期刊
PROCESS BIOCHEMISTRY
卷 48, 期 5-6, 页码 988-993出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.procbio.2013.04.013
关键词
Iron-chelating peptides; Bioavailability; Iron; Ser-Cys-H is; Immobilized metal affinity chromatography (IMAC); Tandem mass spectrum (MS/MS)
资金
- Ocean Commonweal Foundation [201105029, 201205027]
- China Postdoctoral Science Foundation [2012M511549]
- Natural Science Foundation of China [30871943]
- Fundamental Research Funds for the Central Universities [201313002]
- China Scholarship Council (CSC)
Alaska pollock skin as aquatic by-product was usually discarded in food processing. Iron deficiency has been a nutritional problem for a long period. In this study, Alaska pollock skin was used to generate iron-chelating peptides after being treated by commercial enzymes. Sequential chromatography, including immobilized metal affinity chromatography (IMAC) and reversed phase high performance liquid chromatography (RP-HPLC), was used for capturing and purifying iron-chelating peptides. One tripeptide, with high iron-chelating activity, was obtained and identified by electrospray ionization tandem mass spectrometry (LC ESI-MS/MS). The amino acid sequence of the iron-chelating peptide was identified to be Ser-Cys-His (MW: 345 Da). These results show that Alaska pollock skin could be utilized to generate iron-chelating peptides as an iron supplement in functional food industry. (C) 2013 Elsevier Ltd. All rights reserved.
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