Article
Biology
C. Maresca, A. Dello Stritto, C. D'Angelo, E. Petti, A. Rizzo, E. Vertecchi, F. Berardinelli, L. Bonanni, A. Sgura, A. Antoccia, G. Graziani, A. Biroccio, E. Salvati
Summary: PARP1 interacts with TRF1 and modifies its DNA affinity, influencing telomere replication and helicase recruitment. This study uncovers a new role for PARP1 as a surveillant of telomere replication, orchestrating protein dynamics at the replication fork.
COMMUNICATIONS BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Masato Mashimo, Momoko Kita, Arina Uno, Moe Nii, Moe Ishihara, Takuya Honda, Yuka Gotoh-Kinoshita, Atsuo Nomura, Hiroyuki Nakamura, Toshihiko Murayama, Ryoichi Kizu, Takeshi Fujii
Summary: Poly(ADP-ribosyl)ation is a post-translational modification process that transfers poly(ADP-ribose) to proteins. TNKS1/2 have been found to play important roles in neuronal development, promoting neurite outgrowth and synapse formation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Konstantin N. Naumenko, Mariya V. Sukhanova, Loic Hamon, Tatyana A. Kurgina, Rashid O. Anarbaev, Aswin Mangerich, David Pastre, Olga I. Lavrik
Summary: Y-box-binding protein 1 (YB-1) is involved in the regulation of gene expression and has been found to play a role in the regulation of PARP1 activity. The C-terminal domain of YB-1 is able to interact with PAR and control its synthesis, providing important insights into the regulation of PARP1 activity.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
T. A. Kurgina, O. I. Lavrik
Summary: Poly(ADP-ribose) (PAR) is a negatively charged polymer composed of ADP-ribose monomers that is synthesized by poly(ADP-ribose)polymerase (PARP) enzymes upon DNA damage. PARP1 and PARP2 are the most well-studied members of the PARP family and play crucial roles in regulating DNA repair. A newly discovered histone PARylation factor (HPF1) modulates the activity of PARP1/2 by forming a transient joint active site with them. The review discusses the discovery and classical mechanism of PARylation in higher eukaryotes, as well as the role of HPF1 in this process.
Review
Cell Biology
Palmiro Poltronieri, Angela Celetti, Luca Palazzo
Summary: NAD(+) is crucial in post-translational modification of proteins and nucleic acids, with its synthesis, degradation, and transport playing a vital role in maintaining optimal NAD(+) levels. The functional connection between NAD(+-utilizing enzymes and mono(ADP-ribosyl)ating enzymes is linked to chronic diseases.
Article
Plant Sciences
Jun Hyeok Kim, Laura J. Bell, Xiao Wang, Rinuckshi Wimalasekera, Hugo P. Bastos, Krystyna A. Kelly, Matthew A. Hannah, Alex A. R. Webb
Summary: Our study found that PARPs and sirtuins do not regulate the circadian oscillator in Arabidopsis, but sirtuin 1 is associated with daytime regulation of gene expression.
PLANT CELL AND ENVIRONMENT
(2021)
Article
Oncology
Emad Matanes, Vanessa M. Lopez-Ozuna, David Octeau, Tahira Baloch, Florentin Racovitan, Amandeep Kaur Dhillon, Roy Kessous, Oded Raban, Liron Kogan, Shannon Salvador, Susie Lau, Walter H. Gotlieb, Amber Yasmeen
Summary: The study showed that PARG inhibition can complement PARP inhibition in the treatment of ovarian cancer, especially in the presence of homologous recombination defects. PARG inhibitor alone or in combination with PARPi or Cisplatin can reduce cell migration and induce cell death.
FRONTIERS IN ONCOLOGY
(2021)
Article
Environmental Sciences
Zhuoying Zeng, Jingjing Lu, Desheng Wu, Ran Zuo, Yuxi Li, Haiyan Huang, Jianhui Yuan, Zhangli Hu
Summary: PARG silencing can inhibit BaP-induced carcinogenesis by downregulating the expression of histone H2B subtypes. High levels of H2B predict poor survival in lung cancer patients, indicating its potential as a new prognostic biomarker. The expression of certain H2B subtypes is positively correlated with PARG expression in lung cancer tissues.
