Article
Multidisciplinary Sciences
Cornelis J. J. Boogerd, Ilaria Perini, Eirini Kyriakopoulou, Su Ji Han, Phit La, Britt van der Swaan, Jari B. B. Berkhout, Danielle Versteeg, Jantine Monshouwer-Kloots, Eva van Rooij
Summary: The inability of adult human cardiomyocytes to proliferate is an obstacle to efficient cardiac regeneration after injury. Understanding the mechanisms that drive postnatal cardiomyocytes to switch to a non-regenerative state is therefore of great significance. Arid1a is found to suppress postnatal cardiomyocyte proliferation while enhancing maturation by promoting DNA access to transcription factors.
NATURE COMMUNICATIONS
(2023)
Article
Cardiac & Cardiovascular Systems
Huairui Shi, Yang Gao, Zhen Dong, Ji'e Yang, Rifeng Gao, Xiao Li, Shuqi Zhang, Leilei Ma, Xiaolei Sun, Zeng Wang, Feng Zhang, Kai Hu, Aijun Sun, Junbo Ge
Summary: The study demonstrates the significant role of GSDMD-mediated cardiomyocyte pyroptosis in myocardial I/R injury, and suggests that the caspase-11/GSDMD pathway may be essential to this process. Furthermore, GSDMD inhibition significantly reduces cardiomyocyte pyroptosis and I/R-induced myocardial injury.
CIRCULATION RESEARCH
(2021)
Article
Cardiac & Cardiovascular Systems
Ningning Guo, Di Zheng, Jiaxin Sun, Jian Lv, Shun Wang, Yu Fang, Zhenyi Zhao, Sai Zeng, Qiuxiao Guo, Jingjing Tong, Zhihua Wang
Summary: Nap1l5 plays a crucial role in regulating protein synthesis and hypertrophy in cardiomyocytes, and its overexpression exacerbates hypertrophic effects. Knockdown of Nap1l5 reverses the transcriptional reprogramming associated with hypertrophy, highlighting its potential as a target for therapeutic interventions in cardiac hypertrophy.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2021)
Article
Cell Biology
Yuhua Shi, Yang Yang, Weilv Xu, Dongyun Shi, Wei Xu, Xinyu Fu, Qian Lv, Jie Xia, Fushan Shi
Summary: This study reveals that pyroptosis triggered by Gasdermin D is regulated through ubiquitination. Specifically, SYVN1, an E3 ubiquitin ligase of gasdermin D, plays an essential role in promoting GSDMD-induced pyroptotic cell death by mediating K27-linked polyubiquitination of GSDMD at K203 and K204 residues. These findings highlight the potential therapeutic role of GSDMD ubiquitination in inflammatory diseases.
CELL DEATH & DISEASE
(2022)
Article
Medicine, Research & Experimental
Dezhong Zheng, Mingyu Zhang, Tingrong Liu, Tao Zhou, Anna Shen
Summary: The study reveals that OPG induces cardiomyocyte hypertrophy and inhibits autophagy through the FAK/Beclin1 signal pathway. The phosphorylation of FAK plays a key role in this process.
Article
Multidisciplinary Sciences
Tao Yue, Xiaoming Zhan, Duanwu Zhang, Ruchi Jain, Kuan-Wen Wang, Jin Huk Choi, Takuma Misawa, Lijing Su, Jiexia Quan, Sara Hildebrand, Darui Xu, Xiaohong Li, Emre Turer, Lei Sun, Eva Marie Y. Moresco, Bruce Beutler
Summary: ROS in activated T cells triggers an oxidative stress response leading to translation repression, which is countered by SLFN2 binding tRNAs. SLFN2 deficiency results in tRNA fragment accumulation, inhibiting translation and promoting stress-granule formation.
