Article
Oncology
Qizhi Qin, Mario Gomez-Salazar, Robert J. Tower, Leslie Chang, Carol D. Morris, Edward F. McCarthy, Kang Ting, Xinli Zhang, Aaron W. James
Summary: The study reveals that NELL1, a secreted glycoprotein, plays a crucial role in sarcoma progression and prognosis by modulating cell invasion potential. Deletion of NELL1 significantly reduces metastasis and disease progression in osteosarcoma, both in human and mouse models. Further analysis shows that NELL1 loss alters the expression of matricellular proteins associated with cell signaling.
Article
Biochemistry & Molecular Biology
Dapei Li, Lifen Xie, Zigang Qiao, Sanyue Mai, Jingfei Zhu, Fan Zhang, Shengchuan Chen, Liang Li, Fangrong Shen, Yanghua Qin, Haiping Yao, Sudan He, Feng Ma
Summary: The study reveals that IFI16 and the p53 pathway activator nutlin-3 synergistically induce apoptosis in U2OS and A549 cells, and phosphorylation of p53 at serine 392 by protein kinase R plays a critical role in IFI16-p53-dependent apoptosis. Overexpression of STING suppresses p53 serine 392 phosphorylation, p53 transcriptional activity, and p53-dependent mitochondrial depolarization and apoptosis, indicating a protumorigenic role for STING in certain cancer types by degrading upstream IFI16.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Michael J. O'Connor, Tanay Thakar, Claudia M. Nicolae, George-Lucian Moldovan
Summary: PARP14 is identified as a regulator of cyclin D1 expression, with its depletion leading to decreased cyclin D1 protein levels and G1 cell-cycle arrest. This regulation is achieved through controlling cyclin D1 mRNA levels and is dependent on an intact p53-p21 pathway. The findings highlight a new role for PARP14 in promoting cell-cycle progression through cyclin D1 and the p53 pathway.
Article
Cell Biology
Mi-Jeong Kim, Yoon Min, Soo-Kyung Jeong, Juhee Son, Ji Young Kim, Ji Su Lee, Duk-Hwan Kim, Joo Sang Lee, Eunyoung Chun, Ki-Young Lee
Summary: The study reveals that USP15 is significantly downregulated in lung cancer patients, and its knockout leads to increased migration, invasion, and autophagy induction in lung cancer cells. USP15 interacts with BECN1 and deubiquitinates it, thereby attenuating autophagy induction. Additionally, the expression of USP15 is associated with the upregulation of certain oncogenes and downregulation of tumor suppressor genes in lung cancer patients.
CELL DEATH & DISEASE
(2022)
Article
Chemistry, Multidisciplinary
Debao Fang, Hao Hu, Kailiang Zhao, Aman Xu, Changjun Yu, Yong Zhu, Ning Yu, Bo Yao, Suyun Tang, Xianning Wu, Yide Mei
Summary: MLF2 is a new negative regulator of p53 that interacts with both p53 and USP7. It inhibits USP7-mediated deubiquitination of p53, leading to p53 destabilization and promoting colorectal cancer. Clinically, MLF2 is elevated in colorectal cancer and its high expression is associated with poor prognosis.
Article
Cell Biology
Lei Yao, Jun Li, Bo Jiang, Zeyu Zhang, Xinying Li, Xiwu Ouyang, Yao Xiao, Guodong Liu, Zhiming Wang, Gewen Zhang
Summary: RNF2 is a RING domain-containing E3 ubiquitin ligase that mediates the mono-ubiquitination of histone H2A to repress gene transcription. Its expression patterns and molecular function in hepatocellular carcinoma (HCC) were investigated in this study. The results showed that RNF2 was highly expressed in HCC and was associated with malignant characteristics and poor prognosis. Additionally, RNF2 was found to promote HCC metastasis through enhancing epithelial-mesenchymal transition (EMT).
