期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 111, 期 21, 页码 7659-7664出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1322248111
关键词
RNA structure; RNA catalysis; GIR1; crystallography; SAXS
资金
- Centre National de la Recherche Scientifique
- University of Strasbourg
- L'Agence Nationale de la Recherche [ANR-10-BLAN-1502-02]
- le Laboratoire d'Excellence Project [ANR-11-LABX-0057-MITOCROSS]
- Danish Council for Independent Research in Natural Sciences
- Agence Nationale de la Recherche (ANR) [ANR-10-BLAN-1502] Funding Source: Agence Nationale de la Recherche (ANR)
The lariat-capping (LC) ribozyme is a natural ribozyme isolated from eukaryotic microorganisms. Despite apparent structural similarity to group I introns, the LC ribozyme catalyzes cleavage by a 2',5' branching reaction, leaving the 3' product with a 3-nt lariat cap that functionally substitutes for a conventional mRNA cap in the downstream pre-mRNA encoding a homing endonuclease. We describe the crystal structures of the precleavage and postcleavage LC ribozymes, which suggest that structural features inherited from group I ribozymes have undergone speciation due to profound changes in molecular selection pressure, ultimately giving rise to an original branching ribozyme family. The structures elucidate the role of key elements that regulate the activity of the LC ribozyme by conformational switching and suggest a mechanism by which the signal for branching is transmitted to the catalytic core. The structures also show how conserved interactions twist residues, forming the lariat to join chemical groups involved in branching.
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