Article
Neurosciences
Kadidia Pemba Adula, Matthew Shorey, Vasudha Chauhan, Khaled Nassman, Shu-Fan Chen, Melissa M. Rolls, Alvaro Sagasti
Summary: DLK and LZK play different roles in zebrafish, promoting axon regeneration in motor neurons while inhibiting excessive growth in sensory neurons.
JOURNAL OF NEUROSCIENCE
(2022)
Article
Genetics & Heredity
Sucheta S. Kulkarni, Vidur Sabharwal, Seema Sheoran, Atrayee Basu, Kunihiro Matsumoto, Naoki Hisamoto, Anindya Ghosh-Roy, Sandhya P. Koushika
Summary: The study demonstrates that UNC-16 inhibits axonal regeneration by limiting the availability of the long isoform of DLK-1 at the injury site and regulating cytoskeletal dynamics. Additionally, UNC-16's inhibitory role in regeneration occurs through a tight temporal and spatial control of DLK-1 and cytoskeletal dynamics, ultimately affecting the functional recovery in unc-16 mutants.
Review
Cell Biology
Ana Catarina Costa, Monica Mendes Sousa
Summary: Neurons are polarized cells and the regulation of the microtubule cytoskeleton is crucial for their function. A group of microtubule-severing enzymes, including spastin, have been found to play an important role in regulating microtubule organization and axonal transport.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Neurosciences
Tatsuhiro Shimizu, Kayoko Sugiura, Yoshiki Sakai, Abdul R. Dar, Rebecca A. Butcher, Kunihiro Matsumoto, Naoki Hisamoto
Summary: Chemical communication plays a crucial role in axon regeneration, and the ascaroside signaling system serves as a unique example of regulating the regenerative pathway in the nervous system.
JOURNAL OF NEUROSCIENCE
(2022)
Article
Medicine, Research & Experimental
Zhu Guo, Chengjun Li, Yong Cao, Tian Qin, Liyuan Jiang, Yan Xu, Miao Li, Zixiang Luo, Jianzhong Hu, Hongbin Lu
Summary: This study revealed that UTX regulates axonal regeneration and functional recovery post-SCI by modulating miR-24 and NeuroD1. Downregulation of UTX promotes axonal regeneration, suggesting UTX as a potential therapeutic target in SCI.
MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT
(2021)
Article
Neurosciences
Yoshiki Sakai, Mayuka Tsunekawa, Kohei Ohta, Tatsuhiro Shimizu, Strahil Iv. Pastuhov, Hiroshi Hanafusa, Naoki Hisamoto, Kunihiro Matsumoto
Summary: In Caenorhabditis elegans hermaphrodites, axon injury activates TLN-1/talin through the cAMP-Epac-Rap GTPase cascade, leading to integrin inside-out activation and subsequent activation of the RhoA-ROCK signaling pathway. The C. elegans integrin signaling network regulates axon regeneration through the Src-RhoGEF-RhoA axis.
JOURNAL OF NEUROSCIENCE
(2021)
Article
Neurosciences
Bekir Altas, Andrea J. Romanowski, Garrett W. Bunce, Alexandros Poulopoulos
Summary: The subcellular localization of mTOR in neurons has significant implications for nervous system development, function, and disease. The model of mTOR outposts suggests that localized signaling is selected and amplified through these structures.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Shingo Koinuma, Ryota Negishi, Riko Nomura, Kazuki Sato, Takuya Kojima, Eri Segi-Nishida, Ryo Goitsuka, Yoichiro Iwakura, Naoyuki Wada, Yoshiki Koriyama, Sumiko Kiryu-Seo, Hiroshi Kiyama, Takeshi Nakamura
Summary: Results suggest that TC10 plays a crucial role in axon regeneration within both peripheral and central nervous systems. Lack of TC10 significantly suppresses axon regeneration post-injury, while not affecting brain size and structure during development. TC10 exerts its effects on axon regeneration by stabilizing microtubules and acting as a neuron-intrinsic factor.
JOURNAL OF NEUROCHEMISTRY
(2021)
Article
Neurosciences
Yoshiki Sakai, Hiroshi Hanafusa, Tatsuhiro Shimizu, Strahil I. Pastuhov, Naoki Hisamoto, Kunihiro Matsumoto
Summary: This study reveals a novel role for BRCA1 and its partner BARD1 in neuronal cells, showing their involvement in regulating axon regeneration through the Gq alpha-DAG signaling network.
