Article
Chemistry, Multidisciplinary
Jing Sun, Min Li, Bo Zhang, Xuesi Chen
Summary: This study presents an efficient strategy for synthesizing antibacterial polypeptoid sulfonium ions through ring-opening polymerization and post-polymerization functionalization. Systematic investigations were conducted to explore the impact of hydrophobic/hydrophilic balance on antimicrobial activity and selectivity. The optimized amphiphilic balance in the chloroepoxypropane-modified polysulfoniums showed higher selectivity towards bacteria and great efficiency in inhibiting biofilm formations.
ADVANCED MATERIALS
(2021)
Article
Biochemistry & Molecular Biology
Chih-Chien Hu, Selvaraj Rajesh Kumar, Truong Thi Tuong Vi, Yu-Tzu Huang, Dave W. Chen, Shingjiang Jessie Lue
Summary: In this study, the antimicrobial peptide GL13K was coated onto a PEEK substrate, resulting in significant antibacterial and biofilm-inhibitory effects against Staphylococcus aureus bacteria. The coating technique used in this study holds promise for providing bactericidal activity and biofilm resistance to medical implantable devices.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Jie Chen, You-Fu Lin, Jia-Hao Chen, Xiang Chen, Zhi-Hua Lin
Summary: The study revealed that tiger frog Cathelicidin has high antimicrobial activity, with the mature peptide showing high variability in amphibians while the cathelin domain remains conserved.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Kunzhong Guo, Minjie Zhang, Junyi Cai, Zunwei Ma, Zhou Fang, Haiyan Zhou, Junjian Chen, Meng Gao, Lin Wang
Summary: In this study, AIE nanofibers were developed with precisely adjustable antibacterial activity by changing the decorated peptides. Mechanistic analysis revealed that the synergistic antibacterial activities of ROS and peptides contributed to this effect. The optimized nanofibers showed excellent antibacterial activity in vitro and in vivo, offering new opportunities for photodynamic therapy of infection.
Article
Chemistry, Medicinal
Manuel K. Langer, Ataur Rahman, Hymonti Dey, Trude Anderssen, Francesco Zilioli, Tor Haug, Hans -Matti Blencke, Klara Stensvag, Morten B. Strom, Annette Bayer
Summary: This study reports a promising class of antimicrobial agents, amphipathic barbiturate mimics of marine eusynstyelamides. A detailed analysis of the structure-activity relationship for cationic amphipathic barbiturates was conducted, and it was found that different cationic groups, hydrocarbon linkers, and lipophilic side chains had varying effects on their antimicrobial potency and haemolytic activity. Based on these findings, both potent narrow-spectrum and broad-spectrum barbiturates with low or no haemolytic activity were successfully prepared.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Biotechnology & Applied Microbiology
Chunming Dong, Lijun Xu, Weitao Lu, Mengru Li, Rui Zhang, Yanyan Sun, Jian Liu, Xinlei Chu
Summary: Researchers expressed a recombinant PMAP-37(F34-R) using Pichia pastoris and found that it exhibited a broad-spectrum antibacterial activity against both Gram-positive and Gram-negative bacteria. The peptide had a minimum inhibitory concentration of 0.12-0.24 μg/mL and showed good stability. Additionally, PMAP-37(F34-R) had low hemolysis ability to mammalian cells and demonstrated a good preservative effect in plums.
MICROBIAL CELL FACTORIES
(2023)
Article
Food Science & Technology
Shen Yang, Mingyao Wang, Jialong Gao, Jiaxin Liu, Ritian Jin, Rong Lin, Wuyin Weng, Jude Juventus Aweya
Summary: The activity of antimicrobial peptides (AMPs) can be enhanced by low concentration of sodium chloride (NaCl), which can increase the antibacterial effect against Staphylococcus aureus. The enhancement is achieved through the destruction of bacterial structure, transformation of peptide structure, and increased binding and permeability to bacterial membranes.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Engineering, Biomedical
Wenhao Zhou, Tian Bai, Lan Wang, Yan Cheng, Dandan Xia, Sen Yu, Yufeng Zheng
Summary: The combination of antimicrobial peptides and silver nanoparticles in a silk fibroin-based coating showed excellent infection-triggered antibacterial and osteointegration properties. The coating displayed sensitive pH-responsive behavior and a high antibacterial rate. In vivo experiments demonstrated enhanced osseointegration compared to untreated titanium implants.