CELL DEATH & DISEASE
(2023)
Article
Plant Sciences
Jing Guo, Yaqiong Wu, Tongli Wang, Yue Xin, Guibin Wang, Qi Zhou, Li-An Xu
Summary: Flavonoids in ginkgo are important secondary metabolites with extensive pharmacological functions. However, the biosynthesis of ginkgo flavonols is not well understood. In this study, the GbFLSa gene was cloned and found to potentially negatively regulate the biosynthesis of proanthocyanins. This study is significant for elucidating the role of GbFLSa in ginkgo metabolism and the molecular mechanism of flavonoid biosynthesis.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Yajun Li, Kaisa Cui, Qiang Zhang, Xu Li, Xingrong Lin, Yi Tang, Edward Prochownik, Youjun Li
Summary: This study identified the amplified and over-expressed FBXL6 in colorectal cancer patients, which is highly correlated with poor prognosis, and revealed its mechanism in suppressing p53 signaling by targeting phospho-p53 for degradation. The feed-forward loop of FBXL6-p53, where p53 transcriptionally suppresses FBXL6 expression, might serve as a potential therapeutic target for CRC treatments.
CELL DEATH AND DIFFERENTIATION
(2021)
Article
Multidisciplinary Sciences
Dapeng Ma, Min Yang, Qiushi Wang, Caiyu Sun, Hongbiao Shi, Weiqiang Jing, Yuxuan Bi, Xuecheng Shen, Xiaomin Ma, Zhenzhi Qin, Yueke Lin, Lihui Zhu, Yunxue Zhao, Yeping Cheng, Lihui Han
Summary: PRMT5 catalyzes arginine symmetrical dimethylation of cGAS, attenuating the antiviral immune response by blocking cGAS' DNA binding ability. Inhibitors of PRMT5 protect mice from HSV-1 infection and prolong their survival time, indicating the potential of modulating PRMT5 as an antiviral strategy.
Article
Biochemistry & Molecular Biology
Wei Wang, Xiang-Dong Bai, Kun Chen, Xiao-Yue Zhang, Chen-Rui Gu, Jing Jiang, Chuan-Ping Yang, Gui-Feng Liu
Summary: This study found that the interference expression of the PsnWRKY70 gene can enhance the tolerance of poplar to saline-alkali stress and has a positive effect on the growth and alkali damage of this tree species. Transcriptome analysis showed that the interference expression of the PsnWRKY70 gene up-regulated multiple genes related to cell wall organization or biogenesis pathway, which play an important role in NaHCO3 stress.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Naga Padma Lakshmi Ch, Ananthi Sivagnanam, Sebastian Raja, Sundarasamy Mahalingam
Summary: RASSF10 inhibits Cdk1/cyclin-B complex formation to induce mitotic arrest and inhibit cell proliferation, while promoting nuclear accumulation of GADD45a. Nucleophosmin (NPM) is a novel functional target of RASSF10, critical for RASSF10-mediated G2/M phase arrest. Knockdown of NPM or GADD45a impairs RASSF10-mediated cell cycle regulation.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Cell Biology
Wendy A. Herbst, Weixian Deng, James A. Wohlschlegel, Jennifer M. Achiro, Kelsey C. Martin
Summary: By using nucleus-specific proteomic mapping in silenced and stimulated neurons, researchers revealed an understudied mechanism of nuclear proteome regulation: activity-dependent proteasome-mediated degradation. They found that the tumor suppressor protein PDCD4 undergoes rapid stimulus-induced degradation in the nucleus of neurons. This degradation of PDCD4 is crucial for normal activity-dependent transcription, impacting genes involved in synapse formation, remodeling, and transmission.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Microbiology
Meiling Jing, Ting Zheng, Tao Gong, Jiangchuan Yan, Jiamin Chen, Yongwang Lin, Boyu Tang, Qizhao Ma, Xuedong Zhou, Yuqing Li
Summary: This study identified a transcription factor called AhrC, which negatively regulates arginine biosynthesis and biofilm formation in S. mutans. The deletion of ahrC in strains resulted in slow growth and increased intracellular arginine content, while overexpression of ahrC led to reduced arginine content, decreased biofilm and altered biofilm structures. The analysis also revealed specific DNA patterns bound by AhrC, and it acted as a negative regulator. These findings contribute to our understanding of the regulation of arginine biosynthesis and biofilm formation in S. mutans.