JOURNAL OF NEUROSCIENCE
(2021)
Article
Multidisciplinary Sciences
Valerie Van Steenbergen, Flavie Lavoie-Cardinal, Youcef Kazwiny, Marianna Decet, Tobie Martens, Patrik Verstreken, Werend Boesmans, Paul De Koninck, Pieter Vanden Berghe
Summary: The correct distribution of organelles and vesicles in cells is crucial for cell functioning. In polarized cells such as neurons, the transport of mitochondria, which play a key role in producing energy and buffering calcium, is especially important. This study found that the molecular conformation of tubulin affects mitochondrial transport in neurons. The position of a mitochondrion within or at the rim of a microtubule bundle determines the impact of tubulin conformation on mitochondrial transport.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Jingwen Niu, Sabrina M. Holland, Andrea Ketschek, Kaitlin M. Collura, Natasha L. Hesketh, Takashi Hayashi, Gianluca Gallo, Gareth M. Thomas
Summary: This article investigates the relationship between dual leucine-zipper kinase (DLK) and the neural-specific isoform JNK3, and finds that JNK3 activates DLK by positive feedback phosphorylation and locks it in a highly active state. Unlike the JNK1 isoform, JNK2 and JNK3 promote prodegenerative signaling. Additionally, palmitoylation plays an important role in the axonal localization of DLK and JNK3, as well as in axonal retrograde signaling in response to optic nerve injury.
Article
Neurosciences
Naoki Hisamoto, Yoshiki Sakai, Kohei Ohta, Tatsuhiro Shimizu, Chun Li, Hiroshi Hanafusa, Kunihiro Matsumoto
Summary: This study reveals that CDK14 activates the RhoGEF-CDC42-MLC phosphorylation axis in a noncanonical Wnt signaling pathway to promote axon regeneration in Caenorhabditis elegans. The results suggest a novel regulatory mechanism for neuronal regeneration and provide new insights into the molecular mechanisms underlying axon regeneration.
JOURNAL OF NEUROSCIENCE
(2021)
Article
Cell Biology
Jianli Hu, William Rodemer, Guixin Zhang, Li-Qing Jin, Shuxin Li, Michael E. Selzer
Summary: Paralysis following spinal cord injury is caused by failed axonal regeneration, which may be inhibited by inhibitory molecules like CSPGs. ChABC treatment can enhance axon growth and reduce retrograde neuronal apoptosis signaling, possibly through regulating PTP sigma expression and Akt activation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Neurosciences
Jose Ricardo Vieira, Bhavin Shah, Sebastian Dupraz, Isidora Paredes, Patricia Himmels, Geza Schermann, Heike Adler, Alessia Motta, Lea Gaertner, Ariadna Navarro-Aragall, Elena Ioannou, Elena Dyukova, Remy Bonnavion, Andreas Fischer, Dario Bonanomi, Frank Bradke, Christiana Ruhrberg, Carmen Ruiz de Almodovar
Summary: This study reveals the importance of communication between motor neurons and endothelial cells in spinal cord development, and the role of semaphorin 3C and PlexinD1 in regulating this process.
Article
Cell Biology
Li-Qing Jin, Yan Zhou, Yue-Sheng Li, Guixin Zhang, Jianli Hu, Michael E. Selzer
Summary: Axotomy in the CNS can lead to either axon regeneration or cell death depending on retrograde signals. Local protein synthesis in axon tips plays a crucial role in axon retraction and regeneration. Single-cell RNA-seq analysis reveals that differentially regulated MAPK and circadian rhythm signaling pathways may be involved in activating different programs for axon regeneration or retraction and apoptosis.
Review
Cell Biology
Yu-Chun Wu, Yueh-Chen Chiang, Shih-Hua Chou, Chun-Liang Pan
Summary: Wnt signalling pathway regulates development and morphogenesis in metazoans through endocytosis, with recent studies suggesting its involvement in cellular stress responses in adulthood. This review summarizes the molecular and cellular mechanisms of how endocytosis modulates Wnt signalling and discusses its potential repurposing to regulate mitochondrial stress response in the nematode C. elegans.
BIOLOGY OF THE CELL
(2021)
Article
Cell Biology
Li-Tzu Chen, Chih-Ta Lin, Liang-Yi Lin, Jiun-Min Hsu, Yu-Chun Wu, Chun-Liang Pan
Summary: In C. elegans, loss of neuronal fzo-1/mito-fusin induces nonautonomous UPRmt through multiple neurotransmitters and neurohormones. This triggers nonautonomous mitochondrial fragmentation, requiring genes involved in autophagy and mitophagy. The systemic activation of UPRmt and mitochondrial fragmentation improves resistance to pathogenic Pseudomonas infection in C. elegans.
DEVELOPMENTAL CELL
(2021)
Article
Biochemistry & Molecular Biology
Shan-Shan Lin, You-An Su, Mei-Chun Chuang, Ya-Wen Liu
Summary: Invadosomes are critical actin structures for cell migration, invasion, and extracellular matrix remodeling, with their dynamic, proteolytic, and protrusive nature attracting scientists to develop new technologies for analysis. Through exciting methodologies, researchers have well characterized the biochemical and biophysical properties of invadosomes, elegantly explaining their biological and pathological effects on human health and diseases. The opposite regulatory mechanisms between invadosomes and focal adhesions have been explored, with speculation on a potential rule for their switching mechanism.
Article
Neurosciences
Shih-Hua Chou, Yen-Ju Chen, Chien-Po Liao, Chun-Liang Pan
Summary: Physiological stress triggers aversive learning in animals, and mitochondrial disruption regulates dopamine signaling to affect the learned bacterial avoidance behavior of C. elegans.
