Article
Biochemistry & Molecular Biology
Hiromitsu Suzuki, Toshiki Morishima, Atsuya Handa, Hironaka Tsukagoshi, Masashi Kato, Motoyuki Shimizu
Summary: Pectinolytic enzymes, including pectate lyases, have diverse industrial applications. In this study, the biochemical characterization of the pectate lyase AnPL9 from the filamentous fungus Aspergillus nidulans was performed. AnPL9 exhibited high activity on various pectin substrates and showed stability within a neutral-to-alkaline pH range. These findings suggest that AnPL9 has potential biotechnological applications in the food, paper, and textile industries.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Kanchan Yadav, Shruti Dwivedi, Supriya Gupta, Aiman Tanveer, Sangeeta Yadav, Pramod K. Yadav, Gautam Anand, Dinesh Yadav
Summary: Pectin, a fundamental component of plant cell walls, is degraded by pectin lyases which have unique characteristics and play important roles in various industries such as juice processing. This review provides updates on the sources, production strategies, biochemical attributes, and molecular insights of pectin lyases.
PROCESS BIOCHEMISTRY
(2023)
Article
Food Science & Technology
Godfrey Wokorach, Sofie Landschoot, Amerida Lakot, Sidney Arihona Karyeija, Kris Audenaert, Richard Echodu, Geert Haesaert
Summary: Human exposure to aflatoxins in food can lead to acute stunting in children, liver cancer, and death. The severity of aflatoxin contamination depends on the type of Aspergillus fungus infecting the crops. Evaluating the ability of non-aflatoxigenic chemotypes to reduce aflatoxin levels produced by aflatoxigenic strains could be crucial in mitigating field contamination of crops with aflatoxins.
Article
Agriculture, Multidisciplinary
Wenhui Wei, Faxiu Lan, Yinghang Liu, Lianghuan Wu, Bachar H. Hassan, Shihua Wang
Summary: The bifunctional gene bioDA in Aspergillus flavus plays a crucial role in biotin biosynthesis and can complement E. coli mutants. The gene product is mainly localized to the mitochondria, unaffected by environmental stresses, and its deletion impairs biotin production and reduces pathogenicity. This study sheds light on biotin biosynthesis in A. flavus and identifies potential antifungal drug candidates.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
Mahmoud H. Hendy, Amr H. Hashem, Waleed B. Sulieman, Mahmoud H. Sultan, Mohamed Abdelraof
Summary: L-methionine gamma-lyase (MGL) was purified from A. fumigatus using heat treatment and gel filtration, resulting in 3.04-fold purification and 73.9% enzymatic recovery. The purified MGL showed a molecular mass of approximately 46 KDa. It exhibited maximum activity at pH 7 and stability within the pH range of 5.0-7.5. The enzyme had the highest catalytic activity at 30-40°C and remained stable up to 40°C. It was significantly inhibited by several metal ions and chemical reagents. MGL demonstrated anticancer activity against Hep-G2 and HCT116 cell lines at low concentrations. Overall, A. fumigatus MGL possesses favorable catalytic properties and potential for enzymotherapy.
MICROBIAL CELL FACTORIES
(2023)
Article
Biochemistry & Molecular Biology
Vishal Gupta, Prem Pratap Singh, Bhanu Prakash
Summary: This study reports the antifungal and AFB1 inhibitory effects of a formulation CIM developed using a mixture of essential oils and methyl anthranilate. The mechanism of action was explored through biochemical, molecular docking, and RT-PCR analysis. Furthermore, nanoencapsulation of CIM was prepared and shown to effectively control A. flavus and AFB1 contamination, and protect the active ingredients of herbal raw materials without affecting their sensory properties. Overall, this research provides a green chemical agent to protect the biodeterioration of active ingredients caused by toxigenic species of A. flavus.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Applied
Jessika N. Humerez-Flores, Clare Kyomugasho, Ana A. Gutierrez-Ortiz, Margot De Bie, Agnese Panozzo, Ann M. Van Loey, Paula Moldenaers, Marc E. Hendrickx
Summary: Tailored citrus pectin-derived compounds were produced through controlled enzymatic and chemical modifications, yielding compounds with different degrees of methylesterification and molecular weights. These compounds will be further studied for exploring their functionality.
Article
Biochemistry & Molecular Biology
Parisa Fathi Rezaei, Shahab Ghanbari, Gholamreza Mahdavinia
Summary: The kappa carrageenan-chitosan nanomagnetics (CCMNs) and chitosan-pectin nanomagnetics (CPMNs) were synthesized and characterized. The exo-polygalacturonase (exo-PG) was purified and immobilized on CCMNs and CPMNs by physical adsorption. The immobilized Exo-PG on CPMNs and CCMNs beads showed retained activity after multiple cycles, indicating their potential application in food industries.