Article
Immunology
Isabel M. Alonso Bellido, Mercedes Posada-Perez, Francisco Hernandez-Rasco, Sandra Vazquez-Reyes, Maria Cabanillas, Antonio J. Herrera, Sara Bachiller, Jesus Soldan-Hidalgo, Ana M. Espinosa-Oliva, Bertrand Joseph, Rocio M. de Pablos, Jose L. Venero, Rocio Ruiz
Summary: The study aims to investigate the role of Caspase-3 in neurogenesis-related microglial functions. Using Caspase-3 conditional knockout mice, it was found that the reduction of Caspase-3 led to decreased microglia and neurogenic neurons in the hippocampus, as well as a lower phagocytic capacity of microglia. Behavioral analysis also showed impaired memory and learning in the absence of Caspase-3. These findings highlight the essential role of Caspase-3 in microglial function and the importance of a specific microglial phenotype in the maintenance of adult hippocampal neurogenesis.
BRAIN BEHAVIOR AND IMMUNITY
(2023)
Article
Biochemistry & Molecular Biology
Hong-Yong Cui, Shi-Jie Wang, Fei Song, Xu Cheng, Gang Nan, Yu Zhao, Mei-Rui Qian, Xi Chen, Jia-Yue Li, Fen-Ling Liu, Yu-Meng Zhu, Ruo-Fei Tian, Bin Wang, Bin Wu, Yang Zhang, Xiu-Xuan Sun, Ting Guo, Xiang-Min Yang, Hai Zhang, Ling Li, Jing Xu, Hui-Jie Bian, Jian-Li Jiang, Zhi-Nan Chen
Summary: Major gaps in understanding the molecular mechanisms of colorectal cancer progression and mucosal repair have hindered therapeutic development for gastrointestinal disorders. TFF3 plays a crucial role in CRC progression and mucosal repair, with upregulated TFF3 predicting worse survival rates in CRC patients. The interaction between TFF3 and CD147 enhances CRC progression by activating STAT3 and PTGS2, leading to increased migration, proliferation, and invasion of cancer cells.
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2021)
Article
Cell Biology
Qing-Rui Wu, Dan-Lin Zheng, Pei-Ming Liu, Hui Yang, Lu-An Li, Su-Juan Kuang, Ying-Yu Lai, Fang Rao, Yu-Mei Xue, Ji-Jin Lin, Shuang-Xin Liu, Chun-Bo Chen, Chun-Yu Deng
Summary: Mitochondrial dysfunction and impaired Ca2+ handling play crucial roles in the development of diabetic cardiomyopathy. Inhibiting Drp1 and Orai1 activity can alleviate cardiomyocyte hypertrophy and dysfunction induced by high glucose.
CELL DEATH & DISEASE
(2021)
Article
Biotechnology & Applied Microbiology
Rio P. Juni, Jordy M. M. Kocken, Ricardo C. Abreu, Lara Ottaviani, Tim Davalan, Burcu Duygu, Ella M. Poels, Aliaksei Vasilevich, Jana C. Hegenbarth, Mahesh Appari, Nicole Bitsch, Serve Olieslagers, Dorien M. Schrijvers, Monika Stoll, Joerg Heineke, Jan de Boer, Leon J. de Windt, Paula A. da Costa
Summary: While impaired myocardial vascularization contributes to a mismatch between myocardial oxygen demand and supply, the mechanistic basis for disrupted tissue growth and angiogenesis in heart failure remains poorly understood. Silencing strategies have identified microRNAs as crucial regulators of angiogenesis, with miR-216a being a pro-angiogenic microRNA enriched in cardiac microvascular endothelial cells. miR-216a null mice display cardiac phenotypes related to impaired myocardial vascularization, autophagy, and inflammation, indicating that microRNA regulation of microvascularization impacts the cardiac response to stress.
Article
Cell Biology
Jun Lin, Qinfeng Li, Tingting Jin, Jiacheng Wang, Yingchao Gong, Qingbo Lv, Meihui Wang, Jiawen Chen, Min Shang, Yanbo Zhao, Guosheng Fu
Summary: IL-1R2 plays a protective role in myocardial I/R injury by protecting cardiomyocytes from apoptosis. Overexpression of IL-1R2 can protect cardiomyocytes from apoptosis by reducing the expression of IL-17RA.
