Article
Biology
Jacinda Chen, Amaury Cazenave-Gassiot, Yimeng Xu, Paola Piroli, Robert Hwang Jr, Laura DeFreitas, Robin Barry Chan, Gilbert Di Paolo, Renu Nandakumar, Markus R. Wenk, Catherine Marquer
Summary: This study identifies lysosomal phospholipase A2 (LPLA2) as the enzyme responsible for converting phosphatidylglycerol (PG) into lysophosphatidylglycerol (LPG) in the BMP biosynthetic pathway. Modulating LPLA2 levels affects cholesterol levels and late endosome/lysosome morphology.
COMMUNICATIONS BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Oliver Bogojevic, Yan Zhang, Christian Daugaard Wolff, Jens Vinge Nygaard, Lars Wiking, Carl Arevang, Zheng Guo
Summary: A sustainable and green synthesis of Hemi-BMPs and BDPs, uncommon naturally occurring phospholipids, was achieved via phospholipase D (PLD) mediated transphosphatidylation. The enzyme from Streptomyces sp. exhibited excellent substrate promiscuity for phospholipids from different sources and alcohol donors with diverse structures, leading to high yields in a short reaction time. The reaction rate was found to be dependent on the size, shape, and regiolocation of fatty acyls on the glycerol backbone, especially for bulky diacylglycerol regio-isomers.
Article
Multidisciplinary Sciences
Uche N. Medoh, Andy Hims, Julie Y. Chen, Ali Ghoochani, Kwamina Nyame, Wentao Dong, Monther Abu-Remaileh
Summary: Lysosomes rely on BMP to stimulate lipid catabolism, cholesterol homeostasis, and lysosomal function. CLN5 is identified as the elusive BMP synthase, and it mediates synthesis through a base exchange reaction between two LPG molecules.
Article
Chemistry, Multidisciplinary
Oliver Bogojevic, Yan Zhang, Christian Daugaard Wolff, Jens Vinge Nygaard, Lars Wiking, Carl Arevang, Zheng Guo
Summary: Progress in developing synthetic pathways for novel and complex phospholipid species, such as Hemi-BMPs and BDPs, is crucial for expanding the availability and understanding of rare phospholipids. These phospholipids have gained attention for their applications in COVID-19 vaccines, lysosomal storage disorders, and cancers. The presented work demonstrates a successful system for producing structurally diverse Hemi-BMP and BDP analogues, contributing to the knowledge and potential breakthroughs in this field.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2023)
Article
Microbiology
Ruben M. Markosyan, Mariana Marin, You Zhang, Fredric S. Cohen, Gregory B. Melikyan
Summary: This study investigated the cell-cell fusion induced by the Old World Lassa virus GPC complex and found that viral entry into host cells is mainly regulated by the human LAMP1 receptor and a specific anionic lipid, bis(monoacylglycero)phosphate. The findings suggest that arenaviruses have evolved to utilize bis(monoacylglycero)phosphate to enter cells from late endosomes.
Article
Biochemistry & Molecular Biology
Francoise Hullin-Matsuda, Pascal Colosetti, Maxence Rabia, Celine Luquain-Costaz, Isabelle Delton
Summary: Extracellular vesicles (EVs) are various membrane-bound vesicles secreted by cells and found in biological fluids, which have gained attention as mediators of cell-cell communication and for their roles in physiological processes. They are also considered as disease biomarkers and drug delivery systems. However, it is challenging to link a biological function to a specific EV population due to limitations in isolation methods. In this review, the composition and role of EV lipids, which have received less attention compared to proteins and nucleic acids, are summarized. The utility of a specific endolysosomal lipid marker, bis(monoacylglycero)phosphate (BMP), in indicating the endosomal origin of small EVs, known as exosomes, is discussed.
Review
Biochemistry & Molecular Biology
Mai K. L. Nguyen, Jaimy Jose, Mohamed Wahba, Marc Bernaus-Esque, Andrew J. Hoy, Carlos Enrich, Carles Rentero, Thomas Grewal
Summary: Cancer cells undergo metabolic adaptions to meet their increased bioenergetic needs, including an increased demand for cholesterol. They uptake cholesterol from low-density lipoproteins (LDL) and distribute it from late endosomes/lysosomes (LE/Lys) to other organelles to promote cancer growth and spread. Understanding the regulation of cholesterol transporters and related proteins in LE/Lys is important for developing treatment strategies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Physical
Steinar Mannsverk, Ana M. Villamil Giraldo, Peter M. Kasson
Summary: The phospholipid bis(monoacylglycero)phosphate (BMP) is enriched in late endosomal and endolysosomal membranes and plays a role in membrane deformation and generation of intralumenal vesicles within late endosomes. This study found that BMP enhances full fusion efficiency of influenza virus by affecting fusion pore formation and genome exposure, suggesting that BMP may serve as a general cofactor for endosomal entry of enveloped viruses.
