Article
Multidisciplinary Sciences
Anu Tyagi, Tofayel Ahmed, Shi Jian, Saumya Bajaj, Seow Theng Ong, Stephanie Shee Min Goay, Yue Zhao, Igor Vorobyov, Changlin Tian, K. George Chandy, Shashi Bhushan
Summary: This study reports two structures of the human voltage-gated potassium channel Kv1.3 – apo-Kv1.3 and dalazatide-Kv1.3. Both structures are in an activated state and show remarkable rearrangements in the selectivity filter. In apo-Kv1.3, the aromatic residue Y447 adopts a unique position diverging from other K+ channels, while in dalazatide-Kv1.3, binding of dalazatide narrows the selectivity filter and stabilizes Y447 in a position similar to other K+ channels. These structural changes play a crucial role in Kv1.3's transition into the drug-blocked state.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Microbiology
Sanjay Arvind Desai
Summary: Intracellular malaria parasites activate a channel on the host erythrocyte membrane to acquire essential nutrients. This channel, known as plasmodial surface anion channel (PSAC), allows the uptake of sugars, amino acids, purines, vitamins, and organic cations while excluding small Na+ ions. Understanding the selectivity and conductance properties of PSAC is crucial for further research on this important microbial ion channel.
Article
Neurosciences
I-Shan Chen, Jodene Eldstrom, David Fedida, Yoshihiro Kubo
Summary: G-protein-gated inwardly rectifying K+ (GIRK; Kir3.x) channels play important roles in controlling cell excitation in various organs. Disease-associated mutations in these channels can lead to loss of K+ selectivity, with the G156S mutation showing a unique ion permeation pathway compared to other mutants.
JOURNAL OF PHYSIOLOGY-LONDON
(2022)
Review
Biochemistry & Molecular Biology
Cedric Vallee, Brendan Howlin, Rebecca Lewis
Summary: The Epithelial Sodium Channel/Degenerin (ENaC/DEG) family is a superfamily of sodium-selective channels with high homology in the pore region, playing important physiological roles in various animal species. The G/SxS selectivity filter and other conserved residues are crucial for ion selectivity, with different residues involved in ion permeability depending on the ion type. This review highlights new perspectives for further exploration of ion selectivity mechanisms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Weichao Peng, Shuaihu Yan, Ke Zhou, Hai-Chen Wu, Lei Liu, Yuliang Zhao
Summary: By using a modified density gradient ultracentrifugation method, ultrashort single-walled carbon nanotubes (SWCNTs) with a narrow conductance range can be separated and inserted into lipid bilayers to construct high-resolution nanopore sensors. These SWCNT nanopores exhibit 3-5 times higher ion mobility compared to bulk mobility, and are capable of discriminating homologous and isomeric proteinogenic amino acids, which is challenging for other types of nanopore sensors. These findings hold great importance for the development of protein sequencing technologies.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Medicinal
Chante Muller, Diane L. Lynch, Dow P. Hurst, Patricia H. Reggio
Summary: This study reveals that the endocannabinoid anandamide can activate the TRPV1 channel at a unique peripheral binding site, expanding the knowledge of TRPV1 and aiding in the development of new TRPV1 modulators.
