Article
Plant Sciences
Jenna Y. Y. Schambach, Colin P. S. Kruse, Peter Kitin, Wittney Mays, Christopher G. G. Hunt, Shawn R. R. Starkenburg, Amanda N. N. Barry
Summary: The potential benefits of using raw, non-food, lignocellulosic plant material as a carbon source for mixotrophic growth of microalgae have been demonstrated. This approach has advantages over using traditional carbon sources and has shown to increase the growth rate and biomass accumulation of Scenedesmus obliquus when cultured in the presence of switchgrass or yard waste.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Food Science & Technology
Raquel Guidetti Vendruscolo, Mariany Costa Depra, Pricila Nass Pinheiro, Valcenir Junior Mendes Furlan, Juliano Smanioto Barin, Alexandre Jose Cichoski, Cristiano Ragagnin de Menezes, Leila Queiroz Zepka, Eduardo Jacob-Lopes, Roger Wagner
Summary: This study evaluated the food potential of Scenedesmus obliquus biomass obtained from photosynthetic cultures enriched with different concentrations of carbon dioxide (CO2). The results showed that higher concentrations of CO2 led to increased synthesis of amino acids and proteins in the cultures. In addition, higher concentrations of chlorophylls were found in cultures with higher CO2 levels, while carotenoid concentrations were higher in cultures with lower CO2 levels. Overall, the Scenedesmus obliquus biomass accumulated significant amounts of proteins, lipids, and pigments through photosynthesis.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Multidisciplinary Sciences
Farouk K. El-Baz, Abeer Salama, Sami Ali, Rania Elgohary
Summary: Lutein, isolated from Scenedesmus obliquus, showed its effectiveness as an immunostimulant against cyclophosphamide-induced brain injury by reducing neuroinflammation, chemokines recruitment, and neuron degeneration with the modulation of immune markers.
SCIENTIFIC REPORTS
(2022)
Review
Chemistry, Medicinal
Anagha Nair, Ankesh Ahirwar, Shashikala Singh, Reeta Lodhi, Aishwarya Lodhi, Anshuman Rai, Dipak A. Jadhav, Harish, Sunita Varjani, Gurpreet Singh, Justine Marchand, Benoit Schoefs, Vandana Vinayak
Summary: Natural astaxanthin is richer than synthetic astaxanthin, but cultivating it in photobioreactors is expensive and downstream processing techniques for solubilization are not cost-effective. As a result, the cost of astaxanthin is high, leading pharmaceutical and nutraceutical companies to opt for synthetic astaxanthin. This review discusses the chemical character of astaxanthin, more cost-effective cultivating techniques, and its bioavailability. Moreover, the antioxidant properties of this microalgal product against various diseases are discussed, which make it a promising drug for reducing inflammation and its consequences.
Article
Agricultural Engineering
Rajesh Kona, Pavankumar Pallerla, Ramunaidu Addipilli, Prabhakar Sripadi, S. Venkata Mohan
Summary: The study evaluated the biosynthesis of lutein and beta-carotene by Scenedesmus sp. SVMIICT1 under different light intensities, finding that high lutein content was observed at 50 and 500 mu E/m(2)/s conditions. The study also showed that light intensities altered photosystem II and photosystem I in the algae.
BIORESOURCE TECHNOLOGY
(2021)
Article
Energy & Fuels
Hakimeh Mansouri, Sahar Ebrahim Nezhad, Hesam Kamyab, Shreeshivadasan Chelliapan, Irina Kirpichnikova
Summary: The mixotrophic growth of microalgae, specifically using acetate and pyruvate as carbon sources, has been found to significantly increase the biomass and biochemical parameters of Scenedesmus obliquus. Aeration has different effects on mixotrophic cultures, with acetate leading to a decline in biomass and biochemical parameters, while pyruvate showing enhancement effects. The fatty acid composition of the algae also changes under different growth conditions.
