期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 109, 期 47, 页码 19321-19326出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1208795109
关键词
methanotrophy; biomarker; acetyl-CoA pathway; syntrophy
资金
- Deutsche Forschungsgemeinschaft (through the Research Center/Excellence Cluster MARUM-Center for Marine Environmental Sciences and the graduate school GLOMAR-Globale Change in the Marine Realm)
- European Research Council under European Union's Seventh Framework Programme-Ideas Specific Programme
- ERC [247153]
- Alexander von Humboldt Foundation
- Gott-fried Wilhelm Leibniz Prize
- Max Planck Society
- NSF [OCE-0647633]
- European Research Council (ERC) [247153] Funding Source: European Research Council (ERC)
The methane-rich, hydrothermally heated sediments of the Guaymas Basin are inhabited by thermophilic microorganisms, including anaerobic methane-oxidizing archaea (mainly ANME-1) and sulfate-reducing bacteria (e. g., HotSeep-1 cluster). We studied the microbial carbon flow in ANME-1/HotSeep-1 enrichments in stable-isotope-probing experiments with and without methane. The relative incorporation of C-13 from either dissolved inorganic carbon or methane into lipids revealed that methane-oxidizing archaea assimilated primarily inorganic carbon. This assimilation is strongly accelerated in the presence of methane. Experiments with simultaneous amendments of both C-13-labeled dissolved inorganic carbon and deuterated water provided further insights into production rates of individual lipids derived from members of the methane-oxidizing community as well as their carbon sources used for lipid biosynthesis. In the presence of methane, all prominent lipids carried a dual isotopic signal indicative of their origin from primarily autotrophic microbes. In the absence of methane, archaeal lipid production ceased and bacterial lipid production dropped by 90%; the lipids produced by the residual fraction of the metabolically active bacterial community predominantly carried a heterotrophic signal. Collectively our results strongly suggest that the studied ANME-1 archaea oxidize methane but assimilate inorganic carbon and should thus be classified as methane-oxidizing chemoorganoautotrophs.
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