Review
Oncology
Christabelle Rajesh, Prakash Radhakrishnan
Summary: Cellular signaling pathways are manipulated during cancer progression, including O-glycosylation which can lead to multiple isoforms of glycoproteins. The tumor microenvironment plays a crucial role in tumor development and metastasis, and the expression of Tn and STn antigens on tumors can regulate immune cell function.
FRONTIERS IN ONCOLOGY
(2023)
Article
Oncology
Xiaochen Zhai, Fengtao You, Shufen Xiang, Licui Jiang, Dan Chen, Yafen Li, Shuangshuang Fan, Zhichao Han, Tingting Zhang, Gangli An, Bozhen Zhang, Yusheng Chen, Huimin Meng, Lin Yang
Summary: The study found that CAR-modified Vγ9Vδ2 T cells have anti-tumor activity against the MUC1-Tn antigen, with even stronger effects than CAR-alpha beta T cells. While CAR-Vγ9Vδ2 T cells have shorter persistence, their function can be improved with continuous stimulation.
AMERICAN JOURNAL OF CANCER RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Patricia Guerrero-Ochoa, Raquel Ibanez-Perez, German Berbegal-Pinilla, Diederich Aguilar, Isabel Marzo, Francisco Corzana, Martha Minjarez-Saenz, Javier Macias-Leon, Blanca Conde, Javier Raso, Ramon Hurtado-Guerrero, Alberto Anel
Summary: Two granulysin-based immunotoxins (SM3GRNLY and AR20.5GRNLY) were generated to target and induce cell death in Tn antigen-expressing tumor cells. The immunotoxins demonstrated stronger cell-killing effects compared to GRNLY alone and induced cell death through apoptotic, necroptotic, and necrotic mechanisms. In a xenograft model, the immunotoxins showed tumor-targeting ability and significantly reduced tumor volume. These findings highlight the potential of these immunotoxins as drugs for Tn-expressing cancers.
Article
Oncology
Gabrielle E. Dombek, Ana Sofia Ore, Jane Cheng, Yasuyuki Matsumoto, Jonathan N. Glickman, Aaron Fleishman, Jamie Heimburg-Molinaro, Vitaliy Y. Poylin, Anne Fabrizio, Thomas Cataldo, Evangelos Messaris, Richard D. Cummings
Summary: This study explored the expression of Tn antigen in colorectal cancer (CRC) and found significantly higher expression in both tumor mucosa and adenocarcinoma compared to normal mucosa. The study also revealed that transitional margin (TM) mucosa showed even higher Tn expression than adenocarcinoma itself. These findings suggest the potential of Tn antigen as a biomarker target in CRC.
Article
Oncology
Mathias Simplicien, Annick Barre, Yamina Benkerrou, Els J. M. Van Damme, Pierre Rouge, Herve Benoist
Summary: The study found that HPA can selectively trigger tumor cell death without affecting healthy cells, making it a promising tool for cancer diagnosis and prognosis.
Article
Engineering, Biomedical
Francisca Diniz, Maria Azevedo, Flavia Sousa, Hugo Osorio, Diana Campos, Paula Sampaio, Joana Gomes, Bruno Sarmento, Celso A. Reis
Summary: Drug delivery using nanoparticles has the potential for cancer therapy, particularly for gastric cancer. This study developed surface-functionalized nanoparticles with specific antibodies, which effectively recognized cancer cells expressing the target antigen under flow conditions. These findings highlight the potential use of such nanoparticles for drug delivery in cancer.
MATERIALS TODAY BIO
(2022)
Article
Medicine, Research & Experimental
Francois Bulteau, Michel Thepaut, Maxime Henry, Amandine Hurbin, Laetitia Vanwonterghem, Corinne Vives, Aline Le Roy, Christine Ebel, Olivier Renaudet, Franck Fieschi, Jean-Luc Coll
Summary: This study found that recombinant MGL fragments can efficiently target Tn-positive human tumors without inducing toxicity, making it a promising candidate for in vivo diagnosis and imaging of human tumors and potentially for therapeutic applications.
MOLECULAR PHARMACEUTICS
(2022)
Article
Engineering, Biomedical
Francisca Diniz, Sofia Lamas, Hugo Osorio, Paulo Aguiar, Daniela Freitas, Fatima Gartner, Bruno Sarmento, Celso A. Reis, Joana Gomes
Summary: This study demonstrates the potential of using nanoparticles targeting STn for delivering FRT as a therapeutic approach in gastric cancer. The nanoparticles effectively reduced tumor growth, cell proliferation, and tumor necrosis in vivo.
