Article
Developmental Biology
Xiaorei Sai, Yayoi Ikawa, Hiromi Nishimura, Katsutoshi Mizuno, Eriko Kajikawa, Takanobu A. Katoh, Toshiya Kimura, Hidetaka Shiratori, Katsuyoshi Takaoka, Hiroshi Hamada, Katsura Minegishi
Summary: This study reveals the important roles of microtubules and actomyosin in basal body positioning during left-right symmetry breaking in mouse embryos. The activation of myosin II at the anterior side of node cells before the posterior shift of the basal body suggests its contribution to this process. The asymmetry in the organization of basal-body associated microtubules (baMTs) between the anterior and posterior sides of node cells also plays a crucial role.
Article
Biochemistry & Molecular Biology
Akira Noga, Mao Horii, Yumi Goto, Kiminori Toyooka, Takashi Ishikawa, Masafumi Hirono
Summary: The study reveals the importance of centriolar protein Bld10p in the formation of centriole structural symmetry, particularly in the cartwheel-independent mechanism. Bld10p plays a role in determining the inter-triplet distance and regulating the centriole structure in the absence of the cartwheel.
Article
Biochemistry & Molecular Biology
Wen-Wei Luo, Bin-Jun Chen, Yan-mei Wang, Juan-mei Yang, Xiang Liu, Ya-sheng Yuan, Xi Lin, Fang-lu Chi, Ping Chen, Dong-dong Ren
Summary: The study demonstrates the role of PCP in the nasopharyngeal epithelium, showing consistent orientation of ciliated cells and proteins, with Vangl2 mutation disrupting the orientation of epithelial cells. Basal cells replenishing ciliated cells in a parallel arrangement contribute to the uniform orientation of newly generated ciliated cells.
Review
Biology
Shashank Arora, Mausam Rana, Ananya Sachdev, Jacinta S. D'Souza
Summary: In the past few decades, there has been an increase in research on vertebrate cilia and ciliopathy, with collaborations between basic and clinical scientists. This research focuses on ciliary architecture, composition, evolution, and organelle generation, as well as its biological role. Cilia are important in cell/fluid movement, mating, sensory perception, and development, and defects in cilia are associated with various human diseases. Despite the complex genetic etiology, there have been successful translation of ciliary research into mammalian systems, particularly in humans. This review explores the formation of 9+0 and 9+2 ciliary types, the molecules involved in ciliogenesis, and the regulation of their assembly and disassembly.
JOURNAL OF BIOSCIENCES
(2023)
Review
Cell Biology
K. Bouhouche, M. S. Valentine, P. Le Borgne, M. Lemullois, J. Yano, S. Lodh, A. Nabi, A. M. Tassin, J. L. Van Houten
Summary: Cilia are ubiquitous and highly conserved extensions that endow the cell with motility and sensory functions. Paramecium, a ciliated organism, is a suitable model for studying cilia assembly and function, and can be used to validate candidate genes for ciliopathies.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Kotoku Kawaguchi, Shogo Nakayama, Daichi Saito, Haruka Kogiso, Kasane Yasuoka, Yoshinori Marunaka, Takashi Nakahari, Shinji Asano
Summary: Mucociliary clearance is a major defense mechanism of the airway epithelium, and ezrin plays an important role in regulating the beating of airway ciliary cells by promoting the apical surface localization of β(2)AR.
JOURNAL OF CELL SCIENCE
(2022)
Review
Cell Biology
Jaime V. K. Hibbard, Neftali Vazquez, John B. Wallingford
Summary: Cilia are multifunctional organelles that are crucial in the life cycles of various organisms. Despite our advanced understanding of protein transport within cilia, little is known about how they are transported from their synthesis sites to the ciliary base. This review explores the underappreciated topic of intracellular transport in ciliary cell biology.
JOURNAL OF CELL SCIENCE
(2022)
Article
Developmental Biology
Antoine Donati, Isabelle Anselme, Sylvie Schneider-Maunoury, Christine Vesque
Summary: The study found that in the monociliated epithelium of the embryonic zebrafish floor-plate, basal bodies achieve posterior localization through dynamic movements and contacts with Par3 patches. The enrichment of Par3 plays a key role in controlling basal body positioning, providing new insights into the regulation of basal body positioning downstream of the PCP pathway.
Article
Cell Biology
Xin Sheng, Shuanglin Gao, Yan Sheng, Xiadan Xie, Junhua Wang, Yan He
Summary: The study demonstrates that under low fluid shear stress conditions, Vangl2 plays a critical role in primary cilia assembly by recruiting BBS to promote the apical docking of basal bodies and by restraining Dvl2 phosphorylation to reduce cilia disassembly.
