Article
Oncology
Jennifer M. SanMiguel, Elizabeth Eudy, Matthew A. Loberg, Kira A. Young, Jayna J. Mistry, Kristina D. Mujica, Logan S. Schwartz, Timothy M. Stearns, Grant A. Challen, Jennifer J. Trowbridge
Summary: Clonal hematopoiesis resulting from enhanced fitness of mutant hematopoietic stem cells can lead to both favorable and unfavorable health outcomes. This study found that aging-induced TNF alpha signaling promotes the selective advantage of mutant HSCs and stimulates the production of mutant B lymphoid cells. The loss of TNFR1 abolishes the advantage of mutant HSCs, while the loss of TNFR2 results in overproduction of mutant myeloid cells without affecting HSC fitness.
Article
Cell & Tissue Engineering
Ece Somuncular, Julia Hauenstein, Prajakta Khalkar, Anne-Sofie Johansson, Ozge Dumral, Nicolai S. Frengen, Charlotte Gustafsson, Giuseppe Mocci, Tsu-Yi Su, Hugo Brouwer, Christine L. Trautmann, Michael Vanlandewijck, Stuart H. Orkin, Robert Mansson, Sidinh Luc
Summary: This study reveals the heterogeneity of hematopoietic stem cells (HSCs) and provides insights into the functional differences and regulatory mechanisms of HSCs through transcriptional and epigenetic mechanisms.
Article
Biotechnology & Applied Microbiology
Azmeer Sharipol, Maggie L. Lesch, Celia A. Soto, Benjamin J. Frisch
Summary: This study developed a modular murine bone marrow microenvironment-on-chip that can maintain functional hematopoietic stem cells in vitro. The chip model has a better representation of the human bone marrow, which contributes to a deeper understanding of bone marrow pathophysiology.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Lisa Bast, Michele C. Buck, Judith S. Hecker, Robert A. J. Oostendorp, Katharina S. Goetze, Carsten Marr
Summary: Traditionally, hematopoietic stem cell differentiation was believed to occur in a specific hierarchical manner, but recent research has shown alternative pathways. Through experimental data, mathematical models, and samples from healthy human donors, it was found that the classical hematopoiesis model is preferred in most cases, with exceptions where differentiation of multipotent lymphoid progenitors into granulocyte-monocyte progenitors is plausible.
Review
Pharmacology & Pharmacy
Cornelia Lee-Thedieck, Peter Schertl, Gerd Klein
Summary: This review provides a comprehensive overview of the extracellular matrix (ECM) in hematopoietic stem cell (HSC) niches and highlights its importance in regulating cellular function and niche structure. The role of different classes of ECM molecules and their interactions with cells are discussed, along with the significance of matrix remodeling and biophysics in HSC niche function. The review also examines the application of current knowledge of ECM in artificial HSC niches for HSC expansion, targeted differentiation, and drug testing.
ADVANCED DRUG DELIVERY REVIEWS
(2022)
Article
Microbiology
Shiela McCollam, James S. Lewis, Joseph Bubalo, Amber Diaz
Summary: This study aimed to evaluate the efficacy and safety of 300-mg once-monthly intravenous (IV) pentamidine prophylaxis in 702 adult allogeneic hematopoietic stem cell transplant (HSCT) patients. The results showed that IV pentamidine administration effectively prevented Pneumocystis jirovecii pneumonia (PJP), but breakthrough Nocardia and Toxoplasma infections were observed in a certain percentage of patients.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Biochemical Research Methods
Hyebeen Hong, Yoontae Lee
Summary: This article describes a method using transgenic and knockout mice to study the role of specific genes in hematopoiesis in vivo. The protocols for retrovirus preparation, transduction of fetal liver cells, and generation of chimeric mice are detailed, and applicable for studying the functionality of genes of interest in immune cells.
Review
Hematology
Maria Jassinskaja, Monika Gonka, David G. Kent
Summary: One of the major challenges in stem cell research is determining their function through retrospective assays. This is particularly difficult for hematopoietic stem cell (HSC) research, as the current functional assay requires long-term transplantation that destroys the initial cell state before confirming its HSC identity. With the development of single-cell molecular assays, researchers are faced with the paradox of determining the molecular state of a functional HSC without simultaneously assessing its functionality and molecular properties.
