Article
Neurosciences
A. D. Bird, L. H. Deters, H. Cuntz
Summary: The connectivity of cortical microcircuits plays a crucial role in brain function, with the spatial organization of inputs within dendrites impacting synaptic integration. A simple equation allows estimation of potential anatomical contacts between neurons, predicting the mean number of candidate synapses for reconstructed cells. Mature cortical data shows an excess of potential local connections, enabling specific innervation of distinct dendritic compartments.
Article
Immunology
Maarten K. Nijen Twilhaar, Lucas Czentner, Rianne G. Bouma, Katarzyna Olesek, Joanna Grabowska, Aru Zeling Wang, Alsya J. Affandi, Saskia C. Belt, Hakan Kalay, Cornelus F. van Nostrum, Yvette van Kooyk, Gert Storm, Joke M. M. den Haan
Summary: TLR-containing GM3 liposomes are effective vectors to induce DC maturation and T cell priming. Incorporation of PGPC or MDP does not enhance immune responses. These findings provide guidance for the optimal selection of liposomal components to stimulate anti-cancer immune responses.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Virology
Olivia Munoz, Riddhima Banga, Matthieu Perreau
Summary: During the final phase of HIV viral production, nascent HIV virions acquire a portion of the cellular lipid membrane to form an external lipid envelope, potentially incorporating host molecules with retained biological activity, which may affect HIV pathogenesis.
Review
Cell Biology
Sepiso K. Masenga, Bislom C. Mweene, Emmanuel Luwaya, Lweendo Muchaili, Makondo Chona, Annet Kirabo
Summary: The development of antiretroviral drugs (ARVs) is a significant achievement in managing HIV infections by suppressing viral activity and extending lifespan. However, the success of HIV in evading the immune system has hindered the discovery of an effective treatment for four decades. Understanding the molecular interaction between HIV and host cells is crucial for developing preventive and curative therapies. This review highlights the inherent mechanisms of HIV, such as targeting CD4(+) lymphocytes, downregulating MHC class I and II, antigenic variation, and minimizing antibody access, which collectively render the immune system ineffective.
Article
Engineering, Biomedical
Xing Chen, Xiang Ling, Jiaxuan Xia, Ying Zhu, Longlong Zhang, Yuwei He, Anni Wang, Guolong Gu, Bo Yin, Jianxin Wang
Summary: The cytomembrane-derived delivery platform is a promising biomimetic strategy for oncotherapy. By fusing mature dendrosomes with redox-responsive nanoparticles, durable and reliable tumor inhibition can be achieved, along with enhanced immunogenicity and immunocyte differentiation. Combination with aPD-L1 further enhances the antitumor effect.
BIOACTIVE MATERIALS
(2022)
Article
Biochemistry & Molecular Biology
Alfredo Torres, Sheilah Vivanco, Francisca Lavin, Cristian Pereda, Alexey Chernobrovkin, Alejandra Gleisner, Marcela Alcota, Milton Larrondo, Mercedes N. Lopez, Flavio Salazar-Onfray, Roman A. Zubarev, Fermin E. Gonzalez
Summary: Damage-associated molecular patterns (DAMPs) play a critical role in dendritic cells (DCs) ability to trigger a specific and efficient adaptive immune response. This study identified a new inducible DAMP, Haptoglobin (HP), in a heat shock-conditioned melanoma cell lysate. Stimulation with HP enhanced the expression of specific DC maturation markers and pro-inflammatory cytokines, suggesting its important role in DC activation for cancer immunotherapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Virology
Boris Anokhin, Paul Spearman
Summary: The HIV-1 envelope glycoprotein follows a unique pathway to reach the site of particle assembly, involving secretion, endocytosis, and directed sorting. The host factors and pathways regulating Env trafficking are still being explored. Understanding these processes is crucial for developing strategies to prevent viral infection and replication.
Article
Microbiology
Olivia Munoz, Riddhima Banga, Rachel Schelling, Francesco Andrea Procopio, Andrea Mastrangelo, Pauline Nortier, Khalid Ohmiti, Jean Daraspe, Matthias Cavassini, Craig Fenwick, Laurent Perez, Matthieu Perreau
Summary: This study reveals an immunovirological mechanism by which HIV exploits PD-L1 to suppress Tfh-cell function.
Article
Pharmacology & Pharmacy
Yinghua Li, Li Li, Songyi Li, Chaying He
Summary: The study found that mifepristone affects immune homeostasis in the endometrium. Mifepristone can activate dendritic cells and enhance their ability to stimulate T cells. Additionally, mifepristone can alter the balance between Th1 and Th2 cells.