BIOACTIVE MATERIALS
(2023)
Article
Engineering, Biomedical
Hui Shao, Jin Zhou, Xiaoqian Lin, Yue Zhou, Yumeng Xue, Weili Hong, Xubo Lin, Xiaoling Jia, Yubo Fan
Summary: Developing new antimicrobial agents to combat drug-resistant pathogens and biofilms is an urgent task. Cationic antimicrobial peptides (AMPs) have potential due to their unique membrane rupture mechanism, but their high toxicity and low stability limit their application. In this study, a biomimetic strategy was used to construct cationic peptide-conjugated liposomes, inspired by cell-penetrating peptides (CPPs), to enhance antibacterial efficacy and biosafety. Computational simulation and experimental investigations identified the optimal peptide-conjugated liposomes, which showed enhanced antibacterial efficacy without compromising cytotoxicity. This bio-inspired design could contribute to the development of next-generation antimicrobials.
Article
Biochemistry & Molecular Biology
Sara Emamifar, Shamsozoha Abolmaali, Seyyed Mohsen Sohrabi, Mohsen Mohammadi, Maasume Shahmohammadi
Summary: In this study, an antibacterial plant defensin peptide, AsDef1, was successfully identified and characterized from oat. AsDef1 exhibited broad-spectrum antibacterial activity, with the highest expression in developing seeds of the plant. The findings suggest AsDef1 could be used for producing pathogen-resistant transgenic plants and potential antibacterial agents in the future.
Article
Pharmacology & Pharmacy
Tengfei Shen, Liangliang Chen, Yongqing Liu, Shuaibing Shi, Zhixin Liu, Kairui Cai, Chengshui Liao, Chen Wang
Summary: PMAP-23RI-Dec demonstrated high antibacterial activity and stability, affecting biofilm and cell membranes, while not increasing hemolytic activity and cytotoxicity. It reduced organ damage by bacteria and showed superior efficacy in treating peritonitis and alleviating wound infections.
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Melaine Gonzalez-Garcia, Fidel Morales-Vicente, Erbio Diaz Pico, Hilda Garay, Daniel G. Rivera, Mark Grieshober, Lia Raluca Olari, Rudiger Gross, Carina Conzelmann, Franziska Krueger, Fabian Zech, Caterina Prelli Bozzo, Janis A. Mueller, Alexander Zelikin, Heinz Raber, Dennis Kubiczek, Frank Rosenau, Jan Muench, Steffen Stenger, Barbara Spellerberg, Octavio L. Franco, Armando A. Rodriguez Alfonso, Ludger Staendker, Anselmo J. Otero-Gonzalez
Summary: Cm-p5 and its derivatives showed moderate antimicrobial activity against certain bacteria and viruses, indicating potential applications but requiring further structural optimization.
Article
Fisheries
Shui-Sheng Yu, Zeng-Hui Zhao, Xiao-Fei Gong, Xiao-Li Fan, Zhi-Hua Lin, Jie Chen
Summary: The study identified an 8-defensin cDNA from the skin transcriptome of Chinese spiny frog, Quasipaa spinosa, which showed antibacterial activity against various bacteria. Synthetic QS-BD demonstrated bactericidal activity by destroying cell membrane integrity. This is the first evidence of immunomodulatory activity by an amphibian 8-defensin.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2022)
Article
Fisheries
Zhuo-Hao Ruan, Wen Huang, Yi-Fu Li, Liang-Sen Jiang, Zhi-Qiang Lu, Yuan-Yuan Luo, Xi-Quan Zhang, Wen-Sheng Liu
Summary: This study cloned and characterized the cDNA sequence of the NK-lysin gene in striped catfish, and found that it had antibacterial properties. The transcriptional levels of PhNK-lysin were significantly increased in response to A. hydrophila infection in various tissues. The synthetic PhNK-lysin-derived peptide showed antibacterial activity against Vibrio harveii, Aeromonas hydrophila and Escherichia coli. Moreover, the synthetic PhNK-lysin reduced the bacterial load in the liver and trunk kidney of infected striped catfish, and increased their survival rate.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Microbiology
Guoyu Li, Xiaojie Yuan, Hongyu Chen, Bowen Li, Changxuan Shao, Yongjie Zhu, Zhenheng Lai, Anshan Shan
Summary: This study investigates the effect of systematic mutations in the hydrogen bond formation site of an alpha-helical antimicrobial peptide on its activity and cell selectivity. The optimized peptide showed excellent antibacterial ability and selectivity, and significantly reduced bacterial loads in vivo. These findings contribute to the design and optimization of highly active and low-toxicity antimicrobial peptides.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemical Research Methods
Juri Sakata, Toshifumi Tatsumi, Akira Sugiyama, Akihiro Shimizu, Yuya Inagaki, Hiroto Katoh, Takefumi Yamashita, Kazuki Takahashi, Sho Aki, Yudai Kaneko, Takeshi Kawamura, Mai Miura, Masazumi Ishii, Tsuyoshi Osawa, Toshiya Tanaka, Shumpei Ishikawa, Masanobu Tsukagoshi, Michael Chansler, Tatsuhiko Kodama, Motomu Kanai, Hidetoshi Tokuyama, Kenzo Yamatsugu