Article
Biochemistry & Molecular Biology
Asaka Miura, Takashi Shimbo, Tomomi Kitayama, Yuya Ouchi, Sho Yamazaki, Mami Nishida, Eiichi Takaki, Ryoma Yamamoto, Edward Wijaya, Katsuto Tamai
Summary: The study systematically evaluated the characteristics of PDGFR alpha Lin+ HCs in adult mice, showing their presence in bone marrow and peripheral blood and similar transcriptomic signatures to PDGFR alpha Lin- HCs at the single-cell level. Although pathophysiological activities were not evaluated, the data clearly demonstrate a significant role of PDGFR alpha Lin + HCs in physiological hematopoiesis in adult mice.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Cell Biology
Anindita Roy, Guanlin Wang, Deena Iskander, Sorcha O'Byrne, Natalina Elliott, Jennifer O'Sullivan, Gemma Buck, Elisabeth F. Heuston, Wei Xiong Wen, Alba Rodriguez Meira, Peng Hua, Anastasios Karadimitris, Adam J. Mead, David M. Bodine, Irene Roberts, Bethan Psaila, Supat Thongjuea
Summary: Human hematopoiesis is a dynamic process that starts in utero 18-21 days post-conception, and understanding the site- and stage-specific variations is crucial for unraveling the origin of hematological disorders. The comparison of 57,489 HSPCs from 5 different tissues revealed significant transitions in cellular architecture and gene regulatory networks at different locations and developmental stages, with hematopoietic stem cells showing progression from cycling to quiescence during ontogeny. The dataset was also useful for understanding aberrant hematopoiesis in comparison to two cancers that present at distinct time points in postnatal life.
Review
Engineering, Biomedical
Chandralekha Chatterjee, Peter Schertl, Miriam Frommer, Anita Ludwig-Husemann, Anna Mohra, Nadine Dilger, Toufik Naolou, Sophia Meermeyer, Timna Claire Bergmann, Alejandro Alonso Calleja, Cornelia Lee-Thedieck
Summary: Artificial niches play a crucial role in regenerative medicine research, providing insights into the biophysical and biochemical processes of hematopoietic stem cells, as well as facilitating HSCs expansion and targeted differentiation. Due to the lack of natural microenvironment, biomaterials are essential in recreating niche environments, offering promising applications in various fields.
ACTA BIOMATERIALIA
(2021)
Article
Biochemistry & Molecular Biology
Terumasa Umemoto, Alban Johansson, Shah Adil Ishtiyaq Ahmad, Michihiro Hashimoto, Sho Kubota, Kenta Kikuchi, Haruki Odaka, Takumi Era, Daisuke Kurotaki, Goro Sashida, Toshio Suda
Summary: By studying the mitochondrial metabolism and ATP citrate lyase (ACLY) in hematopoietic stem cells (HSCs), we have gained new insights into the regulation of HSCs during hematopoietic regeneration.
Article
Multidisciplinary Sciences
Helene Bjoerg Kristensen, Thomas Levin Andersen, Andrea Patriarca, Klaus Kallenbach, Birgit MacDonald, Tanja Sikjaer, Charlotte Ejersted, Jean-Marie Delaisse
Summary: The dormancy and formation of hematopoietic stem cells and progenitors are guided by signals from the bone marrow microenvironment. Research on the microenvironment of murine hematopoietic stem cells is more comprehensive, while knowledge on murine progenitor microenvironments and even more so in humans is lacking. Characterizing these microenvironments is crucial for understanding hematopoiesis and developing treatments for bone marrow malignancies, but challenging due to difficulties in detecting hematopoietic stem cells in the complex bone marrow landscape.
Article
Cell Biology
Zihan Xu, Libing He, Yilin Wu, Lu Yang, Cheng Li, Hong Wu
Summary: Loss of PTEN alters chromatin accessibility and leads to changes in lineage potentials of fetal liver HSCs, with PU.1 playing a critical role in modulating the epigenetic state and lineage plasticity of prepro-B progenitors.