ADVANCED THERAPEUTICS
(2023)
Article
Multidisciplinary Sciences
Olivia Munoz, Riddhima Banga, Rachel Schelling, Francesco Andrea Procopio, Andrea Mastrangelo, Pauline Nortier, Khalid Ohmiti, Jean Daraspe, Matthias Cavassini, Craig Fenwick, Laurent Perez, Matthieu Perreau
Summary: The study revealed that HIV incorporates PD-L1 into its virions actively, impairing Tfh-cell function and reducing the production of broadly neutralizing antibodies by B cells. The functional impairment of Tfh cells due to PD-L1 incorporation into HIV virions may contribute to the limited development of effective BnAbs during HIV infection.
Article
Biochemistry & Molecular Biology
Zhongliu Cao, Yanfeng Wu, Lingling Yu, Lingfeng Zou, Liu Yang, Sijian Lin, Jue Wang, Zhen Yuan, Jianghua Dai
Summary: This study demonstrates that mDC-Exo promotes osteogenic differentiation of BM-MSCs and enhances BM-MSCs-mediated bone regeneration through the transfer of miR-335.
MOLECULAR MEDICINE
(2021)
Article
Cell Biology
Anthony Lefebvre, Camille Trioen, Sarah Renaud, William Laine, Benjamin Hennart, Clement Bouchez, Bertrand Leroux, Delphine Allorge, Jerome Kluza, Elisabeth Werkmeister, Guillaume Paul Grolez, Nadira Delhem, Olivier Morales
Summary: This study found that nasopharyngeal carcinoma-derived small extracellular vesicles (NPCSEVs) have an immunosuppressive impact on the tumor microenvironment, and that this effect is potentially mediated by the galectin-9 (Gal9) they carry.
JOURNAL OF EXTRACELLULAR VESICLES
(2023)
Article
Oncology
Jiachang Wu, Renli Zhao, Jingxing Dai, Guohua Lai, Asmat Ullah Khan, Xiao Yu, Sha Wu, Jun Ouyang, Hongxun Sang
Summary: This study investigated the expression profiles of lncRNAs in exosomes derived from mature and immature dendritic cells, identifying specific lncRNAs involved in immune regulation. Results showed upregulated lncRNAs were associated with immune system process while downregulated lncRNAs were involved in poly(A) RNA binding. The study also suggested a potential role of lncRNAs in modulating immune regulation through TNF signaling and ribosome pathway.
MOLECULAR MEDICINE REPORTS
(2021)
Article
Cell Biology
Heeva Baharlou, Nicolas Canete, Erica E. Vine, Kevin Hu, Di Yuan, Kerrie J. Sandgren, Kirstie M. Bertram, Najla Nasr, Jake W. Rhodes, Martijn P. Gosselink, Angelina Di Re, Faizur Reza, Grahame Ctercteko, Nimalan Pathma-Nathan, Geoff Collins, James Toh, Ellis Patrick, Muzlifah A. Haniffa, Jacob D. Estes, Scott N. Byrne, Anthony L. Cunningham, Andrew N. Harman
Summary: The initial immune response to HIV determines transmission. However, due to technical limitations we still do not have a comparative map of early mucosal transmission events. By combining RNAscope, immunofluorescence, and image analysis tools, the researchers quantified HIV transmission signatures in intact human colorectal explants within 2 hours of topical exposure. They found that HIV enrichment occurs in mucosal dendritic cells (DCs) and submucosal macrophages, but not CD4+ T cells. The researchers also discovered that HIV entry induces recruitment and clustering of target cells, facilitating DC- and macrophage-mediated HIV transfer and enhanced infection of CD4+T cells.
Review
Cell Biology
Sushama Jadhav, Vijay Nema
Summary: HIV-1 triggers activation of chemokine receptors, inflammatory mediators, and excitotoxicity mediated by glutamate receptors, disrupting neuronal and glial functions. Despite widespread use of antiretroviral therapy, brain function decline, mood swings, and abnormal activity continue to be observed, with an increase in HIV-associated neurocognitive disorders.
