Article
Developmental Biology
Anna L. F. Assumpcao, Guoping Fu, Deependra K. Singh, Zhanping Lu, Ashley M. Kuehnl, Rene Welch, Irene M. Ong, Renren Wen, Xuan Pan
Summary: YY1, a ubiquitous transcription factor and PcG protein, plays important roles in regulating lymphocyte development and stem cell self-renewal. The PcG function of YY1 is necessary for T and B cell development, but not for myeloid cells. YY1's role in early T cell development involves both PcG-dependent and -independent regulations, promoting cell survival and Notch1 signaling independently.
Article
Immunology
Klaus Tenbrock, Thomas Rauen
Summary: T cells play a crucial role in the development of SLE, and their abnormalities provide potential targets for therapeutic intervention.
CLINICAL IMMUNOLOGY
(2022)
Article
Biochemical Research Methods
Brian Reardon, Zeynep Kosaloglu-Yalcin, Sinu Paul, Bjoern Peters, Alessandro Sette
Summary: This study compared different strategies using common and allele-specific thresholds to identify an optimal approach for predicting T-cell epitopes. Results showed that allele-specific threshold performance was comparable to that of a common threshold when considering a relatively large number of alleles, but using allele-specific thresholds would be preferable when predicting epitopes for only a few alleles. A set of allele-specific thresholds for identifying T-cell epitopes at 80% sensitivity was provided for public use.
MOLECULAR & CELLULAR PROTEOMICS
(2021)
Article
Multidisciplinary Sciences
Andrew C. Yang, Fabian Kern, Patricia M. Losada, Maayan R. Agam, Christina A. Maat, Georges P. Schmartz, Tobias Fehlmann, Julian A. Stein, Nicholas Schaum, Davis P. Lee, Kruti Calcuttawala, Ryan T. Vest, Daniela Berdnik, Nannan Lu, Oliver Hahn, David Gate, M. Windy McNerney, Divya Channappa, Inma Cobos, Nicole Ludwig, Walter J. Schulz-Schaeffer, Andreas Keller, Tony Wyss-Coray
Summary: This study found that patients with COVID-19 exhibited widespread cellular perturbations in the brain, including the relay of peripheral inflammation into the brain by choroid plexus barrier cells and infiltration of peripheral T cells. Additionally, COVID-19 affected synaptic signaling of upper-layer excitatory neurons linked to cognitive function.
Article
Cell Biology
Jongmin J. Kim, Emma R. Steinson, Mei Sheng Lau, Dirk G. de Rooij, David C. Page, Robert E. Kingston
Summary: This study examines the role of CBX2 in male germ cell development and its nonenzymatic role in regulating the transcriptional repression of stem cell genes. CBX2 forms condensates and binds chromatin to compact it and create a repressed physical environment that promotes spermatogonial differentiation. The research highlights the critical role of CBX2 in chromatin structure regulation and its importance in spermatogenesis.
GENES & DEVELOPMENT
(2023)
Article
Hematology
Roger Mulet-Lazaro, Stanley van Herk, Claudia Erpelinck, Eric Bindels, Mathijs A. Sanders, Carlo Vermeulen, Ivo Renkens, Peter Valk, Ari M. Melnick, Jeroen de Ridder, Michael Rehli, Claudia Gebhard, Ruud Delwel, Bas J. Wouters
Summary: The study found that allele-specific expression of the GATA2 gene is closely associated with CEBPA double mutations, and may be acquired through mechanisms such as promoter methylation and overactivation, playing an important role in the development of AML.
Article
Biochemistry & Molecular Biology
Bahire Kalfaoglu, Jose Almeida-Santos, Chanidapa Adele Tye, Yorifumi Satou, Masahiro Ono
Summary: Research indicates that T-cells play crucial roles in the immunity to COVID-19, with dysregulation noted in severe cases. The impairment of FOXP3 induction in CD4(+) T-cells may lead to abnormal T-cell responses, while hyperactivated T-cells could potentially contribute to dysregulation and tissue destruction in severe COVID-19 patients.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Guanghao Qi, Benjamin J. Strober, Joshua M. Popp, Rebecca Keener, Hongkai Ji, Alexis Battle
Summary: Differential allele-specific expression (ASE) is a powerful tool to study gene expression regulation, and single-cell RNA sequencing allows measurement of ASE at individual-cell resolution. However, there is a lack of statistical methods for analyzing such data. In this study, the authors develop a statistical method called DAESC for analyzing differential ASE using single-cell RNA-seq data, which can provide new insights into gene regulation.
