Article
Biotechnology & Applied Microbiology
Marcus A. Vinje, Cynthia A. Henson, Stanley H. Duke, Carl H. Simmons, Khoa Le, Evan Hall, Cory D. Hirsch
Summary: The study established an early model of the malting barley transcriptome, identified the period with the largest dynamic shift in gene expression during malting, and determined the expression patterns of starch degrading enzyme genes relevant to the malting and brewing industry. Large dynamic increases in gene expression occurred early in malting, with differentially expressed genes enriched for cell wall and starch hydrolases among other malting related categories.
Article
Food Science & Technology
Fengyu Guo, Jie An, Minlong Wang, Weibo Zhang, Chong Chen, Xueying Mao, Siyuan Liu, Pengjie Wang, Fazheng Ren
Summary: In this study, Quercimeritrin was identified as a selective inhibitor of alpha-glucosidase and shown to effectively control postprandial blood glucose in vivo. The results provide insights into using flavone aglycones to improve postprandial blood glucose levels.
Article
Chemistry, Applied
Fanny Coffigniez, Aurelien Briffaz
Summary: This study combined two previously developed modeling approaches to simulate the effect of key processing parameters on the nutritional value of cowpea seeds. The simulations showed that soaking and cooking processes were detrimental to folates, while germination promoted folate production and reduced the concentration of alpha-galactosides. The study suggests that a long germination period followed by a short cooking time is optimal in terms of both nutritional value and degree of cooking.
Article
Food Science & Technology
Waleed AL-Ansi, Yunji Wang, Mingcong Fan, Yan Li, Haifeng Qian, Li Wang
Summary: This study aimed to decrease the activity of phytohaemagglutinins and kidney bean a-amylase activity in some selected stable foods. Kidney bean a-amylase inhibitors may inhibit mammalian a-amylase, reducing the estimated glycaemic index (eGI). The results showed that the extract had a significant inhibitory effect on a-amylase activity and could decrease the eGI of various foods.
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Nutrition & Dietetics
Shengshu Xu, Likang Qin, Muhammad Mazhar, Yong Zhu
Summary: This study examined the phenolic profile, alpha-amylase inhibitor activities, starch composition, and glycemic index of seven varieties of kidney beans. The results showed that the colored beans contained more phenolics and had higher alpha-amylase inhibitor activities. The kidney beans starch demonstrated resistance to digestion, with a significant contribution of resistant starch to the low glycemic index. These findings suggest that kidney beans could be used as ingredients in functional low glycemic index foods.
FRONTIERS IN NUTRITION
(2022)
Article
Biochemistry & Molecular Biology
L. Viridiana Soto-Robles, Maria Fernanda Lopez, Veronica Torres-Banda, Claudia Cano-Ramirez, Gabriel Obregon-Molina, Gerardo Zuniga
Summary: Dendroctonus-bark beetles play important roles in forest ecosystems by colonizing and killing damaged pine trees. Research found that these beetles have an alpha-amylase capable of hydrolyzing starch, primarily active in the gut.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Medicinal
Bibi Fatima, Faiza Saleem, Uzma Salar, Sridevi Chigurupati, Shatha G. Felemban, Zaheer Ul-Haq, Syeda S. Tariq, Suliman A. Almahmoud, Muhammad Taha, Syed T. A. Shah, Khalid M. Khan
Summary: A library of 22 derivatives of 1,3,4-oxadiazole-2-thiol was synthesized and evaluated for its inhibitory potential against various enzymes and antioxidant activities. Most of the compounds showed good to moderate inhibition potential, with compound 3f exhibiting the highest inhibition against alpha-glucosidase and alpha-amylase enzymes.
ARCHIV DER PHARMAZIE
(2023)
Article
Biochemistry & Molecular Biology
Paola Jimenez-Alcantar, Rodolfo Lopez-Gomez, Joel E. Lopez-Meza, Alejandra Ochoa-Zarzosa
Summary: In recent years, the importance of epigenetic alterations in the development and maintenance of cancer, including leukemias, has been recognized. This study examines the cytotoxic effects of the plant antimicrobial peptide PaDef on acute lymphoid leukemia cells. It is found that PaDef induces cell death through an apoptotic process and there is an increase in reactive oxygen species production and loss of mitochondrial membrane potential. In addition, PaDef also induces epigenetic modifications in the Jurkat cells, such as increased histone acetylation and methylation. These findings suggest that PaDef could be a potential anticancer agent through epigenetic modulation.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Chemistry, Applied
Yi-Fan Tang, Wen -Xi Zhan, Mi-Zhuan Li, Lei Wang, Jie-Ying Wei, Jun -ling Deng, Zhong- Xiu Chen
Summary: Glutathione (GSH) can interact with starch through hydrogen bonds, accelerating starch granule swelling and inhibiting starch digestion. Incubation with GSH also leads to less stable conformation and decreased activity of pig pancreas a-amylase (PPA). However, in a crowded media with sodium caseinate (NaCas), an antagonistic effect between GSH and NaCas exists.
