Review
Endocrinology & Metabolism
Stanko S. S. Stojilkovic, Tamas Balla
Summary: Plasma membrane and organelle membranes contain phosphoinositides that play important roles in cellular signaling and exocytosis. Three phosphoinositides, PI4P, PI(4,5)P2, and PI(3,4,5)P3, are particularly involved in hormone-producing pituitary cells. PI(4,5)P2 controls various pituitary cell functions, while also serving as a substrate for PI3-kinases to generate intracellular messengers PI(3,4,5)P3 and PI(3,4)P2. PI(4,5)P2 affects pituitary hormone release through ion channel gating, calcium signaling, and the exocytic pathway. PI4P is not only a precursor of PI(4,5)P2 but also a signaling molecule that regulates hormone secretion independently of PI(4,5)P2 in various cell types, including pituitary cells.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Biology
Sarah A. A. Hofbrucker-MacKenzie, Eric Seemann, Martin Westermann, Britta Qualmann, Michael M. M. Kessels
Summary: Using advanced imaging techniques, this study visualizes and quantitatively determines the changes and distribution of PIP2 in dendritic spines during LTD induction. It reveals distinct phases of PIP2 signals and dissects the molecular mechanisms underlying the observed PIP2 dynamics.
COMMUNICATIONS BIOLOGY
(2023)
Article
Microbiology
Paul-Christian Burda, Abhinay Ramaprasad, Sabrina Bielfeld, Emma Pietsch, Anna Woitalla, Christoph Soehnchen, Mehar Nihal Singh, Jan Strauss, Aaron Sait, Lucy M. Collinson, Dominik Schwudke, Michael J. Blackman, Tim-Wolf Gilberger
Summary: The clinical symptoms of malaria arise due to repeated rounds of replication of Plasmodium parasites within red blood cells (RBCs). In this study, the essentiality of 19 putative phospholipase enzymes expressed by the human malaria parasite Plasmodium falciparum during its replication within RBCs is investigated. It is found that there is a high level of functional redundancy among these enzymes, but the phosphoinositide-specific phospholipase C has an essential role in parasite development and cleavage of phosphatidylinositol bisphosphate.
Article
Physiology
Lizbeth de la Cruz, Christopher Kushmerick, Jane M. Sullivan, Martin Kruse, Oscar Vivas
Summary: This study investigates the metabolism of phosphoinositides in different cell types and finds that central neurons have a larger PtdIns(4)P pool and synthesize PtdIns(4,5)P-2 at a faster rate. The study highlights the cell-type specific mechanisms involved in maintaining PtdIns(4,5)P-2 levels.
JOURNAL OF GENERAL PHYSIOLOGY
(2022)
Article
Cell Biology
Archna Ravi, Lavinia Palamiuc, Brooke M. Emerling
Summary: While organelles are individual compartments with specialized functions, inter-organelle communication is essential for cellular homeostasis. This communication is mediated by phospholipids on organelle membranes, particularly phosphoinositides like phosphatidylinositol 4,5-bisphosphate (PI-4,5-P-2). This review focuses on the role of non-canonical Type II PIPKs in generating PI-4,5-P-2 and their impact on organelle crosstalk, cellular health, and human diseases.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Kyungreem Han, Soon Ho Kim, Richard M. Venable, Richard W. Pastor
Summary: This study reveals the atomic-level features of phosphatidylinositol 4,5-bisphosphate (PIP2) clustering in asymmetric bilayers through atomistic simulations and network-theoretic analysis. The specific cation effects, calcium and monovalent cations, are found to determine the design principles of PIP2 clustering.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Cell Biology
Alex G. Batrouni, Nirmalya Bag, Henry T. Phan, Barbara A. Baird, Jeremy M. Baskin
Summary: Phosphatidylinositol 4-kinase III alpha (PI4KIII alpha) is responsible for generating phosphatidylinositol (4)-phosphate at the plasma membrane, and the differential palmitoylation patterns of its anchor protein EFR3B control its distribution and phosphoinositide homeostasis.