NATURE COMMUNICATIONS
(2023)
Article
Biotechnology & Applied Microbiology
Steven J. Wu, Scott N. Furlan, Anca B. Mihalas, Hatice S. Kaya-Okur, Abdullah H. Feroze, Samuel N. Emerson, Ye Zheng, Kalee Carson, Patrick J. Cimino, C. Dirk Keene, Jay F. Sarthy, Raphael Gottardo, Kami Ahmad, Steven Henikoff, Anoop P. Patel
Summary: In this study, we successfully adapted the CUT&Tag method to single-cell platforms for profiling chromatin landscapes at single-cell level. The analysis of H3K27me3 using scCUT&Tag distinguished cell types and allowed generation of cell-type-specific PcG landscapes.
NATURE BIOTECHNOLOGY
(2021)
Article
Immunology
Brandilyn A. Peters, Jee-Young Moon, David B. Hanna, Olaf Kutsch, Margaret Fischl, Caitlin A. Moran, Adaora A. Adimora, Stephen Gange, Nadia R. Roan, Katherine G. Michel, Michael Augenbraun, Anjali Sharma, Alan Landay, Seema Desai, Robert C. Kaplan
Summary: Persistent CD4(+) T-cell activation and exhaustion may contribute to increased long-term mortality risk in women with HIV, independent of HIV disease progression.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Oncology
Sonia N. Wolf, Jana Haddow, Claire Greiller, Graham P. Taylor, Lucy B. M. Cook, Aileen G. Rowan
Summary: Detecting ATL early in HTLV-1 carriers through quantification of TCR V beta subunit diversity using OCI-flow can identify high-PVL carriers at increased risk of developing ATL. Those with an OCI-flow >0.770 have a higher likelihood of developing ATL and may benefit from intervention therapy.
BLOOD CANCER JOURNAL
(2021)
Article
Cell Biology
Changfu Yao, Stephanie A. Bora, Tanyalak Parimon, Tanzira Zaman, Oren A. Friedman, Joseph A. Palatinus, Nirmala S. Surapaneni, Yuri P. Matusov, Giuliana Cerro Chiang, Alexander G. Kassar, Nayan Patel, Chelsi E. R. Green, Adam W. Aziz, Harshpreet Suri, Jo Suda, Andres A. Lopez, Gislaine A. Martins, Barry R. Stripp, Sina A. Gharib, Helen S. Goodridge, Peter Chen
Summary: Recent studies have shown immunologic dysfunction in severely ill patients with COVID-19, particularly in those with ARDS, where defective antigen presentation and interferon responsiveness were observed in monocytes. There was also suppressed cytotoxic activity in NK cells and CD8 T lymphocytes, along with deficient B cell activation, indicating delayed viral clearance in severely ill patients. These findings suggest an immune imbalance in COVID-19 patients with ARDS, potentially contributing to a more severe disease course.
Article
Neurosciences
Sonia Malaiya, Marcia Cortes-Gutierrez, Brian R. Herb, Sydney R. Coffey, Samuel R. W. Legg, Jeffrey P. Cantle, Carlo Colantuoni, Jeffrey B. Carroll, Seth A. Ament
Summary: Research in a Huntington's disease mouse model revealed a pattern of bidirectional dysregulation in cell type-specific genes, possibly due to partial loss-of-function of the polycomb repressive complex 2, leading to deficits in cell identity maintenance across various cell types in the adult striatum.
JOURNAL OF NEUROSCIENCE
(2021)
Review
Biochemistry & Molecular Biology
Anna Mroczek, Joanna Zawitkowska, Jerzy Kowalczyk, Monika Lejman
Summary: Acute lymphoblastic leukaemia (ALL) is a common childhood cancer with a cure rate of over 80%, diagnosed based on various features. Despite unclear pathogenesis, research focuses on new therapeutic targets and predictive factors for improved patient outcomes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Hematology
Deepak Kumar, Thinh H. Nguyen, Carolyn M. Bennett, Chengyu Prince, Laura Lucas, Sunita Park, Taylor Lawrence, Karin Chappelle, Mariam Ishaq, Edmund K. Waller, Sampath Prahalad, Michael Briones, Shanmuganathan Chandrakasan
Summary: mTOR inhibitors like sirolimus are increasingly used to manage multilineage immune cytopenia (m-IC) in children. However, it is unclear how sirolimus affects the broader immune dysregulation associated with m-IC.
