Article
Environmental Sciences
Lakshmi Thangavelu, Royapuram Veeraragavan Geetha, Ezhilarasan Devaraj, Kamal Dua, Dinesh Kumar Chellappan, Sri Renukadevi Balusamy
Summary: The study evaluates the cytotoxic potentials of Acacia catechu ethanolic seed extract in human hepatocellular carcinoma cells, finding that it induces cytotoxicity through increased ROS production, oxidative stress regulation, and apoptosis pathways.
ENVIRONMENTAL TOXICOLOGY
(2022)
Article
Environmental Sciences
Yuanyuan Zhou, Hanyu Liao, Shanshan Yin, Pengqiao Wang, Xiaoqing Ye, Jianyun Zhang
Summary: Organophosphate esters (OPEs) are widely used man-made chemicals detected in various human-related samples. This study evaluated the toxicity and mechanisms of nine commonly detected OPEs on human hepatocellular carcinomas. The OPEs induced apoptosis, oxidative stress, endoplasmic reticulum stress, and NLRP3 inflammasome activation. Different types of OPEs showed varying effects on ROS overproduction, DNA damage, and cytotoxicity.
ENVIRONMENTAL POLLUTION
(2023)
Article
Pharmacology & Pharmacy
V. H. Amstutz, A. Cengo, F. Gehres, D. T. H. M. Sijm, M. F. Vrolijk
Summary: This study investigated the structure-activity relationship between per- and polyfluoroalkyl substances (PFAS) and hepatotoxicity. The results showed that the carbon chain-length and functional head-group of PFAS determine their toxicity, providing the possibility of developing a predictive model for risk assessment.
Article
Biochemistry & Molecular Biology
Raja Rezgui, Ruhi Walia, Jyoti Sharma, Dwinder Sidhu, Khalid Alshagadali, Saumya Ray Chaudhuri, Amir Saeed, Priyankar Dey
Summary: This study demonstrates that the metabolites from Lactobacillus plantarum (LPM), a probiotic bacterium, can protect HepG2 cells against oxidative damage induced by H2O2. The cytoprotective effects of LPM are likely mediated by an Nrf2-dependent mechanism.
Article
Agriculture, Multidisciplinary
Xiaowen Chang, Shan Dong, Wenliang Bai, Yan Di, Ruijuan Gu, Fuguo Liu, Beita Zhao, Yutang Wang, Xuebo Liu
Summary: The methylated metabolites of chicoric acid, M1 and M2, showed better antioxidant capacities than chicoric acid itself, protecting cells from oxidative stress damage through various pathways. This suggests that these metabolites may be responsible for the biological efficacies attributed to chicoric acid.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Agriculture, Multidisciplinary
Bowei Du, Chao Zhang, Guiya Deng, Shuai Zhang, Shiqi Wang, Yuepeng Guan, Yaqin Huang
Summary: This study identified novel antioxidant collagen peptides and investigated their mechanisms of action in blocking Keap1-Nrf2 interactions. The results showed that IDGRPGPIGPA exhibited high scavenging capacity for free radicals, while GPAGPpGPIG improved the resistance of cells to oxidative damage by activating intracellular antioxidant enzymes and glutathione production.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2023)
Article
Food Science & Technology
Yang Zhao, Sainan Liu, Zhili Sheng, Xue Li, Yanan Chang, Weichang Dai, Sui Kiat Chang, Junmei Liu, Yuchun Yang
Summary: The study indicated that pinolenic acid (PNA) can improve the survival rate of HepG2 cells induced by H2O2, reduce the accumulation of intracellular ROS, and decrease the level of intracellular malondialdehyde (MDA). The mechanism of PNA regulating oxidative stress involves the upregulation of antioxidant enzymes through the Keap1/Nrf2 transcriptional pathway.
