Article
Chemistry, Analytical
Menghua Lyu, Xuyang Shi, Xiaopan Liu, Yang Liu, Xijun Zhu, Lijuan Liao, Hongyan Zhao, Na Sun, Shiyu Wang, Linzhe Chen, Linyuan Fan, Qumiao Xu, Qianqian Zhu, Kai Gao, Huaying Chen, Yonggang Zhu, Zida Li, Weijin Guo, Yue Zheng, Ying Gu, Longqi Liu, Meiniang Wang, Ya Liu
Summary: Nanobodies, derived from the serum of Camelidae, offer advantages over conventional antibodies and hold promise for immunotherapy and antibody-drug development. A high-throughput nanobody screening platform is crucial for their rapid development. While droplet-based microfluidic systems have shown improved performance compared to traditional phage display technology, directly applying this technology to nanobody screening faces challenges. This study overcame these obstacles by constructing a eukaryotic display system and designing a signal capture system, resulting in successful screening of target cells expressing anti-PD-L1 nanobodies.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Menghua Lyu, Xuyang Shi, Xiaopan Liu, Yang Liu, Xijun Zhu, Lijuan Liao, Hongyan Zhao, Na Sun, Shiyu Wang, Linzhe Chen, Linyuan Fan, Qumiao Xu, Qianqian Zhu, Kai Gao, Huaying Chen, Yonggang Zhu, Zida Li, Weijin Guo, Yue Zheng, Ying Gu, Longqi Liu, Meiniang Wang, Ya Liu
Summary: Nanobodies, derived from Camelidae serum, have advantages over conventional antibodies and hold promise for immunotherapy and antibody-drug development. A high-throughput nanobody screening platform is crucial. This study developed a eukaryotic display system for nanobody screening and used single-cell transcriptomic profiling to uncover intercellular heterogeneity.
ANALYTICAL CHEMISTRY
(2022)
Article
Oncology
Rui Ma, Ting Lu, Zhenlong Li, Kun-Yu Teng, Anthony G. Mansour, Melissa Yu, Lei Tian, Bo Xu, Shoubao Ma, Jianying Zhang, Tasha Barr, Yong Peng, Michael A. Caligiuri, Jianhua Yu
Summary: The study demonstrated that the combination therapy of an oncolytic virus expressing the IL15/IL15Rα complex and off-the-shelf EGFR-CAR NK cells elicited strong antitumor responses in glioblastoma. This therapy significantly inhibited tumor growth and improved survival rates, offering a promising strategy for the clinical management of this devastating disease.
Article
Cell Biology
Hongqin You, Huifang Zhu, Yajie Zhao, Jindong Guo, Quanli Gao
Summary: This study aimed to identify γδT cells with enhanced responses against ccRCC and found that γδT cells with high TIGIT expression exhibited stronger tumor reactivities and cytotoxicity when challenged by tumor cells.
JOURNAL OF LEUKOCYTE BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Hyeri Park, Jin Seok, Jun Hyeong You, Dae Hyun Lee, Ja-Yun Lim, Gi Jin Kim
Summary: Mesenchymal stem cells (MSCs) are a next-generation treatment for degenerative diseases. This study evaluated the efficacy of cell dose in treating ovarian dysfunction by transplanting placenta-derived mesenchymal stem cells (PD-MSCs) into injured rats. The results showed a decrease in apoptosis and oxidative stress, as well as an increase in follicular development in the transplanted group compared to the non-transplanted group. However, there were no significant differences between low and high dose groups in terms of apoptosis, antioxidant levels, or follicular development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Ravinder Verma, Xiameng Chen, Dazhuan Xin, Zaili Luo, Sean Ogurek, Mei Xin, Rohit Rao, Kalen Berry, Richard Lu
Summary: Intermediate progenitor cells play a crucial role in the development of malignant gliomas.
Editorial Material
Biochemistry & Molecular Biology
Louis Gervais, Allison J. Bardin
Summary: A new study reveals the link between downstream effectors of the epidermal growth factor receptor and stem cell growth through mitochondrial biogenesis and metabolic reprogramming.