Article
Biochemistry & Molecular Biology
Marciele Souza da Silva, Valdirene Moreira Gomes, Gabriel Bonan Taveira, Layrana de Azevedo dos Santos, Alan C. Maracahipes, Rosana Rodrigues, Andre de Oliveira Carvalho, Katia Valevski S. Fernandes, Antonia Elenir A. Oliveira
Summary: The study evaluated the antimicrobial activity and mechanism of action of PEF2 fraction from Capsicum chinense Jack seeds against phytopathogenic fungi, demonstrating its ability to inhibit fungal growth, increase membrane permeability, and induce reactive oxygen species. These results may contribute to the biotechnological application of AMPs in controlling pathogenic microorganisms in agronomic plants.
PROTEIN AND PEPTIDE LETTERS
(2021)
Article
Chemistry, Applied
Wenyang Tao, Haibo Pan, He Jiang, Mengting Wang, Xingqian Ye, Shiguo Chen
Summary: Proanthocyanidins are flavan-3-ol polymers with multiple activities, but their extraction from fruits and seeds is costly. Extracting proanthocyanidins from trimmed leaves of fruit trees has economic benefits, with differences in structure and activity among various leaves.
Review
Microbiology
Xiaojing Wang, Jiaying Li, Jiaqi Shang, Jing Bai, Kai Wu, Jing Liu, Zhijun Yang, Hao Ou, Lei Shao
Summary: This review discusses the potential sources of alpha-glucosidase and alpha-amylase inhibitors from endophytes and marine microorganisms over the recent years, as well as the application of glycosidase inhibitors as drugs and dietary supplements. These studies are important for the future development of new microorganism-derived glycosidase inhibitors.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Jun-Jie Ke, Jing Lin, Xin Zhang, Xiao-Zheng Wu, Ying-Ying Zheng, Chun-Mei Hu, Yu Kang, Kun Zhang, Zhuang Xiong, Zhi-Qiang Ma
Summary: A series of benzylidene analogs of oleanolic acid were synthesized and evaluated for their inhibitory activities against alpha-glucosidase and alpha-amylase. The results showed that all synthesized analogs exhibited effective inhibitory effects and were safe for cells.
FRONTIERS IN CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Pukar Khanal, Prarambh S. R. Dwivedi, Vishal S. S. Patil, Ankith Shetty, S. Adithya, Afra Aga, R. Akshith, Aarif Javaid, Vishwambhar V. V. Bhandare
Summary: This study evaluated the inhibitory effects of barosmin on alpha-amylase, alpha-glucosidase, and dipeptidyl peptidase 4 enzymes, as well as its impact on glucose uptake efficacy. Results showed that barosmin formed stable nonbonded contacts with these enzymes and regulated the pathway related to glucose homeostasis. In vitro experiments also demonstrated the inhibitory activities of barosmin on these enzymes and its effect on glucose uptake in L6 cell lines. These findings highlight the potential of barosmin in anti-diabetic treatment.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Chemistry, Multidisciplinary
Mei Zhao, Limei Luo, Yongxian Guo, Binglu Zhao, Xiangfeng Chen, Xingang Shi, Mashooq Khan, Jin-Ming Lin, Qiongzheng Hu
Summary: This study presents a novel amylase sensor based on pH paper strips for quantitative detection of AMS. The sensor showed high sensitivity with a detection limit of 0.017 U/mL and excellent specificity. It was successfully used for distinguishing amylase levels in healthy individuals and acute pancreatitis patients.