BIOMASS CONVERSION AND BIOREFINERY
(2022)
Article
Environmental Sciences
Amanda Dias, Alisson Carraro Borges, Andre Pereira Rosa, Marcio Aredes Martins
Summary: The study evaluated the efficiency of biomass separation from Scenedesmus obliquuos using coagulation-flocculation with natural coagulants like Moringa oleifera and Guazuma ulmifolia. Results showed promising potential for these natural coagulants in reducing the use of synthetic or metallic products in microalgae recovery processes.
Article
Agriculture, Multidisciplinary
Maria Teresa Senatore, Antonio Prodi, Francesco Tini, Virgilio Balmas, Alessandro Infantino, Andrea Onofri, Eleonora Cappelletti, Safa Oufensou, Michael Sulyok, Lorenzo Covarelli, Giovanni Beccari
Summary: This study assessed the evolution of fungal communities associated with durum wheat using different diagnostic approaches. The results showed that qPCR was the best method for predicting mycotoxin contamination in grains for some Fusarium species. The presence of Alternaria and Fusarium mycotoxins, particularly enniatins, was found in the grain harvested in central Italy, while deoxynivalenol was mainly detected in northern-central Italy.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2023)
Article
Biochemistry & Molecular Biology
Zhehua Chen, Gan Gu, Ziyun Wang, Dong Ou, Xianrui Liang, Changwei Hu, Xi Li
Summary: Tetracycline antibiotics can have toxic effects on freshwater microalgae, but at lower concentrations, the microalgae are able to recover and show enhanced photosynthetic activities. The transcriptomic analysis revealed differentially expressed genes related to photosynthesis. This study provides insights into the environmental risk of Tetracycline antibiotics and their effects on photosynthesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Yury Kostyukevich, Elena Stekolshikova, Anna Levashova, Anna Kovalenko, Anna Vishnevskaya, Anton Bashilov, Albert Kireev, Boris Tupertsev, Lidiia Rumiantseva, Philipp Khaitovich, Sergey Osipenko, Eugene Nikolaev
Summary: The administration of low doses of D2O has been used for decades to investigate metabolic pathways and measure compound turnover rate. Previously, the uptake of deuterium in lipids was mainly studied using GC-MS instruments, but recent untargeted LC-MS/MS lipidomics approaches have been employed. In this study, the deuterium uptake in over 500 lipids was investigated in 13 organs and body fluids of mice after 4 days of 100% D2O administration. The highest deuteration was observed in the liver, plasma, and lung, while lower levels were found in the brain and heart. The incorporation of deuterium in palmitic and stearic fragments in lipids was demonstrated using MS/MS, but not in the corresponding free forms. The results were analyzed based on lipid metabolic pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Qingqing Xiang, Ying Zhou, Chengxia Tan
Summary: The enantioselective toxic effects of Prothioconazole (PTC) on Scendesmus obliquus (S. obliquus) were investigated. It was found that R-(-)-PTC had less impact on the growth and photosynthetic pigment contents of S. obliquus compared to Rac-PTC and S-(+)-PTC. This study provides important information for the ecological risk assessment of PTC.