ENVIRONMENTAL TOXICOLOGY
(2021)
Article
Multidisciplinary Sciences
Johannes Gregor Matthias Rack, Qiang Liu, Valentina Zorzini, Jim Voorneveld, Antonio Ariza, Kourosh Honarmand Ebrahimi, Julia M. Reber, Sarah C. Krassnig, Dragana Ahel, Gijsbert A. van der Marel, Aswin Mangerich, James S. O. McCullagh, Dmitri V. Filippov, Ivan Ahel
Summary: Poly(ADP-ribosyl)ation is a versatile posttranslational modification involved in regulating cellular processes. This study focuses on the mechanisms of PAR removal by PARG and ARH3, highlighting their differences in hydrolysis of linear and terminal ADP-ribose linkages.
NATURE COMMUNICATIONS
(2021)
Article
Genetics & Heredity
Tatyana A. Kurgina, Nina A. Moor, Mikhail M. Kutuzov, Olga I. Lavrik
Summary: In this study, the interaction of PARP1 and PARP2 with DNA was compared, revealing their differential affinity and activation ability depending on the DNA structure. The study also highlighted the significant role of HPF1 in regulating the histone modification activity of PARP1 and PARP2.
Article
Medicine, Research & Experimental
Jeffrey Wang, Mohamed A. Ghonim, Salome Ibba, Hanh H. Luu, Yucel Aydin, Peter A. Greer, A. Hamid Boulares
Summary: This study reveals that poly(ADP-ribosyl)ation plays a critical role in protecting STAT6 from degradation, and can be synthetically targeted for degradation by inhibiting PARP-1. Additionally, the study identifies STAT6 as a bonafide substrate for chaperone-mediated autophagy in the human Jurkat cell-line.
JOURNAL OF TRANSLATIONAL MEDICINE
(2022)
Article
Chemistry, Organic
Rui Hagino, Keita Mozaki, Naoko Komura, Akihiro Imamura, Hideharu Ishida, Hiromune Ando, Hide-Nori Tanaka
Summary: This study successfully developed two synthetic approaches to the poly(ADP-ribose) fragment, which plays a key role in DNA repair and cancerization. The first approach involved stepwise assembly of the ribosyl adenosine framework, while the second approach employed direct O-ribofuranosylation of a known riboside acceptor. Both methods showed practicality in terms of LLS steps, pots, and overall yield. Furthermore, the precursor was converted into a conjugation-ready building block for ADP-ribose oligomer synthesis.
EUROPEAN JOURNAL OF ORGANIC CHEMISTRY
(2023)
Editorial Material
Biochemistry & Molecular Biology
Michael S. Cohen
Summary: ADP-ribosylation is a crucial post-translational modification, involving MAR and PAR. Researchers have identified distinct MAR and PAR readers using a chemical biology approach.
Article
Biochemistry & Molecular Biology
Fatima Farhana, Eiko Sakai, Yu Koyanagi, Yu Yamaguchi, Mohammad Ibtehaz Alam, Kuniaki Okamoto, Takayuki Tsukuba
Summary: In this study, the role of Abr protein in osteoclast differentiation and cell morphology was investigated. The results showed that Abr enhances lamellipodia formation and regulates osteoclast differentiation through its interaction with PARG.
