Article
Chemistry, Analytical
Lei Wen, Xinyu Ma, Jing Yang, Minmin Jiang, Chao Peng, Zhongyun Ma, Huan Yu, Yinhui Li
Summary: This study proposed a new design strategy based on pi-conjugation modulation for fluorescent probes, which can generate different emission bands in the presence and absence of the target. The newly designed fluorescence probe, Rhod-DCM-B, exhibited outstanding sensitivity and excellent selectivity towards ONOO-. It was successfully employed to determine endogenous and exogenous ONOO- in living cells, and effectively monitored the ONOO- in vivo.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Stanislava Vrankova, Zuzana Galandakova, Jakub Benko, Martina Cebova, Igor Riecansky, Olga Pechanova
Summary: The results of the study indicate that 10 weeks of social isolation led to a significant decrease in PPI, accompanied by a decrease in NOS activity in the cerebral cortex and the cerebellum, an increase in iNOS in the hippocampus, and an increase in CD concentration in cortex homogenate. On the other hand, 29 weeks of social isolation had an opposite effect on NOS activity, with an increase in the cerebral cortex and the cerebellum in animals reared in a social environment, accompanied by a decrease in CD concentration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Xiaoxiao Liu, Di Gong, Qingbo Ke, Lina Yin, Shiwen Wang, Tianpeng Gao
Summary: A meta-analysis of 86 studies has shown that nitric oxide (NO) can significantly improve plant biomass, alleviate oxidative damage, and increase the content of Ca, Fe, and Mg in heavy metal-stressed plants. The optimal concentration of SNP (an NO donor) for hydroponic culture is in the range of 75-150 μM. These findings provide valuable insights for the future application of NO as a plant growth regulator for enhancing heavy metal stress tolerance in agriculture and breeding.
Review
Food Science & Technology
Joseph Festa, Mariasole Da Boit, Aamir Hussain, Harprit Singh
Summary: Cardiovascular disease is the leading cause of global death, with endothelial dysfunction being a predictor for most CVD. Polyphenols from sources like wine and grape extracts can help prevent endothelial dysfunction, while anthocyanin flavonoids found in berries also improve vascular function.
MOLECULAR NUTRITION & FOOD RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Thuan Thai, Fei Zhong, Lei Dang, Enoch Chan, Jacqueline Ku, Ernst Malle, Carolyn L. Geczy, John F. Keaney, Shane R. Thomas
Summary: The study demonstrates that transcytosed MPO in endothelial cells activates eNOS by promoting phospholipase C-dependent calcium signaling and altering eNOS phosphorylation status. This activation occurs despite reduced cellular uptake of the eNOS substrate L-arginine. These findings suggest a compensatory signaling response in endothelial cells to maintain eNOS activity and NO production in the presence of MPO-induced oxidative stress.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Ophthalmology
Tadanobu Yoshikawa, Kenji Obayashi, Kimie Miyata, Keigo Saeki, Nahoko Ogata
Summary: The study revealed a significant association between higher serum ADMA levels and severe glaucoma, which remained significant after adjusting for potential confounders.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2021)
Review
Biochemistry & Molecular Biology
Yuting Meng, Huaikang Jing, Jing Huang, Renfang Shen, Xiaofang Zhu
Summary: Nitric oxide (NO) is a crucial signaling molecule in plants, playing roles in regulating plant growth and development, and responding to stress. Cadmium (Cd) pollution is a widespread environmental hazard that inhibits plant growth and threatens human health. Plants have evolved mechanisms to cope with Cd stress, and NO is known to be involved in these responses. However, the mechanisms by which Cd stress affects the accumulation of endogenous NO in plants and the role of exogenous NO in plant responses to Cd stress remain unclear.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Xilei Xie, Yawen Liu, Guangzhao Liu, Yuying Zhao, Jinyan Liu, Yong Li, Jian Zhang, Xiaoyun Jiao, Xu Wang, Bo Tang
Summary: In this study, a novel fluorescent probe was designed and synthesized, allowing the visualization of the crucial role of peroxynitrite in Alzheimer's disease through in vivo experiments in mice.