CELL AND TISSUE RESEARCH
(2022)
Review
Cell Biology
Lindsay Rathbun, Coralee A. Everett, Dan T. Bergstralh
Summary: Epithelial tissues play a vital role in the functioning of organs, requiring coordination between cells to maintain distinct surfaces. Understanding how polarity is established and maintained in simpler organisms can provide valuable insights into epithelial tissue morphogenesis.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Jieling Wang, Xin Zhu, Zhengmao Wang, Xuecheng Li, Hui Tao, Junmin Pan
Summary: The machinery of intraflagellar transport (IFT) consists of IFT motors and cargo adaptors, including IFT-A and IFT-B complexes. This study reveals the role of IFT38 in the regulation of anterograde IFT and retrograde trafficking of BBSome. The stability of IFT-B1 and IFT-B2 is mutually dependent and mediated by the connecting tetramer IFT38/5788/52. The findings suggest a modular pathway for IFT-B assembly.
Article
Cell Biology
Koshi Kunimoto, Alexis T. Weiner, Jeffrey D. Axelrod, Eszter K. Vladar
Summary: Planar cell polarity signaling regulates cell polarization within and between cells, and is crucial for effective mucociliary clearance. In the mouse airway epithelium, the core protein Prickle2 is only present in multiciliated cells and has modest effects on ciliary polarity. Our study demonstrates that Prickle1 is present in both multiciliated and nonciliated airway epithelial cell types, exhibits different spatial and temporal expression dynamics compared to Prickle2, and has a stronger ciliary polarity phenotype. Additionally, Prickle1 and Prickle2 mutants genetically interact.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Jaroslav Ferenc, Panagiotis Papasaikas, Jacqueline Ferralli, Yukio Nakamura, Sebastien Smallwood, Charisios D. Tsiairis
Summary: The study demonstrates the importance of tissue stretching during inflation for the appearance of the head organizer Wnt3 in Hydra tissue spheroids, with Wnt3 overexpression enabling successful regeneration in the absence of mechanical stimulation. This highlights the significance of mechanochemical feedback loops for patterning epithelial lumens.
Review
Cell Biology
Jia Chen, Daniel St Johnston
Summary: This article reviews the current understanding of cell polarization in different stages (embryonic, larval, pupal, and adult) of Drosophila midgut development, and discusses the unresolved mechanisms that control changes in cell arrangements, cell shape, and cell polarity during midgut development.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Jinqi Ren, Dong Li, Juyuan Liu, Hao Liu, Xiumin Yan, Xueliang Zhu, Wei Feng
Summary: This study reveals the molecular mechanism of how calmodulin-regulated spectrin-associated proteins (Camsaps) bind to WD40-repeat 47 (Wdr47) to generate axonemal central-pair microtubules (CP-MTs) in mammalian multicilia. The researchers determine the structures of Wdr47 and Camsap3 in complex and identify the key interaction sites. Disrupting this interaction impairs the formation of CP-MTs and ciliary beat in ependymal cells. These findings provide new insights into the regulation of multicilia beating.
Review
Cell Biology
Miquel Sureda-Gomez, Teresa Adell
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2019)
Article
Cell Biology
Maria Almuedo-Castillo, Alexander Blaessle, David Moersdorf, Luciano Marcon, Gary H. Soh, Katherine W. Rogers, Alexander F. Schier, Patrick Mueller
NATURE CELL BIOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Nidia de Sousa, Gustavo Rodriguez-Esteban, Ivan Colage, Paolo D'Ambrosio, Jack J. W. A. van Loon, Emili Salo, Teresa Adell, Gennaro Auletta
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2019)
Article
Developmental Biology
Javier Vazquez-Marin, Jose Arturo Gutierrez-Triana, Maria Almuedo-Castillo, Lorena Buono, Jose Luis Gomez-Skarmeta, Juan Luis Mateo, Joachim Wittbrodt, Juan Ramon Martinez-Morales
Review
Clinical Neurology
Marcos J. Cardozo, Maria Almuedo-Castillo, Paola Bovolenta
Article
Developmental Biology
Eudald Pascual-Carreras, Marta Marin-Barba, Carlos Herrera-Ubeda, Daniel Font-Martin, Kay Eckelt, Nidia de Sousa, Jordi Garcia-Fernandez, Emili Salo, Teresa Adell
Article
Multidisciplinary Sciences
Nidia de Sousa, Marcello Caporicci, Jeroen Vandersteen, Jose Ignacio Rojo-Laguna, Emili Salo, Teresa Adell, Gennaro Auletta, Jack J. W. A. van Loon
Article
Genetics & Heredity
Eudald Pascual-Carreras, Miquel Sureda-Gomez, Ramon Barrull-Mascaro, Natalia Jorda, Maria Gelabert, Pablo Coronel-Cordoba, Emili Salo, Teresa Adell
Summary: Organizers are groups of cells that secrete proteins to determine the fate of surrounding cells, crucial for tissue patterning during embryogenesis. While they are also involved in adult development in species with strong regenerative abilities like planarians, their mechanisms are not fully understood. Studies have shown that differential activation of the WNT/beta-catenin signal is key in establishing organizers in wounds, with further research identifying specific receptors involved in this process. This new knowledge contributes to a better understanding of how organizers signal to cells and coordinate the growth and patterning required for organ and tissue formation.