JOURNAL OF CHEMICAL INFORMATION AND MODELING
(2021)
Article
Chemistry, Analytical
Jian Xiu, Guang Wang
Summary: A naphthalene-grafted zirconium-organic framework UiO-66, known as HNA@UiO-66-NH2, was synthesized and demonstrated high selectivity and sensitivity in fluorescence sensing. It exhibited turn-off response to Fe3+ in methanol and turn-on response to ClO4- in ethanol, with obvious changes in fluorescence color and low detection limits. Additionally, it could be easily used for naked-eye detection of Fe3+ and ClO4- in a test paper mode.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Multidisciplinary
Tengfei Yan, Shengda Liu, Cong Li, Jiayun Xu, Shuangjiang Yu, Tingting Wang, Hongcheng Sun, Junqiu Liu
Summary: Artificial ion channels derived from single-chain random heteropolymers (RHPs) with high selectivity and controllable gating behavior have been successfully fabricated. Chemically designed to transport K+ over Na+, these channels exhibit switchable characteristics similar to natural gated protein channels. This work provides insights for future design of novel functional nanochannels.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Multidisciplinary Sciences
Yann Loick Chappe, Sandra Pierredon, Alexandre Joushomme, Pablo Molle, Andre Garenne, Anne Canovi, Solene Barbeau, Florence Poulletier De Gannes, Annabelle Hurtier, Isabelle Lagroye, Thomas Ducret, Jean-Franc Comma Ois Quignard, Vincent Compan, Yann Percherancier
Summary: This study developed highly sensitive bioluminescence resonance energy transfer (BRET) probes to track selective ion permeability in ion channels. The results showed that well-known TRPV1 inhibitors more potently inhibit Ca2+ influx than K+ efflux induced by capsaicin (CAPS). Furthermore, AMG517 was found to be a partial agonist of K+ efflux and triggered TRPV1-dependent cell membrane hyperpolarization. Ligand biases were also observed in other families of cationic channels.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Pharmacology & Pharmacy
Agota Csoti, Rosby del Carmen Najera Meza, Ferenc Bogar, Gabor Tajti, Tibor G. Szanto, Zoltan Varga, Georgina B. Gurrola, Gabor K. Toth, Lourival D. Possani, Gyorgy Panyi
Summary: Kv1.3 K+ channels are important in regulating T cell activation and Ca2+ signaling. The novel peptide toxin sVmKTx derived from V. mexicanus smithi shows increased selectivity for Kv1.3 and potential therapeutic application in autoimmune diseases.
BIOCHEMICAL PHARMACOLOGY
(2022)
Article
Nanoscience & Nanotechnology
Chao Li, Pengxiang Liu, Yi Zhai, Liqian Yao, Hu Lin, Longcheng Gao, Lei Jiang
Summary: The article demonstrates unconventional dual ion selectivity in a bipolar channel membrane, showing that when the membrane meets certain conditions, it can function as a selective layer regardless of which layer faces the ion flux. This discovery can potentially lead to the development of a new generation of ion-selective membranes and other separation membranes.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Multidisciplinary
Qi Xiao, Wei-Wei Haoyang, Tao Lin, Zhan-Ting Li, Dan-Wei Zhang, Jun-Li Hou
Summary: A series of peptide-appended bisresorcinarenes were synthesized, which formed tubular conformation induced by intramolecular hydrogen bonds. These derivatives formed unimolecular artificial transmembrane channels in lipid bilayers for selective transport of monovalent cations, and showed reversible ligand-gating behavior in response to alkyl amine and Cu2+.
CHEMICAL COMMUNICATIONS
(2021)
Article
Engineering, Chemical
Zhongjin He, Ben Corry
Summary: The study reveals that under electric fields, the biomimetic nanopore conducts Na+ preferentially and slows down the passage of Ca2+ by selectively binding it to the carboxylate groups. In strong electric fields, a Cl- concentration polarization layer forms on one side of the nanopore, leading to the formation of stable clusters of Cl- and Ca2+ pairs bound at the carboxylate groups, which severely hinders Cl- transport and occasionally suspends the transport of cations. These findings provide design principles and implications for the fabrication of nanoporous graphene membranes for separation applications, such as heavy metal removal from wastewater.
Article
Chemistry, Multidisciplinary
Yi Shen, Fan Fei, Yulong Zhong, Chunhai Fan, Jielin Sun, Jun Hu, Bing Gong, Daniel M. Czajkowsky, Zhifeng Shao
Summary: Researchers have developed a self-assembling nanopore with a hybrid hydrophilic/hydrophobic lumen that allows for regulated water transport by alkali metal ions. The study demonstrates a non-monotonic dependence of water transport on cation size, with a broad range of water flow observed, from minimal in the presence of sodium ions to levels comparable to a biological water channel in their absence. Molecular dynamics simulations reveal that the sensitivity of water flow is due to the binding of cations within the pore, providing a proof-of-concept for the rational design of controllable synthetic water channels.
ACS CENTRAL SCIENCE
(2021)
Article
Engineering, Chemical
Qing Zhu, Yahua Liu, Peipei Zuo, Yu Dong, Zhengjin Yang, Tongwen Xu
Summary: Researchers have developed a sodium selective isoporous membrane derived from lyotropic liquid crystals, which exhibits excellent Na+ selectivity and ion transport properties. This highly selective membrane could play a crucial role in high precision separation and the design of new generation ion selective membranes.