CELL DEATH & DISEASE
(2022)
Article
Cardiac & Cardiovascular Systems
Yu Fang, Shun Wang, Jian Lv, Zhenyi Zhao, Ningning Guo, Gang Wu, Jingjing Tong, Zhihua Wang
Summary: Zinc dyshomeostasis is involved in cardiac hypertrophy, with Slc39a2 playing a key role in regulating zinc uptake and hypertrophic responses. The study also suggests that innate immune signaling pathways are affected by Slc39a2 deficiency, influencing the hypertrophic state of cardiomyocytes.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Hubert Fleury, Myles K. MacEachern, Clara M. Stiefel, Roopesh Anand, Colin Sempeck, Benjamin Nebenfuehr, Kelper Maurer-Alcala, Kerri Ball, Bruce Proctor, Ondrej Belan, Erin Taylor, Raquel Ortega, Benjamin Dodd, Laila Weatherly, Djelika Dansoko, Justin W. Leung, Simon J. Boulton, Nausica Arnoult
Summary: In this study, the APE2 nuclease was identified as an effector of MMEJ repair pathway. It was found that APE2's function in MMEJ depends on its nuclease activity and a previously uncharacterized domain is required for its recruitment to DSBs. This previously unappreciated role of APE2 in MMEJ contributes to the addiction of HRD cells to APE2, which could be exploited in the treatment of cancer.
Article
Immunology
Kei Kato, Kei Haniuda, Saori Fukao, Daisuke Kitamura
Summary: This study demonstrates the essential role of DNA endonuclease DNase1L3 in B cell activation by T cell independent type II (TI-II) antigens. DNase1L3-deficient mice showed a significant reduction in antigen-specific antibody production upon immunization with TI-II antigens, but not with T cell dependent (TD) antigens. Further experiments revealed that B cell-intrinsic DNase1L3 is required for the TI-II response.
INTERNATIONAL IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Carolina Fernandez, Natalia Torrealba, Francisco Altamirano, Valeria Garrido-Moreno, Cesar Vasquez-Trincado, Raul Flores-Vergara, Camila Lopez-Crisosto, Maria Paz Ocaranza, Mario Chiong, Zully Pedrozo, Sergio Lavandero
Summary: Polycystin-1 regulates IGF-1-induced cardiomyocyte hypertrophy through modulating protein tyrosine phosphatase 1B, affecting the activation of IGF-1 receptor, Akt, and ERK1/2. Knockdown of polycystin-1 disrupts the phosphorylation of IGF-1 receptor, Akt, and ERK1/2, leading to impaired cardiomyocyte hypertrophy.
Article
Pharmacology & Pharmacy
Jonathan J. Weldrick, Mohammad Abdul-Ghani, Lynn A. Megeney, Patrick G. Burgon
CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY
(2018)
Review
Cell Biology
Patrick G. Burgon, Lynn A. Megeney
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Marie-Elodie Cattin, Shelley A. Deeke, Sarah A. Dick, Zachary J. A. Verret-Borsos, Gayashan Tennakoon, Rishi Gupta, Esther Mak, Cassandra L. Roeske, Jonathan J. Weldrick, Lynn A. Megeney, Patrick G. Burgon
JOURNAL OF BIOLOGICAL CHEMISTRY
(2018)
Article
Cardiac & Cardiovascular Systems
Charis Putinski, Mohammad Abdul-Ghani, Steve Brunette, Patrick G. Burgon, Lynn A. Megeney
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2018)
Article
Cell Biology
Amit Shrestha, Steve Brunette, William Lloyd Stanford, Lynn Arthur Megeney
Review
Immunology
Sarah A. Dick, Rysa Zaman, Slava Epelman
FRONTIERS IN IMMUNOLOGY
(2019)
Letter
Biochemistry & Molecular Biology