FOOD SCIENCE & NUTRITION
(2021)
Article
Biochemistry & Molecular Biology
Juan Luis Penas-Fuentes, Eva Siles, Eva E. Rufino-Palomares, Amalia Perez-Jimenez, Fernando J. Reyes-Zurita, Jose A. Lupianez, Carlos Fuentes-Almagro, Juan Peragon-Sanchez
Summary: This study characterized the cytotoxic effects of erythrodiol (EO) on human hepatocarcinoma cells. EO decreased cell viability and antioxidant defense systems, impaired important metabolic processes and nuclear transport, but induced AMP biosynthesis and cell cycle G2/M phase transition.
Article
Fisheries
Yanan Li, Zongfu Chen, Yayuan Xiao, Feng Gao, Fanbin Zhan, Zhijie Lu, Zhenpeng Huang, Xuefeng Wei, Fengping Su, Fei Shi, Li Lin, Zhendong Qin
Summary: Respiratory burst is a process of producing reactive oxygen species in response to pathogens, but excessive production can be harmful. The Keap1-Nrf2-ARE pathway plays a role in alleviating oxidative stress and maintaining cellular homeostasis. The role of Keap1 during bacterial infection in fish is not well understood.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Plant Sciences
Thabani Sibiya, Terisha Ghazi, Jivanka Mohan, Savania Nagiah, Anil A. Chuturgoon
Summary: Spirulina platensis has shown high potency in the treatment of oxidative stress, diabetes, immune disorder, inflammatory stress, and bacterial and viral-related diseases. This study found that Spirulina platensis can protect against ARV-induced oxidative stress in HepG2 cells. The activation of the antioxidant response by Spirulina platensis can mitigate the adverse drug reactions of HAART.
Article
Biochemistry & Molecular Biology
Marialuce Giorgini, Mercedes Taroncher, Josefa Tolosa, Maria-Jose Ruiz, Yelko Rodriguez-Carrasco
Summary: The decreasing bee population caused by Varroa destructor parasite poses a threat to the production of bee-products, which are in high demand. Beekeepers commonly use the pesticide amitraz to minimize the negative effects of this parasite. This study aims to determine the toxic effects of amitraz and its metabolites in HepG2 cells, analyze their presence in honey samples, investigate their stability under heat treatments in the honey industry, and explore their relationship with the production of 5-hydroxymethylfurfural (HMF). The results show that amitraz and its metabolites have significant cytotoxic and oxidative stress effects. Residues of amitraz and its main metabolite, 2,4-Dimethylaniline (2,4-DMA), were found in analyzed honey samples. Furthermore, both amitraz and HMF showed instability even under moderate heat treatments, with HMF concentration positively correlated with the severity of heat treatment.
Article
Oncology
Fatih Altintas, Melek Tunc-Ata, Mucahit Secme, Vural Kucukatay
Summary: The study evaluated the cytotoxic, genotoxic, apoptotic, oxidant and antioxidant effects of thymol on HepG2 cell line. Thymol showed cytotoxic and genotoxic effects on HepG2 cells, while having no lethal effect on healthy cells. It also decreased oxidative stress and induced apoptosis in HepG2 cells, without altering the gene expressions associated with apoptosis. The results suggest the potential of thymol in the treatment of liver cancer.
Article
Biochemistry & Molecular Biology
Hyeongyeong Kim, Hyung Joo Suh, Ki-Bae Hong, Eun-Jin Jung, Yejin Ahn
Summary: The protective effects of a mixture of cysteine and glutathione against ethanol-induced hangover and liver damage were investigated. The mixture improved alcohol-induced behavior and enhanced ethanol-metabolizing enzyme activity. It also reduced the levels of serum alcohol and acetaldehyde, and regulated the expression of antioxidant enzymes through the Nrf2 pathway.