Article
Clinical Neurology
Lorraine Flet-Berliac, Nicolas Tchitchek, Anne Lepine, Anca Florea, Helene Maurey, Pascale Chretien, Salima Hacein-Bey-Abina, Frederic Villega, Emmanuel Cheuret, Veronique Rogemond, Geraldine Picard, Jerome Honnorat, Kumaran Deiva
Summary: This French study aimed to investigate the long-term clinical and cognitive outcomes of patients with anti-N-methyl-D-aspartate receptor encephalitis (NMDAR-E). The study found that more than 45% of patients experienced cognitive and academic difficulties, and the severity of symptoms seemed to have an impact on cognition and academic performances.
DEVELOPMENTAL MEDICINE AND CHILD NEUROLOGY
(2023)
Article
Oncology
David Jeremie Birnbaum, Maelle Picard, Quentin Da Costa, Thomas Delayre, Pascal Finetti, Olivier Cabaud, Emilie Agavnian, Bernadette De Rauglaudre, Emilie Denicolai, Francois Bertucci, Emilie Mamessier
Summary: Hepatocellular carcinoma (HCC) is a common and deadly cancer, and new treatments are needed. Immune checkpoint inhibitors (ICIs) have shown promise for HCC. By analyzing a large transcriptomic database, we found that the TIGIT/DNAM-1 axis, particularly the PVRIG molecule, could be a potential therapeutic option for HCC.
Article
Immunology
Bakhos Jneid, Aurore Bochnakian, Caroline Hoffmann, Fabien Delisle, Emeline Djacoto, Philemon Sirven, Jordan Denizeau, Christine Sedlik, Yohan Gerber-Ferder, Frederic Fiore, Ramazan Akyol, Carine Brousse, Robert Kramer, Ian Walters, Sylvain Carlioz, Helene Salmon, Bernard Malissen, Marc Dalod, Eliane Piaggio, Nicolas Manel
Summary: Activation of STING in immunogenic tumors can induce antitumor T cell response, but it remains unclear how to apply it in nonimmunogenic tumors. This study showed that intratumoral delivery of cGAMP-VLP activated T cells and reduced tumor regulatory T cells, synergizing with PD1 blockade. However, intratumoral administration of the synthetic CDN ADU-S100 caused tumor necrosis and systemic T cell activation, but depleted immune cells and minimally primed circulating tumor-specific T cells. Thus, cell targeting of STING stimulation shapes the antitumor T cell response and provides a strategy to enhance T cell-targeted immunotherapy.
SCIENCE IMMUNOLOGY
(2023)
Article
Biology
Warren Anderson, Fariba Barahmand-pour-Whitman, Peter S. Linsley, Karen Cerosaletti, Jane H. Buckner, David J. Rawlings, Bernard Malissen
Summary: A genetic variant in the PTPN22 gene increases the risk for multiple autoimmune diseases and affects T cell regulation and activation. Using gene editing, researchers created T cells with different PTPN22 variants and found that the risk variant and PTPN22 knockout both led to increased T cell activation. They also observed enhanced signaling and proliferation in T cells with self-reactive T cell receptors lacking PTPN22 function. These findings suggest that PTPN22 rs2476601 contributes to autoimmunity risk by promoting TCR signaling and activation in mildly self-reactive T cells.
Article
Oncology
Clemence Demerle, Laurent Gorvel, Marielle Mello, Sonia Pastor, Clara Degos, Ana Zarubica, Fabien Angelis, Frederic Fiore, Jacques A. Nunes, Bernard Malissen, Laurent Greillier, Geoffrey Guittard, Herve Luche, Fabrice Barlesi, Daniel Olive
Summary: The study found that anti-HVEM18-10 can enhance the activity of human αβ-T cells and trigger T cell activation in the presence of PD-L1-negative cells. Anti-HVEM18-10 treatment inhibits the growth of HVEM-positive tumors in mouse models and promotes the presence of effector memory CD4+ T cells while suppressing exhausted CD8+ T cells and regulatory T cells.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2023)
Article
Biology
Richard Miallot, Virginie Millet, Yann Groult, Angelika Modelska, Lydie Crescence, Sandrine Roulland, Sandrine Henri, Bernard Malissen, Nicolas Brouilly, Laurence Panicot-Dubois, Renaud Vincentelli, Gerlind Sulzenbacher, Pascal Finetti, Aurelie Dutour, Jean-Yves Blay, Francois Bertucci, Franck Galland, Philippe Naquet
Summary: Aggressive tumors often have dysfunctional mitochondria. OMA1 mediates fission of mitochondria in response to oxidative stress through cleavage of the fusion effector OPA1. In this study, mutation of cysteine 403 in OMA1 impaired mitochondrial responses to stress, resulting in reduced ATP production, resistance to apoptosis, and enhanced mitochondrial DNA release. Inactivation of OMA1 increased anti-tumor immunity and may enhance sarcoma immunogenicity.