Elena A. Minina, Jens Staal, Vanina E. Alvarez, John A. Berges, Ilana Berman-Frank, Rudi Beyaert, Kay D. Bidle, Frederic Bornancin, Magali Casanova, Juan J. Cazzulo, Chang Jae Choi, Nuria S. Coll, Vishva M. Dixit, Marko Dolinar, Nicolas Fasel, Christiane Funk, Patrick Gallois, Kris Gevaert, Emilio Gutierrez-Beltran, Stephan Hailfinger, Marina Klemencic, Eugene V. Koonin, Daniel Krappmann, Anna Linusson, Mauricio F. M. Machado, Frank Madeo, Lynn A. Megeney, Panagiotis N. Moschou, Jeremy C. Mottram, Thomas Nystrom, Heinz D. Osiewacz, Christopher M. Overall, Kailash C. Pandey, Juergen Ruland, Guy S. Salvesen, Yigong Shi, Andrei Smertenko, Simon Stael, Jerry Stahlberg, Maria Fernanda Suarez, Margot Thome, Hannele Tuominen, Frank Van Breusegem, Renier A. L. van der Hoorn, Assaf Vardi, Boris Zhivotovsky, Eric Lam, Peter V. Bozhkov
Article
Cardiac & Cardiovascular Systems
Jiro Ikeda, Corey A. Scipione, Sharon J. Hyduk, Marwan G. Althagafi, Jawairia Atif, Sarah A. Dick, Maneesha Rajora, Erika Jang, Takuo Emoto, Junichi Murakami, Noriko Ikeda, Hisham M. Ibrahim, Chanele K. Polenz, Xiaotang Gao, Kelly Tai, Jenny Jongstra-Bilen, Ryota Nakashima, Slava Epelman, Clinton S. Robbins, Gang Zheng, Warren L. Lee, Sonya A. MacParland, Myron Cybulsky
Summary: This study revealed that radiation exposure inhibits LDL entry into the aortic intima at baseline and the earliest stages of atherosclerosis in a mouse model of bone marrow transplantation. Single-cell transcriptomic analysis showed a redirection of LDL uptake by endothelial cells to lysosomal degradation and reverse cholesterol transport pathways, reducing lipid accumulation within the intima and impacting lesion initiation and growth.
CIRCULATION RESEARCH
(2021)
Article
Immunology
Sarah A. Dick, Anthony Wong, Homaira Hamidzada, Sara Nejat, Robert Nechanitzky, Shabana Vohra, Brigitte Mueller, Rysa Zaman, Crystal Kantores, Laura Aronoff, Abdul Momen, Duygu Nechanitzky, Wanda Y. Li, Parameswaran Ramachandran, Sarah Q. Crome, Burkhard Becher, Myron Cybulsky, Filio Billia, Shaf Keshavjee, Seema Mital, Clint S. RobbillS, Tak W. Mak, Slava Epelman
Summary: This study reveals the existence of three macrophage subpopulations in mice based on common life cycle properties and core gene signatures. These subpopulations are present in multiple tissues and TLF+ macrophages show the highest transcriptional conservation.
SCIENCE IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Brian D. Larsen, Jan Benada, Philip Yuk Kwong Yung, Ryan A. Bell, George Pappas, Vaclav Urban, Johanna K. Ahlskog, Tia T. Kuo, Pavel Janscak, Lynn A. Megeney, Simon J. Elsaesser, Jiri Bartek, Claus S. Sorensen
Summary: Genotoxic therapies such as radiation can lead to acquired resistance and tumor recurrence. This study reveals that cancer cells maintain viability during radiation by increasing DNA breaks in a reversible manner, preventing premature mitotic progression. The caspase-activated DNase (CAD) is identified as the enzyme responsible for these DNA lesions, which are found near chromatin-modifying CCCTC-binding factor (CTCF) sites. CAD activity is regulated by phosphorylation through DNA damage response kinases, independent of caspase activity. Loss of CAD activity impairs cancer cell fate decisions and renders them more vulnerable to radiation-induced DNA damage.