MEDIATORS OF INFLAMMATION
(2021)
Article
Virology
Caitlin M. Miller, Hisashi Akiyama, Luis M. Agosto, Ann Emery, Chelsea R. Ettinger, Ronald I. Swanstrom, Andrew J. Henderson, Suryaram Gummuluru
JOURNAL OF VIROLOGY
(2017)
Article
Virology
John B. Ruedas, Jason T. Ladner, Chelsea R. Ettinger, Suryaram Gummuluru, Gustavo Palacios, John H. Connor
JOURNAL OF VIROLOGY
(2017)
Article
Virology
Hisashi Akiyama, Nora-Guadalupe Pina Ramirez, Gregory Gibson, Christopher Kline, Simon Watkins, Zandrea Ambrose, Suryaram Gummuluru
JOURNAL OF VIROLOGY
(2017)
Article
Chemistry, Multidisciplinary
Amin Feizpour, David Stelter, Crystal Wong, Hisashi Akiyama, Suryaram Gummuluru, Tom Keyes, Bjorn M. Reinhard
Article
Medicine, Research & Experimental
Victor Pena-Cruz, Luis M. Agosto, Hisashi Akiyama, Alex Olson, Yvetane Moreau, Jean-Robert Larrieux, Andrew Henderson, Suryaram Gummuluru, Manish Sagar
JOURNAL OF CLINICAL INVESTIGATION
(2018)
Article
Multidisciplinary Sciences
Fangda Xu, Asanga Bandara, Hisashi Akiyama, Behnaz Eshaghi, David Stelter, Tom Keyes, John E. Straub, Suryaram Gummuluru, Bjorn M. Reinhard
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Article
Multidisciplinary Sciences
Hisashi Akiyama, Caitlin M. Miller, Chelsea R. Ettinger, Anna C. Belkina, Jennifer E. Snyder-Cappione, Suryaram Gummuluru
NATURE COMMUNICATIONS
(2018)
Article
Multidisciplinary Sciences
Minghua Li, Abdul A. Waheed, Jingyou Yu, Cong Zeng, Hui-Yu Chen, Yi-Min Zheng, Amin Feizpour, Bjorn M. Reinhard, Suryaram Gummuluru, Steven Lin, Eric O. Freed, Shan-Lu Liu
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Article
Cell Biology
Emily L. Morton, Christian Forst, Yue Zheng, Ana B. DePaula-Silva, Nora-Guadalupe P. Ramirez, Vicente Planelles, Ivan D'Orso
Article
Microbiology
Matthew Gagne, Daniel Michaels, Gillian M. Schiralli Lester, Suryaram Gummuluru, Wilson W. Wong, Andrew J. Henderson
Review
Virology
Ashutosh Shukla, Nora-Guadalupe P. Ramirez, Ivan D'Orso
Article
Chemistry, Multidisciplinary
Behnaz Eshaghi, Nourin Alsharif, Xingda An, Hisashi Akiyama, Keith A. Brown, Suryaram Gummuluru, Bjorn M. Reinhard
Article
Virology
Hisashi Akiyama, Sallieu Jalloh, Seonmi Park, Maohua Lei, Gustavo Mostoslavsky, Suryaram Gummuluru
Summary: Persistent HIV icRNA expression in microglia leads to immune activation and inflammation, with control of viral gene expression and nuclear export pathways attenuating the immune response. Novel therapeutic strategies targeting HIV icRNA expression are needed to address HIV-induced neuroinflammation.
JOURNAL OF VIROLOGY
(2021)
Article
Multidisciplinary Sciences
Nora-Guadalupe P. Ramirez, Jeon Lee, Yue Zheng, Lianbo Li, Bryce Dennis, Didi Chen, Ashwini Challa, Vicente Planelles, Kenneth D. Westover, Neal M. Alto, Ivan D'Orso
Summary: This study revealed an undescribed modulator, ADAP1, that influences the fate of HIV-1 provirus. The experimental results showed that ADAP1 can enhance T cell signaling and facilitate the escape of latent HIV-1 by activating the ERK-AP-1 axis.
NATURE COMMUNICATIONS
(2022)
Article
Virology
Emily Cruz-Lorenzo, Nora-Guadalupe P. Ramirez, Jeon Lee, Sonali Pandhe, Lei Wang, Juan Hernandez-Doria, Adam M. Spivak, Vicente Planelles, Tianna Petersen, Mamta K. Jain, Elisabeth D. Martinez, Ivan D'Orso
Summary: This study discovered a small molecule activator that alters the state of CD4(+) T cells to promote transcription and reactivation of latent HIV-1 through a unique mechanism of action. The activator triggered oxidative stress and activated a redox-responsive program involving cell-signaling kinases and atypical transcription factors, resulting in changes in viral transcriptional rewiring.