Summary: This study reports an expression, refolding, and purification method for antibody-mimetic drug conjugate (AMDC). The AMDC uses a variable heavy chain of heavy chain-only antibodies (VHHs) as the targeting protein and non-covalently binds to a potent DNA-alkylating drug. The AMDC shows strong cytotoxic effects on cancer cells.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Yao Wang, Shaoting Weng, Yajie Tang, Sen Lin, Xiayue Liu, Wenhui Zhang, Gang Liu, Boomi Pandi, Yinrong Wu, Lei Ma, Lin Wang
Summary: In this study, a high yield of recombinant CD20 and claudin 18.2 proteins was achieved using an in vitro coupled transcription-translation system. The results showed that rituximab has a high affinity with CD20 protein. This study provides a novel concept for promoting the expression of multi-pass transmembrane proteins and lays the foundation for large-scale industrial production of membrane-associated drug targets.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Xiaobing Chen, Zijuan Zou, Wei Li, Xu Dong, Yi Chen, Yan Lu, Mingyue Zhu, Mengsen Li, Bo Lin
Summary: In this study, the fusion protein ImI-AFP3, composed of alpha-Conotoxin ImI and human alpha fetoprotein domain 3 (AFP3), was found to inhibit the growth and migration of lung cancer cells and showed synergistic effects with the drug gefitinib. These findings suggest that ImI-AFP3 is a promising candidate for the development of anticancer drugs.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Angel Castillo-Corujo, Mirva J. Saaranen, Lloyd W. Ruddock
Summary: This study successfully expressed two Fabs antibodies in the cytoplasm of E. coli using the CyDisCo system, achieving high yields and biological activity under industrially relevant fermentation conditions.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Junling Guo, Zhongyi Cheng, Zhemin Zhou
Summary: This study focused on a halophilic archaeal nitrile hydratase (NHase) and found that it exhibited higher tolerance to substrates and products compared to NHases from other sources. The unique genetic structure of this highly stable archaeal NHase could provide a theoretical foundation for modifying and enhancing the industrial application of NHase.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Yunlong Shen, Ruirui Zhang, Xiaohua Jiang, Jinliang Yang
Summary: The study developed a monoclonal antibody, B1M023, that can bind to LILRB1 with high affinity and block its binding to HLA-G. This antibody can promote the activation and IFN-gamma secretion of T cells, suggesting its potential applications in concomitant diagnosis and tumor immunotherapy.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Jun Jiang, Zhengqiang Jiang, Qiaojuan Yan, Susu Han, Shaoqing Yang
Summary: A novel alginate lyase gene from a marine bacterium showed efficient enzyme activity and stability, producing high conversion ratios of alginate oligosaccharides.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Xianghua Xiong, Yujin Qiu, Jiahao Zheng, Ling Zhou, Qingyang Wang, Jinglun Pang, Weicai Zhang, Huipeng Chen, Gang Liu, Xiaodong Han
Summary: A specific monoclonal antibody ML419 has been found to disrupt the recognition between Botulinum neurotoxin serotype A (BoNT/A) and FGFR3, effectively preventing BoNT/A from entering neurons. In vivo experiments show that ML419 has a strong protective effect, making it a promising candidate for the development of therapeutics against BoNT/A.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Maria Rain Jennings, Soohyon Min, Grace S. Xu, Kassandra Homayuni, Bhavana Suresh, Yusef Amir Haikal, John Blazeck
Summary: This study successfully produced Homo sapiens adenosine deaminase isoform 1 (HsADA1) and its variants through optimizing the recombinant expression process. The D8N variant of HsADA1 was found to be about 30% less active than the wildtype, but it better retained its activity in human serum. Additionally, the study revealed a previously undescribed phenomenon involving albumin that contributed to the increased activity of HsADA1 and the D8N variant in serum.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Oinam Sangita Devi, Senjam Sunil Singh, K. Rana, Sorokhaibam Jibankumar Singh, Wayenbam Sobhachandra Singh
Summary: A new lectin with hemagglutination activity was purified from the rhizome of Xanthosoma violaceum Schott. The lectin showed different reactions towards human red blood cells of different blood groups. It exhibited optimal hemagglutination activity at specific temperature and pH range, and showed good stability.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