PROCESS BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Yahya S. Alqahtani, Sunil S. More, R. Keerthana, Ibrahim Ahmed Shaikh, K. J. Anusha, Veena S. More, Francois N. Niyonzima, Uday M. Muddapur, Aejaz A. Khan
Summary: This study isolated a bacterium that produces pectinase from soil and identified it as Bacillus subtilis 15A B-92. The purified pectinase showed high specific activity and purity. The pectinase exhibited optimal activity and stability under specific temperature and pH conditions. The pectinase produced by this bacterium has high degradation ability towards citrus pectin and can be applied in juice clarification.
Article
Biotechnology & Applied Microbiology
Yu-Liang Qin, Shuai-Bing Zhang, Yang-Yong Lv, Huan-Chen Zhai, Yuan-Sen Hu, Jing-Ping Cai
Summary: This study provides new insights into the antifungal mechanisms of 1-nonanol against A. flavus, showing that 1-nonanol can distort spore morphology, induce increased membrane permeability, damage DNA, and exert destructive effects on A. flavus spores.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Polymer Science
Lingling Liu, Kaleb D. Fisher, Mason A. Friest, Gina Gerard
Summary: A carboxylated nanocellulose derived from corn stover was successfully prepared and used as a stabilizer for lemongrass essential oil-loaded emulsions. The nanoemulsion stabilized by this carboxylated nanocellulose exhibited a small particle size and good thermodynamic stability, and showed enhanced antifungal activity.
Article
Biotechnology & Applied Microbiology
Anurag Kumar, Jinglin Li, Sanath Kondaveeti, Bakul Singh, Ramasamy Shanmugam, Vipin Chandra Kalia, In-Won Kim, Jung-Kul Lee
Summary: This article presents a study on a NAD(+)-dependent xylitol dehydrogenase (AfXDH) from A. flavus, including its enzymatic activity, thermal stability, and application in l-xylulose production.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Food Science & Technology
Julius P. Sserumaga, John M. Wagacha, Moses Biruma, Charity K. Mutegi
Summary: The study assessed contamination of groundnut with Aspergillus section Flavi fungi and aflatoxin under storage conditions in Uganda. Different cropping systems, storage, and climatic conditions influenced the composition of fungal communities and aflatoxin levels. Urgent management strategies are required for districts with extremely high aflatoxin levels in groundnut samples.
Article
Chemistry, Applied
Linlin Li, Ziqian Li, Thomas Balle, Guoqin Liu, Zheng Guo
Summary: This study synthesized a new multipurpose amphiphilic POS by coupling depolymerized citrus pectin with alkyl succinylation. The resulting POS showed excellent performance in stabilizing oil/water emulsions and other extensive applications.
FOOD HYDROCOLLOIDS
(2023)
Article
Chemistry, Physical
Raul T. Rodrigues, Jose R. Siqueira Jr., Luciano Caseli
Summary: The catalytic activity of enzymes immobilized in LB films can be influenced by the insertion of carbon nanotubes, leading to enhanced stability and rheological properties of the system. The enzyme-CNT-lipid film showed at least twice the catalytic activity compared to pure enzyme, demonstrating the potential for an alternative bio-inspired film with high control over molecular architecture using the LB approach.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Pramod K. Yadav, Michael Martinov, Victor Vitvitsky, Javier Seravalli, Rudolf Wedmann, Milos R. Filipovic, Ruma Banerjee
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2016)
Article
Chemistry, Multidisciplinary
Rudolf Wedmann, Constantin Onderka, Shengwei Wei, Istvan Andras Szijarto, Jan Lj Miljkovic, Aleksandra Mitrovic, Mike Lange, Sergey Savitsky, Pramod Kumar Yadav, Roberta Torregrossa, Ellen G. Harrer, Thomas Harrer, Isao Ishii, Maik Gollasch, Mark E. Wood, Erwan Galardon, Ming Xian, Matthew Whiteman, Ruma Banerjee, Milos R. Filipovi
Article
Biochemistry & Molecular Biology
Victor Vitvitsky, Pramod K. Yadav, Sojin An, Javier Seravalli, Uhn-Soo Cho, Ruma Banerjee
JOURNAL OF BIOLOGICAL CHEMISTRY
(2017)
Article
Biochemistry & Molecular Biology
Victor Vitvitsky, Jan Lj Miljkovic, Trever Bostelaar, Bikash Adhikari, Pramod K. Yadav, Andrea K. Steiger, Roberta Torregrossa, Michael D. Pluth, Matthew Whiteman, Ruma Banerjee, Milos R. Filipovic
