Article
Immunology
Ika Nurzijah, Ola A. Elbohy, Kostya Kanyuka, Janet M. Daly, Stephen Dunham
Summary: Plant-based vaccines provide a new feasibility for preventing avian influenza and Newcastle disease, with the advantages of high efficiency, stability, and safety. Compared to traditional vaccines, plant-derived virus-like particle vaccines have significant benefits, reducing virus shedding and distinguishing between infected and vaccinated birds.
Review
Oncology
Jennifer Stander, Sandiswa Mbewana, Ann E. Meyers
Summary: The idea of producing vaccines in plants originated in the late 1980s, focusing on edible vaccines at first. However, due to issues with antigen expression, administration difficulties, and regulatory rules for transgenic plants, purified vaccines administered parenterally have become more common. With improved expression techniques and a deeper understanding of biological processes, plant-produced vaccines have been successful, leading to the development of various types of vaccine candidates.
Article
Biology
Gergana Zahmanova, Katerina Takova, Rumyana Valkova, Valentina Toneva, Ivan Minkov, Anton Andonov, Georgi L. Lukov
Summary: Vaccination is the most effective way to restrict the spread of zoonotic viruses and prevent diseases. Recombinant proteins produced in plants offer a safe, effective, and inexpensive approach for vaccine development. Virus-like particles (VLPs) produced in plants, which can self-assemble and resemble the form of virus particles, are gaining popularity as candidate vaccines against various infectious diseases.
Review
Veterinary Sciences
Zenglei Hu, Xiaozheng He, Jing Deng, Jiao Hu, Xiufan Liu
Summary: Newcastle disease is a highly economically damaging infectious disease affecting poultry, causing severe tissue damage in multiple systems. Progress has been made in preventing morbidity and mortality caused by the virus. However, the virus is still endemic in some regions, impacting the livelihoods of people dependent on poultry for food.
VETERINARY RESEARCH
(2022)
Article
Plant Sciences
Tanja Smith, Martha M. O'Kennedy, Craig S. Ross, Nicola S. Lewis, Celia Abolnik
Summary: In this study, an expression platform was established to produce Newcastle disease virus-like particles (VLPs) for use as vaccines. The ND Fusion (F) and Hemagglutinin-neuraminidase (HN) proteins of a genotype VII.2 strain formed ND VLPs in plants and agglutinated chicken erythrocytes. The partially-purified ND VLPs showed immunogenicity and successfully inhibited viral replication in vitro. These plant-produced ND VLPs have great potential as antigen-matched vaccines for poultry and other avian species.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Immunology
Nabila Osman, Danny Goovaerts, Serageldeen Sultan, Jeremy Salt, Christian Grund
Summary: The commercial I-2 strains showed superior thermal stability at 37 degrees Celsius compared to other ND vaccines, but all vaccines dissolved at 51 and 61 degrees Celsius within 2 to 4 hours. This study highlights the importance of vaccine formulation-specific factors and cold chain maintenance for vaccine efficacy in hot climates.
Article
Immunology
Hesham A. Sultan, Wael K. Elfeil, Ahmed A. Nour, Laila Tantawy, Elsayed G. Kamel, Emad M. Eed, Ahmad El Askary, Shaimaa Talaat
Summary: Class II genotype VII Newcastle disease viruses (NDV) are prevalent in the Middle East and Asia despite extensive vaccination efforts. This study evaluated the protective efficacy of three different commercial vaccine regimes against challenge with a virulent NDV genotype VII strain. The results indicate that using closely genotype-matched vaccines provides higher protection against challenge compared to using non-matched or non-genotype-matched vaccines. Regular updating of vaccine seeds with closely related isolates is crucial for enhancing protection levels.
Article
Immunology
Elizabeth Monreal-Escalante, Abel Ramos-Vega, Carlos Angulo, Bernardo Banuelos-Hernandez
Summary: This review article describes the use of plants as biofactories for vaccine production. Only a few plant-produced vaccines have been evaluated in clinical trials, with COVID-19 vaccines being the most recent ones approved by the FDA. The article also discusses the main strategies for antigen design and high antigen production levels, along with providing perspectives.