Article
Chemistry, Medicinal
Dianlong Jia, Feifei Wang, Yujiao Yang, Ping Hu, Hao Song, Yue Lu, Rui Wang, Guangyong Li, Renmin Liu, Jun Li, Fengjiao Yuan
Summary: In this study, LS-DDP nanoparticles modified with Z(EGFR) molecules successfully targeted EGFR tumor cells in vitro and in vivo, demonstrating excellent anti-tumor effects.
JOURNAL OF PHARMACEUTICAL SCIENCES
(2022)
Article
Cell Biology
Chunxue Zhang, Liqiang Zhou, Zhen Wang, Wenxia Gao, Wei Chen, Huina Zhang, Bo Jing, Xu Zhu, Lei Chen, Changhong Zheng, Kaiyan Shi, Li Wu, Liming Cheng, Kunshan Zhang, Yi Eve Sun
Summary: This study revealed variations in cell proliferation and immune modulatory functions of MSCs from different donors, with IFN-gamma and NF-kappa B signaling being positively associated with immune modulatory function. Activation of these pathways could eradicate donor-dependent variations, but overall transcriptomic differences between donor-derived MSCs still exist.
CELL DEATH & DISEASE
(2021)
Article
Engineering, Biomedical
Xing Liu, Jianxin Sui, Cui Li, Qian Wang, Xudong Peng, Fanyue Meng, Qiang Xu, Nan Jiang, Guiqiu Zhao, Jing Lin
Summary: In this study, a beta-glucan-specific nanobody was developed and loaded with natamycin to effectively treat fungal keratitis. The nanobody exhibited antifungal effects through inhibiting fungal growth, disrupting cell wall and biofilm formation, and reducing adhesion ability to corneal epithelial cells. The conjugation with the nanobody significantly improved the antifungal capacity of natamycin and reduced its toxicity. The nanobody also attenuated fungal virulence and relieved inflammatory responses in fungal keratitis.
ACTA BIOMATERIALIA
(2023)
Article
Multidisciplinary Sciences
Yohei Kitamura, Nobuhiko Kanaya, Susana Moleirinho, Wanlu Du, Clemens Reinshagen, Nada Attia, Agnieszka Bronisz, Esther Revai Lechtich, Hikaru Sasaki, Joana Liliana Mora, Priscilla Kaliopi Brastianos, Jefferey L. Falcone, Aldebaran M. Hofer, Arnaldo Franco, Khalid Shah
Summary: This study developed BLBC-BM mouse models and demonstrated the efficacy of dual-target therapy EVDRL, with the E-V domain enhancing its therapeutic effects. Stem cells secreting EVDRL can alleviate tumor burden and significantly increase survival time in mouse models.
Article
Multidisciplinary Sciences
Michela Consonni, Claudio Garavaglia, Andrea Grilli, Claudia de Lalla, Alessandra Mancino, Lucia Mori, Gennaro De Libero, Daniela Montagna, Monica Casucci, Marta Serafini, Chiara Bonini, Daniel Haussinger, Fabio Ciceri, Massimo Bernardi, Sara Mastaglio, Silvio Bicciato, Paolo Dellabona, Giulia Casorati
Summary: The study demonstrates that engineered human T cells can effectively target leukemia cells expressing CD1c through a specific mLPA-TCR, delaying leukemia progression in mouse models. This approach has the potential to overcome MHC barriers between different donors and recipients, reducing the risk of graft-versus-host disease.
NATURE COMMUNICATIONS
(2021)
Article
Pharmacology & Pharmacy
Guangjian Yang, Yaning Yang, Jiaqi Hu, Haiyan Xu, Shuyang Zhang, Yan Wang
Summary: This study examined the clinical outcomes and molecular structures of EGFR exon 20 insertion mutants in NSCLC patients. The results showed that patients with A763_Y764insFQEA and D770delinsGY had a better response to EGFR TKIs, while those with D770_N771insSVD and V769_D770insASV had a poorer response. These findings have implications for treatment selection in NSCLC patients.
FRONTIERS IN PHARMACOLOGY
(2022)
Review
Cell & Tissue Engineering
Amani Yehya, Joe Youssef, Sana Hachem, Jana Ismael, Wassim Abou-Kheir
Summary: Surgical resection, chemotherapy, and radiation are standard therapies for cancer, but they cannot effectively eliminate drug-resistant cancer stem cells (CSCs). By using patient-derived organoids as preclinical tumor models, we can better identify, isolate, and selectively target these CSCs, improving treatment outcomes and reducing the risk of cancer recurrence.
