期刊
PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS
卷 81, 期 5-6, 页码 417-423出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.plefa.2009.07.003
关键词
Flaxseed; Ultrasound; Sonication; Lipid extraction; Fatty acids; High throughput
资金
- Ontario Centres of Excellence Interact
- Certo Labs, Inc. Infrastructure
- Canada Foundation of Innovation
- Ontario Research Fund
Ultrasound may reduce lipid extraction times and increase analysis throughput of food materials. Ground flaxseed (25 mg aliquots) were extracted in quadruplicate in 2:1 (v:v) chloroform: methanol, 3:2 hexane:isopropanol, 1:1 diethyl: petroleum ether or hexane with exposure to sonication at low frequencies of 20 kHz with a 600 W ultrasonic processor. Power was automatically varied to maintain constant amplitudes of 20%, 60% and 100% of 240 mu m for sonication exposures for 5, 10 and 20 min, respectively. Total lipid dry weights and quantitative and qualitative fatty acids were determined. Results were compared to a standard 24-h, Folch-based, 2:1 chloroform: methanol extraction. Longer time exposures and higher sonication amplitudes were associated with increases in lipid recoveries. In particular, ultrasound-assisted extraction in 3:2 hexane:isopropanol for only 10 min resulted in lipid and fatty acid recoveries similar to the 24-h standard method. Comprehensive testing on a variety of sample matrices and food products is required, but lipid extraction by ultrasound has potential to reduce sample processing time. (C) 2009 Elsevier Ltd. All rights reserved.
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