Review
Endocrinology & Metabolism
Ziru Li, Clifford J. Rosen
Summary: Bone marrow adipose tissue (BMAT) is a significant component of the bone marrow space in healthy adults, but its expansion is associated with aging, obesity, anorexia nervosa, and irradiation. While traditionally viewed as a negative component, recent studies have revealed that BMAT can serve as an energy reservoir and also plays a role in regulating bone formation and hematopoiesis. This review summarizes the unique characteristics of BMAT and provides an updated understanding of its physiological roles based on a newly established mouse model.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2023)
Article
Cell Biology
Thomas L. Rothstein, Leonore A. Herzenberg, Nichol E. Holodick, Eliver Ghosn
B-1 CELL DEVELOPMENT AND FUNCTION
(2015)
Article
Cell Biology
Eliver Eid Bou Ghosn, Yang Yang
B-1 CELL DEVELOPMENT AND FUNCTION
(2015)
Article
Multidisciplinary Sciences
Michael Phillip Kurnellas, Eliver Eid Bou Ghosn, Jill M. Schartner, Jeanette Baker, Jesse J. Rothbard, Robert S. Negrin, Leonore A. Herzenberg, C. Garrison Fathman, Lawrence Steinman, Jonathan B. Rothbard
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2015)
Article
Multidisciplinary Sciences
Darya Y. Orlova, Noah Zimmerman, Stephen Meehan, Connor Meehan, Jeffrey Waters, Eliver E. B. Ghosn, Alexander Filatenkov, Gleb A. Kolyagin, Yael Gernez, Shanel Tsuda, Wayne Moore, Richard B. Moss, Leonore A. Herzenberg, Guenther Walther
Article
Cell & Tissue Engineering
Eliver Eid Bou Ghosn, Jeffrey Waters, Megan Phillips, Ryo Yamamoto, Brian R. Long, Yang Yang, Rachel Gerstein, Cheryl A. Stoddart, Hiromitsu Nakauchi, Leonore A. Herzenberg
Article
Allergy
Jin-Young Min, Jayakar V. Nayak, Kathryn E. Hulse, Whitney W. Stevens, Paul A. Raju, Julia H. Huang, Lydia A. Suh, Griet A. Van Roey, James E. Norton, Roderick G. Carter, Caroline P. E. Price, Ava R. Weibman, Ali R. Rashan, Eliver E. Ghosn, Zara M. Patel, Tetsuya Homma, David B. Conley, Kevin C. Welch, Stephanie Shintani-Smith, Anju T. Peters, Leslie C. Grammer, Kathleen E. Harris, Atsushi Kato, Peter H. Hwang, Robert C. Kern, Leonore A. Herzenberg, Robert P. Schleimer, Bruce K. Tan
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2017)
Article
Multidisciplinary Sciences
Darya Y. Orlova, Stephen Meehan, David Parks, Wayne A. Moore, Connor Meehan, Qian Zhao, Eliver E. B. Ghosn, Leonore A. Herzenberg, Guenther Walther
SCIENTIFIC REPORTS
(2018)
Review
Developmental Biology
Eliver Ghosn, Momoko Yoshimoto, Hiromitsu Nakauchi, Irving L. Weissman, Leonore A. Herzenberg
Article
Biology
Stephen Meehan, Gleb A. Kolyagin, David Parks, Justin Youngyunpipatkul, Leonore A. Herzenberg, Guenther Walther, Eliver E. B. Ghosn, Darya Y. Orlova
COMMUNICATIONS BIOLOGY
(2019)
Letter
Multidisciplinary Sciences
Eliver Ghosn, Kenneth Dorshkind, Leonore A. Herzenberg, Nichol Holodick, Aaron Kantor, Encarnacion Montecino-Rodriguez, Thomas L. Rothstein, Gregg J. Silverman, Yang Yang, Mervin C. Yoder, Momoko Yoshimoto
Meeting Abstract
Immunology
Eliver Ghosn, Hedwich Kuipers, Jeffrey Waters, Jasmine Sosa, Megan Phillips, Leonore A. Herzenberg
JOURNAL OF IMMUNOLOGY
(2018)
Meeting Abstract
Immunology
Shu-Chen Hung, Narendiran Rajasekaran, Shoujun Zhu, Zhuoran Ma, Eliver Ghosn, Elizabeth D. Mellins
JOURNAL OF IMMUNOLOGY
(2018)
Meeting Abstract
Immunology
E. Ghosn, J. Waters, M. Phillips, R. Yamamoto, B. Long, Y. Yang, R. Gerstein, C. Stoddart, H. Nakauchi, L. Herzenberg
EUROPEAN JOURNAL OF IMMUNOLOGY
(2016)
Meeting Abstract
Clinical Neurology
Michael P. Kurnellas, Jonathan B. Rothbard, Eliver Ghosn, Lawrence Steinman
MULTIPLE SCLEROSIS JOURNAL
(2016)
Meeting Abstract
Immunology
Eliver Ghosn, Ryo Yamamoto, Megan Phillips, Jeffrey Waters, Yang Yang, Hiromitsu Nakauchi, Leonore Herzenberg
JOURNAL OF IMMUNOLOGY
(2015)