期刊
PROSTATE
卷 72, 期 9, 页码 948-954出版社
WILEY
DOI: 10.1002/pros.21498
关键词
reversion-inducing cysteine-rich protein with Kazal motifs; prostate carcinoma; invasion; MMP
资金
- Deutsche Forschungsgemeinschaft [STE 996/2-1, ER 550/1-1]
- Sonnenfeld-Stiftung
BACKGROUND RECK is a tumor suppressor which inhibits metastasis and angiogenesis. Based on RECK expression in prostate cancer tissue and cell lines, our aim was to investigate functional relevance of RECK for prostate carcinoma. METHODS RECK protein levels were determined by Western blotting in the human prostate cell lines BPH-1, DU-145, LNCaP, PC-3, and in tissue of 12 normal/tumor matches of patients after radical prostatectomy. Functional characteristics of DU-145 cells with stable RECK overexpression included proliferation, invasion, regulation of matrix metalloproteinases MMP-2, MMP-9, and MMP-14 measured by zymography (MMP-2 and -9) or commercially available assays. RESULTS RECK was expressed in cell lines and tissue with a significant decrease in malignant tissue (P?=?0.002). RECK overexpression caused an up to 80% decrease in invasion for DU-145 cells (P?
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