WORLD JOURNAL OF STEM CELLS
(2023)
Article
Oncology
Krishanthan Vigneswaran, Nathaniel H. Boyd, Se-Yeong Oh, Shoeb Lallani, Andrew Boucher, Stewart G. Neill, Jeffrey J. Olson, Renee D. Read
Summary: The study reveals that YAP and TAZ are highly expressed in EGFR-amplified/mutant human GBMs, leading to the survival and proliferation of tumor cells. The benzoporphyrin derivative verteporfin induces apoptosis of EGFR-amplified/mutant GBM cells and shows significant efficacy in inhibiting tumor growth.
CLINICAL CANCER RESEARCH
(2021)
Article
Biotechnology & Applied Microbiology
Romie Tignat-Perrier, Jeroen A. J. M. van de Water, Dorian Guillemain, Didier Aurelle, Denis Allemand, Christine Ferrier-Pages
Summary: Gorgonians, important habitat-providing species in the Mediterranean Sea, are facing declining populations due to microbial diseases and mass mortality caused by heat waves. This study investigates the biological response of two gorgonian species to thermal stress and found that elevated seawater temperature affects the physiology and bacterial communities of the corals. P. clavata is more sensitive to thermal stress and exhibits greater mortality compared to E. cavolini. The microbiota play a limited role in the thermal acclimation of the corals, but major changes in the microbiota are linked to partial mortality in P. clavata.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Oncology
Jo Sasame, Naoki Ikegaya, Masahito Kawazu, Manabu Natsumeda, Takahiro Hayashi, Masataka Isoda, Kaishi Satomi, Arata Tomiyama, Akito Oshima, Hirokuni Honma, Yohei Miyake, Katsuhiro Takabayashi, Taishi Nakamura, Toshihide Ueno, Yuko Matsushita, Hiromichi Iwashita, Yu Kanemaru, Hidetoshi Murata, Akihide Ryo, Keita Terashima, Shoji Yamanaka, Yukihiko Fujii, Hiroyuki Mano, Takashi Komori, Koichi Ichimura, Daniel P. Cahill, Hiroaki Wakimoto, Tetsuya Yamamoto, Kensuke Tateishi
Summary: The study reveals that the treatment of BRAFV600E-mutant high-grade gliomas can lead to drug resistance due to the deregulation of negative feedback mechanisms, causing the reactivation of the MAPK pathway. However, combination therapy using an HSP90 inhibitor with BRAF or MEK inhibitors can overcome this resistance mechanism and improve the therapeutic effect.
CLINICAL CANCER RESEARCH
(2022)
Review
Biochemical Research Methods
Jeroen A. J. M. van de Water, Romie Tignat-Perrier, Denis Allemand, Christine Ferrier-Pages
Summary: Corals play important ecological and economic roles, providing habitat for species and contributing to coastal protection, fisheries, and tourism. Their biotechnological potential, especially in drug discovery and bioenergy, is increasingly recognized. However, corals are facing threats from climate change and pollution, and researchers are evaluating biotechnological approaches to enhance their resilience.
CURRENT OPINION IN BIOTECHNOLOGY
(2022)
Article
Oncology
Leihao Ren, Lingyang Hua, Zhongyuan Bao, Jiaojiao Deng, Daijun Wang, Jiawei Chen, Hong Chen, Tareq A. Juratli, Hiroaki Wakimoto, Ye Gong
Summary: This study evaluated the clinicopathological characteristics, radiology, and long-term outcomes of microcystic meningiomas (MM) and compared it with other subtypes of meningiomas. The results showed that the progression-free survival of MM was worse than other WHO grade 1 subtypes, but better than atypical meningiomas. Skull base location and higher Ki-67 index were identified as independent negative prognostic factors in MM.