MICROBIOLOGY SPECTRUM
(2022)
Article
Biochemistry & Molecular Biology
Kimiyoshi Yano, Ryou-U Takahashi, Bunsyo Shiotani, Junko Abe, Tomoki Shidooka, Yuki Sudo, Yusuke Yamamoto, Shisei Kan, Hiroki Sakagami, Hidetoshi Tahara
Summary: PRPF19 is identified as a critical regulator of cellular senescence in normal human fibroblasts by modulating MDM4 splicing and p53 activity, leading to induction of p53-dependent cellular senescence.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Dentistry, Oral Surgery & Medicine
J. Chen, A. Zhang, Z. Xiang, M. Lu, P. Huang, T. Gong, Y. Pan, Y. Lin, X. Zhou, Y. Li
Summary: EpsR, a MarR family transcription factor, negatively regulates gtfB expression and exopolysaccharide production in S. mutans. Deletion of epsR led to altered colony morphology, increased water-insoluble EPS production, and upregulated GtfB protein content and activity, indicating the importance of EpsR in regulating cariogenic biofilm formation.
JOURNAL OF DENTAL RESEARCH
(2021)
Article
Developmental Biology
Feng Cheng, Liyun Miao, Qing Wu, Xia Gong, Jingwei Xiong, Jian Zhang
Article
Developmental Biology
Liyun Miao, Yue Yuan, Feng Cheng, Junshun Fang, Fang Zhou, Weirui Ma, Yan Jiang, Xiahe Huang, Yingchun Wang, Lingjuan Shan, Dahua Chen, Jian Zhang
Article
Biochemistry & Molecular Biology
Suhong Xu, Feng Cheng, Juan Liang, Wei Wu, Jian Zhang
Article
Oncology
Yang Yu, Yanling Zhao, Jongmin Choi, Zhongcheng Shi, Linjie Guo, John Elizarraras, Andy Gu, Feng Cheng, Yanxin Pei, Dai Lu, Muller Fabbri, Saurabh Agarwal, Chunchao Zhang, Sung Yun Jung, Jennifer H. Foster, Jianhua Yang
Summary: The study demonstrates that the ERK inhibitor ulixertinib has therapeutic potential for neuroblastoma (NB) by inhibiting cell proliferation, tumor growth, and promoting cell sensitivity to chemotherapeutic drugs. The findings provide preclinical evidence for ulixertinib as a novel therapeutic approach for NB.
Article
Multidisciplinary Sciences
Lawrence Huang, Feng Cheng, Xuetao Zhang, Jacek Zielonka, Matthew A. Nystoriak, Weiwei Xiang, Kunal Raygor, Shaoxun Wang, Aditya Lakshmanan, Weiya Jiang, Sai Yuan, Kevin S. Hou, Jiayi Zhang, Xitao Wang, Arsalan U. Syed, Matea Juric, Takamune Takahashi, Manuel F. Navedo, Rong A. Wang
Summary: The mechanisms underlying arteriovenous malformations (AVMs) are still poorly understood. This study used mice with endothelial cell (EC) expression of constitutively active Notch4 and found that decreased arteriolar tone is associated with AVM initiation. The reduced vascular tone is a primary effect of Notch4*EC, and the nitric oxide synthase (NOS) inhibitor L-NNA corrected the vascular tone defects. The involvement of eNOS in AVM formation is suggested by its up-regulation of hydrogen peroxide and its role in reducing vascular tone.