JOURNAL OF PHYSICAL CHEMISTRY B
(2022)
Article
Microbiology
Simon Czolkoss, Pia Borgert, Tessa Poppenga, Georg Hoelzl, Meriyem Aktas, Franz Narberhaus
Summary: Some soil-dwelling bacteria have the ability to metabolize exogenous lyso-phospholipids and convert them into non-canonical lipid bis(monoacylglycero)phosphate (BMP), indicating a remarkable adaptive response of bacterial membranes to environmental changes.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Hematology
Do Huu Chi, Tomoaki Kahyo, Ariful Islam, Md Mahmudul Hasan, A. S. M. Waliullah, Md Al Mamun, Madoka Nakajima, Takenori Ikoma, Keitaro Akita, Yuichiro Maekawa, Tomohito Sato, Mitsutoshi Setou
Summary: Supplementation of docosahexaenoic acid or eicosapentaenoic acid to the Western diet resulted in higher levels of NAD(+), FAD(+), and their metabolites in the aortic arch.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Amitha Muraleedharan, Benoit Vanderperre
Summary: Parkinson's disease is the second most common neurodegenerative disorder after Alzheimer's disease, and its prevalence increases with age. The endolysosomal system plays a major role in the pathogenesis of PD, and studying the genes and proteins involved in this system can lead to potential therapeutic interventions.
JOURNAL OF MOLECULAR BIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Mariona Jove, Natalia Mota-Martorell, Elia Obis, Joaquim Sol, Meritxell Martin-Gari, Isidre Ferrer, Manuel Portero-Otin, Reinald Pamplona
Summary: The human brain is highly susceptible to oxidative stress due to its characteristics, but neurons have evolved various adaptive mechanisms to combat oxidative stress and preserve their functionality.
Article
Chemistry, Medicinal
Yumei Feng, Yuanmin Zhu, Zhendong Bao, Bohan Wang, Tingting Liu, Huihui Wang, Tianyi Yu, Ying Yang, Longjiang Yu
Summary: This study demonstrated that overexpression of endogenous glucose-6-phosphate dehydrogenase (G6PD) gene in Schizochytrium sp. H016 increased the availability of NADPH, resulting in higher yield of docosahexaenoic acid (DHA) and lipid accumulation. These findings uncovered the beneficial effects of NADPH on PUFA synthesis in Schizochytrium sp. H016 and provided a promising regulatory strategy for large-scale DHA production.
Article
Chemistry, Applied
Ryota Hosomi, Anna Matsudo, Koki Sugimoto, Takaki Shimono, Seiji Kanda, Toshimasa Nishiyama, Munehiro Yoshida, Kenji Fukunaga
Summary: This study evaluated the effects of dietary EPA-ethyl ester (EE) and DHA-EE on steroid metabolism, gut microbiota, and bacterial metabolites in Wistar rats. The results showed that rats fed with DHA had lower microbiota diversity indices and significant differences in the relative abundance of microbiota at the genus level compared to rats fed with EPA or SOY diets. Therefore, it was concluded that EPA and DHA have different effects on the diversity and composition of gut microbiota under the experimental conditions.
JOURNAL OF OLEO SCIENCE
(2021)
Article
Biology
Seii Ohka, Soon Hao Tan, Eri Ishiyama, Katsutoshi Ogasawara, Tomohito Hanasaka, Kinji Ishida, Kyoji Hagiwara, Chia-Chyi Liu, Pele Choi-Sing Chong, Ken-ichi Hanaki, Giampietro Schiavo
Summary: Recent research has shown that EV71, a causative agent of hand-foot-and-mouth disease, undergoes efficient uncoating in late endosomes containing hSCARB2, M6PR, RAB9, bis(monoacylglycero)phosphate, and LAMP2. The autophagic pathway does not play a critical role in EV71 uncoating, and cation-dependent M6PR is likely to be important for this process.
