期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 109, 期 6, 页码 1949-1954出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1105266109
关键词
cell cycle; DNA repair; xeroderma pigmentosa; tricothiodystropy; Cockayne syndrome
资金
- National Institutes of Health (NIH) National Center for Research Resources [RR017573, RR023093]
- National Cancer Institute [T32 CA09151]
- NIH [AI21144, R01 GM0670167, F32-DK080622, P41 RR-01081]
Structures of complete 10-subunit yeast TFIIH and of a nested set of subcomplexes, containing 5, 6, and 7 subunits, have been determined by electron microscopy (EM) and 3D reconstruction. Consistency among all the structures establishes the location of the minimal core subunits (Ssl1, Tfb1, Tfb2, Tfb4, and Tfb5), and additional densities can be specifically attributed to Rad3, Ssl2, and the TFIIK trimer. These results can be further interpreted by placement of previous X-ray structures into the additional densities to give a preliminary picture of the RNA polymerase II preinitiation complex. In this picture, the key catalytic components of TFIIH, the Ssl2 ATPase/helicase and the Kin28 protein kinase are in proximity to their targets, downstream promoter DNA and the RNA polymerase C-terminal domain.
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