JOURNAL OF NEURO-ONCOLOGY
(2023)
Article
Cell Biology
Yun Zhang, Joana Liu Donaher, Sunny Das, Xin Li, Ferenc Reinhardt, Jordan A. Krall, Arthur W. Lambert, Prathapan Thiru, Heather R. Keys, Mehreen Khan, Matan Hofree, Molly M. Wilson, Ozlem Yedier-Bayram, Nathan A. Lack, Tamer T. Onder, Tugba Bagci-Onder, Michael Tyler, Itay Tirosh, Aviv Regev, Jacqueline A. Lees, Robert A. Weinberg
Summary: Loss of PRC2 or KMT2D-COMPASS enables distinct epithelial-mesenchymal transition (EMT) trajectories in carcinoma cells, with differing effects on metastatic ability. These findings provide insight into how carcinoma cells control their entrance into and residence in different states, and which states are favorable for metastasis.
NATURE CELL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Deepak Bhere, Sung Hugh Choi, Pim van de Donk, David Hope, Kiki Gortzak, Amina Kunnummal, Jasneet Khalsa, Esther Revai Lechtich, Clemens Reinshagen, Victoria Leon, Nabil Nissar, Wenya Linda Bi, Cheng Feng, Hongbin Li, Yu Shrike Zhang, Steven H. Liang, Neil Vasdev, Walid Ibn Essayed, Pablo Valdes Quevedo, Alexandra Golby, Naima Banouni, Anna Palagina, Reza Abdi, Brian Fury, Stelios Smirnakis, Alarice Lowe, Brock Reeve, Arthur Hiller, E. Antonio Chiocca, Glenn Prestwich, Hiroaki Wakimoto, Gerhard Bauer, Khalid Shah
Summary: The authors of this study generated bifunctional mesenchymal stem cells and demonstrated their therapeutic efficacy in treating glioblastoma. This research offers promising potential for cell-based therapies to be used in clinical treatment for highly malignant brain tumors.
NATURE COMMUNICATIONS
(2022)
Article
Oncology
Kazuhide Shimizu, Kaoru Tamura, Shoko Hara, Motoki Inaji, Yoji Tanaka, Daisuke Kobayashi, Takashi Sugawara, Hiroaki Wakimoto, Tadashi Nariai, Kenji Ishii, Ichiro Sakuma, Taketoshi Maehara
Summary: This study aimed to investigate the correlation between preoperative MET-PET signals and intraoperative 5-ALA-induced fluorescence in malignant brain tumor surgery. The results showed that strong tumor fluorescence correlated with high MET-PET uptake and cellular proliferation, providing valuable tumor biology information for surgery in situations where MET-PET is not accessible.
Article
Biochemistry & Molecular Biology
Nareg Pinarbasi-Degirmenci, Ilknur Sur-Erdem, Vuslat Akcay, Yasemin Bolukbasi, Ugur Selek, Ihsan Solaroglu, Tugba Bagci-Onder
Summary: By applying fractionated radiotherapy over a long time and selecting irradiation-survivor glioblastoma cells, clinically relevant irradiation-exposed models were generated. The cells exhibited slower growth, resistance to secondary irradiation, upregulation of DDR-related genes, and better DNA repair capacity. The utility of combining DDR inhibitors and irradiation was demonstrated for both naive and IR-Surv glioblastoma cells, as long as cells are refrained from hypoxic conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Ozlem Yedier-Bayram, Bengul Gokbayrak, Alisan Kayabolen, Ali Cenk Aksu, Ayse Derya Cavga, Ahmet Cingoz, Ezgi Yagmur Kala, Goktug Karabiyik, Rauf Gunsay, Beril Esin, Tunc Morova, Firat Uyulur, Hamzah Syed, Martin Philpott, Adam P. Cribbs, Sonia H. Y. Kung, Nathan A. Lack, Tamer T. Onder, Tugba Bagci-Onder
Summary: This study generated an epigenome-wide CRISPR-Cas9 knockout library (EPIKOL) to investigate the roles of epigenetic modifiers in cancer cells. Through eight screens in different cancer types, novel epigenetic modifiers regulating TNBC and prostate cancer cell fitness were identified. The results demonstrate that EPIKOL can be used to identify essential epigenetic modifiers and discover novel anti-cancer targets.