JOURNAL OF CELL SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Daniel Barth, Andreas Lueckhoff, Frank J. P. Kuehn
Summary: Recent studies have found that PIP2 plays a crucial role in activating TRPM2 channels, with human TRPM2 being the least sensitive to PIP2 among different species variants. This suggests that changes in intramembranous PIP2 content can regulate the activity of TRPM2 channels in a species-specific manner.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biology
Dino Luethi, Julian Maier, Deborah Rudin, Daniel Szollosi, Thomas J. F. Angenoorth, Stevan Stankovic, Matthias Schittmayer, Isabella Burger, Jae-Won Yang, Kathrin Jaentsch, Marion Holy, Anand Kant Das, Mario Brameshuber, Gisela Andrea Camacho-Hernandez, Andrea Casiraghi, Amy Hauck Newman, Oliver Kudlacek, Ruth Birner-Gruenberger, Thomas Stockner, Gerhard J. Schutz, Harald H. Sitte
Summary: This study investigates the subunit stoichiometry and function of the plasmalemmal norepinephrine transporter (NET), showing the direct regulation of NET organization and function by phosphatidylinositol 4,5-bisphosphate (PIP2).
COMMUNICATIONS BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Maximilian M. Gass, Sarah Borkowsky, Marie-Luise Lotz, Rebecca Siwek, Rita Schroeter, Pavel Nedvetsky, Stefan Luschnig, Astrid Rohlmann, Markus Missler, Michael P. Krahn
Summary: Drosophila nephrocytes, similar to mammalian podocytes, exhibit characteristics of epithelial cells. Research has shown that phospholipid PI(4,5)P2 plays a crucial role in slit diaphragm formation, while PI(3,4,5)P3 has minimal impact on nephrocyte function.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Biology
Alexis Lebecq, Mehdi Doumane, Aurelie Fangain, Vincent Bayle, Jia Xuan Leong, Frederique Rozier, Maria Del Marques-Bueno, Laia Armengot, Romain Boisseau, Mathilde Laetitia Simon, Mirita Franz-Wachtel, Boris Macek, Suayib Ustun, Yvon Jaillais, Marie-Cecile Caillaud
Summary: Membrane lipids, especially phosphoinositides, are differentially enriched within the eukaryotic endomembrane system. In this study, the authors characterized the functional role of a phosphoinositide phosphatase, SAC9, in Arabidopsis. They found that the depletion of SAC9 led to misplaced accumulation of PI(4,5)P-2 on cortical intracellular compartments, impacting endocytic trafficking.
Article
Multidisciplinary Sciences
Kaori Kanemaru, Makoto Shimozawa, Manabu Kitamata, Rikuto Furuishi, Hinako Kayano, Yui Sukawa, Yuuki Chiba, Takatsugu Fukuyama, Junya Hasegawa, Hiroki Nakanishi, Takuma Kishimoto, Kazuya Tsujita, Kazuma Tanaka, Toshiki Itoh, Junko Sasaki, Takehiko Sasaki, Kiyoko Fukami, Yoshikazu Nakamura
Summary: This study reveals the critical role of phospholipid phosphatidylinositol (4,5)-bisphosphate in maintaining the characteristics of epithelial cells.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Pablo Lazcano, Michael W. Schmidtke, Chisom J. Onu, Miriam L. Greenberg
Summary: This study demonstrates for the first time that modulation of phosphatidic acid (PA) levels through the AMPK-PLD pathway regulates IP6K1-mediated repression of myo-inositol-3-P synthase (MIPS).