ACTA BIOMATERIALIA
(2023)
Article
Biochemistry & Molecular Biology
Valeria da Costa, Karina Marino, Santiago A. Rodriguez-Zraquia, Maria Florencia Festari, Pablo Lores, Monique Costa, Mercedes Landeira, Gabriel A. Rabinovich, Sandra J. van Vliet, Teresa Freire
Summary: Lung cancer is the leading cause of cancer-related deaths worldwide. Aberrant glycosylation in lung tumors leads to the expression of tumor-associated carbohydrate structures, such as the Tn antigen, which can influence immune cell function and molecular mechanisms involved in immune regulation circuits.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Lingqiang Gao, Qinghai Lian, Leilei Ma, Shiwei Su, Menglan Yang, Yuan Fang, Zhongqiu Liu, Xiang Luo, Guochao Liao
Summary: An efficient strategy for constructing RC-529 and its derivatives has been established, with four conjugates of RC-529 derivatives with Tn antigen inducing strong T cell dependent immune responses in mice and showing specific cytotoxicity towards Tn-overexpressing cancer cells. This suggests RC-529 as a potential platform for the development of new vaccine carriers with self-adjuvanting properties for cancer treatment, supported by preliminary structure-activity relationship analysis.
CHINESE CHEMICAL LETTERS
(2021)
Article
Biochemistry & Molecular Biology
Maria Florencia Festari, Valeria da Costa, Santiago A. Rodriguez-Zraquia, Monique Costa, Mercedes Landeira, Pablo Lores, Patricia Solari-Saquieres, M. Gabriela Kramer, Teresa Freire
Summary: The study suggests that the Tn antigen plays a role in breast tumor growth and spreading, promoting lung metastases and an immunoregulatory state.
Article
Biochemistry & Molecular Biology
Song Bai, Hang Gao, Lin-Tong-Qing Tan, Lulu Yao, Xin Meng, Yongzhong Zhang
Summary: In this study, a novel conjugate vaccine Tn-BSA-CTS with chitotriose as built-in adjuvant was synthesized, and the effect of adjuvant chitotriose was evaluated. The immunological evaluations showed that Tn-BSA-CTS could stimulate the highest titers of IgG antibodies (102,400) and significantly enhance both humoral and cellular immunity. These results demonstrate the potential of CTS as a novel vaccine adjuvant and suggest that the covalent linkage of tumor vaccine to CTS could be an effective strategy to enhance the efficacy against cancer.
CARBOHYDRATE RESEARCH
(2023)
Article
Multidisciplinary Sciences
Tung-Shing Mamie Lih, Mingming Dong, Leslie Mangold, Alan Partin, Hui Zhang
Summary: This study investigated the clinical utility of urinary marker panels composed of urinary glycopeptides and/or urinary prostate-specific antigen (PSA) for distinguishing non-aggressive from aggressive prostate cancer. The multi-marker panels showed clinically meaningful results with area under the curve (AUC) ranging from 0.70 to 0.85.
SCIENTIFIC REPORTS
(2022)
Review
Biochemistry & Molecular Biology
Berna Tumoglu, Aidan Keelaghan, Fikri Y. Avci
Summary: Protein-carbohydrate interactions are crucial in maintaining immune homeostasis and regulating immune processes. This review focuses on the immune interactions of macrophage galactose-type lectin (MGL) and Tn carbohydrate antigen, highlighting their role in regulating effector T cells, immune suppression, and pathogen recognition.
Article
Hematology
Marie A. Hollenhorst, Katherine H. Tiemeyer, Keira E. Mahoney, Kazuhiro Aoki, Mayumi Ishihara, Sarah C. Lowery, Valentina Rangel-Angarita, Carolyn R. Bertozzi, Stacy A. Malaker
Summary: This study comprehensively analyzed the glycosylation of platelet GPIbα and identified a diverse repertoire of N- and O-glycans. The findings lay the foundation for further research on the functional and structural roles of GPIbα glycans.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2023)
Article
Biochemical Research Methods
Qing Zhang, Zhonghua Li, Tatiana Chernova, Varma Saikam, Richard Cummings, Xuezheng Song, Tongzhong Ju, David F. Smith, Peng G. Wang
ACS SYNTHETIC BIOLOGY
(2019)
Article
Multidisciplinary Sciences
Lucas Encarnacion-Rivera, Steven Foltz, H. Criss Hartzell, Hyojung Choo
Editorial Material
Biology
Guy M. Benian, Hyojung J. Choo
Article
Chemistry, Analytical
Zhonghua Li, Qing Zhang, David Ashline, Yuyang Zhu, Yi Lasanajak, Tatiana Chernova, Vernon Reinhold, Richard D. Cummings, Peng G. Wang, Tongzhong Ju, David F. Smith, Xuezheng Song
ANALYTICAL CHEMISTRY
(2020)
Article
Biochemistry & Molecular Biology
Yuliang Jiang, Tao Wen, Rui Yan, Suryun Kim, Sean R. Stowell, Wenyi Wang, Yingchun Wang, Guangyu An, Richard D. Cummings, Tongzhong Ju
Article