Article
Food Science & Technology
Tatiele C. do Nascimento, Pricila N. Pinheiro, Andressa S. Fernandes, Daniella C. Murador, Bruna Neves, Cristiano R. de Menezes, Veridiana V. de Rosso, Eduardo Jacob-Lopes, Leila Q. Zepka
Summary: The study found that the bioaccessibility was highest in ICE, with the most abundant carotenoid in Caco-2 cells being all-trans-beta-cryptoxanthin, followed by all-trans-echinenone. Generally, xanthophylls were uptake in greater proportion than carotenes.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Chemistry, Analytical
Zhendong Chen, Liang Li, Yan Zhan, Yifan Zhang, Haiyan Liu, Jianjun Zou, Dafang Zhong
Summary: Henagliflozin is a specific inhibitor of SGLT2 proposed for T2DM treatment. This study identified 8 metabolites of henagliflozin in human plasma and urine, focusing on the glucuronidation and O-deethylation pathways. A LC-MS/MS method was developed for quantification of M5-1, M5-2, and M5-3 in human plasma.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Environmental Sciences
Dongmei Xu, Yeting Xie, Jun Li
Summary: The antibiotic sulfamethoxazole (SMX) has been found to have biotoxic effects on Scenedesmus obliquus, an aquatic organism. Exposure to SMX promotes gene expression, leading to changes in algal cell ultrastructure. These changes include blurred cell walls, damaged chloroplast structure, and increased number and volume of mitochondria per cell. The effects of SMX on gene expression are related to inhibition of cell growth, decrease in chlorophyll content, increase in cell membrane permeability, production of reactive oxygen species, increased lipid peroxidation, and higher activities of antioxidant enzymes. The abnormal expression of non-coding RNAs may be an important mechanism underlying the carcinogenic effects of SMX.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Biotechnology & Applied Microbiology
Fernando Pagels, Helena M. Amaro, Tania G. Tavares, Susana Casal, F. Xavier Malcata, Isabel Sousa-Pinto, A. Catarina Guedes
Summary: Both light quality and irradiance have an influence on the productivity of biomass, fatty acids, carotenoids, and total antioxidant compounds in microalgae. The optimal condition for biomass productivity was found to be at 150 μmol(photons) m(-2) s(-1), regardless of light quality. However, for the production of carotenoids, polyunsaturated fatty acids, and/or total antioxidant capacity, the optimal condition shifted to 200 μmol(photons) m(-2) s(-1) under a specific light source.
JOURNAL OF APPLIED PHYCOLOGY
(2021)
Article
Plant Sciences
Benwen Liu, Jianhua Ge, Huan Zhu, Shuyin Li, Xiaoqi Dong, Guoxiang Liu, Zhengyu Hu
Summary: This study provides detailed information on the morphology, phylogenetic relationships, and plastome characteristics of Chaetophora lobata, aiding in evolutionary research of the genus. The identification of C. lobata is reliable through phylogenetic analyses based on nuclear rDNA and chloroplast protein-coding genes.
EUROPEAN JOURNAL OF PHYCOLOGY
(2022)
Article
Microbiology
Benwen Liu, Huan Zhu, Xiaoqi Dong, Qiufeng Yan, Guoxiang Liu, Zhengyu Hu
Summary: This study identified suitable chloroplast molecular markers for phylogenetic analyses of Chaetophorales, indicating that the limited number of samples was not the reason for the unsupported phylogenetic relationships. This research provides insights for solving taxonomic problems of the order Chaetophorales.
JOURNAL OF EUKARYOTIC MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Qian Xiong, Yuxin Hu, Wenqi Lv, Qinghua Wang, Guoxiang Liu, Zhengyu Hu
Summary: The study identified five chloroplast genomes of Oedogonium species, revealing their structural conservation but variation in genome sizes, AT contents, introns, and repeats. Phylogenetic analyses suggested that both Oedocladium and Oedogonium are polyphyletic groups. The identification of a positively selected gene in certain species indicates adaptive evolution in terrestrial Oedogoniales taxa.
Article
Biotechnology & Applied Microbiology
Yu Wang, Jing Jia, Qinglei Chi, Yanhua Li, Hongxia Wang, Yingchun Gong, Guoxiang Liu, Zhengyu Hu, Danxiang Han, Qiang Hu
Summary: The filamentous microalga Oedocladium carolinianum shows high potential for efficient production of astaxanthin under stress culture conditions, making it a promising candidate for sustainable co-production of astaxanthin and fatty acids.
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
(2022)
Article
Plant Sciences
Jiao Fang, Benwen Liu, Guoxiang Liu, Heroen Verbruggen, Huan Zhu
Summary: This study sequenced six new Trentepohliales chloroplast genomes, including Cephaleuros and Trentepohlia species, revealing their large size without quadripartite structure. The chloroplast genes of Trentepohliales exhibit elevated rates of evolution, strong rearrangement dynamics, and alternative genetic code assignments for some genes.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Ecology
Jiao Fang, Yangliang Chen, Guoxiang Liu, Heroen Verbruggen, Huan Zhu
Summary: Studies have shown a positive relationship between cell size and chloroplast genome size in chloroplast-bearing protists. The size of chloroplast genomes varies significantly between green algae in freshwater, marine, and terrestrial habitats, with terrestrial algae generally having larger genomes. The length of intergenic regions is a key factor contributing to the larger genomes in terrestrial species. Additionally, there are differences in chloroplast genome size and GC content among different functional morphological categories of algae.