JOURNAL OF MEMBRANE SCIENCE
(2022)
Review
Neurosciences
Lamyaa Khoubza, Franck C. Chatelain, Sylvain Feliciangeli, Florian Lesage, Delphine Bichet
Summary: Potassium channels play a crucial role in cell excitability and signaling, with heteromerization increasing channel function and signaling diversity. Recent studies have shown evidence of heteromerization between different K-2P subunits, contributing to functional diversity and the importance of this family.
JOURNAL OF PHYSIOLOGY-LONDON
(2021)
Article
Urology & Nephrology
Naomi Issler, Sara Afonso, Irith Weissman, Katrin Jordan, Alberto Cebrian-Serrano, Katrin Meindl, Eileen Dahlke, Konstantin Tziridis, Guanhua Yan, Jose M. Robles-Lopez, Lydia Tabernero, Vaksha Patel, Anne Kesselheim, Enriko D. Klootwijk, Horia C. Stanescu, Simona Dumitriu, Daniela Iancu, Mehmet Tekman, Monika Mozere, Graciana Jaureguiberry, Priya Outtandy, Claire Russell, Anna-Lena Forst, Christina Sterner, Elena-Sofia Heinl, Helga Othmen, Ines Tegtmeier, Markus Reichold, Ina Maria Schiessl, Katharina Limm, Peter Oefner, Ralph Witzgall, Lifei Fu, Franziska Theilig, Achim Schilling, Efrat Shuster Biton, Limor Kalfon, Ayalla Fedida, Elite Arnon-Sheleg, Ofer Ben Izhak, Daniella Magen, Yair Anikster, Holger Schulze, Christine Ziegler, Martin Lowe, Benjamin Davies, Detlef Boeckenhauer, Robert Kleta, Tzipora C. Falik Zaccai, Richard Warth
Summary: This study identified a previously unrecognized autosomal recessive disorder characterized by sensorineural deafness and tubular proteinuria caused by a homozygous missense variant of EHD1. Functional studies in patients and animal models revealed that the mutation impairs receptor-mediated endocytosis in proximal tubules, leading to impaired reabsorption of filtered proteins.
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2022)
Article
Pediatrics
Jacqueline Neubauer, Anna-Lena Forst, Richard Warth, Christian Peter Both, Cordula Haas, Joerg Thomas
Summary: Gene variants involved in respiratory chemoreception may play a role in a minority of SIDS cases, but they are not the dominant factor.
PEDIATRIC RESEARCH
(2022)
Article
Pharmacology & Pharmacy
Frank S. Choveau, Ismail Ben Soussia, Delphine Bichet, Chatelain C. Franck, Sylvain Feliciangeli, Florian Lesage
Summary: This study investigates how the inhibitory potassium channels of the TREK1/TRAAK family are affected by multiple stimuli like temperature, membrane stretch, polyunsaturated fatty acids, and pH. Through pharmacological experiments, it was found that the cytoplasmic domain pCt, arachidonic acid, and extracellular pH converge to the same gate within the channel. Additionally, the study provides evidence that the up and down conformations identified by crystallography do not correspond to the open and closed states of these channels.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Oncology
Barbara Oliveira-Mendes, Sylvain Feliciangeli, Melissa Menard, Frank Chatelain, Malak Alameh, Jerome Montnach, Sebastien Nicolas, Beatrice Ollivier, Julien Barc, Isabelle Baro, Jean-Jacques Schott, Vincent Probst, Florence Kyndt, Isabelle Denjoy, Florian Lesage, Gildas Loussouarn, Michel De Waard
Summary: This study aims to standardize and accelerate the phenotyping process of KCNH2 genetic variants to aid in clinical diagnosis and patient counseling. By developing new biophysical protocols and structural evaluations, three novel pathogenicity indexes were defined.