ACS CHEMICAL BIOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Pramod K. Yadav, Victor Vitvitsky, Hanseong Kim, Andrew White, Uhn-Soo Cho, Ruma Banerjee
JOURNAL OF BIOLOGICAL CHEMISTRY
(2019)
Article
Biochemistry & Molecular Biology
Pramod Kumar Yadav, Victor Vitvitsky, Sebastian Carballal, Javier Seravalli, Ruma Banerjee
JOURNAL OF BIOLOGICAL CHEMISTRY
(2020)
Article
Biochemistry & Molecular Biology
Lauren E. Williamson, Theron Gilliland, Pramod K. Yadav, Elad Binshtein, Robin Bombardi, Nurgun Kose, Rachel S. Nargi, Rachel E. Sutton, Clarissa L. Durie, Erica Armstrong, Robert H. Carnahan, Lauren M. Walker, Arthur S. Kim, Julie M. Fox, Michael S. Diamond, Melanie D. Ohi, William B. Klimstra, James E. Crowe
Article
Multidisciplinary Sciences
Sarah M. Fantin, Kristine F. Parson, Pramod Yadav, Brock Juliano, Geoffrey C. Li, Charles R. Sanders, Melanie D. Ohi, Brandon T. Ruotolo
Summary: Peripheral myelin protein (PMP22) misfolding is identified as a key factor in various peripheral neuropathies. Mutant forms of PMP22 exhibit differences in stability and propensity to form homodimeric complexes compared to wild-type protein. The formation of PMP22 dimers from destabilized monomers is proposed as a key element in PMP22 mistrafficking, based on combined findings from native ion mobility-mass spectrometry and cellular data.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Agronomy
Shubhra Gupta, Kapil Gupta, Chitra Nehra, R. K. Gaur, Dinesh Yadav
Summary: Sugarcane is a major cash crop globally, but it faces challenges from various stresses. Conventional breeding approaches have limitations, but recent advancements in omics-driven research provide new opportunities for developing salt-tolerant sugarcane varieties. The use of genomics, proteomics, and genome-editing technologies has greatly enhanced the pace of sugarcane improvement.
Article
Biotechnology & Applied Microbiology
Kanchan Yadav, Shruti Dwivedi, Supriya Gupta, Amit K. Dubey, Vinay K. Singh, Aiman Tanveer, Sangeeta Yadav, Dinesh Yadav
Summary: In this study, 52 pectin lyase genes were predicted and characterized in six species of Fusarium. The protein sequences showed variability in terms of amino acid residues, molecular weight, and pI. Multiple sequence alignment and phylogenetic analysis revealed conserved residues and distinct groups. Three-dimensional structure prediction and analysis showed diverse structural motifs. The study provides important insights into the diversity and potential applications of pectin lyase enzymes.
Article
Biochemistry & Molecular Biology
Surabhi Suchanti, Sonali Awasthi, Gyanendra Singh, Pramod K. Yadav, Abhijeet Singh, Rajeev Mishra
Summary: Lung cancer and COVID-19 present a severe combination due to the lungs being the primary target organ for SARS-CoV-2 infection. The study identified a potential correlation between immune-modulators and receptor proteins that requires further laboratory research for clinical validation.
BIOCHEMISTRY AND BIOPHYSICS REPORTS
(2022)
Review
Plant Sciences
Varsha Rani, D. C. Joshi, Priyanka Joshi, Rajesh Singh, Dinesh Yadav
Summary: The main purpose of this review is to shed light on the role of millet models in imparting climate resilience and nutritional security and to give a concrete perspective on how NF-Y transcription factors can be harnessed for making cereals more stress tolerant. Agriculture faces significant challenges from climate change, bargaining, population, elevated food prices, and compromises with nutritional value.
Review
Plant Sciences
Rupali Gupta, Gautam Anand, Rajeeva Gaur, Dinesh Yadav
Summary: Plant-microbiome interactions play a significant role in nutrient acquisition and defense mechanisms of the host plant. Understanding and utilizing plant microbiomes can have implications for future agricultural applications.
PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS
(2021)
Article
Biochemistry & Molecular Biology
Aaron P. Landry, Sojin Moon, Hanseong Kim, Pramod K. Yadav, Arkajit Guha, Uhn-Soo Cho, Ruma Banerjee
CELL CHEMICAL BIOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Markus Ruetz, Gregory C. Campanello, Liam McDevitt, Adam L. Yokom, Pramod K. Yadav, David Watkins, David S. Rosenblatt, Melanie D. Ohi, Daniel R. Southworth, Ruma Banerjee
CELL CHEMICAL BIOLOGY
(2019)