Multidisciplinary Sciences
Junwei Zeng, Mahmoud Eljalby, Rajindra P. Aryal, Sylvain Lehoux, Kathrin Stavenhagen, Matthew R. Kudelka, Yingchun Wang, Jianmei Wang, Tongzhong Ju, Ulrich H. von Andrian, Richard D. Cummings
NATURE COMMUNICATIONS
(2020)
Article
Multidisciplinary Sciences
Thomas D. Madsen, Lasse H. Hansen, John Hintze, Zilu Ye, Shifa Jebari, Daniel B. Andersen, Hiren J. Joshi, Tongzhong Ju, Jens P. Goetze, Cesar Martin, Mette M. Rosenkilde, Jens J. Holst, Rune E. Kuhre, Christoffer K. Goth, Sergey Y. Vakhrushev, Katrine T. Schjoldager
NATURE COMMUNICATIONS
(2020)
Article
Biochemistry & Molecular Biology
Weiming Ouyang, Tao Xie, Hui Fang, Chunling Gao, Tzanko Stantchev, Kathleen A. Clouse, Kun Yuan, Tongzhong Ju, David M. Frucht
Summary: In this study, the researchers compared the abilities of MERS, SARS CoV-1, and SARS CoV-2 spike proteins to induce cytokine expression in human PBMCs. They found that different vendors and batches of SARS CoV-2 spike proteins exhibited varying patterns of cytokine induction, which correlated with the levels of lipopolysaccharide (LPS) present in the commercial spike protein reagents. Additionally, the researchers discovered that SARS CoV-2 spike protein avidly bound soluble LPS in vitro, suggesting a potential mechanism for promoting pathogenic inflammatory cytokine production in virally infected mucosal tissues.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Steven Foltz, Fang Wu, Nasab Ghazal, Jennifer Q. Kwong, H. Criss Hartzell, Hyojung J. Choo
Summary: Limb-girdle muscular dystrophy R12 (LGMD-R12) is mainly found in males, and female patients are less likely to have severe muscle and/or cardiac pathology. A mouse model of LGMD-R12 showed similar sex differences as observed in humans. Male Ano5-/- mice had elevated serum creatine kinase and defective membrane repair after laser injury, while female Ano5-/- mice were similar to wild type. Both male and female Ano5-/- mice exhibited exercise intolerance, but echocardiography revealed that female mice had features of cardiomyopathy. This suggests a potential link between ANO5 mutations and cardiac disease in humans.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Article
Multidisciplinary Sciences
Junwei Zeng, Rajindra P. Aryal, Kathrin Stavenhagen, Chi Luo, Renyan Liu, Xiaohui Wang, Jiaxuan Chen, Hao Li, Yasuyuki Matsumoto, Yingchun Wang, Jianmei Wang, Tongzhong Ju, Richard D. Cummings
Summary: Deficiency in Cosmc leads to disruption of B cell tolerance, resulting in the development of autoimmune pathologies characterized by elevated levels of self-reactive antibodies, enhanced basal activation, and increased frequency of spontaneous germinal center B cells.
Article
Multidisciplinary Sciences
Alicia M. Matthews, Thomas Biel, Uriel Ortega-Rodriguez, Vincent Falkowski, Xin Bush, Talia Faison, Hang Xie, Cyrus Agarabi, V. Ashutosh Rao, Tongzhong Ju
Summary: This study established CHO cell lines that express ACE2-Fc proteins and partially characterized their physicochemical properties and spike protein binding. The results show that the fusion proteins are heavily N-glycosylated, sensitive to thermal stress, and bind to different spike protein variants with varying affinity. This research provides a proof-of-concept production strategy for ACE2-Fc fusion glycoproteins that could be used in the development of alternative countermeasures against emerging SARS-CoV-2 variants.
Correction
Biochemistry & Molecular Biology
Yasuyuki Matsumoto, Matthew R. Kudelka, Melinda S. Hanes, Sylvain Lehoux, Sucharita Dutta, Mark B. Jones, Kathryn A. Stackhouse, Gabrielle E. Cervoni, Jamie Heimburg-Molinaro, David F. Smith, Tongzhong Ju, Elliot L. Chaikof, Richard D. Cummings
Article
Biochemistry & Molecular Biology
Yasuyuki Matsumoto, Matthew R. Kudelka, Melinda S. Hanes, Sylvain Lehoux, Sucharita Dutta, Mark B. Jones, Kathryn A. Stackhouse, Gabrielle E. Cervoni, Jamie Heimburg-Molinaro, David F. Smith, Tongzhong Ju, Elliot L. Chaikof, Richard D. Cummings
Article
Biochemistry & Molecular Biology
Catharina Steentoft, Zhang Yang, Shengjun Wang, Tongzhong Ju, Malene B. Vester-Christensen, Maria F. Festari, Sarah L. King, Kelley Moremen, Ida S. B. Larsen, Christoffer K. Goth, Katrine T. Schjoldager, Lars Hansen, Eric P. Bennett, Ulla Mandel, Yoshiki Narimatsu
Article
Biotechnology & Applied Microbiology
Hamideh Parhiz, Stephanie A. Ketcham, Guozhang Zou, Bidesh Ghosh, Erica J. Fratz-Berilla, Muhammad Ashraf, Tongzhong Ju, Chikkathur N. Madhavarao
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2019)