Article
Clinical Neurology
Osorio Lopes Abath Neto, Livija Medne, Sandra Donkervoort, Maria Elena Rodriguez-Garcia, Veronique Bolduc, Ying Hu, Eleonora Guadagnin, A. Reghan Foley, John F. Brandsema, Allan M. Glanzman, Gihan Tennekoon, Mariarita Santi, Justin H. Berger, Lynn A. Megeney, Hirofumi Komaki, Michio Inoue, Francisco Javier Cotrina-Vinagre, Aurelio Hernandez-Lain, Elena Martin-Hernandez, Linford Williams, Sabine Borell, David Schorling, Kimberly Lin, Konstantinos Kolokotronis, Uta Lichter-Konecki, Janbernd Kirschner, Ichizo Nishino, Brenda Banwell, Francisco Martinez-Azorin, Patrick G. Burgon, Carsten G. Bonnemann
Summary: This study identified seven individuals from six families with biallelic variants in the MLIP gene, shedding light on the genetic landscape of rhabdomyolysis. The findings solidify MLIP's role in maintaining normal and diseased skeletal muscle homeostasis.
Article
Cell Biology
Mohammad Abdul-Ghani, Colin Suen, Baohua Jiang, Yupu Deng, Jonathan J. Weldrick, Charis Putinski, Steve Brunette, Pasan Fernando, Tom T. Lee, Peter Flynn, Frans H. H. Leenen, Patrick G. Burgon, Duncan J. Stewart, Lynn A. Megeney
Review
Biochemistry & Molecular Biology
Ryan A. V. Bell, Lynn A. Megeney
CELL DEATH AND DIFFERENTIATION
(2017)
Review
Cell Biology
Didac Carmona-Gutierrez, Maria Anna Bauer, Andreas Zimmermann, Andres Aguilera, Nicanor Austriaco, Kathryn Ayscough, Rena Balzan, Shoshana Bar-Nun, Antonio Barrientos, Peter Belenky, Marc Blondel, Ralf J. Braun, Michael Breitenbach, William C. Burhans, Sabrina Buttner, Duccio Cavalieri, Michael Chang, Katrina F. Cooper, Manuela Corte-Real, Vitor Costa, Christophe Cullin, Ian Dawes, Jorn Dengjel, Martin B. Dickman, Tobias Eisenberg, Birthe Fahrenkrog, Nicolas Fasel, Kai-Uwe Frohlich, Ali Gargouri, Sergio Giannattasio, Paola Goffrini, Campbell W. Gourlay, Chris M. Grant, Michael T. Greenwood, Nicoletta Guaragnella, Thomas Heger, Juergen Heinisch, Eva Herker, Johannes M. Herrmann, Sebastian Hofer, Antonio Jimenez-Ruiz, Helmut Jungwirth, Katharina Kainz, Dimitrios P. Kontoyiannis, Paula Ludovico, Stephen Manon, Enzo Martegani, Cristina Mazzoni, Lynn A. Megeney, Chris Meisinger, Jens Nielsen, Thomas Nystrom, Heinz D. Osiewacz, Tiago F. Outeiro, Hay-Oak Park, Tobias Pendl, Dina Petranovic, Stephane Picot, Peter Polcic, Ted Powers, Mark Ramsdale, Mark Rinnerthaler, Patrick Rockenfeller, Christoph Ruckenstuhl, Raffael Schaffrath, Maria Segovia, Fedor F. Severin, Amir Sharon, Stephan J. Sigrist, Cornelia Sommer-Ruck, Maria Joao Sousa, Johan M. Thevelein, Karin Thevissen, Vladimir Titorenko, Michel B. Toledano, Mick Tuite, F. -Nora Voegtle, Benedikt Westermann, Joris Winderickx, Silke Wissing, Stefan Woelfl, Zhaojie J. Zhang, Richard Y. Zhao, Bing Zhou, Lorenzo Galluzzi, Guido Kroemer, Frank Madeo