Article
Multidisciplinary Sciences
Eduardo Soto, Jorge E. Galan, Marfa Lara-Tejero
Summary: In this study, an extensive in vivo cross-linking strategy aided by structure modeling was used to investigate the sorting platform complex of type III secretion systems. The assembly process of this bacterial structure was mapped using identified cross-links as signatures for pairwise intersubunit interactions and systematic genetic deletions. Insights generated by this study could be used for the rational development of antivirulence strategies against medically important bacterial pathogens.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Microbiology
Anna Shaliutina-Loginova, Olivera Francetic, Pavel Dolezal
Summary: Bacteria have evolved sophisticated protein secretion nanomachines over billions of years to deliver toxins, enzymes, and proteins. The type II secretion system (T2SS) is used by Gram-negative bacteria to export folded proteins across the outer membrane. Recent findings have shown that components of the T2SS can be found in the mitochondria of certain eukaryotic lineages, suggesting the presence of a mitochondrial T2SS-derived system (miT2SS). This review discusses recent advances in the field and raises questions about the function and evolution of miT2SSs.
Article
Chemistry, Multidisciplinary
Qi Xie, Sea On Lee, Nitya Vissamsetti, Sikao Guo, Margaret E. Johnson, Stephen D. Fried
Summary: Protein-based biomaterials have important roles in tissue engineering and have potential applications as self-healing materials and sustainable polymers. Researchers have discovered that the bacterium Bacillus subtilis can be genetically engineered to secrete silk proteins, which spontaneously assemble into fibers on the cell surface in a process known as secretion-catalyzed assembly (SCA). These secreted silk fibers can form self-healing hydrogels without additional processing, and the fibers retained on the bacterial membrane can be used to create engineered living materials. This study provides a blueprint for the autonomous assembly of protein biomaterials directly from microbial factories.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Microbiology
Kathleen R. Nicholson, Patricia A. Champion
Summary: This article reviews the mechanisms of protein localization and transport in bacteria, highlighting the conserved themes of regulation and environmental sensing in bacterial secretion systems.
Article
Microbiology
Timothy A. Klein, Dirk W. Grebenc, Prakhar Y. Shah, Owen D. McArthur, Brandon H. Dickson, Michael G. Surette, Youngchang Kim, John C. Whitney
Summary: Type VIIb secretion systems (T7SSb) are utilized by various Gram-positive bacteria to facilitate interbacterial killing and pathogenesis through the secretion of toxins. This study discovered two new protein families, Lap1 and Lap2, that interact with these toxins and are essential for their secretion, providing new insights into T7SSb-dependent toxin release from Gram-positive bacteria.
Article
Biochemistry & Molecular Biology
Nidhi Singh, Thales Kronenberger, Andrea Eipper, Felix Weichel, Mirita Franz-Wachtel, Boris Macek, Samuel Wagner
Summary: The study found that the salt bridges in the assembly of virulence-associated T3SS are not critical, but facilitate the incorporation of the next subunit. The conserved charged residues are crucial for type III-dependent secretion and play a key role in the function of T3SS.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Immunology
Magali G. Bialer, Mariana C. Ferrero, M. Victoria Delpino, Veronica Ruiz-Ranwez, Diana M. Posadas, Pablo C. Baldi, Angeles Zorreguieta
Summary: Adhesion to host cells is essential for successful infection of bacterial pathogens. Brucella suis 1330 harbors three type Va autotransporters (BmaA, BmaB, and BmaC), all playing a role in adhesion to different cell types. These autotransporters with significant sequence similarities contribute to the adhesive properties of B. suis 1330 and display interesting variations among Brucella species.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Review
Dentistry, Oral Surgery & Medicine
P. D. Veith, M. D. Glew, D. G. Gorasia, E. Cascales, E. C. Reynolds
Summary: Bacteria in severe periodontitis utilize the T9SS system to release virulence factors that dysregulate the host immune response, leading to chronic inflammatory destruction of tooth supporting tissues. The molecular structure and secretion mechanism of T9SS are crucial for understanding this process.
