerta, Emilia Arjona, Eva Gavela, Marisa Aguera, Carlos Jimenez, Teresa Cavero, Domingo Marrero, Santiago Rodriguez de Cordoba, Fritz Diekmann
Summary: Both pre-aHUS and de novo patients showed different clinical profiles and responses to ECU treatment. Genetic studies are important in determining risks of relapse and guiding treatment decisions. ECU may be considered as a preemptive treatment for patients at moderate or high risk of recurrence.
CLINICAL KIDNEY JOURNAL
(2021)
Article
Transplantation
Shuichi Ito, Hiroshi Hataya, Akira Ashida, Riku Hamada, Tomoaki Ishikawa, Yumiko Ishikawa, Akihiko Shimono, Takao Konomoto, Tomoki Miyazawa, Masao Ogura, Kazuki Tanaka, Shoji Kagami
Summary: Eculizumab demonstrated good efficacy and tolerability in pediatric patients with aHUS in a real-world setting in Japan. The treatment led to significant improvements in platelet count, lactate dehydrogenase, and estimated glomerular filtration rate, and achieved complete TMA response, hematologic normalization, and sCr decrease in a high percentage of patients.
NEPHROLOGY DIALYSIS TRANSPLANTATION
(2023)
Article
Biochemical Research Methods
Juri Sakata, Toshifumi Tatsumi, Akira Sugiyama, Akihiro Shimizu, Yuya Inagaki, Hiroto Katoh, Takefumi Yamashita, Kazuki Takahashi, Sho Aki, Yudai Kaneko, Takeshi Kawamura, Mai Miura, Masazumi Ishii, Tsuyoshi Osawa, Toshiya Tanaka, Shumpei Ishikawa, Masanobu Tsukagoshi, Michael Chansler, Tatsuhiko Kodama, Motomu Kanai, Hidetoshi Tokuyama, Kenzo Yamatsugu
Summary: This study reports an expression, refolding, and purification method for antibody-mimetic drug conjugate (AMDC). The AMDC uses a variable heavy chain of heavy chain-only antibodies (VHHs) as the targeting protein and non-covalently binds to a potent DNA-alkylating drug. The AMDC shows strong cytotoxic effects on cancer cells.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Yao Wang, Shaoting Weng, Yajie Tang, Sen Lin, Xiayue Liu, Wenhui Zhang, Gang Liu, Boomi Pandi, Yinrong Wu, Lei Ma, Lin Wang
Summary: In this study, a high yield of recombinant CD20 and claudin 18.2 proteins was achieved using an in vitro coupled transcription-translation system. The results showed that rituximab has a high affinity with CD20 protein. This study provides a novel concept for promoting the expression of multi-pass transmembrane proteins and lays the foundation for large-scale industrial production of membrane-associated drug targets.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Xiaobing Chen, Zijuan Zou, Wei Li, Xu Dong, Yi Chen, Yan Lu, Mingyue Zhu, Mengsen Li, Bo Lin
Summary: In this study, the fusion protein ImI-AFP3, composed of alpha-Conotoxin ImI and human alpha fetoprotein domain 3 (AFP3), was found to inhibit the growth and migration of lung cancer cells and showed synergistic effects with the drug gefitinib. These findings suggest that ImI-AFP3 is a promising candidate for the development of anticancer drugs.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Angel Castillo-Corujo, Mirva J. Saaranen, Lloyd W. Ruddock
Summary: This study successfully expressed two Fabs antibodies in the cytoplasm of E. coli using the CyDisCo system, achieving high yields and biological activity under industrially relevant fermentation conditions.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Junling Guo, Zhongyi Cheng, Zhemin Zhou
Summary: This study focused on a halophilic archaeal nitrile hydratase (NHase) and found that it exhibited higher tolerance to substrates and products compared to NHases from other sources. The unique genetic structure of this highly stable archaeal NHase could provide a theoretical foundation for modifying and enhancing the industrial application of NHase.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Yunlong Shen, Ruirui Zhang, Xiaohua Jiang, Jinliang Yang
Summary: The study developed a monoclonal antibody, B1M023, that can bind to LILRB1 with high affinity and block its binding to HLA-G. This antibody can promote the activation and IFN-gamma secretion of T cells, suggesting its potential applications in concomitant diagnosis and tumor immunotherapy.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Jun Jiang, Zhengqiang Jiang, Qiaojuan Yan, Susu Han, Shaoqing Yang
Summary: A novel alginate lyase gene from a marine bacterium showed efficient enzyme activity and stability, producing high conversion ratios of alginate oligosaccharides.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Xianghua Xiong, Yujin Qiu, Jiahao Zheng, Ling Zhou, Qingyang Wang, Jinglun Pang, Weicai Zhang, Huipeng Chen, Gang Liu, Xiaodong Han
Summary: A specific monoclonal antibody ML419 has been found to disrupt the recognition between Botulinum neurotoxin serotype A (BoNT/A) and FGFR3, effectively preventing BoNT/A from entering neurons. In vivo experiments show that ML419 has a strong protective effect, making it a promising candidate for the development of therapeutics against BoNT/A.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Maria Rain Jennings, Soohyon Min, Grace S. Xu, Kassandra Homayuni, Bhavana Suresh, Yusef Amir Haikal, John Blazeck
Summary: This study successfully produced Homo sapiens adenosine deaminase isoform 1 (HsADA1) and its variants through optimizing the recombinant expression process. The D8N variant of HsADA1 was found to be about 30% less active than the wildtype, but it better retained its activity in human serum. Additionally, the study revealed a previously undescribed phenomenon involving albumin that contributed to the increased activity of HsADA1 and the D8N variant in serum.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Oinam Sangita Devi, Senjam Sunil Singh, K. Rana, Sorokhaibam Jibankumar Singh, Wayenbam Sobhachandra Singh
Summary: A new lectin with hemagglutination activity was purified from the rhizome of Xanthosoma violaceum Schott. The lectin showed different reactions towards human red blood cells of different blood groups. It exhibited optimal hemagglutination activity at specific temperature and pH range, and showed good stability.
PROTEIN EXPRESSION AND PURIFICATION
(2024)