期刊
PROGRESS IN NATURAL SCIENCE-MATERIALS INTERNATIONAL
卷 19, 期 11, 页码 1529-1536出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.pnsc.2009.04.010
关键词
Pyrene; Hydrophobicity; Drug carrier; Fluorescence; Amphiphilic peptide
资金
- Ministry of Education of China to Sichuan University
- Analytical and Testing Center of Sichuan University, Chengdu, China
The amphiphilic peptide is becoming attractive as a potential drug carrier to improve the dissolvability of hydrophobic drugs in an aqueous system; thus, facilitating drug uptake by target cells. Here, we report a novel designed amphiphilic peptide, Ac-RADAGAGA-RADAGAGA-NH2, which was able to stabilize pyrene, a hydrophobic model drug we chose to study in aqueous solution. This designed peptide formed a colloidal suspension by encapsulating pyrene inside the peptide-pyrene complex. Egg phosphatidylcholine (EPC) vesicles were used to mimic cell bilayer membranes. We found that pyrene was released from the peptide coating into the EPC vesicles by mixing the colloidal suspension with EPC vesicles, which was followed by steady. fluorescence spectra as a function of time. A calibration curve for the amount of pyrene released into the EPC vesicles at a given time was used to determine the. final concentration of pyrene released into the lipid vesicles from the peptide-pyrene complex. The release rate of the peptide-pyrene complex was calculated to quantify the transfer of pyrene into EPC vesicles. (C) 2009 National Natural Science Foundation of China and Chinese Academy of Sciences. Published by Elsevier Limited and Science in China Press. All rights reserved.
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