CHEMICAL COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Renato C. Nogueira, Magdalena Minnion, Anna D. Clark, Alex Dyson, Jose E. Tanus-Santos, Martin Feelisch
Summary: Nitrite concentrations in blood play a crucial role in assessing intracellular nitric oxide (NO) activity and endothelial function. However, using nitrosylhemoglobin (HbNO) as an early marker of COVID-19 patient risk may be premature.
Article
Biochemistry & Molecular Biology
Oriana Simonetti, Tiziana Bacchetti, Gianna Ferretti, Elisa Molinelli, Giulio Rizzetto, Luisa Bellachioma, Annamaria Offidani
Summary: The study revealed that children with atopic dermatitis (AD) have higher levels of lipid peroxidation and lower antioxidant defense, with abnormal activity of PON enzymes, indicating a relationship between oxidative stress and inflammation in AD patients.
Review
Biochemistry & Molecular Biology
Juan A. Godoy, Juvenal A. Rios, Pol Picon-Pages, Victor Herrera-Fernandez, Bronte Swaby, Giulia Crepin, Ruben Vicente, Jose M. Fernandez-Fernandez, Francisco J. Munoz
Summary: Mitochondria play critical roles in neurons by regulating neurotransmission and synaptic plasticity through ATP supply, calcium homeostasis, redox balance control, and apoptosis. Their functional integrity is mainly maintained by mitostasis, involving processes like mitochondrial transport, anchoring, fusion, and fission, regulated by various signaling pathways. The communication between mitochondria and the endoplasmic reticulum (ER) via mitochondria-associated membranes (MAMs) is crucial for Ca2+ buffering. Alterations in mitochondrial activity can lead to increased production of reactive oxygen species, disturbance in physiological metabolism, and mitochondrial dysfunction resulting in cell death through apoptosis.
Article
Plant Sciences
Cheng Qin, Jie Shen, Mohammad Abass Ahanger
Summary: Experiments were conducted to evaluate the role of exogenously applied nitric oxide (NO) and spermidine (Spd) in alleviating the damaging effects of nickel toxicity on tomato. It was found that the application of NO and Spd effectively reduced the harmful effects of nickel, improving the growth and physiological parameters of tomato plants.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Elena Grossini, Divya Garhwal, Sakthipriyan Venkatesan, Daniela Ferrante, Angelica Mele, Massimo Saraceno, Ada Scognamiglio, Jessica Mandrioli, Amedeo Amedei, Fabiola De Marchi, Letizia Mazzini
Summary: This study analyzed the plasma redox system and nitric oxide levels in ALS patients and investigated the effects of plasma on mitochondrial function in endothelial vascular cells and astrocytes. The results showed that oxidative stress and neurovascular unit changes play a central role in ALS, and unknown plasma factors may be involved in the disease pathogenesis.
Article
Nutrition & Dietetics
Agnieszka Zembron-Lacny, Edyta Wawrzyniak-Gramacka, Anna Ksiazek, Aleksandra Zagrodna, Wieslaw Kopec, Malgorzata Slowinska-Lisowska
Summary: Intense physical exercise leads to increased production of reactive oxygen and nitrogen species. This study examined the effects of dipeptide intake combined with exercise on the oxi-antioxidant response. The results showed that dipeptide intake led to a significant increase in the NO/H2O2 ratio after exercise, indicating enhanced antioxidant status. Additionally, dipeptides were found to reduce C-reactive protein levels, suggesting their anti-inflammatory properties. These findings suggest that dipeptides have potential in reducing oxidative stress and inflammation in physically active individuals.
Article
Nutrition & Dietetics
Gaizun Hu, Lusi Xu, Osamu Ito
Summary: Chronic exercise has antihypertensive and renoprotective effects in rats on a high fructose diet. The study reveals that the high fructose diet affects the nitric oxide (NO) system and oxidative stress in the kidney, while exercise improves these effects.