Review
Pharmacology & Pharmacy
Luis Alberto Bravo-Vazquez, Erick Octavio Mora-Hernandez, Alma L. L. Rodriguez, Padmavati Sahare, Anindya Bandyopadhyay, Asim K. K. Duttaroy, Sujay Paul
Summary: Neurodegenerative diseases (NDDs) are a major global health issue characterized by the loss of neurons in the central nervous system. Immunotherapeutic approaches using plant-based antigen expression systems have shown promising results in treating NDDs such as Alzheimer's, Parkinson's, and multiple sclerosis. These plant-made vaccines induce significant immune responses and offer advantages like scalability, absence of human pathogens, cold chain-free production, and lower costs. This article provides an overview of the progress and future prospects of plant-manufactured vaccines for NDDs.
Review
Plant Sciences
Srividhya Venkataraman, Imran Khan, Peyman Habibi, Maria Le, Rory Lippert, Kathleen Hefferon
Summary: Plants have been explored as a platform for producing pharmaceutical proteins for over 20 years. This review article focuses on the expression and downstream purification processes for plant-made vaccines. The advantages of using plants for vaccine production, such as cost-effectiveness, ease of scaling up, and the ability to produce complex therapeutic proteins, are discussed.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Veterinary Sciences
Putri Pandarangga, Milton M. McAllister, Anne E. Peaston, Yuen T. Ngai, Mohammad I. Cahyono, Farhid Hemmatzadeh
Summary: Antigenic differences between commercial Newcastle Disease Virus (NDV) vaccine and circulating field virus reduce vaccine efficacy. Superior results are obtained when serological testing, vaccinations, and vaccine challenge experiments all use circulating strains of ND virus.
RESEARCH IN VETERINARY SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
Yonghua Li, Zaib Ur Rehman, Mengjiao Li, Zahid Manzoor, Wei Liu, Xusheng Qiu, Yingjie Sun, Ying Liao, Lei Tan, Cuiping Song, Weiwei Liu, Shengqing Yu, Chan Ding, Chunchun Meng
Summary: The study found that under the pressure of genotype II and VII anti-NDV serum, variations in the F and HN genes of different NDV strains occurred, but there were no significant differences between the two serum groups.
Article
Immunology
Julia Puppin Chaves Fulber, Omar Farnos, Sascha Kiesslich, Zeyu Yang, Shantoshini Dash, Leonardo Susta, Sarah K. Wootton, Amine A. Kamen
Summary: The study successfully transferred NDV production to cell culture, developing methods to replicate NDV in Vero and HEK293 suspension cultures and produce high viral titers. This lays the foundation for a scalable vaccine manufacturing process.
Article
Biology
Adnan Raza, Muhammad Asif Rasheed, Sohail Raza, Muhammad Tariq Navid, Amna Afzal, Farrukh Jamil
Summary: A multi-epitope vaccine against 26 strains of Newcastle disease virus from Pakistan was designed using immunoinformatic approaches. The vaccine exhibited high immunogenicity, stability, and potential to induce a strong immune response.
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Immunology
Caterina Lupini, Matteo Legnardi, Giulia Graziosi, Mattia Cecchinato, Valeria Listorti, Calogero Terregino, Elena Catelli
Summary: Newcastle disease virus (NDV) and avian metapneumovirus (aMPV) are highly impactful pathogens in the turkey industry. Although the compatibility of their vaccines has not been experimentally confirmed in turkeys, administering aMPV live vaccine with ND vaccines has shown positive results in terms of protection against aMPV.
