Article
Multidisciplinary Sciences
Leo Shen, Lauren Pugsley, Regina Cencic, HanChen Wang, Francis Robert, Sai Kiran Naineni, Ananya Sahni, Genevieve Morin, Wenhan Zhang, Anastasia Nijnik, John A. Porco, David Langlais, Sidong Huang, Jerry Pelletier
Summary: The article discusses the mechanism of action and resistance mechanisms of eukaryotic translation initiation inhibitors rocaglates, identifying transcription factors FOXP3 and NR1I3 as potent modifiers of rocaglate response. Additionally, FOXP3 and NR1I3 confer a multi-drug resistant phenotype that can be reversed by inhibiting Pgp, suggesting a potential therapeutic combination strategy.
SCIENTIFIC REPORTS
(2021)
Article
Plant Sciences
Yuan Lin, Hainan Zhao, Magdalena Kotlarz, Jiming Jiang
Summary: Gene expression is regulated by interactions between cis-regulatory DNA elements (CREs) and regulatory proteins. Enhancers play a crucial role in this process, especially in eukaryotes, but little is known about the molecular mechanisms involved in plants. Researchers identified a distal enhancer, CRE beta, which regulates gene expression in response to osmotic pressure, and established a forward genetic screen pipeline to identify trans-regulatory factors associated with CRE beta function and the regulation of the endogenous gene AtDGK7.
Review
Oncology
Akos Gasparics, Attila Sebe
Summary: Forward genetic screens have been instrumental in identifying oncogenes, tumor suppressor genes, and genes related to metastasis and therapy resistance. EMT, regulated by complex signaling networks, plays a crucial role in chronic fibroproliferative diseases and cancer. Investigating the mechanisms leading to EMT and understanding its role in cancer is essential for therapeutic interventions.
Article
Behavioral Sciences
Eva Sheardown, Aleksandra M. Mech, Maria Elena Miletto Petrazzini, Adele Leggieri, Agnieszka Gidziela, Saeedeh Hosseinian, Ian M. Sealy, Jose Torres-Perez, Elisabeth M. Busch-Nentwich, Margherita Malanchini, Caroline H. Brennan
Summary: Psychiatric disorders are a significant burden in society, but the genetic factors contributing to their etiology are still poorly understood. Animal models and genomic studies in human populations can provide valuable insights into the causes of psychiatric diseases.
NEUROSCIENCE AND BIOBEHAVIORAL REVIEWS
(2022)
Article
Plant Sciences
Shaogan Wang, Stefan Robertz, Merve Seven, Florian Kraemer, Benjamin M. Kuhn, Lifeng Liu, China Lunde, Markus Pauly, Vicente Ramirez
Summary: Through chemical mutagenesis of maize, a collection of mutants with diverse alterations in cell wall attributes were identified. Some mutants showed increased saccharification yield, while others showed decreased yield. These mutants, called candy-leaf (cal), provide a valuable tool to understand the recalcitrance of grass lignocellulosics and grass-specific aspects of cell wall biology.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Oncology
Manuel C. Scheidmann, Francesc Castro-Giner, Karin Strittmatter, Ilona Krol, Aino Paasinen-Sohns, Ramona Scherrer, Cinzia Donato, Sofia Gkountela, Barbara M. Szczerba, Zoi Diamantopoulou, Simone Muenst, Tatjana Vlajnic, Leo Kunz, Marcus Vetter, Christoph Rochlitz, Verdon Taylor, Claudio Giachino, Timm Schroeder, Randall J. Platt, Nicola Aceto
Summary: This study identified specific genetic dependencies for each step of the metastatic process in breast cancer using a newly derived CTC xenograft model and a loss-of-function CRISPR screen. The findings provide insights into the biology of metastasis and potential targeted treatment approaches for metastatic breast cancer.
Article
Biology
Yonghe Ding, Di Lang, Jianhua Yan, Haisong Bu, Hongsong Li, Kunli Jiao, Jingchun Yang, Haibo Ni, Stefano Morotti, Tai Le, Karl J. Clark, Jenna Port, Stephen C. Ekker, Hung Cao, Yuji Zhang, Jun Wang, Eleonora Grandi, Zhiqiang Li, Yongyong Shi, Yigang Li, Alexey Glukhov, Xiaolei Xu
Summary: The study utilized a gene-break transposon (GBT) library to identify a novel causative gene Dnajb6 for sick sinus syndrome, revealing the heterogeneity of cardiac pacemaker cells.
Article
Plant Sciences
Karen Hudson
Summary: Mutagenesis is an important tool in soybean biology, used to create stable non-transgenic variations. A genetic screen resulted in the identification of over 100 mutant lines with aberrant oil or protein composition traits, particularly focusing on high protein or high oil phenotypes. These lines were selected and followed for multiple seasons and generations for further characterization.