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Chemistry, Organic
Cheng-Gang Qian, Bin Xiao
Summary: In this study, a new method for the synthesis of 4,5-disubstitued pyrene from phenangermoles via a Pd-catalyzed annulative pi-extension reaction was reported. Discriminated 4- and 5-position-substituted pyrene derivatives were achieved, showing the selectivity between phenangermoles and dibenzosiloles, as well as the reactivity difference between phenangermoles and phenansiloles. Successful transformation of arylalkylacetylenes under standard conditions has not been reported previously. The surprising compatibility of propargyl bromide allows the product to contain the benzyl bromide group, providing a convenient route for further derivatization.
ORGANIC CHEMISTRY FRONTIERS
(2023)
Article
Chemistry, Multidisciplinary
Rui Zhang, Ying Xia, Guangbin Dong
Summary: The study presents a [5+2] cycloaddition reaction catalyzed by Rh, which activates C-C bonds to enable the cyclization of 1-indanones and internal alkynes. The reaction demonstrates tolerance towards various functional groups and provides a direct route to diverse benzocycloheptenones. DFT studies suggest that migration insertion is the turnover-limiting step, with beneficial pi-pi interactions in the transition states.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Dermatology
Kaori Kanemaru, Ayano Matsuyuki, Yoshikazu Nakamura, Kiyoko Fukami
EXPERIMENTAL DERMATOLOGY
(2015)
Review
Biochemistry & Molecular Biology
Yoshikazu Nakamura, Kiyoko Fukami
JOURNAL OF BIOCHEMISTRY
(2017)
Article
Biochemistry & Molecular Biology
Zen Kouchi, Takahiro Igarashi, Nami Shibayama, Shunichi Inanobe, Kazuyuki Sakurai, Hideki Yamaguchi, Toshifumi Fukuda, Shigeru Yanagi, Yoshikazu Nakamura, Kiyoko Fukami
JOURNAL OF BIOLOGICAL CHEMISTRY
(2011)
Article
Cell Biology
Hideki Yamaguchi, Shuhei Yoshida, Emi Muroi, Nachi Yoshida, Masahiro Kawamura, Zen Kouchi, Yoshikazu Nakamura, Ryuichi Sakai, Kiyoko Fukami
JOURNAL OF CELL BIOLOGY
(2011)
Article
Neurosciences
Kim A. Staats, Lawrence Van Helleputte, Ashley R. Jones, Andre Bento-Abreu, Annelies Van Hoecke, Aleksey Shatunov, Claire L. Simpson, Robin Lemmens, Tom Jaspers, Kiyoko Fukami, Yoshikazu Nakamura, Robert H. Brown, Philip Van Damme, Adrian Liston, Wim Robberecht, Ammar Al-Chalabi, Ludo Van Den Bosch
NEUROBIOLOGY OF DISEASE
(2013)
Article
Cell Biology
Y. Nakamura, K. Kanemaru, R. Kojima, Y. Hashimoto, T. Marunouchi, N. Oka, T. Ogura, K. Tanonaka, K. Fukami
CELL DEATH & DISEASE
(2014)
Article
Multidisciplinary Sciences
Kaori Kanemaru, Yoshikazu Nakamura, Kojiro Sato, Ryota Kojima, Saori Takahashi, Mami Yamaguchi, Manabu Ichinohe, Hiroshi Kiyonari, Go Shioi, Kenji Kabashima, Kyoko Nakahigashi, Masataka Asagiri, Colin Jamora, Hideki Yamaguchi, Kiyoko Fukami
NATURE COMMUNICATIONS
(2012)
Article
Biochemistry & Molecular Biology
Kanako Shiratori, Kaori Kanemaru, Takahiro Ogura, Aya Nakajima, Yuko Sugizaki, Takatsugu Fukuyama, Yoichiro Iwakura, Yoshikazu Nakamura, Kiyoko Fukami
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2019)
Article
Dermatology
Takatsugu Fukuyama, Yoshikazu Nakamura, Kaori Kanemaru, Chiho Toyoda, Hyun-Jun Jang, Pann-Ghill Suh, Kiyoko Fukami
EXPERIMENTAL DERMATOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Atsuko Yoneda, Kaori Kanemaru, Ai Matsubara, Erika Takai, Makoto Shimozawa, Reiko Satow, Hideki Yamaguchi, Yoshikazu Nakamura, Kiyoko Fukami
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2020)
Article
Multidisciplinary Sciences
Yoshikazu Nakamura, Kaori Kanemaru, Madoka Shoji, Kengo Totoki, Karen Nakamura, Hidemasa Nakaminami, Keisuke Nakase, Norihisa Noguchi, Kiyoko Fukami
SCIENTIFIC REPORTS
(2020)
Article
Neurosciences
Sung Jin Park, Kirk D. Haan, Yoshikazu Nakamura, Kiyoko Fukami, Thomas E. Fisher
Summary: The study revealed that the PLC delta 1 isoform plays a crucial role in the activation of Delta N-TRPV1 channels and osmosensory transduction in MNCs, with its absence leading to impaired MNC function. This research advances our understanding of the molecular mechanisms of osmoregulation in mammals.
