期刊
PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS
卷 81, 期 1, 页码 73-78出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.plefa.2009.04.009
关键词
Human platelet aggregation; Spice active principles; Eugenol; Capsaicin; Aspirin; Arachidonic acid; Collagen; ADP; Thromboxane B-2
资金
- Department of Science and Technology, New Delhi
- Council for scientific and Industrial Research (CSIR), New Delhi, India
Spice active principles are reported to have anti-diabetic, anti-hypercholesterolemic, antilithogenic, anti-inflammatory, anti-microbial and anti-cancer properties. In our previous report we have shown that spices and their active principles inhibit 5-lipoxygenase and also formation of leukotriene C4. In this study, we report the modulatory effect of spice active principles viz., eugenol, capsaicin, piperine, quercetin, curcumin, cinnamaldehyde and allyl sulphide on in vitro human platelet aggregation. We have demonstrated that spice active principles inhibit platelet aggregation induced by different agonists, namely ADP (50 mu M), collagen (500 mu g/ml), arachidonic acid (AA) (1.0mM) and calcium ionophore A-23187 (20 mu M). Spice active principles showed preferential inhibition of arachidonic acid-induced platelet aggregation compared to other agonists. Among the spice active principles tested, eugenol and capsaicin are found to be most potent inhibitors of AA-induced platelet aggregation with IC50 Values of 0.5 and 14.6 mu M, respectively. The order of potency of spice principles in inhibiting AA-induced platelet aggregation is eugenol > capsaicin > curcumin > cinnamaldehyde > piperine > ally] sulphide>quercetin. Eugenol is found to be 29-fold more potent than aspirin in inhibiting AA-induced human platelet aggregation. Eugenol and capsaicin inhibited thromboxane B2 (TXB2) formation in platelets in a dose-dependent manner challenged with AA apparently by the inhibition of the cyclooxygenase (COX-1). Eugenol-mediated inhibition of platelet aggregation is further confirmed by dose-dependent decrease in malondialdehyde (MDA) in platelets. Further, eugenol and capsaicin inhibited platelet aggregation induced by agonists-collagen, ADP and calcium ionophore but to a lesser degree compared to AA. These results clearly suggest that spice principles have beneficial effects in modulating human platelet aggregation. (C) 2009 Elsevier Ltd. All rights reserved.
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