Article
Genetics & Heredity
David R. Smyth
Summary: In the late 1980s, geneticists began using chemical mutagens to induce random mutations in seeds and screen for plants with defective flower development. The study conducted at Caltech and Monash University focused on Arabidopsis thaliana mutants and identified genes controlling flower organ identity, meristem identity, organ development, and meristem properties. These findings have contributed to our understanding of transcriptional control, signaling, and the role of auxin in initiating flower development.
Article
Oncology
Holger Weishaupt, Matko Cancer, Gabriela Rosen, Karl O. Holmberg, Susana Haggqvist, Ignas Bunikis, Yiwen Jiang, Smitha Sreedharan, Ulf Gyllensten, Oren J. Becher, Lene Uhrbom, Adam Ameur, Fredrik J. Swartling
Summary: This study demonstrates a novel approach to tagging glioma genes using retroviral integration sites. By using a high-throughput pipeline coupled to a tumor model system, the researchers were able to detect retroviral integration sites in malignant stem cell lines from glioma biopsies. The results identified genes that have been dysregulated in glioma and also discovered novel potential cancer-causing genes.
Article
Biology
Jennette M. Codjoe, Ryan A. Richardson, Fionn McLoughlin, Richard David Vierstra, Elizabeth S. Haswell
Summary: This study reveals that the Arabidopsis MSL10 channel is associated and functions with proteins at endoplasmic reticulum-plasma membrane contact sites (EPCSs). Mutations in the synaptotagmin genes were identified as suppressors of MSL10 gain-of-function phenotype. In addition, the expansion of EPCSs in msl10-3G plants provides new insights into the function and regulation of this subcellular compartment.
Article
Cell Biology
Ryan H. Moy, Alexander Nguyen, Jia Min Loo, Norihiro Yamaguchi, Christina M. Kajba, Balaji Santhanam, Benjamin N. Ostendorf, Y. Gloria Wu, Saeed Tavazoie, Sohail F. Tavazoie
Summary: Metastatic colonization is a major cause of death from colorectal cancer. This study identified 26 gene promoters that promote liver colonization in colorectal cancer. Among these genes, ITPR3 was found to promote liver-metastatic colonization and induce similar downstream gene expression programs. The study also revealed that ITPR3 and RELB drive CRC colony formation by promoting cell survival upon substratum detachment or hypoxic exposure. The findings implicate the ITPR3/calcium/RELB axis in CRC metastatic colony formation.
DEVELOPMENTAL CELL
(2022)
Article
Biochemistry & Molecular Biology
Yan Xu, Kevin Ao, Lei Tian, Yilan Qiu, Xingchuan Huang, Xueru Liu, Ryan Hoy, Yishan Zhang, Khalid Youssef Rashid, Shitou Xia, Xin Li
Summary: This study introduces a new research method that combines genetic screening and high-throughput sequencing to quickly discover key genes in plant fungal pathogens. The research found that melanin is crucial for the survival, structure, and reproduction of sclerotia, demonstrating the potential application value of the new method in fungal pathogen research.
MOLECULAR PLANT-MICROBE INTERACTIONS
(2022)
Article
Genetics & Heredity
Joy H. Meserve, Jessica C. Nelson, Kurt C. Marsden, Jerry Hsu, Fabio A. Echeverry, Roshan A. Jain, Marc A. Wolman, Alberto E. Pereda, Michael Granato
Summary: The acoustic startle response is an evolutionarily conserved avoidance behavior that can be disrupted in human neurological disorders. Using zebrafish larvae, a genetic screen identified eight genes critical for regulating startle behavior, with two of the genes associated with human disorders. Experimental validation showed that these genes play key roles in regulating movement magnitude in the spinal cord.
Article
Biochemistry & Molecular Biology
Marie B. Cordon, Kristian M. Jacobsen, Cecilie S. Nielsen, Per Hjerrild, Thomas B. Poulsen
Summary: A phenotypic screen identified compounds that selectively target liver cancer cells and induce ferroptotic cell death, which can also occur under hypoxic conditions. Further studies revealed reduced sensitivity of some compounds under hypoxic conditions, suggesting potential implications for the use of ferroptosis-inducers as anti-cancer agents.
Article
Biochemistry & Molecular Biology
Hua Gao, Goutam Chakraborty, Zhanguo Zhang, Intissar Akalay, Mayur Gadiya, Yaquan Gao, Surajit Sinha, Jian Hu, Cizhong Jiang, Muzaffar Akram, Edi Brogi, Birgit Leitinger, Filippo G. Giancotti
Article
Biochemistry & Molecular Biology
Hua Gao, Goutam Chakraborty, Ai Ping Lee-Lim, Qianxing Mo, Markus Decker, Alin Vonica, Ronglai Shen, Edi Brogi, Ali H. Brivanlou, Filippo G. Giancotti
Article
Biochemistry & Molecular Biology
H Gao, Y Sun, YL Wu, B Luan, YY Wang, B Qu, G Pei
Article
Biochemistry & Molecular Biology
P Wang, H Gao, YX Ni, BB Wang, YL Wu, LL Ji, LH Qin, L Ma, G Pei
JOURNAL OF BIOLOGICAL CHEMISTRY
(2003)