Article
Biochemistry & Molecular Biology
David Sychantha, Caitlyn M. Rotondo, Kamaleddin H. M. E. Tehrani, Nathaniel Martin, Gerard D. Wright
Summary: Class B metallo-beta-lactamases (MBLs) are enzymes that confer antibiotic resistance in bacteria by catalyzing the hydrolysis of beta-lactam antibiotics with the help of Zn2+ cofactors. Aspergillomarasmine A (AMA) is a natural inhibitor of class B1 MBLs which selectively removes one catalytic Zn2+ cofactor, causing inactivation of enzymes like NDM-1. The mechanism of action of AMA involves encouraging dissociation of Zn2+ from MBLs, leading to their rapid degradation and improved efficacy as a beta-lactam potentiator, although some MBLs with higher metal affinity show greater tolerance to AMA.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Clare. R. Harwood, David A. Sykes, Bradley L. Hoare, Franziska M. Heydenreich, Romez Uddin, David R. Poyner, Stephen J. Briddon, D. B. Veprintsev
Summary: The study demonstrated the isolation of beta 2-adrenoceptor (beta 2AR) using a polymer, diisobutylene maleic acid (DIBMA), which showed improved functional and thermal stability compared to traditional detergent methods. This unique method offers significant advantages for biophysical studies of beta 2AR.
Review
Microbiology
Gautier M. Courbon, John L. Rubinstein
Summary: ATP synthases utilize the proton motive force generated by the electron transport chain to synthesize ATP through a remarkable rotary catalytic mechanism. Advances in cryoEM have allowed determination of high-resolution structures and revealed the diversity of ATP synthase structures across species. These studies have important implications for therapeutic targeting of ATP synthases in human diseases.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Chemistry, Applied
Lucile Lecas, Vincent Dugas, Claire Demesmay
Summary: Affinity chromatography, often overlooked in receptor/ligand studies, offers new perspectives with its simplicity and ability to provide diverse information. Different immobilization strategies have been developed to encompass a wide range of applications, from ligand-ranking to fragment screening and identification of active components from natural extracts.
SEPARATION AND PURIFICATION REVIEWS
(2021)
Article
Biology
Lan Guan, Parameswaran Hariharan
Summary: Two high-resolution X-ray crystal structures of MelB in complex with substrate analogs reveal the sugar recognition mechanism and cation-binding pocket, advancing the understanding of cooperative binding and symport mechanisms in Na+-coupled MFS transporters.
COMMUNICATIONS BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Nan Wang, Shuo Zhang, Yafei Yuan, Hanwen Xu, Elisabeth Defossa, Hans Matter, Melissa Besenius, Volker Derdau, Matthias Dreyer, Nis Halland, Kaihui Hu He, Stefan Petry, Michael Podeschwa, Norbert Tennagels, Xin Jiang, Nieng Yan
Summary: Human glucose transporters (GLUTs), specifically GLUT1 and GLUT3, are potential targets for anticancer therapy. In this study, an engineered GLUT3 variant was used to identify and understand the inhibitory mechanism of an exofacial GLUT3 inhibitor, SA47. This research provides a foundation for the discovery of exofacial inhibitors of GLUTs for therapeutic development.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Medicinal
David R. Bell, Serena H. Chen
Summary: By using Gaussian process regression, we can accurately predict the impact of point mutations on peptide-MHC class II binding affinity, thus aiding in accelerating the design of antigen-specific immunotherapies.
JOURNAL OF CHEMICAL INFORMATION AND MODELING
(2021)
Article
Biochemistry & Molecular Biology
Li Zhang, Haiyang Xu, Yunshi Zhang, Huan Zhang, Zhan Wang, Pengchao Guo, Ping Zhao
Summary: This study determined the three-dimensional crystal structure of JHAMT3 and its binding properties. The results revealed that JHAMT3 shares a similar structural pattern with functional JHAMT2 and is highly expressed in the silk gland of Bombyx mori larvae. It is suggested that JHAMT3 may function as a binding protein for the regulation of juvenile hormone acid levels.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2022)
Article
Chemistry, Analytical
Madeleine Ramona Volz, Bjoern Moosmann
Summary: This article presents the development of a mass-spectrometry-based binding assay for determining the binding affinities of compounds at the human mu-opioid receptor. The assay has a short runtime, high sensitivity, and produces results consistent with radioactive binding assays. It can be used to determine the receptor binding affinities of newly emerging designer opioids.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Long Li, Jing Tang, Haitao Liu, Yi Qian
Summary: The ionophore properties of four kinds of N-alkyl/aryl ammonium resorcinarenes and extended-resorcinarenes were investigated for the first time to fabricate polymeric membrane electrodes for determination of biologically relevant pyrophosphate (PPi) and lysophosphatidic acid (LPA). The proposed ion selective electrodes showed significant preference for PPi and LPA with significant selectivity pattern differences from the Hofmeister series. Potentiometric sensings of PPi and LPA in mineral water and artificial serum were achieved in low mu M concentration range, demonstrating their promising real-world applications.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Amrinder Singh, Maria A. Martinez-Yamout, Peter E. Wright, H. Jane Dyson
Summary: NF-kappa B is a central mediator in cellular signaling pathways, which remains sequestered in the cytoplasm in complex with I kappa B alpha under resting conditions. Activation of NF-kappa B involves phosphorylation, ubiquitination, and degradation of I kappa B alpha, leading to its translocation to the nucleus. A long noncoding RNA, NKILA, was found to modulate the NF-kappa B signaling circuit by interacting with the NF kappa B-I kappa B alpha complex in the cytoplasm.