NEUROSCIENCE RESEARCH
(2022)
Article
Multidisciplinary Sciences
Albert A. Lee, William Y. C. Huang, Scott D. Hansen, Neil H. Kim, Steven Alvarez, Jay T. Groves
Summary: The study reveals that the catalytic rate of interfacial enzymes can be strongly influenced by the size of the reaction system, and stochastic effects originating from low molecular copy numbers can alter reaction kinetics. The final product of competitive enzymatic cycles depends on the size of the reaction system, and this dependency can be controlled by engineering feedback mechanisms into the enzymes.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Yueh-Chen Chiang, Chien-Po Liao, Chun-Liang Pan
Summary: Mitochondrial disruption in nonneural tissues of Caenorhabditis elegans induces learned aversion for nutritious bacterial food, involving the regulation of neurotransmitters such as serotonin. In addition, calcium imaging experiments reveal altered neural responses to bacterial cues in a fraction of stress-primed animals.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Materials Science, Ceramics
Chunliang Pan, Lingling Xie, Tao Zhou, XinXin Yin, Yu Niu, Jing Xu, Qing Han, Xinli Yang, Limin Zhu, Xiaoyu Cao
Summary: In this work, nitrogen-doped carbon-coated NaV3O8 materials were successfully synthesized using a rheological phase method with melamine as a nitrogen source. The nitrogen-doped carbon coating improved the thickness and properties of the surface coating without changing the crystal structure of NVO. The best electrochemical properties were observed in NVO@NC with a molar ratio of melamine to citric acid of 2.75:1.25. The nitrogen-doped carbon coating enhanced the discharge capacity and sodium ion diffusion coefficient, providing NVO materials with high-capacity and ultrafast Na-ion storage.
CERAMICS INTERNATIONAL
(2022)
Article
Developmental Biology
Chung-Kuan Chen, Chun-Liang Pan
Summary: This review summarizes recent advances and unsolved issues in cell polarity control by Wnt glycoproteins, focusing on their involvement in cytoskeletal mechanisms or transcriptional mechanisms. The importance of using the nematode Caenorhabditis elegans as a model organism to validate the role of polarity-inducing factors in vivo is emphasized.
DEVELOPMENTAL BIOLOGY
(2022)
Editorial Material
Cell Biology
Chun-Liang Pan
Summary: The study shows that mechanosensory ion channels play a crucial role in mediating the effects of cell membrane guidance cues on the morphogenesis of neuronal dendrites.
DEVELOPMENTAL CELL
(2022)
Article
Biology
Scott D. Hansen, Albert A. Lee, Benjamin R. Duewell, Jay T. Groves
Summary: The lipid-modifying enzyme PIP5K is essential for generating PI(4,5)P-2 lipids in eukaryotic cells. It is regulated by binding to these lipids and membrane-mediated dimerization, which enhances its catalytic efficiency and stochastic variation in kinase reaction velocity.
Article
Engineering, Electrical & Electronic
Chunliang Pan, Yuning Pan
Summary: This study combines RBF neural network algorithm and dynamic weight-based NSC method to improve the effect of fractal art pattern design. The study shows the changing rules of competition relationship and the distribution of competitors under noise interference, and proposes a method to control the style of art pattern design in the spatial domain. Experimental results demonstrate that the CAD fractal art pattern design system proposed in this study has a good effect.
ADVANCES IN MULTIMEDIA
(2022)
Article
Biochemistry & Molecular Biology
Chien-Po Liao, Yueh-Chen Chiang, Wai Hou Tam, Yen-Ju Chen, Shih-Hua Chou, Chun-Liang Pan
Summary: Physiological stress induces aversive memory formation through neural circuits and neuromodulatory mechanisms.
Article
Neurosciences
Yen-Ju Chen, Chun-Liang Pan
Summary: In this study, a sensory-interneuron circuit for mitochondrial stress-induced avoidance behavior in C. elegans was characterized. Olfactory sensory neurons and the AIY interneuron were found to be essential, with olfactory neurons acting upstream of AIY. Unlike pathogen avoidance, stress-induced avoidance did not require AIB, AIZ, or RIA interneurons. Ablation or inhibition of the head motor neurons SMDD/V altered worm locomotion and reduced avoidance. These findings contribute to our understanding of the neural circuit mechanisms underlying learned avoidance behavior triggered by mitochondrial stress.
NEUROSCIENCE RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Jen-Wei Weng, Heenam Park, Claire Valotteau, Rui-Tsung Chen, Clara L. Essmann, Nathalie Pujol, Paul W. Sternberg, Chun-Hao Chen
Summary: The study reveals that C. elegans males recognize conspecific and reproductive mates through mechanical cues, specifically through a group of cuticular collagens that maintain body stiffness. The findings highlight the importance of mechanosensory cues in mate recognition.
Article
Biochemical Research Methods
Chun-Hao Chen, Chun-Liang Pan
Summary: This protocol outlines how to use spinning-disk confocal microscopy to perform live-cell imaging of migrating PVD dendritic growth cones in C. elegans, allowing continuous observation for 4-6 hours and revealing dynamic molecular and cellular events in dendrite and axon development.