Article
Agronomy
Nadia C. M. Silva, Jamile G. Conceicao, Kayan Eudorico Ventury, Leonardo F. R. De Sa, Eduardo A. G. Oliveira, Izabela S. Santos, Valdirene M. Gomes, Monique N. Costa, Andre T. S. Ferreira, Jonas Perales, Jose Xavier-Filho, Katia V. S. Fernandes, Antonia E. A. Oliveira
PEST MANAGEMENT SCIENCE
(2018)
Article
Plant Sciences
Priscila H. A. dos Santos, Izabela S. dos Santos, Vania M. M. Melo, Ilka M. Vasconcelos, Andre de O. Carvalho, Valdirene M. Gomes
ACTA PHYSIOLOGIAE PLANTARUM
(2010)
Article
Plant Sciences
Andre de O. Carvalho, Maura Da Cunha, Rosana Rodrigues, Claudia P. Sudre, Izabela S. Santos, Katia V. S. Fernandes, Guilherme R. Rabelo, Valdirene M. Gomes
ACTA PHYSIOLOGIAE PLANTARUM
(2011)
Article
Biochemistry & Molecular Biology
Umberto Zottich, Maura Da Cunha, Andre O. Carvalho, Germana B. Dias, Nadia C. M. Silva, Izabela S. Santos, Viviane V. do Nacimento, Emilio C. Miguel, Olga L. T. Machado, Valdirene M. Gomes
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2011)
Article
Biochemistry & Molecular Biology
Suzanna F. F. Ribeiro, Katia V. S. Fernandes, Izabela S. Santos, Gabriel B. Taveira, Andre O. Carvalho, Jose Luiz S. Lopes, Leila M. Beltramini, Rosana Rodrigues, Ilka M. Vasconcelos, Maura Da Cunha, Goncalo A. Souza-Filho, Valdirene M. Gomes
Article
Biochemistry & Molecular Biology
Erica de O. Mello, Izabela S. dos Santos, Andre de O. Carvalho, Luisa S. de Souza, Goncalo A. de Souza-Filho, Viviane V. do Nascimento, Olga L. T. Machado, Umberto Zottich, Valdirene M. Gomes
Article
Microbiology
Erica O. Mello, Suzanna F. F. Ribeiro, Andre O. Carvalho, Izabela S. Santos, Maura Da Cunha, Claudete Santa-Catarina, Valdirene M. Gomes
CURRENT MICROBIOLOGY
(2011)
Article
Agriculture, Multidisciplinary
Janne Keila S. Morais, Valdirene M. Gomes, Jose Tadeu A. Oliveira, Izabela S. Santos, Maura Da Cunha, Hermogenes D. Oliveira, Henrique P. Oliveira, Daniele O. B. Sousa, Ilka M. Vasconcelos
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2010)
Article
Entomology
P. O. Santos, I. S. Santos, V. M. Gomes, O. L. T. Machado, Kv. S. Fernandes, J. Xavier-Filho, A. E. A. Oliveira
JOURNAL OF STORED PRODUCTS RESEARCH
(2008)
Article
Biochemistry & Molecular Biology
Patricia D. Games, Izabela S. dos Santos, Erica O. Mello, Mariangela S. S. Diz, Andre O. Carvalho, Goncalo A. de Souza-Filho, Maura Da Cunha, Ilka M. Vasconcelos, Beatriz dos S. Ferreira, Valdirene M. Gomes
Article
Biochemistry & Molecular Biology
Julia Ribeiro Soares, Andre de Oliveira Carvalho, Izabela Silva dos Santos, Olga Lima Tavares Machado, Viviane V. Nascimento, Ilka Maria Vasconcelos, Andre Teixeira da Silva Ferreira, Jonas Enrique de Aguilar Perales, Valdirene Moreira Gomes
PROTEIN AND PEPTIDE LETTERS
(2012)
Article
Plant Sciences
Izabela S. Santos, Antonia E. A. Oliveira, Maura Da Cunhac, Olga L. T. Machado, Ana G. C. Neves-Ferreira, Katia V. S. Fernandes, Andre O. Carvalho, Jonas Perales, Valdirene M. Gomes
PHYSIOLOGIA PLANTARUM
(2007)
Article
Agronomy
FM Nóbrega, IS Santos, M Da Cunha, AO Carvalho, VM Gomes
Article
Biochemistry & Molecular Biology
IS Santos, M Da Cunha, OLT Machado, VM Gomes
Article
Biochemical Research Methods
Juri Sakata, Toshifumi Tatsumi, Akira Sugiyama, Akihiro Shimizu, Yuya Inagaki, Hiroto Katoh, Takefumi Yamashita, Kazuki Takahashi, Sho Aki, Yudai Kaneko, Takeshi Kawamura, Mai Miura, Masazumi Ishii, Tsuyoshi Osawa, Toshiya Tanaka, Shumpei Ishikawa, Masanobu Tsukagoshi, Michael Chansler, Tatsuhiko Kodama, Motomu Kanai, Hidetoshi Tokuyama, Kenzo Yamatsugu
Summary: This study reports an expression, refolding, and purification method for antibody-mimetic drug conjugate (AMDC). The AMDC uses a variable heavy chain of heavy chain-only antibodies (VHHs) as the targeting protein and non-covalently binds to a potent DNA-alkylating drug. The AMDC shows strong cytotoxic effects on cancer cells.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Yao Wang, Shaoting Weng, Yajie Tang, Sen Lin, Xiayue Liu, Wenhui Zhang, Gang Liu, Boomi Pandi, Yinrong Wu, Lei Ma, Lin Wang