Review
Biochemistry & Molecular Biology
Akiko Yamaji-Hasegawa, Francoise Hullin-Matsuda, Peter Greimel, Toshihide Kobayashi
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2016)
Review
Biochemistry & Molecular Biology
Francoise Hullin-Matsuda, Asami Makino, Motohide Murate, Toshihide Kobayashi
Article
Biochemistry & Molecular Biology
Sathvika Venugopal, Daniel Benjamin Martinez-Arguelles, Seimia Chebbi, Francoise Hullin-Matsuda, Toshihide Kobayashi, Vassilios Papadopoulos
JOURNAL OF BIOLOGICAL CHEMISTRY
(2016)
Article
Cell Biology
Asami Makino, Francoise Hullin-Matsuda, Motohide Murate, Mitsuhiro Abe, Nario Tomishige, Mitsunori Fukuda, Shizuya Yamashita, Toyoshi Fujimoto, Hubert Vidal, Michel Lagarde, Isabelle Delton, Toshihide Kobayashi
MOLECULAR BIOLOGY OF THE CELL
(2016)
Article
Biochemistry & Molecular Biology
Asami Makino, Mitsuhiro Abe, Reiko Ishitsuka, Motohide Murate, Takuma Kishimoto, Shota Sakai, Francoise Hullin-Matsuda, Yukiko Shimada, Takehiko Inaba, Hideyuki Miyatake, Hideko Tanaka, Atsushi Kurahashi, Chan-Gi Pack, Rinshi S. Kasai, Shuku Kubo, Nicole L. Schieber, Naoshi Dohmae, Naoya Tochio, Kyoji Hagiwara, Yutaka Sasaki, Yoko Aida, Fumihiro Fujimori, Takanori Kigawa, Kozo Nishibori, Robert G. Parton, Akihiro Kusumi, Yasushi Sako, Gregor Anderluh, Makoto Yamashita, Tetsuyuki Kobayashi, Peter Greimel, Toshihide Kobayashi
Article
Biochemistry & Molecular Biology
Yinan Chen, Maud Arnal-Levron, Francoise Hullin-Matsuda, Carole Knibbe, Philippe Moulin, Celine Luquain-Costaz, Isabelle Delton
Review
Cell Biology
Neval Yilmaz, Akiko Yamaji-Hasegawa, Francoise Hullin-Matsuda, Toshihide Kobayashi
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2018)
Review
Biochemistry & Molecular Biology
Malcolm Anastasius, Celine Luquain-Costaz, Maaike Kockx, Wendy Jessup, Leonard Kritharides
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2018)
Article
Biochemistry & Molecular Biology
Francoise Hullin-Matsuda, Motohide Murate, Toshihide Kobayashi
CHEMISTRY AND PHYSICS OF LIPIDS
(2018)
Article
Biochemistry & Molecular Biology
Maud Arnal-Levron, Yinan Chen, Peter Greimel, Federica Calevro, Karen Gaget, Fabien Riols, Aurelie Batut, Justine Bertrand-Michel, Francoise Hullin-Matsuda, Vesa M. Olkkonen, Isabelle Delton, Celine Luquain-Costaz
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2019)
Article
Biochemistry & Molecular Biology
Maxence Rabia, Valentin Leuzy, Christophe Soulage, Annie Durand, Baptiste Fourmaux, Elisabeth Errazuriz-Cerda, Rene Koffel, Annette Draeger, Pascal Colosetti, Audrey Jalabert, Mathilde Di Filippo, Audrey Villard-Garon, Cyrille Bergerot, Celine Luquain-Costaz, Philippe Moulin, Sophie Rome, Isabelle Delton, Francoise Hullin-Matsuda
Article
Biochemistry & Molecular Biology
Celine Luquain-Costaz, Maxence Rabia, Francoise Hullin-Matsuda, Isabelle Delton
Article
Biochemistry & Molecular Biology
Marine Leroux, Hana Bouazizi-Ben Messaoud, Celine Luquain-Costaz, Lars P. Jordheim, Pauline Le Faouder, Marie-Paule Gustin, Karim Aoun, Philippe Lawton, Samira Azzouz-Maache, Isabelle Delton
Summary: Leishmania parasites cause visceral or cutaneous leishmaniasis in humans and canine leishmaniosis. The fatty acid composition (FA) of lipids in Leishmania donovani changes during the differentiation of promastigotes to amastigotes. Supplementation of arachidonic acid (AA) or docosahexaenoic acid (DHA) in Leishmania infantum strains enhances the proportion of these FA in lipids and the production of FA-derived oxygenated metabolites. AA-supplemented promastigotes show increased infectivity towards macrophages, while DHA supplementation only enhances infectivity in the MON-24 strain. The accumulation of FA derivatives in parasites under high AA or DHA environment may modulate parasite infectivity towards host macrophages.
Article
Hematology
Celine Luquain-Costaz, Maaike Kockx, Malcolm Anastasius, Vincent Chow, Anatol Kontush, Wendy Jessup, Leonard Kritharides
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2020)