Article
Biochemistry & Molecular Biology
Kun-Hua Yu, Mei-Yu Huang, Yi-Ru Lee, Yu-Kie Lin, Hau-Ren Chen, Cheng- Lee
Summary: Octapeptide repeats play a crucial role in the folding and misfolding of PrP, influencing the formation of amyloid fibrils with distinct structures and impacting the cytotoxicity in the onset of prion diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Analytical
Zhuoyu Liu, Yanjing Cao, Lei Zhang, Ya Xu, Zhongli Zhang
Summary: A novel MDLC-MS method was developed for charge variant characterization in therapeutic antibody development, aiming to discover potential risks on safety and efficacy. The method enabled detection of charged species at low abundance by implementing 96-well plate fractionation and on-column preconcentration. The charge variants of mAb-A were characterized, and post-translational modifications were identified and evaluated for their potential risks. The MDLC-MS detection demonstrated sample-economic, time-effective, and labor-saving features, providing a powerful and timely tool for charge variant characterization in antibody development.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Sean M. Cascarina, Joshua P. Kaplan, Mikaela R. Elder, Lindsey Brookbank, Eric D. Ross
Summary: The degradation sensitivity of G-rich and Q/N-rich domains is influenced by specific sequence features, with G-rich domains being more susceptible to degradation-promoting effects of hydrophobic residues. The proteostasis network may act as a selection mechanism at the molecular level, constraining the sequence space accessible to G-rich domains. However, the sensitivity or resistance of these domains is not always preserved in their native protein contexts, indicating the complexity of protein evolution in overcoming degradation susceptibility.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Analytical
Oladapo Olaleye, Baubek Spanov, Robert Ford, Natalia Govorukhina, Nico C. van de Merbel, Rainer Bischoff
Summary: Trastuzumab and pertuzumab, monoclonal antibodies used in treating HER2-positive breast cancer, were studied for in vivo biotransformation using Affimer reagents for their enrichment from plasma. The experiments were conducted under simulated in vivo conditions for stress testing of the antibodies.
ANALYTICAL CHEMISTRY
(2021)
Review
Neurosciences
Stefano Thellung, Alessandro Corsaro, Irene Dellacasagrande, Mario Nizzari, Martina Zambito, Tullio Florio
Summary: Transmissible spongiform encephalopathies (TSEs), or prion diseases, are progressive neurodegenerative disorders that affect humans and animals. Current treatments for these diseases are ineffective. The misfolding of prion protein is a key factor in the pathogenesis of TSEs, and alterations in protein homeostasis worsen the severity of the disease. Restoring protein homeostasis pathways through pharmacological interventions may help reduce the burden of misfolded prion protein and improve the condition of patients.
FRONTIERS IN NEUROSCIENCE
(2022)
Article
Neurosciences
Giada Lavigna, Antonio Masone, Ihssane Bouybayoune, Ilaria Bertani, Jacopo Lucchetti, Marco Gobbi, Luca Porcu, Stefano Zordan, Mara Rigamonti, Luca Imeri, Elena Restelli, Roberto Chiesa
Summary: Doxycycline shows some therapeutic effects in the FFI mouse model, improving cognitive impairment and motor symptoms associated with sleep dysfunction, but it does not prevent the onset and progression of the disease, nor does it change the amount of aggregated protein or microglial activation.
NEUROBIOLOGY OF DISEASE
(2021)
Article
Medicine, General & Internal
Rachel A. Bender Ignacio, Kara W. Chew, Carlee Moser, Judith S. Currier, Joseph J. Eron, Arzhang Cyrus Javan, Mark J. Giganti, Evgenia Aga, Michael Gibbs, Herve Tchouakam Kouekam, Eva Johnsson, Mark T. Esser, Keila Hoover, Gene Neytman, Matthew Newell, Eric S. Daar, William Fischer, Courtney V. Fletcher, Jonathan Z. Li, Alexander L. Greninger, Robert W. Coombs, Michael D. Hughes, Davey Smith, David Alain Wohl
Summary: These two clinical trials showed that tixagevimab and cilgavimab monoclonal antibodies were safe and effective for treating early COVID-19, but did not change the time to symptom improvement. The treatment outcomes were better for the intramuscular injection.