FRONTIERS IN ECOLOGY AND EVOLUTION
(2021)
Article
Biodiversity Conservation
Qian Xiong, Yuxin Hu, Xiaoqi Dong, Yangliang Chen, Guoxiang Liu, Zhengyu Hu
Summary: This study determined the transcriptomes of eight Oedogoniales species to analyze their phylogenetic and evolutionary relationships. It revealed that Oedogonium is polyphyletic and suggested multiple terrestrialization events in Oedogoniales.
Article
Plant Sciences
Qian Xiong, Jiaqing Wang, Yuxin Hu, Qinghua Wang, Guoxiang Liu, Zhengyu Hu
Summary: This study determined and compared the mitochondrial genomes of Oedogoniales species, revealing differences between species and questioning the validity of traditional taxonomy. The findings also suggest rapid evolution in Oedocladium and Bulbochaete species.
EUROPEAN JOURNAL OF PHYCOLOGY
(2023)
Article
Plant Sciences
Danyang Ge, Simeng Zhang, Hongyan Liu, Yan Liu, Patrick John Kociolek, Huan Zhu, Guoxiang Liu, Yawen Fan
Summary: This study describes a new species of Achnanthidium, a diatom found in Tibet, which exhibits unique features compared to other known taxa.
Article
Biodiversity Conservation
Xiaoqi Dong, Huan Zhu, Xiong Xiong, Guoxiang Liu
Summary: In this study, the composition and diversity of the eukaryotic phytoplankton community in the Golmud area were examined using microscopic observation and high-throughput sequencing technology. The results showed that the community was diverse, with Bacillariophyta and Chlorophyta as the dominant phyla. Salinity was found to be the most influential environmental factor affecting the community structure.
Article
Genetics & Heredity
Benwen Liu, Yangliang Chen, Huan Zhu, Guoxiang Liu
Summary: This study used phylotranscriptomic analyses to compare the transcriptomes of Chaetophorales algal species and found consistent phylogenetic relationships with chloroplast genome data. The analysis also revealed expanded and contracted gene families and identified close relationships between certain species.
Article
Biodiversity Conservation
Qiufeng Yan, Qingyu Dai, Benwen Liu, Guoxiang Liu, Huan Zhu
Summary: No Ulvella species have been found in the Qinghai-Tibetan Plateau. In this study, three isolated strains from Qinghai Lake were identified as potentially new Ulvella species based on phylogenetic analysis and morphological differences.
Article
Plant Sciences
Qian Xiong, Yangliang Chen, Qingyu Dai, Benwen Liu, Guoxiang Liu
Summary: In this study, a phylogenetic analysis of the genus Oedogonium was conducted using morphological and molecular data. The results suggest that the traditional taxonomy of this genus may not accurately reflect its phylogeny. Additionally, a new taxonomical classification based on the shapes of basal cells was proposed.
Article
Genetics & Heredity
Ben -Wen Liu, Shu-Yin Li, Qiu-Feng Yan, Huan Zhu, Guo-Xiang Liu
Summary: This study deconstructed the taxonomic scheme of Watanabeales and revealed the high cryptic diversity of this group through comparative genomic analysis. The results enrich the chloroplast genome resources of Watanabeales, clarify the phylogenetic status of species within this order, and provide more reference information for subsequent taxonomic and phylogenetic study.
Article
Plant Sciences
Ben -Wen Liu, Shu-Yin Li, Huan Zhu, Guo-Xiang Liu
Summary: This study aims to identify the environmental factors that drive phyllosphere algal community composition and diversity in rainforests. The researchers used single molecule real-time sequencing to characterize the phyllosphere microalgal communities growing on four common tree species in three types of forest. The study found that forest type and host tree species significantly influenced the structure of the algal community, and that soluble reactive phosphorous, total nitrogen, and ammonium contents were important environmental factors affecting the communities.