Article
Toxicology
Simone Rothmiller, Niklas Jaeger, Nicole Meier, Thimo Meyer, Adrian Neu, Dirk Steinritz, Horst Thiermann, Michael Scherer, Christoph Rummel, Aswin Mangerich, Alexander Buerkle, Annette Schmidt
Summary: The study indicates that exposure to sulfur mustard (SM) may lead to chronic senescent mesenchymal stem cells (MSCs) which can affect wound healing. Selective removal of these cells opens the possibility for an innovative treatment strategy.
ARCHIVES OF TOXICOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Bernhard Luscher, Ivan Ahel, Matthias Altmeyer, Alan Ashworth, Peter Bai, Paul Chang, Michael Cohen, Daniela Corda, Francoise Dantzer, Matthew D. Daugherty, Ted M. Dawson, Valina L. Dawson, Sebastian Deindl, Anthony R. Fehr, Karla L. H. Feijs, Dmitri V. Filippov, Jean-Philippe Gagne, Giovanna Grimaldi, Sebastian Guettler, Nicolas C. Hoch, Michael O. Hottiger, Patricia Korn, W. Lee Kraus, Andreas Ladurner, Lari Lehtio, Anthony K. L. Leung, Christopher J. Lord, Aswin Mangerich, Ivan Matic, Jason Matthews, George-Lucian Moldovan, Joel Moss, Gioacchino Natoli, Michael L. Nielsen, Mario Niepel, Friedrich Nolte, John Pascal, Bryce M. Paschal, Krzysztof Pawlowski, Guy G. Poirier, Susan Smith, Gyula Timinszky, Zhao-Qi Wang, Jose Yelamos, Xiaochun Yu, Roko Zaja, Mathias Ziegler
Summary: ADP-ribosylation, a post-translational modification of proteins, nucleic acids, and metabolites, plays diverse roles in cellular processes such as stress responses, signaling, and transcriptional regulation. Recent advances in research have identified a wide range of cellular pathways regulated by ADP-ribosylation, highlighting the importance of understanding this mechanism in cell biology.
Letter
Toxicology
M. Batke, G. Damm, H. Foth, A. Freyberger, T. Gebel, U. Gundert-Remy, J. Hengstler, A. Mangerich, F. Partosch, C. Roehl, T. Schupp, K. M. Wollin
ARCHIVES OF TOXICOLOGY
(2022)
Article
Toxicology
C. Roehl, M. Batke, G. Damm, A. Freyberger, T. Gebel, U. Gundert-Remy, J. G. Hengstler, A. Mangerich, A. Matthiessen, F. Partosch, T. Schupp, K. M. Wollin, H. Foth
Summary: Bromate, a by-product of water disinfection, was found to have high concentrations in swimming pools. It was determined to be a non-threshold carcinogen based on its ability to induce DNA damage. Different age and activity groups showed varying exposure levels, with top athletes having the highest exposure and adults having the lowest exposure.
ARCHIVES OF TOXICOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Joanna A. Ruszkiewicz, Alexander Buerkle, Aswin Mangerich
Summary: In this article, the crucial role of NAD(+) in mechanisms promoting genome maintenance is reviewed, including genome stability protection, DNA damage response, and nucleic acid metabolism regulation. The challenges associated with therapeutic actions aimed at increasing NAD(+) levels are also discussed.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Cell Biology
Julia M. Reber, Jovana Bozic-Petkovic, Michelle Lippmann, Marvin Mazzardo, Asisa Dilger, Rebecca Warmers, Alexander Buerkle, Aswin Mangerich
Summary: PARP1 and XRCC1 have important reciprocal regulatory functions during genotoxic stress response, and the recruitment of one is impeded in the absence of the other. XRCC1 KO cells exhibit a hypersensitivity phenotype compared to PARP1 KO cells, which can be rescued by additional genetic deletion of PARP1 or pharmacological PARP inhibition.
