Article
Biochemistry & Molecular Biology
Ana Clara Lagazzi Cressoni, Leticia B. C. Penariol, Cristiana Carolina Padovan, Maristela D. Orellana, Julio Cesar Rosa-e-Silva, Omero Benedicto Poli-Neto, Rui Alberto Ferriani, Claudia Cristina Paro de Paz, Juliana Meola
Summary: Menstrual blood mesenchymal stem cells (MenSCs) are a noninvasive source with potential clinical applications in regenerative medicine. In endometriotic MenSCs, miRNAs are involved in regulating important cellular processes. However, the miRNA biogenesis pathway in endometriotic MenSCs has not been studied.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Manish Pandey, Arthur Luhur, Nicholas S. S. Sokol, Geetanjali Chawla
Summary: This study investigated the impact of position, order, stem-base, and terminal loop sequences on the differential processing of miRNAs in clustered microRNAs. The results highlighted the importance of structural attributes in regulating the expression of polycistronic miRNAs and provided specific guidelines for achieving differential expression within a cluster.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Shaohua Li, Thi Nhu-Y Le, Trung Duc Nguyen, Tam Anh Trinh, Tuan Anh Nguyen
Summary: Research shows that bulges in pri-miRNAs can modulate the cleavage efficiency and accuracy of miRNA processing by influencing the activity and orientation of the Microprocessor complex, suggesting their potential roles in regulating miRNA biogenesis.
Article
Biochemistry & Molecular Biology
Anton Zhiyanov, Stepan Nersisyan, Alexander Tonevitsky
Summary: MiRNA isoforms (isomiRs) are single-stranded small RNAs produced from the same pri-miRNA hairpin through cleavage by Drosha and Dicer enzymes. Analysis of miRNA processing positions across 31 different cancers revealed that the positions are not tissue-specific and a model based on surrounding nucleotides can distinguish between homogeneous and heterogeneous cleavage. Additionally, some miRNAs show imprecise Dicer cleavage at the 5' end, while almost all cleavage sites at the 3' end are heterogeneous.
Article
Biochemistry & Molecular Biology
Abel Fothi, Orsolya Biro, Zsuzsa Erdei, Agota Apati, Tamas I. Orban
Summary: The study reveals tissue-specific and position-dependent differences in expression levels of C19MC miRNA cluster in embryonic stem cells and placenta, possibly due to differences in posttranscriptional processing. In placenta, there are enhancer regions associated with the Microprocessor complex, leading to more efficient processing of pri-miRNA.
Article
Oncology
Hiresh Ayoubian, Joana Heinzelmann, Sebastian Hoelters, Oybek Khalmurzaev, Alexey Pryalukhin, Philine Loertzer, Julia Heinzelbecker, Stefan Lohse, Carol Geppert, Hagen Loertzer, Heiko Wunderlich, Rainer M. Bohle, Michael Stoeckle, Vsevolod Borisovich Matveev, Arndt Hartmann, Kerstin Junker
Summary: Specific miRNAs were identified as potential diagnostic and prognostic markers in different histological subtypes of penile squamous cell carcinoma (PSCC) and were associated with HPV infection. Microarray analyses revealed significant differences in expression patterns between distinct histological subtypes and metastatic PSCC.
Article
Biochemistry & Molecular Biology
Jan Roska, Joao Lobo, Danica Ivovic, Lenka Wachsmannova, Thomas Mueller, Rui Henrique, Carmen Jeronimo, Miroslav Chovanec, Dana Jurkovicova
Summary: Testicular germ cell tumours (TGCTs) are the most common solid malignancy among young men, and their incidence is still increasing. We identified specific miRNA expression patterns that discriminate chemoresistant phenotypes in TGCTs and proposed miR-218-5p, miR-31-5p, miR-375-5p, miR-517-3p, miR-20b-5p and miR-378a-3p as potential biomarkers for predicting chemoresistance and aggressive phenotypes in non-seminomatous TGCTs. These miRNAs were found to be upregulated in CDDP-resistant TGCT cell lines, tumor samples from metastatic TGCT patients, and plasma samples from TGCT patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biology
I. Ormenezi, A. Ribeiro-Silva, J. C. Rosa-e-Silva, J. Meola, F. J. Candido-dos-Reis, O. B. Poli-Neto
Summary: This study evaluated the expression of Dicer, Drosha, and Exportin-5 in the eutopic and ectopic endometrium of women with adenomyosis. The results showed lower Drosha expression in the eutopic endometrium of women with adenomyosis compared to women without the disease, and a correlation between Drosha expression in the ectopic and paired eutopic endometrium. No significant difference was observed in Dicer or Exportin expression.
BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH
(2022)
Review
Biochemistry & Molecular Biology
Xuanshuo Wei, Jin Tang, Chuwen Lin, Xuan Jiang
Summary: Besides its role in miRNA biogenesis, Drosha also has various functions including promoting DNA damage repair, transcriptional regulation, mRNA stability control, etc. This review provides an overview of the diverse functions of Drosha in novel biological processes.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Multidisciplinary Sciences
Lin Wang, Xingxing Yan, Yanjun Li, Zhiye Wang, Shweta Chhajed, Baoshuan Shang, Zhen Wang, Suk Won Choi, Hongwei Zhao, Sixue Chen, Xiuren Zhang
Summary: Phosphorylation can regulate miRNA metabolism by controlling the accumulation balance of SE and quickly clearing excessive SE.
Article
Virology
Sophie C. Anker, Marie G. Szczeponik, Jan Dessila, Katia Dittus, Christine E. Engeland, Dirk Jaeger, Guy Ungerechts, Mathias F. Leber
Summary: Virotherapy is being investigated as a promising form of cancer immunotherapy, but its anti-tumor potency needs improvement. One limitation is the activation of antiviral defense pathways by tumor cells, which hinders the replication efficiency of oncolytic viruses. This study developed a universal expression cassette for artificial microRNAs that can suppress genes of interest, potentially improving the effectiveness of virotherapy.
Article
Biochemistry & Molecular Biology
Tomas Kupec, Andreas Bleilevens, Birgit Klein, Thomas Hansen, Laila Najjari, Julia Wittenborn, Elmar Stickeler, Jochen Maurer
Summary: This study investigated the expression of miRNAs in urine and serum of ovarian cancer patients using a human miRNA microarray chip. The findings suggest that miRNA profiling in urine may be more suitable than serum for identifying biomarkers in ovarian cancer.
Review
Plant Sciences
Jayanti Jodder
Summary: miRNAs in plants play a crucial role in controlling growth, development, and stress tolerance by modulating the expression of target genes. Modulating the expression of specific stress/development-related genes can be achieved by targeting the corresponding miRNAs. Understanding the molecular mechanism of miRNA biogenesis, particularly the processing of pri-MIRNAs, is essential for effectively modulating miRNA expression levels and ultimately improving plant yield and stress tolerance.
PLANT CELL REPORTS
(2021)
Article
Fisheries
Ye Ge, Qiucheng Yao, Yingying Wang, Qing Wang, Weiwei Zeng
Summary: The study utilized miRNA library construction and microarray sequencing to screen out miRNAs with significant differences between the GCRV infection group and the control group. The results showed the importance of related miRNAs and mRNA target genes in immune effect processes, regulation of immune effect processes, cell death, regulation of leucocyte-mediated cytotoxicity, potentially involving deregulation of the PI3K/AKT, Toll-like, and RIG-I signaling pathways.
AQUACULTURE RESEARCH
(2021)
Article
Oncology
George Lambrou, Myrto Poulou, Krinio Giannikou, Marios Themistocleous, Apostolos Zaravinos, Maria Braoudaki
Summary: Epigenetic mechanisms, particularly miRNA expression and DNA methylation, play a significant role in childhood central nervous system tumors. The study identified frequent methylation of genes like CASP8 and RASSF1 in tumor samples, which correlated with tumor staging and type. Simultaneous methylation of multiple genes' promoters showed significant results, indicating the importance of epigenetic regulation in tumor progression and pathophysiology.
Article
Oncology
Yuta Imaizumi, Saishu Yoshida, Yumi Kanegae, Ken Eto, Kiyotsugu Yoshida
Summary: In colorectal cancer, the expression of DYRK2 is decreased, affecting clinical prognosis. Overexpression of DYRK2 through adenovirus-mediated gene transfer can inhibit cell proliferation and induce apoptosis, providing a potential therapy for colorectal cancer.
Review
Gastroenterology & Hepatology
Kohji Yamada, Kiyotsugu Yoshida
Summary: Protein kinase C delta (PKC delta) is a member of the PKC family and plays important roles in various biological and cancerous processes. Its function is regulated by factors such as phosphorylation and subcellular localization. Recent studies have found its unique extracellular localization and growth factor-like function in liver cancer cells, contributing to our understanding of its involvement in liver cancer progression.
WORLD JOURNAL OF GASTROENTEROLOGY
(2022)
Article
Oncology
Kohji Yamada, Ryusuke Kizawa, Ayano Yoshida, Rei Koizumi, Saya Motohashi, Yuya Shimoyama, Yoshito Hannya, Saishu Yoshida, Tsunekazu Oikawa, Masayuki Shimoda, Kiyotsugu Yoshida
Summary: PKC delta is secreted from liver cancer cells and binds to the epidermal growth factor receptor (EGFR), activating the EGFR signaling pathway and promoting cell proliferation and tumor growth in liver cancer.
Article
Cell Biology
Akira Kawamura, Saishu Yoshida, Katsuhiko Aoki, Yuya Shimoyama, Kohji Yamada, Kiyotsugu Yoshida
Summary: This study reveals that dual-specificity tyrosine-regulated kinase 2 (DYRK2) is a novel regulator of neddylation, promoting neddylation through forming a complex with NEDD8-activating enzyme E1 component NAE1, and thus maintaining genome stability.