JOURNAL OF DENTAL RESEARCH
(2022)
Review
Biochemistry & Molecular Biology
Amit Meir, Kevin Mace, Yogesh Vegunta, Sunanda M. Williams, Gabriel Waksman
Summary: This review provides an update on recent advances in substrate recruitment and delivery by recruitment platforms associated with Type III, IV, and VI secretion systems in gram-negative bacteria.
TRENDS IN MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Lenka Horvathova, Vojtech Zarsky, Tomas Panek, Romain Derelle, Jan Pyrih, Alzbeta Motyckova, Veronika Klapst'ova, Martina Vinopalova, Lenka Markova, Lubos Voleman, Vladimir Klimes, Marketa Petru, Zuzana Vaitova, Ivan Cepicka, Klara Hryzakova, Karel Harant, Michael W. Gray, Mohamed Chami, Ingrid Guilvout, Olivera Francetic, B. Franz Lang, Cestmir Vlcek, Anastasios D. Tsaousis, Marek Elias, Pavel Dolezal
Summary: The study reveals that certain primitive eukaryotes possess T2SS components similar to those found in Gram-negative bacteria, with some of them located in mitochondria. They are part of a broader functional pathway involving other proteins in eukaryotes.
NATURE COMMUNICATIONS
(2021)
Article
Biology
Owain J. Bryant, Paraminder Dhillon, Colin Hughes, Gillian M. Fraser
Summary: In addition to the gate recognition motif (GRM), a hydrophobic N-terminal signal is identified on flagellar rod and hook subunits, which are separately and sequentially recognized by the flagellar export machinery. The hydrophobic N-terminal signal may trigger the opening of the flagellar export gate.
Article
Biology
Jianfeng Zhang, Jiahao Guan, Meng Wang, Gang Li, Marko Djordjevic, Cui Tai, Hui Wang, Zixin Deng, Zhaoyan Chen, Hong-Yu Ou
Summary: The Type VI Secretion System (T6SS) plays a significant role in microbial activities by injecting effectors into adjacent cells or environments. Its importance in pathogenesis and microbial competition has led to an increasing focus on T6SS-related research. To aid in T6SS research, SecReT6 version 3 provides comprehensive information on T6SS and its interactions with related proteins, along with tools for detection, analysis, and estimation of T6SS-dependent killing risk. This resource has been instrumental in identifying a novel T6SS regulator and T6SS-dependent killing capacity in Acinetobacter baumannii clinical isolates. Additionally, a database containing 17,212 T6SSs and numerous T6SS-related proteins from 26,573 bacterial complete genomes has been developed and made freely available.
SCIENCE CHINA-LIFE SCIENCES
(2023)
Review
Chemistry, Multidisciplinary
Emily Lundstedt, Daniel Kahne, Natividad Ruiz
Summary: The asymmetric lipid bilayer of Gram-negative bacteria's outer membrane is crucial for antibiotic resistance, assembled by specific protein machines such as the Lpt system and Mla pathway.
Article
Biochemistry & Molecular Biology
Maksym Bobrovskyy, So Young Oh, Dominique Missiakas
Summary: In this study, researchers discovered that EssC recruits EsaA, EssA, and EssB to form a complex called the ESS translocon in the type 7b secretion system of Staphylococcus aureus. The assembly of the ESS translocon does not require secreted substrates. FHA1 and DUF are essential for the stability of EssC, while FHA2 is necessary for its association with EssB.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Kinya Nomura, Felipe Andreazza, Jie Cheng, Ke Dong, Pei Zhou, Sheng Yang He
Summary: The AvrE family of bacterial effector proteins functions as membrane channels in plant cells, facilitating pathogen growth and disrupting host cell viability. The elucidation of the biochemical function of AvrE family effector proteins is of great significance in understanding bacterial pathogenesis.