CELL BIOLOGY AND TOXICOLOGY
(2023)
Article
Microbiology
Taylor A. Dodson, Eric A. Carlson, Nathan C. Wamer, Chase N. Morse, Jennifer N. Gadient, Erin G. Prestwich
Summary: Pseudomonas aeruginosa biofilms can cause chronic infections and are difficult to eradicate due to their complexity. This study identified three cell subpopulations in P. aeruginosa biofilms, each with distinct morphologies, phenotypes, and biochemistries. Understanding the diversity of bacterial cells in biofilms can help in developing more effective treatments.
Article
Cell Biology
Konstantin N. Naumenko, Mariya V. Sukhanova, Loic Hamon, Tatyana A. Kurgina, Rashid O. Anarbaev, Aswin Mangerich, David Pastre, Olga I. Lavrik
Summary: Y-box-binding protein 1 (YB-1) is involved in the regulation of gene expression and has been found to play a role in the regulation of PARP1 activity. The C-terminal domain of YB-1 is able to interact with PAR and control its synthesis, providing important insights into the regulation of PARP1 activity.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemical Research Methods
Nathan C. Wamer, Chase N. Morse, Jennifer N. Gadient, Taylor A. Dodson, Eric A. Carlson, Erin G. Prestwich
Summary: Different bacterial cell surface associated biomolecules can be identified by using matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry and collision induced dissociation (CID) technique. Pseudomonas aeruginosa, an opportunistic Gram-negative bacterium, communicates through quorum sensing (QS) system, which produces biosurfactant rhamnolipids that alter cellular membrane. We investigated six common matrices and observed selective ionization of three major molecule classes (quinolones, rhamnolipids, and phospholipids) in P. aeruginosa. The matrix selection plays an important role in MALDI-TOF identification of molecules in complex biological mixtures.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2023)
Article
Medicine, Research & Experimental
Taylor A. Dodson, Stephan Nieuwoudt, Chase N. Morse, Valinteshley Pierre, Chao Liu, Samuel E. Senyo, Erin G. Prestwich
Summary: We evaluated tRNA modifications in the diabetic heart and found that tRNA modifications play an important role in diabetic etiologies. We quantified tRNA modifications and ribonucleoside levels in healthy and diabetic murine hearts and hyperglycemic cell models. Results showed abnormal ribonucleoside modifications in hyperglycemic models and diabetic tissues. Our findings suggest that tRNA modifications may regulate translation in diabetes.
Article
Biochemistry & Molecular Biology
Tobias Loeffler, Annika Krueger, Peyman Zirak, Martin J. Winterhalder, Anna-Lena Mueller, Arthur Fischbach, Aswin Mangerich, Andreas Zumbusch
Summary: This study presents a targeted approach based on fluorescence correlation spectroscopy (FCS) to characterize the interactions between PAR molecules and three PAR-binding proteins. The results show that these interactions are structure-specific and dependent on the chain length of PAR. Rating: 8/10.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Biochemical Research Methods
Radhika Thanvi, Shadia Nada, Ravindika Dissanayake, Abhishek Vartak, Chloe Olayinka Sebilleau, Nur-E Alom, Erin G. Prestwich, Katherine A. Wall, Steven J. Sucheck
Summary: A prototype vaccine was developed using linear B-cell epitopes derived from OprF. The vaccine generated antibodies that bound to PA14 and induced immune responses in mice, suggesting its potential for protection against Pseudomonas aeruginosa infection.
BIOCONJUGATE CHEMISTRY
(2023)
Meeting Abstract
Pharmacology & Pharmacy
J. Ruszkiewicz, Y. Papatheodorou, J. Melzig, L. Kehrt, M. Thomann, A. Schmidt, S. Rothmiller, A. Buerkle, A. Mangerich
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
(2022)
Meeting Abstract
Pharmacology & Pharmacy
J. Ruszkiewicz, N. Hettich, E. Lorse, J. Huwer, A. Mangerich
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
(2022)
Meeting Abstract
Pharmacology & Pharmacy
H. Bastek, G. Mucic, T. Zubel, A. Mangerich, S. Beneke, D. Dietrich
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
(2022)