Article
Biochemical Research Methods
Juri Sakata, Toshifumi Tatsumi, Akira Sugiyama, Akihiro Shimizu, Yuya Inagaki, Hiroto Katoh, Takefumi Yamashita, Kazuki Takahashi, Sho Aki, Yudai Kaneko, Takeshi Kawamura, Mai Miura, Masazumi Ishii, Tsuyoshi Osawa, Toshiya Tanaka, Shumpei Ishikawa, Masanobu Tsukagoshi, Michael Chansler, Tatsuhiko Kodama, Motomu Kanai, Hidetoshi Tokuyama, Kenzo Yamatsugu
Summary: This study reports an expression, refolding, and purification method for antibody-mimetic drug conjugate (AMDC). The AMDC uses a variable heavy chain of heavy chain-only antibodies (VHHs) as the targeting protein and non-covalently binds to a potent DNA-alkylating drug. The AMDC shows strong cytotoxic effects on cancer cells.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Yao Wang, Shaoting Weng, Yajie Tang, Sen Lin, Xiayue Liu, Wenhui Zhang, Gang Liu, Boomi Pandi, Yinrong Wu, Lei Ma, Lin Wang
Summary: In this study, a high yield of recombinant CD20 and claudin 18.2 proteins was achieved using an in vitro coupled transcription-translation system. The results showed that rituximab has a high affinity with CD20 protein. This study provides a novel concept for promoting the expression of multi-pass transmembrane proteins and lays the foundation for large-scale industrial production of membrane-associated drug targets.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Xiaobing Chen, Zijuan Zou, Wei Li, Xu Dong, Yi Chen, Yan Lu, Mingyue Zhu, Mengsen Li, Bo Lin
Summary: In this study, the fusion protein ImI-AFP3, composed of alpha-Conotoxin ImI and human alpha fetoprotein domain 3 (AFP3), was found to inhibit the growth and migration of lung cancer cells and showed synergistic effects with the drug gefitinib. These findings suggest that ImI-AFP3 is a promising candidate for the development of anticancer drugs.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Angel Castillo-Corujo, Mirva J. Saaranen, Lloyd W. Ruddock
Summary: This study successfully expressed two Fabs antibodies in the cytoplasm of E. coli using the CyDisCo system, achieving high yields and biological activity under industrially relevant fermentation conditions.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Junling Guo, Zhongyi Cheng, Zhemin Zhou
Summary: This study focused on a halophilic archaeal nitrile hydratase (NHase) and found that it exhibited higher tolerance to substrates and products compared to NHases from other sources. The unique genetic structure of this highly stable archaeal NHase could provide a theoretical foundation for modifying and enhancing the industrial application of NHase.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Yunlong Shen, Ruirui Zhang, Xiaohua Jiang, Jinliang Yang
Summary: The study developed a monoclonal antibody, B1M023, that can bind to LILRB1 with high affinity and block its binding to HLA-G. This antibody can promote the activation and IFN-gamma secretion of T cells, suggesting its potential applications in concomitant diagnosis and tumor immunotherapy.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Jun Jiang, Zhengqiang Jiang, Qiaojuan Yan, Susu Han, Shaoqing Yang
Summary: A novel alginate lyase gene from a marine bacterium showed efficient enzyme activity and stability, producing high conversion ratios of alginate oligosaccharides.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Xianghua Xiong, Yujin Qiu, Jiahao Zheng, Ling Zhou, Qingyang Wang, Jinglun Pang, Weicai Zhang, Huipeng Chen, Gang Liu, Xiaodong Han
Summary: A specific monoclonal antibody ML419 has been found to disrupt the recognition between Botulinum neurotoxin serotype A (BoNT/A) and FGFR3, effectively preventing BoNT/A from entering neurons. In vivo experiments show that ML419 has a strong protective effect, making it a promising candidate for the development of therapeutics against BoNT/A.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Maria Rain Jennings, Soohyon Min, Grace S. Xu, Kassandra Homayuni, Bhavana Suresh, Yusef Amir Haikal, John Blazeck
Summary: This study successfully produced Homo sapiens adenosine deaminase isoform 1 (HsADA1) and its variants through optimizing the recombinant expression process. The D8N variant of HsADA1 was found to be about 30% less active than the wildtype, but it better retained its activity in human serum. Additionally, the study revealed a previously undescribed phenomenon involving albumin that contributed to the increased activity of HsADA1 and the D8N variant in serum.
PROTEIN EXPRESSION AND PURIFICATION
(2024)
Article
Biochemical Research Methods
Oinam Sangita Devi, Senjam Sunil Singh, K. Rana, Sorokhaibam Jibankumar Singh, Wayenbam Sobhachandra Singh
Summary: A new lectin with hemagglutination activity was purified from the rhizome of Xanthosoma violaceum Schott. The lectin showed different reactions towards human red blood cells of different blood groups. It exhibited optimal hemagglutination activity at specific temperature and pH range, and showed good stability.
PROTEIN EXPRESSION AND PURIFICATION
(2024)