Article
Multidisciplinary Sciences
Martin Franke, Elisa De la Calle-Mustienes, Ana Neto, Maria Almuedo-Castillo, Lbai Irastorza-Azcarate, Rafael D. Acemel, Juan J. Tena, Jose M. Santos-Pereira, Jose L. Gomez-Skarmeta
Summary: CTCF plays a critical role in animal development by regulating three-dimensional chromatin structure and enhancer-promoter interactions, essential for controlling the expression of thousands of genes. The architectural protein CTCF mediates chromatin looping and is enriched at the boundaries of topologically associating domains (TADs), impacting gene regulation during development. Loss of CTCF leads to disrupted chromatin structure and affects the expression of developmental regulators, highlighting its essential role in providing structural context for enhancer-promoter interactions.
NATURE COMMUNICATIONS
(2021)
Correction
Multidisciplinary Sciences
Lorena Buono, Jorge Corbacho, Silvia Naranjo, Maria Almuedo-Castillo, Tania Moreno-Marmol, Berta de la Cerda, Estefania Sanabria-Reinoso, Rocio Polvillo, Francisco-Javier Diaz-Corrales, Ozren Bogdanovic, Paola Bovolenta, Juan-Ramon Martinez-Morales
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Lorena Buono, Jorge Corbacho, Silvia Naranjo, Maria Almuedo-Castillo, Tania Moreno-Marmol, Berta de la Cerda, Estefania Sanbria-Reinoso, Rocio Polvillo, Francisco-Javier Diaz-Corrales, Ozren Bogdanovic, Paola Bovolenta, Juan-Ramon Martinez-Morales
Summary: The formation of sight relies on the tight cooperation between neural retina and retinal-pigmented epithelium, which are derived from common progenitors through bifurcation. Transcriptome dynamics and chromatin accessibility in zebrafish reveal network redundancy and context-dependent transcription factor activity.
NATURE COMMUNICATIONS
(2021)
Article
Biology
Hannes Preiss, Anna C. Koegler, David Moersdorf, Daniel Capek, Gary H. Soh, Katherine W. Rogers, Hernan Morales-Navarrete, Maria Almuedo-Castillo, Patrick Mueller
Summary: During vertebrate embryogenesis, the distribution of Nodal signals is influenced by receptor binding, which provides a mechanism for spatial restriction of Nodal signaling. This study characterized different Types of Activin receptors and discovered that they function partly redundantly and partially independently of Nodal to pattern the early zebrafish embryo. The findings also suggest that feedback-regulated receptors and co-receptors can directly influence the diffusion and distribution of Nodals.
Article
Multidisciplinary Sciences
Eudald Pascual-Carreras, Marta Marin-Barba, Sergio Castillo-Lara, Pablo Coronel-Cordoba, Marta Silvia Magri, Grant N. Wheeler, Jose Luis Gomez-Skarmeta, Josep F. Abril, Emili Salo, Teresa Adell
Summary: Any planarian fragment can regenerate the missing head and tail properly by activating the Wnt/beta-catenin signaling pathway, which changes the chromatin accessibility of the wound cells. The determination of missing tissue identity relies on pre-existing tissue. Planarians are perfect organisms for studying this mechanism, as the same group of cells can regenerate a head or a tail depending on the injured body part. The differential activation of the Wnt/beta-catenin signal after amputation specifies anterior or posterior identity. The chromatin accessibility of wound cells shifts according to the polarity of pre-existing tissue in a Wnt/beta-catenin-dependent manner within 12 hours.
NATURE COMMUNICATIONS
(2023)
Article
Biology
Nidia de Sousa, Teresa Adell
Article
Biology
Nidia de Sousa, Teresa Adell