Summary: In this study, a high yield of recombinant CD20 and claudin 18.2 proteins was achieved using an in vitro coupled transcription-translation system. The results showed that rituximab has a high affinity with CD20 protein. This study provides a novel concept for promoting the expression of multi-pass transmembrane proteins and lays the foundation for large-scale industrial production of membrane-associated drug targets.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Xiaobing Chen, Zijuan Zou, Wei Li, Xu Dong, Yi Chen, Yan Lu, Mingyue Zhu, Mengsen Li, Bo Lin
Summary: In this study, the fusion protein ImI-AFP3, composed of alpha-Conotoxin ImI and human alpha fetoprotein domain 3 (AFP3), was found to inhibit the growth and migration of lung cancer cells and showed synergistic effects with the drug gefitinib. These findings suggest that ImI-AFP3 is a promising candidate for the development of anticancer drugs.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Angel Castillo-Corujo, Mirva J. Saaranen, Lloyd W. Ruddock
Summary: This study successfully expressed two Fabs antibodies in the cytoplasm of E. coli using the CyDisCo system, achieving high yields and biological activity under industrially relevant fermentation conditions.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Junling Guo, Zhongyi Cheng, Zhemin Zhou
Summary: This study focused on a halophilic archaeal nitrile hydratase (NHase) and found that it exhibited higher tolerance to substrates and products compared to NHases from other sources. The unique genetic structure of this highly stable archaeal NHase could provide a theoretical foundation for modifying and enhancing the industrial application of NHase.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Yunlong Shen, Ruirui Zhang, Xiaohua Jiang, Jinliang Yang
Summary: The study developed a monoclonal antibody, B1M023, that can bind to LILRB1 with high affinity and block its binding to HLA-G. This antibody can promote the activation and IFN-gamma secretion of T cells, suggesting its potential applications in concomitant diagnosis and tumor immunotherapy.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Jun Jiang, Zhengqiang Jiang, Qiaojuan Yan, Susu Han, Shaoqing Yang
Summary: A novel alginate lyase gene from a marine bacterium showed efficient enzyme activity and stability, producing high conversion ratios of alginate oligosaccharides.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Xianghua Xiong, Yujin Qiu, Jiahao Zheng, Ling Zhou, Qingyang Wang, Jinglun Pang, Weicai Zhang, Huipeng Chen, Gang Liu, Xiaodong Han
Summary: A specific monoclonal antibody ML419 has been found to disrupt the recognition between Botulinum neurotoxin serotype A (BoNT/A) and FGFR3, effectively preventing BoNT/A from entering neurons. In vivo experiments show that ML419 has a strong protective effect, making it a promising candidate for the development of therapeutics against BoNT/A.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Maria Rain Jennings, Soohyon Min, Grace S. Xu, Kassandra Homayuni, Bhavana Suresh, Yusef Amir Haikal, John Blazeck
Summary: This study successfully produced Homo sapiens adenosine deaminase isoform 1 (HsADA1) and its variants through optimizing the recombinant expression process. The D8N variant of HsADA1 was found to be about 30% less active than the wildtype, but it better retained its activity in human serum. Additionally, the study revealed a previously undescribed phenomenon involving albumin that contributed to the increased activity of HsADA1 and the D8N variant in serum.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Oinam Sangita Devi, Senjam Sunil Singh, K. Rana, Sorokhaibam Jibankumar Singh, Wayenbam Sobhachandra Singh
Summary: A new lectin with hemagglutination activity was purified from the rhizome of Xanthosoma violaceum Schott. The lectin showed different reactions towards human red blood cells of different blood groups. It exhibited optimal hemagglutination activity at specific temperature and pH range, and showed good stability.
PROTEIN EXPRESSION AND PURIFICATION
(2024)