LIFE SCIENCE ALLIANCE
(2023)
Article
Immunology
Anais Joachim, Rudy Aussel, Lena Gelard, Fanghui Zhang, Daiki Mori, Claude Gregoire, Sergio Villazala Merino, Mauro Gaya, Yinming Liang, Marie Malissen, Bernard Malissen
Summary: Mice with a loss-of-function mutation in the LAT adaptor (Lat(Y136F)) develop an autoimmune and type 2 inflammatory disorder called defective LAT signalosome pathology (DLSP). Through single-cell omics analysis, it was found that T follicular helper cells, CD4+ cytotoxic T cells, activated B cells, and plasma cells are present in Lat(Y136F) spleen and lung. This cell constellation is similar to the cell types involved in IgG4-related disease (IgG4-RD), indicating that Lat(Y136F) DLSP can serve as a model for IgG4-RD.
JOURNAL OF EXPERIMENTAL MEDICINE
(2023)
Article
Biology
Richard Miallot, Virginie Millet, Anais Roger, Romain Fenouil, Catherine Tardivel, Jean-Charles Martin, Laetitia Shintu, Paul Berchard, Juliane Sousa Lanza, Bernard Malissen, Sandrine Henri, Sophie Ugolini, Aurelie Dutour, Pascal Finetti, Francois Bertucci, Jean-Yves Blay, Franck Galland, Philippe Naquet
Summary: The study found that pantethine can inhibit tumor growth by enhancing the function of the immune system. Pantethine can promote the polarization of myeloid and dendritic cells towards enhanced antigen presentation pathways and improve the development of CD8+ T cells with immune effector activity. However, immune cell exhaustion limits the therapeutic effect of pantethine.
LIFE SCIENCE ALLIANCE
(2023)
Meeting Abstract
Oncology
Delphine Nicolle, Aurore Gorce, Marie Tavernier, Elisabetta Marangoni, Didier Decaudin, Christophe Ginestier, Emmanuelle Charafe-Jaufret, Judith Passildas, Nina Robin, Robert Clarke, Erwan Corcuff, Anais Joachim, Bernard Malissen, Ana Zarubica, Herve Luche, Jean-Gabriel Judde, Olivier Deas
Meeting Abstract
Oncology
Anais Joachim, Emilie Maturin, Marielle Mello, Lillia Hadjem, Magali Grange, Olivier Deas, Ana Zarubica, Bernard Malissen, Herve Luche
Meeting Abstract
Oncology
Herve Luche, Lillia Hadjem, Marielle Mello, Priscilla Canavese, Fabien Angelis, Anais Joachim, Sylvie Bouilly, Frederic Guinut, Frederic Fiore, Bernard Malissen, Ana Zarubica, Erwan Corcuff
Meeting Abstract
Oncology
Laurent Gorvel, Clemence Demerle, Marielle Mello, Sonia Pastor, Clara Degos, Ana Zarubica, Fabien Angelis, Frederic Fiore, Jacques Nunes, Bernard Malissen, Laurent Greillier, Geoffrey Guittard, Herve Luche, Fabrice Barlesi, Daniel Olive
Article
Immunology
Morgane Thomas, Charlotte Bruzeau, Ophelie Alyssa Martin, Justine Pollet, Sebastien Bender, Claire Carrion, Sandrine Le Noir, Eric Pinaud
Summary: SATB1 is a cell type-specific factor that plays an essential role in the genetic network of developing T cells and neurons. However, its involvement in B-cell differentiation needs further clarification. Our study using a SATB1 knockout mouse model reveals that SATB1 is dispensable for B-cell development and the establishment of a broad IgH repertoire. We also find that SATB1 has a dual function in mature B cells, acting as a positive regulator in naive cells and a negative regulator in activated cells.
CELLULAR & MOLECULAR IMMUNOLOGY
(2023)
Correction
Oncology
C. Demerle, L. Gorvel, M. Mello
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2023)
Article
Hematology
Elisa Gabinaud, Laurent Hannouche, Elisa Andersen, Delphine Bastelica, Denis Bernot, Manal Ibrahim-Kosta, Pierre-Emmanuel Morange, Marie Loosveld, Paul Saultier, Dominique Payet-Bornet, Marie-Christine Alessi, Delphine Potier, Marjorie Poggi
Summary: Using single-cell RNA sequencing, researchers have discovered a link between ETV6 germline mutations and thrombocytopenia through the highly deregulated core ribosome biogenesis pathway.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2023)