JOURNAL OF CELL SCIENCE
(2022)
Article
Multidisciplinary Sciences
Kohji Yamada, Saya Motohashi, Tsunekazu Oikawa, Naoko Tago, Rei Koizumi, Masaya Ono, Toshiaki Tachibana, Ayano Yoshida, Saishu Yoshida, Masayuki Shimoda, Masahiro Oka, Yoshihiro Yoneda, Kiyotsugu Yoshida
Summary: The study reveals the role of E-Syt1 in mediating cytosolic protein secretion at the ER-PM contact sites in liver cancer cells. SEC22B vesicles tethered to the PM via Q-SNAREs for their secretion, while PKC delta secretion and tumorigenicity were regulated by the interaction with E-Syt1.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Oncology
Yuya Shimoyama, Kohji Yamada, Saishu Yoshida, Akira Kawamura, Yoshito Hannya, Yuta Imaizumi, Tomotaka Kumamoto, Yasuhiro Takeda, Masayuki Shimoda, Ken Eto, Kiyotsugu Yoshida
Summary: This study investigates the role of PKC delta in colorectal cancer (CRC) and finds that PKC delta acts as a negative regulator of cellular senescence in p53 wild-type CRC. The activation of p21 via a p53-independent pathway by PKC delta is shown to be essential for its activity. The study also proposes a novel therapeutic strategy for CRC targeting wt-p53.
Article
Biochemistry & Molecular Biology
Kohji Yamada, Kiyotsugu Yoshida
Summary: Unconventional protein secretion (UPS) is a crucial mechanism controlling the localization of cytosolic proteins lacking signal peptides and is implicated in inflammation, neurodegenerative diseases, and cancer. This article provides an overview of UPS mechanisms and categorizes cancer-specific UPS as CUPS due to their distinct features in cancer cells.
DNA AND CELL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Kohji Yamada, Yoshito Hannya, Tsunekazu Oikawa, Ayano Yoshida, Kuniko Katagiri, Saishu Yoshida, Rei Koizumi, Naoko Tago, Yuya Shimoyama, Akira Kawamura, Yuta Mochimaru, Ken Eto, Kiyotsugu Yoshida
Summary: This study demonstrates that E-Syt1 plays a critical role in the tumorigenic potential of liver cancer cells. E-Syt1 depletion inhibits cell proliferation and is associated with a poorer prognosis in hepatocellular carcinoma. Furthermore, E-Syt1 is required for the unconventional secretion of PKCd and the activation of IGF1R and Erk1/2 signaling pathways, which are implicated in liver cancer tumorigenesis.
Review
Biochemistry & Molecular Biology
Saishu Yoshida, Kiyotsugu Yoshida
Summary: The DYRK family, from invertebrates to mammals, plays a crucial role in regulating cell processes such as proliferation, apoptosis, survival, and differentiation. However, limited evidence exists regarding their in vivo roles in mammalian development. This review provides an overview of in vivo studies on Dyrks, shedding light on their roles in mammalian tissue development and congenital diseases.
Article
Oncology
Yasuhiro Takano, Satomi Yogosawa, Yuta Imaizumi, Hiroshi Kamioka, Yumi Kanegae, Ken Eto, Kiyotsugu Yoshida
Summary: DYRK2 expression is associated with chemosensitivity to 5-fluorouracil and oxaliplatin in p53 wild-type colorectal cancer, suggesting its importance as a new marker in therapeutic strategies.
Article
Gastroenterology & Hepatology
Hiroshi Kamioka, Satomi Yogosawa, Tsunekazu Oikawa, Daisuke Aizawa, Kaoru Ueda, Chisato Saeki, Koichiro Haruki, Masayuki Shimoda, Toru Ikegami, Yuji Nishikawa, Masayuki Saruta, Kiyotsugu Yoshida
Summary: DYRK2 has been found to play an anti-tumor role in hepatocellular carcinoma (HCC) by promoting the degradation of Myc and Hras. This study provides new insights into the potential therapeutic approach of using DYRK2 gene transfer for HCC treatment.
Meeting Abstract
Oncology
Yoshimi Imawari, Rei Mimoto, Kiyotsugu Yoshida
Review
Biochemistry & Molecular Biology
Yuta Mochimaru, Kiyotsugu Yoshida
Summary: DYRK2 plays diverse roles in cancer, with controversy regarding its functional conservation. Although studies have been conducted in different types of cancer, the current methods used are not sufficient to fully comprehend the role of DYRK2 in cancer.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2023)
Meeting Abstract
Oncology
Kohji Yamada, Tsunekazu Oikawa, Saya Motohashi, Ayano Yoshida, Rei Koizumi, Saishu Yoshida, Kiyotsugu Yoshida
Meeting Abstract
Oncology
Saya Motohashi, Kohji Yamada, Ayano Yoshida, Rei Koizumi, Kiyotsugu Yoshida