Article
Biochemistry & Molecular Biology
Yidong Xiao, Xiao Zhang, Qilin Huang
Summary: This study investigated the protective effects of Cordyceps sinensis exopolysaccharide-selenium nanoparticles (EPS-SeNPs) against oxidative stress in human hepatoma cells. The results showed that EPS-SeNPs promoted cell proliferation, restored cell morphology, and reduced LDH levels. Additionally, EPS-SeNPs inhibited ROS production and increased antioxidant levels. The protective effects of EPS-SeNPs were attributed to their smaller particle size.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Plant Sciences
Thabani Sibiya, Terisha Ghazi, Jivanka Mohan, Savania Nagiah, Anil A. Chuturgoon
Summary: This study investigates the mechanisms of microRNA expression in HepG2 cells in the presence of HAART and SP. The results show that miR-146a and miR-155 levels increased in SP-treated cells, while only miR-146a increased in HAART-SP treatment and miR-155 decreased. Furthermore, Cox-1 expression increased in HAART-SP-treated cells, while Cox-2 and mRNA expression decreased. These findings suggest that SP potentially controls inflammation by regulating microRNA expressions and exhibit a positive synergistic effect with HAART.
Article
Biochemistry & Molecular Biology
Mattia Di Nunzio, Cecilia Loffi, Elena Chiarello, Luca Dellafiora, Gianfranco Picone, Giorgia Antonelli, Clarissa Di Gregorio, Francesco Capozzi, Tullia Tedeschi, Gianni Galaverna, Alessandra Bordoni
Summary: Reducing the salt content in Parmigiano Reggiano cheese by shortening brine soaking time can effectively maintain sensory and nutritional properties while decreasing the risk of diet-related diseases.
Article
Nutrition & Dietetics
Elena Chiarello, Mattia Di Nunzio, Gianfranco Picone, Giorgia Antonelli, Francesco Capozzi, Alessandra Bordoni
Summary: This study examined the metabolism and absorption of glucose, fructose, and sucrose in enterocytes. The results suggest that fructose is taken up and catabolized more quickly than glucose, and the metabolism of these sugars differs in the cells. Further research is needed to confirm these findings and to explore specific transporters and enzymes involved in sugar metabolism.
Article
Food Science & Technology
George Bazar, Hajnalka Hingyi, Eva Csavajda, Csaba Palko, Haruna Gado Yakubu, Carlos Pineda-Vadillo, Didier Dupont, Francesco Capozzi, Alessandra Bordoni, Tamas Toth
Summary: This study used electronic sensor array technology to test pancakes enriched with docosahexaenoic acid and anthocyanins. The results showed a strong relation between the number of days that samples were stored and the odor describing sensor signals of enriched samples. The electronic nose technology proved to be applicable in the characterization of bioactive-enriched foods and useful in monitoring odor alterations during storage.
FOOD ANALYTICAL METHODS
(2022)
Article
Biotechnology & Applied Microbiology
Arianna Ricci, Jaime A. Arboleda Mejia, Andrea Versari, Elena Chiarello, Alessandra Bordoni, Giuseppina P. Parpinello
Summary: This study focused on the encapsulation of bioactive polyphenolic compounds from Cabernet Sauvignon wine lees using nanofiltration and spray drying techniques. The optimized microcapsules exhibited high stability and durability, and showed potential for gastrointestinal absorption.
FOOD AND BIOPRODUCTS PROCESSING
(2022)
Article
Biochemistry & Molecular Biology
Carlo Mengucci, Lorenzo Nissen, Gianfranco Picone, Corinne Malpuech-Brugere, Caroline Orfila, Luigi Ricciardiello, Alessandra Bordoni, Francesco Capozzi, Andrea Gianotti
Summary: This study proposes the development of an explorative tool based on correlation networks to integrate omics data and investigate the physiological effects of nutritional interventions. By combining data from microbiota, metabolome, and volatilome, this approach can reveal the complex relationship between nutrition and health.