Article
Multidisciplinary Sciences
Radoslaw Pluta, Eric Aragon, Nicholas A. Prescott, Lidia Ruiz, Rebeca A. Mees, Blazej Baginski, Julia R. Flood, Pau Martin-Malpartida, Joan Massague, Yael David, Maria J. Macias
Summary: FoxH1 is an essential maternal pioneer factor during embryonic development, and this study reveals the molecular basis for its binding specificity and pioneer factor activity through high-resolution structures of FoxH1 proteins and DNAs.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Edward Jenkins, Markus Korbel, Caitlin O'Brien-Ball, James McColl, Kevin Y. Chen, Mateusz Kotowski, Jane Humphrey, Anna H. Lippert, Heather Brouwer, Ana Mafalda Santos, Steven F. Lee, Simon J. Davis, David Klenerman
Summary: The authors characterize how T cells use microvilli to interact with pMHC complexes, and how this process is regulated by a balance between glycocalyx and CD2. T cells form small contacts with target cells by breaching a glycocalyx barrier using microvilli, stabilized by CD2, allowing sensitive, antigen dependent TCR signaling. The use of microvilli equips T cells with discriminatory receptor signaling.
NATURE COMMUNICATIONS
(2023)
Review
Plant Sciences
Daniel Wipf, Carole Pfister, Arnaud Mounier, Nathalie Leborgne-Castel, Wolf B. Frommer, Pierre-Emmanuel Courty
Summary: Hexoses and disaccharides are essential carbon sources for physiological processes, with sugar transport mediated by transport proteins across different organs and playing key roles in interactions with microbes. Studying sugar transporter interactions can uncover important players in biologically and physiologically relevant processes.
TRENDS IN PLANT SCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Miranda N. Limbach, Aleksandra Antevska, Damilola S. Oluwatoba, Amber L. H. Gray, Xian B. Carroll, Christina M. Hoffmann, Xiaoping Wang, Markus W. Voehler, Carlos A. Steren, Thanh D. Do
Summary: An atomic view of the main aqueous conformation of cyclosporine A (CycA) has been obtained using X-ray and single-crystal neutron diffraction, revealing multiple conformers and providing insight into the membrane permeability and binding of CycA.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Biochemistry & Molecular Biology
Vincent Chaptal, Arnaud Kilburg, David Flot, Benjamin Wiseman, Nushin Aghajari, Jean-Michel Jault, Pierre Falson
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2016)
Article
Biochemistry & Molecular Biology
Shweta Nim, Lucia Gonzalez Lobato, Alexis Moreno, Vincent Chaptal, Manpreet Kaur Rawal, Pierre Falson, Rajendra Prasad
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2016)
Article
Biochemistry & Molecular Biology
Denis Lacabanne, Alons Lends, Clement Danis, Britta Kunert, Marie-Laure Fogeron, Vlastimil Jirasko, Claire Chuilon, Lauriane Lecoq, Cedric Orelle, Vincent Chaptal, Pierre Falson, Jean-Michel Jault, Beat H. Meier, Anja Bockmann
JOURNAL OF BIOMOLECULAR NMR
(2017)
Article
Multidisciplinary Sciences
Xavier Robert, Josiane Kassis-Sahyoun, Nicoletta Ceres, Juliette Martin, Michael R. Sawaya, Randy J. Read, Patrice Gouet, Pierre Falson, Vincent Chaptal
SCIENTIFIC REPORTS
(2017)
Article
Chemistry, Multidisciplinary
Kim-Anh Nguyen, Marine Peuchmaur, Sandrine Magnard, Romain Haudecoeur, Cedric Boyere, Saravanan Mounien, Ikram Benammar, Veronica Zampieri, Sebastien Igonet, Vincent Chaptal, Anass Jawhari, Ahcene Boumendjel, Pierre Falson
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2018)
Article
Multidisciplinary Sciences
Vincent Chaptal, Frederic Delolme, Arnaud Kilburg, Sandrine Magnard, Cedric Montigny, Martin Picard, Charlene Prier, Luca Monticelli, Olivier Bornert, Morgane Agez, Stephanie Ravaud, Cedric Orelle, Renaud Wagner, Anass Jawhari, Isabelle Broutin, Eva Pebay-Peyroula, Jean-Michel Jault, H. Ronald Kaback, Marc le Maire, Pierre Falson
SCIENTIFIC REPORTS
(2017)
Article
Multidisciplinary Sciences
Rachad Nasr, Doriane Lorendeau, Ruttiros Khonkarn, Lauriane Dury, Basile Peres, Ahcene Boumendjel, Jean-Claude Cortay, Pierre Falson, Vincent Chaptal, Helene Baubichon-Cortay
SCIENTIFIC REPORTS
(2020)
Article
Biochemistry & Molecular Biology
Veronica Zampieri, Cecile Hilpert, Melanie Garnier, Yannick Gestin, Sebastien Delolme, Juliette Martin, Pierre Falson, Guillaume Launay, Vincent Chaptal
Summary: The Det.Belt server allows researchers to visually represent membrane proteins wrapped by detergents in 3D, aiding in a better understanding of the interaction between the sample and its environment.