Article
Neurosciences
Hasier Erana, Carlos M. Diaz-Dominguez, Jorge M. Charco, Enric Vidal, Ezequiel Gonzalez-Miranda, Miguel A. Perez-Castro, Patricia Pineiro, Rafael Lopez-Moreno, Cristina Sampedro-Torres-Quevedo, Leire Fernandez-Veiga, Juan Tasis-Galarza, Nuria L. Lorenzo, Aileen Santini-Santiago, Melisa Lazaro, Sandra Garcia-Martinez, Nuno Goncalves-Anjo, Maitena San-Juan-Ansoleaga, Josu Galarza-Ahumada, Eva Fernandez-Munoz, Samanta Giler, Mikel Valle, Glenn C. Telling, Marivi Geijo, Jesus R. Requena, Joaquin Castilla
Summary: This study presents a method that consistently induces spontaneous misfolding of recombinant PrP into prions within hours, providing unprecedented opportunities to investigate the mechanisms underlying sporadic prion diseases.
ACTA NEUROPATHOLOGICA COMMUNICATIONS
(2023)
Review
Virology
Jiyan Ma, Jingjing Zhang, Runchuan Yan
Summary: The generation of recombinant prions has provided valuable insights into the characteristics and effects of prions. Recombinant prions can exist in various misfolded conformations and have different outcomes when inoculated into wild-type animals. The ability to seed alone is not sufficient to determine prion activity, as authentic prions need to be both heritable and pathogenic. Research on recombinant prions is important for understanding the pathogenesis of prion diseases and the role of misfolded proteins in other neurodegenerative disorders.
Article
Chemistry, Analytical
Thuy Tran, Erik Martinsson, Sergio Vargas, Ingemar Lundstrom, Carl-Fredrik Mandenius, Daniel Aili
Summary: The production of therapeutic monoclonal antibodies is a complex process that requires extensive analytical characterization. Aggregation of antibodies is common and problematic, as it can affect the product quality and immunogenicity. Rapid detection of aggregates is challenging with current analytical techniques.
ANALYTICAL CHEMISTRY
(2022)
Review
Biochemistry & Molecular Biology
Kara M. Gillmann, J. Sebastian Temme, Samantha Marglous, Claire E. Brown, Jeffrey C. Gildersleeve
Summary: Anti-glycan monoclonal antibodies (mAbs) have diverse applications in human health and basic research, including cancer diagnostics and therapeutics. Despite the limited supply of high-quality anti-glycan mAbs, recent advances have been made in targeting cancer- and infectious disease-associated glycans, providing new opportunities for discovery and development of therapeutic antibodies.
CURRENT OPINION IN CHEMICAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Keiji Uchiyama, Hideyuki Hara, Junji Chida, Agriani Dini Pasiana, Morikazu Imamura, Tsuyoshi Mori, Hanae Takatsuki, Ryuichiro Atarashi, Suehiro Sakaguchi
Summary: Advanced DMEM may contain anti-prion compounds that reduce PrPSc levels, with ethanolamine identified as having anti-prion activity. Oral administration of ethanolamine delayed the onset of prion disease in mice, suggesting it could be a new anti-prion compound.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Surbhi Chaudhary, Asmita Dhiman, Anil Patidar, Himanshu Malhotra, Sharmila Talukdar, Rahul Dilawari, Gaurav Kumar Chaubey, Radheshyam Modanwal, Chaaya Iyengar Raje, Manoj Raje
Summary: Studies have shown that the cytoplasmic glyceraldehyde-3-phosphate dehydrogenase (GAPDH) can prevent protein aggregation, maintain proteins in a soluble state, and interact with cyPrP and httQ-103.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2021)
Article
Chemistry, Medicinal
Kun Zhou, HaoFei Hong, Han Lin, Liang Gong, Dan Li, Jie Shi, Zhifang Zhou, Fei Xu, Zhimeng Wu
Summary: The development of mAb-hapten conjugates can enhance effector functions of monoclonal antibodies, leading to improved cancer cell killing activity and significant antitumor efficacy in xenograft models.
JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Review
Geriatrics & Gerontology
Noel A. Jackson, Marcos J. Guerrero-Munoz, Diana L. Castillo-Carranza
Summary: This paper summarizes the novel findings of tau oligomers transmission and propagation via exosomes. Tau oligomers can be transferred from neuron to neuron, propagating through the brain and causing neurodegeneration, playing an important role in early-onset neurodegenerative pathogenesis.
FRONTIERS IN AGING NEUROSCIENCE
(2022)
Meeting Abstract
Biophysics
Mona Habibi, Joerg Rottler, Steven S. Plotkin
BIOPHYSICAL JOURNAL
(2016)
Article
Biochemistry & Molecular Biology
Judith M. Silverman, Ebrima Gibbs, Xubiao Peng, Kris M. Martens, Claudia Balducci, Jing Wang, Masoud Yousefi, Catherine M. Cowan, Guillaume Lamour, Sarah Louadi, Yuxin Ban, Jerome Robert, Sophie Stukas, Gianluigi Forloni, Ging-Yuek R. Hsiung, Steven S. Plotkin, Cheryl L. Wellington, Neil R. Cashman
ACS CHEMICAL NEUROSCIENCE
(2018)
Meeting Abstract
Biophysics
Xubiao Peng, Ebrima Gibbs, Judith M. Silverman, Neil R. Cashman, Steven S. Plotkin
BIOPHYSICAL JOURNAL
(2018)
Article
Biophysics
Mona Habibi, Steven S. Plotkin, Jorg Rottler
BIOPHYSICAL JOURNAL
(2018)
Article
Biochemistry & Molecular Biology
Natalie E. Farrawell, Maddison R. Yerbury, Steven S. Plotkin, Luke McAlary, Justin J. Yerbury
ACS CHEMICAL NEUROSCIENCE
(2019)
Article
Biochemistry & Molecular Biology
John H. Dupuis, Hua Yu, Mona Habibi, Xubiao Peng, Steven S. Plotkin, Shenlin Wang, Chen Song, Rickey Y. Yada
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2018)
Article
Chemistry, Physical
Xubiao Peng, Neil R. Cashman, Steven S. Plotkin
JOURNAL OF PHYSICAL CHEMISTRY B
(2018)
Article
Multidisciplinary Sciences
Ebrima Gibbs, Judith M. Silverman, Beibei Zhao, Xubiao Peng, Jing Wang, Cheryl L. Wellington, Ian R. Mackenzie, Steven S. Plotkin, Johanne M. Kaplan, Neil R. Cashman
SCIENTIFIC REPORTS
(2019)
Review
Clinical Neurology
Luke McAlary, Steven S. Plotkin, Neil R. Cashman
Article
Biochemistry & Molecular Biology
Apar Pataer, Bulent Ozpolat, RuPing Shao, Neil R. Cashman, Steven S. Plotkin, Charles E. Samuel, Steven H. Lin, Nashwa N. Kabil, Jing Wang, Mourad Majidi, Bingliang Fang, Jack A. Roth, Ara A. Vaporciyan, Ignacio I. Wistuba, Mien-Chie Hung, Stephen G. Swisher
Review
Neurosciences
Luke McAlary, Steven S. Plotkin, Justin J. Yerbury, Neil R. Cashman
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2019)
Article
Biochemistry & Molecular Biology
Sabrina Semmler, Myriam Gagne, Pranav Garg, Sarah R. Pickles, Charlotte Baudouin, Emeline Hamon-Keromen, Laurie Destroismaisons, Yousra Khalfallah, Mathilde Chaineau, Elise Caron, Andrew N. Bayne, Jean-Francois Trempe, Neil R. Cashman, Alexandra T. Star, Arsalan S. Haqqani, Thomas M. Durcan, Elizabeth M. Meiering, Janice Robertson, Nathalie Grandvaux, Steven S. Plotkin, Heidi M. McBride, Christine Vande Velde
JOURNAL OF BIOLOGICAL CHEMISTRY
(2020)
Correction
Neurosciences
Luke McAlary, Steven S. Plotkin, Justin J. Yerbury, Neil R. Cashman
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2020)
Article
Biochemistry & Molecular Biology
Mona Habibi, Jorg Rottler, Steven S. Plotkin
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2017)
Meeting Abstract
Biophysics
Mona Habibi, Joerg Rottler, Steven S. Plotkin
BIOPHYSICAL JOURNAL
(2017)