期刊
PROCESS BIOCHEMISTRY
卷 47, 期 11, 页码 1532-1538出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.procbio.2012.01.022
关键词
Metabolic flux quantification; Escherichia coli; Succinate production; Aerobic condition
资金
- National Basic Research Program of China (973 Program) [2012CB721100]
- National Special Fund for State Key Laboratory of Bioreactor Engineering [2060204]
- Shanghai Pujiang Program [08PJ14038]
- SRF for ROCS, SEM
- Shanghai Leading Academic Discipline Project [B505]
The production of succinate by engineered Escherichia coli strains has been widely investigated. In this study, quantitative comparison of metabolic fluxes was carried out for the wild-type E. coli strain and a quintuple mutant strain QZ1111 that was designed for the production of succinate aerobically by knocking out five genes (ptsG, poxB, pro, sdhA, iclR) of the wild-type E. coli MG1655. Metabolic flux distributions of both strains were quantified by C-13-labeling experiments, together with the determination of physiological parameters and the expression level of key genes. The experimental results indicated that under the same aeration condition the fraction of oxaloacetate molecules originating from phosphoenolpyruvate was increased in E. coli QZ1111 compared to that in the wild-type E. coli MG1655. The glyoxylate shunt was likely activated in E. coli QZ1111 only under high aeration condition but repressed in other conditions, indicating that the deletion of the iclR gene could not completely remove the repression of the glyoxylate shunt with limited oxygen supply. Our results also suggested further genetic manipulation strategies to enhance the production yield of succinate. (C) 2012 Elsevier Ltd. All rights reserved.
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