MOLECULAR BIOLOGY REPORTS
(2023)
Review
Biochemistry & Molecular Biology
Palmiro Poltronieri, Masanao Miwa, Mitsuko Masutani
Summary: ADP-ribosylation plays a crucial role in post-translational modifications of proteins, involving functions such as DNA repair, transcription, and cell signaling. Enzymes involved in monoADP-ribosylation/polyADP-ribosylation (MAR/PAR) cycling also play important roles in cancers, with potential applications in non-oncological diseases. The field is still actively uncovering novel enzymes at a rapid pace.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Yingbiao Ji, Colin Thomas, Nikita Tulin, Niraj Lodhi, Ernest Boamah, Vladimir Kolenko, Alexei V. Tulin
JOURNAL OF IMMUNOLOGY
(2016)
Article
Medicine, General & Internal
Colin Thomas, Yingbiao Ji, Niraj Lodhi, Elena Kotova, Aaron Dan Pinnola, Konstantin Golovine, Peter Makhov, Kate Pechenkina, Vladimir Kolenko, Alexei V. Tulin
Correction
Neurosciences
Ali Sharma, Shifra Liba Klein, Luendreo Barboza, Niraj Lodhi, Miklos Toth
JOURNAL OF NEUROSCIENCE
(2017)
Article
Biochemistry & Molecular Biology
Niraj Lodhi, Andrew V. Kossenkov, Alexei V. Tulin
NUCLEIC ACIDS RESEARCH
(2014)
Article
Multidisciplinary Sciences
Colin Thomas, Yingbiao Ji, Chao Wu, Haily Datz, Cody Boyle, Brett MacLeod, Shri Patel, Michelle Ampofo, Michelle Currie, Jonathan Harbin, Kate Pechenkina, Niraj Lodhi, Sarah J. Johnson, Alexei V. Tulin
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Article
Dermatology
Jaideep Banerjee, Niraj Lodhi, Bao-Ngoc Nguyen
ADVANCES IN WOUND CARE
(2019)
Article
Immunology
Shanawaz M. Ghouse, Surya K. Vadrevu, Sasikanth Manne, Britney Reese, Jalpa Patel, Bhaumik Patel, Ashok Silwal, Niraj Lodhi, Yvonne Paterson, Sanjay K. Srivastava, Magdalena Karbowniczek, Maciej M. Markiewski
JOURNAL OF IMMUNOLOGY
(2020)
Article
Multidisciplinary Sciences
Guillaume Bordet, Niraj Lodhi, Danping Guo, Andrew Kossenkov, Alexei V. Tulin
SCIENTIFIC REPORTS
(2020)
Review
Biochemistry & Molecular Biology
Niraj Lodhi, Rubi Singh, Satya Prakash Rajput, Quaiser Saquib
Summary: The study highlights the roles of ACE2 and TMPRSS2 genes in host cells and their transcriptional regulation. Furthermore, it discusses the impact of the codon 72 Single Nucleotide Polymorphism (SNP) of p53 gene on the mortality rate of SARS-CoV-2 in different regions, as well as potential emerging therapies using bioengineered bacteriophage, anti-sense, or CRISPR strategies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Rakesh Srivastava, Niraj Lodhi
Summary: Mammalian genomic DNA methylation is an important epigenetic modification that regulates gene expression during development. Aberrant DNA methylation can lead to various inheritable diseases, including cancer. Recent research has uncovered the dynamic regulation of DNA methylation and emphasized the role of PARP1 enzyme in its maintenance.
Article
Biochemistry & Molecular Biology
Niraj Lodhi, Mala Singh, Rakesh Srivastava, Samir Sawant, Rakesh Tuli
Summary: The study aims to reveal the mechanism of transcription initiation of tobacco PR-1a gene in the presence or absence of SA. This is the first study that reports nucleosome and repressor complex maintain the inactivation of gene in the uninduced state and that their disassembling initiates the downstream gene activation process.
MOLECULAR BIOLOGY REPORTS
(2023)
Review
Genetics & Heredity
Rashmi Srivastava, Rubi Singh, Shaurya Jauhari, Niraj Lodhi, Rakesh Srivastava
Summary: Epigenetic modifications are heritable, reversible changes in histones or the DNA that control gene functions. Histone methylation is an important regulatory mechanism for the epigenome, and medications targeting epigenetic regulators have shown therapeutic potential in cancer treatment. This review focuses on the role of histone demethylases in tumor development and modulation, emphasizing molecular mechanisms that control cancer cell progression. The development of new molecular inhibitors targeting histone demethylases is also highlighted.
Article
Oncology
Niraj Lodhi, Poonam Nagpal, Sreeja Sarojini, Michaela Keck, Yuk Ming Chiu, Zeenath Parvez, Laura Adrianzen, K. Stephen Suh
Summary: This study shows that the combination of paclitaxel and radiation has a synthetic lethal effect on melanoma cells, indicating its potential as a melanoma therapy without toxicities or harmful effects on normal primary skin cells.
Review
Oncology
Poonam Nagpal, Dante B. Descalzi-Montoya, Niraj Lodhi
Summary: The tumor microenvironment of cHL differs between adult and pediatric patients, with potential novel therapeutic targets arising from the interplay between H-RS cells and EBV-associated microenvironment. Further research is needed to fully understand the clinical implications of these interactions and their impact on treatment outcomes.
Article
Biochemistry & Molecular Biology
S. Prakash, R. Singh, N. Lodhi
CELLULAR AND MOLECULAR BIOLOGY
(2014)