JOURNAL OF NEUROSCIENCE
(2021)
Article
Multidisciplinary Sciences
Kazuya Tsujita, Reiko Satow, Shinobu Asada, Yoshikazu Nakamura, Luis Arnes, Keisuke Sako, Yasuyuki Fujita, Kiyoko Fukami, Toshiki Itoh
Summary: Changes in cell mechanics contribute to cancer cell dissemination. High plasma membrane (PM) tension inhibits cancer dissemination by counteracting mechanosensitive BAR family protein assembly, while restoration of PM tension phenotypically convert malignant cells into a non-motile epithelial cell state.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Kaori Kanemaru, Makoto Shimozawa, Manabu Kitamata, Rikuto Furuishi, Hinako Kayano, Yui Sukawa, Yuuki Chiba, Takatsugu Fukuyama, Junya Hasegawa, Hiroki Nakanishi, Takuma Kishimoto, Kazuya Tsujita, Kazuma Tanaka, Toshiki Itoh, Junko Sasaki, Takehiko Sasaki, Kiyoko Fukami, Yoshikazu Nakamura
Summary: This study reveals the critical role of phospholipid phosphatidylinositol (4,5)-bisphosphate in maintaining the characteristics of epithelial cells.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Kaori Kanemaru, Yoshikazu Nakamura, Kengo Totoki, Takatsugu Fukuyama, Madoka Shoji, Hisae Kaneko, Kanako Shiratori, Atsuko Yoneda, Takafumi Inoue, Yoichiro Iwakura, Kenji Kabashima, Kiyoko Fukami
CELL DEATH AND DIFFERENTIATION
(2017)
Review
Biochemistry & Molecular Biology
Xin Zhang, Tian Yuan, Xuhui Chen, Xuebo Liu, Jun Hu, Zhigang Liu
Summary: The prevalence of Alzheimer's disease is increasing due to aging population, and ApoE4 allele is identified as the primary genetic risk factor. However, emerging research suggests that dietary factors, such as DHA, can modify the risk of AD. The interplay between DHA and ApoE genotype within the brain needs further investigation. Early DHA supplementation may benefit cognitively normal older adults carrying the ApoE4 gene, while its effectiveness for AD patients is limited.
PROGRESS IN LIPID RESEARCH
(2024)
Review
Biochemistry & Molecular Biology
Chen Wu, Haiqin Chen, Yongchao Mei, Bo Yang, Jianxin Zhao, Catherine Stanton, Wei Chen
Summary: This review summarizes the recent advances in microbial conjugated linoleic acid (CLA) production, including the mechanisms of linoleic acid conversion to CLA, factors influencing CLA production and isomer composition, and strategies for enhancing CLA content and nutritional value in fermented products using microorganisms. The review aims to serve as a reference for microbial CLA production and expand the understanding of the potential probiotic role of microbial CLA producers.
PROGRESS IN LIPID RESEARCH
(2024)