CELL DEATH & DISEASE
(2022)
Article
Oncology
Lingyang Hua, Leihao Ren, Qian Wu, Jiaojiao Deng, Jiawei Chen, Haixia Cheng, Daijun Wang, Hong Chen, Qing Xie, Hiroaki Wakimoto, Ye Gong
Summary: This study aimed to investigate whether the loss of H3K27me3 could predict the risk of re-recurrence in recurrent meningiomas. The results showed that the loss of H3K27me3 expression was more common in recurrent meningiomas compared to primary meningiomas. Furthermore, the loss of H3K27me3 was associated with an earlier re-recurrence and predicted shorter progression-free survival in recurrent grade 1 and grade 2 meningiomas.
JOURNAL OF NEURO-ONCOLOGY
(2023)
Article
Cell Biology
Kok-Siong Chen, Clemens Reinshagen, Thijs A. Van Schaik, Filippo Rossignoli, Paulo Borges, Natalia Claire Mendonca, Reza Abdi, Brennan Simon, David A. Reardon, Hiroaki Wakimoto, Khalid Shah
Summary: We developed a bifunctional whole cancer cell-based therapeutic that can directly kill tumor cells and stimulate the immune system. By modifying tumor cells using CRISPR-Cas9 and engineering them to release immunomodulatory agents, these therapeutic tumor cells (ThTCs) were able to eliminate glioblastoma tumors in mice by inducing cancer cell apoptosis, down-regulating cancer-associated fibroblast-expressed platelet-derived growth factor receptor beta, and activating antitumor immune cell trafficking and antigen-specific T cell activation signaling. This approach showed efficacy in various cancer models and maintained safety through the use of a double kill-switch.
SCIENCE TRANSLATIONAL MEDICINE
(2023)
Review
Biotechnology & Applied Microbiology
Hirotaka Fudaba, Hiroaki Wakimoto
Summary: Oncolytic viruses (OVs) are innovative therapeutic approach for treating malignant brain tumors, featuring unique mechanisms of action. The recent conditional approval of oncolytic herpes simplex virus G47 Delta as a therapeutic for malignant brain tumors is a significant milestone in the field of neuro-oncology.
EXPERT OPINION ON BIOLOGICAL THERAPY
(2023)
Editorial Material
Oncology
Christian Migliarese, Hiroaki Wakimoto
MOLECULAR THERAPY-ONCOLYTICS
(2023)
Article
Genetics & Heredity
Romie Tignat-Perrier, Jeroen A. J. M. Van de Water, Denis Allemand, Christine Ferrier-Pages
Summary: Marine heat waves (MHWs) have resulted in mass mortality of benthic organisms and loss of biodiversity in shallow waters, including the Mediterranean Sea. The mesophotic zone, less affected by MHWs, may play a crucial role in conserving species like the red coral Corallium rubrum. However, the response of these mesophotic octocoral holobionts to changes in seawater temperature remains limited. In this study, we found that C. rubrum exhibits high tolerance to a range of temperatures (12-21°C) but showed negative responses at extremely high temperatures (24°C), indicating the potential impact of MHWs on mesophotic coralligenous reefs and their dependent biodiversity.
ENVIRONMENTAL MICROBIOME
(2023)
Article
Oncology
Andrea Schmidts, Ambike A. Srivastava, Rishab Ramapriyan, Stefanie R. Bailey, Amanda A. Bouffard, Daniel P. Cahill, Bob S. Carter, William T. Curry, Gavin P. Dunn, Matthew J. Frigault, Elizabeth R. Gerstner, Jack Y. Ghannam, Michael C. Kann, Rebecca C. Larson, Mark B. Leick, Brian Nahed, Leland G. Richardson, Irene Scarfo, Jing Sun, Hiroaki Wakimoto, Marcela Maus, Bryan D. Choi
Summary: TanCART, a novel dual-specific tandem CAR T cell targeting EGFRvIII and IL-13R alpha 2, has shown enhanced cytotoxicity against heterogeneous GBM tumors, including patient-derived xenografts. Dual antigen engagement is necessary for achieving long-term, complete, and durable responses in orthotopic murine models. These findings support the development of multi-specific CAR T cells for GBM and other cancers.
NEURO-ONCOLOGY ADVANCES
(2023)