Article
Biochemistry & Molecular Biology
Mattia Di Nunzio, Cecilia Loffi, Serena Montalbano, Elena Chiarello, Luca Dellafiora, Gianfranco Picone, Giorgia Antonelli, Tullia Tedeschi, Annamaria Buschini, Francesco Capozzi, Gianni Galaverna, Alessandra Bordoni
Summary: Replacing synthetic nitrates/nitrites with natural additives can be a promising strategy to develop clean label salamis without negatively affecting their nutritional value.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Stefania Iametti, Francesco Bonomi, Mattia Di Nunzio
Summary: Recent evidence suggests that chronic consumption of large amounts of fructose is associated with several non-communicable diseases. Plant polyphenols have the potential to inhibit fructose uptake and transport, making them an important target for studying the effects on fructose metabolism. However, the lack of standardized conditions in current studies prevents definitive conclusions. Future research should focus on standardized conditions to obtain comparable results.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Peter Riber Johnsen, Cecilia Pinna, Luce Mattio, Mathilde Bech Strube, Mattia Di Nunzio, Stefania Iametti, Sabrina Dallavalle, Andrea Pinto, Hanne Frokiaer
Summary: This study compares the effects of seven different structural types of stilbenoids on the modulation of cytokine production and antioxidant response. Monomeric compounds showed dose-dependent inhibition of cytokine production induced by E. coli, with resveratrol and piceatannol also inhibiting IL-10 production. All monomers, except trimethoxy-resveratrol, inhibited cytokine production induced by L. acidophilus. However, the dimer dehydro-delta-viniferin remarkably enhanced IL-12 production induced by L. acidophilus.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Nutrition & Dietetics
Alessandra Bordoni
Summary: As we deal with climate change and scarcity of natural resources, the challenge is to provide sufficient, nutritious, safe, and affordable food for a growing global population without harming the environment. The water footprint (WF) is an important indicator of the environmental impact of diets, and this study evaluates the WF of the food patterns suggested by the Italian Food Based Dietary Guidelines for the first time. The results show that the suggested Italian dietary patterns have a low WF, but consumer choices and providing information to farmers and producers can further reduce water usage.
Review
Biochemistry & Molecular Biology
Sara Margherita Borgonovi, Stefania Iametti, Mattia Di Nunzio
Summary: Docosahexaenoic acid (DHA) is a polyunsaturated fatty acid that shows potential in controlling cellular antioxidant defenses through Nrf2 activation. Studies in cell cultures and animals indicate positive effects of DHA on cellular antioxidant enzymes. However, variations in experimental conditions might account for the observed differences among studies.
Article
Food Science & Technology
Sara Margherita Borgonovi, Elena Chiarello, Federica Pasini, Gianfranco Picone, Silvia Marzocchi, Francesco Capozzi, Alessandra Bordoni, Alberto Barbiroli, Alessandra Marti, Stefania Iametti, Mattia Di Nunzio
Summary: Buckwheat is recognized as a good source of nutrients and is receiving increasing attention as a potential functional food. This study found that sprouting buckwheat for 48 and 72 hours increased the content of peptides and free-phenolic compounds, improved antioxidant activity, reduced anti-nutritional components, and improved the overall nutritional characteristics. These results confirm the potential of sprouted buckwheat as a high-quality ingredient for innovative products.
Article
Food Science & Technology
Giorgia Antonelli, Elena Chiarello, Gianfranco Picone, Silvia Tappi, Giulia Baldi, Mattia Di Nunzio, Eleni Mente, Stelios Karapanagiotis, Phelly Vasilaki, Massimiliano Petracci, Pietro Rocculi, Alessandra Bordoni, Francesco Capozzi
Summary: Fish is an essential part of the human diet, with an increasing proportion expected to come from aquaculture production in the future. Sustainable growth in aquaculture requires the use of new raw materials while maintaining the nutritional value of fish meat. The study evaluated the impact of a newly formulated feed and preservation treatments on the composition and digestibility of sea bass fillets, finding that the formulated feed maintained good nutritional value and pulsed electric field (PEF) showed promise as a preservation technology.
