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Polysaccharide co-polymerases: the enigmatic conductors of the O-antigen assembly orchestra

PROTEIN ENGINEERING DESIGN & SELECTION (2012)

期刊

PROTEIN ENGINEERING DESIGN & SELECTION

卷 25, 期 11, 页码 797-802

出版社

OXFORD UNIV PRESS

DOI: 10.1093/protein/gzs075

关键词

crystal structure; LPS synthesis; O-antigens; polysaccharides

类别

Biochemistry & Molecular Biology Biotechnology & Applied Microbiology

资金

Canadian Institutes of Health Research (CIHR) [MOP-89787]

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Protocol

Reagent

摘要

The O-antigen lipopolysaccharides on bacterial surface contain variable number of oligosaccharide repeat units with their length having a modal distribution specific to the bacterial strain. The polysaccharide length distribution is controlled by the proteins called polysaccharide co-polymerases (PCPs), which are embedded in the inner membrane in Gram-negative bacteria and form homo oligomers. The 3D structures of periplasmic domains of several PCPs have been determined and provided the first insights into the possible mechanism of polysaccharide length determination mechanism. Here we review the current knowledge of structure and function of these polysaccharide length regulators.

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