Article
Biochemistry & Molecular Biology
Veronica Zampieri, Alexia Gobet, Xavier Robert, Pierre Falson, Vincent Chaptal
Summary: Researchers have found that amphipathic belts surrounding membrane proteins can be visualized in 3D reconstructions using cryo-EM, and the nature of the amphipathic compound does not influence the size of the belt. These observations illustrate that the observed belt corresponds to the minimum ordered layer around membrane proteins in 3D reconstructions.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2021)
Article
Multidisciplinary Sciences
Vincent Chaptal, Veronica Zampieri, Benjamin Wiseman, Cedric Orelle, Juliette Martin, Kim-Anh Nguyen, Alexia Gobet, Margot Di Cesare, Sandrine Magnard, Waqas Javed, Jad Eid, Arnaud Kilburg, Marine Peuchmaur, Julien Marcoux, Luca Monticelli, Martin Hogbom, Guy Schoehn, Jean-Michel Jault, Ahcene Boumendjel, Pierre Falson
Summary: This study resolved three outward-facing conformations of BmrA, a multidrug ABC transporter, using x-ray crystallography and cryo-electron microscopy. It was found that two R6G molecules bind to the drug-binding cavity, inducing a rearrangement of TM1-2 and highlighting local flexibility. Simulations showed that in the absence of R6G, the cavity quickly closed after drug release, while in the presence of R6G, the cavity remained open.
Article
Biochemistry & Molecular Biology
Alexia Gobet, Veronica Zampieri, Sandrine Magnard, Eva Pebay-Peyroula, Pierre Falson, Vincent Chaptal
Summary: Concentration of pure membrane proteins in detergent solution leads to an increase in detergent concentration, the exact amount of which is unknown. Although commonly observed in membrane protein research labs, this phenomenon has been rarely investigated. In this study, we examined the behavior of detergents mixed with membrane proteins during sample concentration using centrifugal devices. We found that the presence of polymers, including membrane or soluble proteins and polysaccharides, results in detergent over-concentration. The degree of detergent over-concentration depends on the centrifugal force applied. By using a specific dye, we observed the formation of a mesh on the concentrator device. Importantly, reducing the centrifugal speed helps decrease the detergent concentration when mixed with macromolecules, as demonstrated with three different membrane proteins. Overall, these findings highlight the non-Newtonian behavior of detergents and provide a valuable framework for improving biochemical and structural studies of membrane proteins.
Article
Biochemistry & Molecular Biology
Juliette Martin, Xavier Robert, Patrice Gouet, Pierre Falson, Vincent Chaptal
Summary: This article investigates the impact of diffraction anisotropy on membrane proteins and finds that there is no single correlation between anisotropy and resolution limits. It also discovers a peak at 4.9 angstrom resolution in membrane proteins, which is correlated with the collinearity of secondary structures. Furthermore, it shows that the presence of this peak is linked to higher diffraction anisotropy.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2023)
Article
Multidisciplinary Sciences
Xavier Robert, Josiane Kassis-Sahyoun, Nicoletta Ceres, Juliette Martin, Michael R. Sawaya, Randy J. Read, Patrice Gouet, Pierre Falson, Vincent Chaptal
Article
Biochemistry & Molecular Biology
Pratima Baghel, Manpreet Kaur Rawal, Mohammad Firoz Khan, Sobhan Sen, Mohammed Haris Siddiqui, Vincent Chaptal, Pierre Falson, Rajendra Prasad
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2017)
Article
Multidisciplinary Sciences
Vincent Chaptal, Arnaud Kilburg, David Flot, Benjamin Wiseman, Nushin Aghajari, Jean-Michel Jault, Pierre Falson