CLINICAL AND TRANSLATIONAL MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Lamyaa Khoubza, Nicolas Gilbert, Eun-Jin Kim, Franck C. Chatelain, Sylvain Feliciangeli, Sophie Abelanet, Dawon Kang, Florian Lesage, Delphine Bichet
Summary: The study reveals that TALK1, TALK2, and TASK2 are coexpressed in human insulin-secreting pancreatic 8-cells and can form functional heterodimers. The heteromerization of these channels endows TALK2 with sensitivity to extracellular alkalosis and increases the unitary conductance of TALK1 and TALK2.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Arnaud Landra-Willm, Ameya Karapurkar, Alexia Duveau, Anne Amandine Chassot, Lucille Esnault, Gerard Callejo, Marion Bied, Stephanie Hafner, Florian Lesage, Brigitte Wdziekonski, Anne Baron, Pascal Fossat, Laurent Marsollier, Xavier Gasull, Eric Boue-Grabot, Michael A. Kienzler, Guillaume Sandoz
Summary: By manipulating neuronal activity using optogenetics and photopharmacology, researchers have developed a light-activatable molecule called LAKI that can block pain-related potassium channels. This molecule has the potential to be a valuable tool for studying these channels, and it can also be used to remotely control pain in animal models without the need for genetic manipulations or viral infection. This non-invasive and reversible approach has applications in pain research and drug screening.
NATURE COMMUNICATIONS
(2023)
Review
Physiology
Malak Alameh, Barbara Ribeiro Oliveira-Mendes, Florence Kyndt, Jordan Rivron, Isabelle Denjoy, Florian Lesage, Jean-Jacques Schott, Michel De Waard, Gildas Loussouarn
Summary: hERG gene mutations are associated with cardiac rhythmic disorders, but the pathogenicity of most mutations remains unknown. Given the association with sudden death, it is crucial to determine the pathogenicity of these variants. This review highlights the need for comprehensive functional characterization of hERG variants and proposes the development of a standardized protocol for meaningful comparison among variants.
FRONTIERS IN PHYSIOLOGY
(2023)
Letter
Oncology
Barbara B. R. Oliveira-Mendes, Malak Alameh, Jerome Montnach, Beatrice Ollivier, Solene Gibaud, Sylvain Feliciangeli, Florian Lesage, Flavien Charpentier, Gildas Loussouarn, Michel De Waard, Isabelle Baro
CLINICAL AND TRANSLATIONAL MEDICINE
(2023)
Article
Biochemical Research Methods
Kira J. Stanzick, Klaus J. Stark, Mathias Gorski, Johannes Schoedel, Rene Krueger, Florian Kronenberg, Richard Warth, Iris M. Heid, Thomas W. Winkler
Summary: KidneyGPS is a user-friendly web application that combines GWAS and post-GWAS analysis results to provide accessible information to kidney researchers, helping them test hypotheses about kidney physiology and disease etiology in the laboratory.
BMC BIOINFORMATICS
(2023)
Article
Genetics & Heredity
Juilee Rege, Sascha Bandulik, Kazutaka Nanba, Carla Kosmann, Amy R. Blinder, Allein Plain, Pankaj Vats, Chandan Kumar-Sinha, Antonio M. Lerario, Tobias Else, Yuto Yamazaki, Fumitoshi Satoh, Hironobu Sasano, Thomas J. Giordano, Tracy Ann Williams, Martin Reincke, Adina F. Turcu, Aaron M. Udager, Richard Warth, William E. Rainey
Summary: This study identified mutations in the SLC30A1 gene associated with PA. The mutations are located near the zinc-binding site in the coding region of SLC30A1 and may cause abnormal ion transport. PA cases with SLC30A1 mutations showed male dominance and increased levels of aldosterone and 18-oxocortisol.
Article
Cell Biology
Katrin Meindl, Naomi Issler, Sara Afonso, Alberto Cebrian-Serrano, Karin Mueller, Christina Sterner, Helga Othmen, Ines Tegtmeier, Ralph Witzgall, Enriko Klootwijk, Benjamin Davies, Robert Kleta, Richard Warth
Summary: EHD1 plays an important role in spermatogenesis and its mutation can lead to testicular abnormalities and infertility. This study suggests that EHD1 may be an underestimated gene associated with infertility in humans.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biochemical Research Methods
M. Dolores Garcia-Fernandez, Franck C. Chatelain, Hugues Nury, Anna Moroni, Christophe J. Moreau
Summary: This study successfully linked G protein-coupled receptors (GPCRs) to ion channels with different structures and oligomeric states, demonstrating the allosteric regulation of ion channels through their N-terminal domains. Results showed that the ion channels could be modulated by GPCR agonists and antagonists, indicating a generalizable approach for ion channel engineering.
CELL REPORTS METHODS
(2021)
