Article
Chemistry, Physical
Francisco J. Solis, Monica Olvera de la Cruz
Summary: This article discusses the interactions between charged particles and induced polarization charge on solid-liquid or liquid-liquid interfaces with dielectric contrast, as well as the self-energy of charged particles near rough surfaces. Results show that the local convexity and concavity of the surface affect the repulsion of ions.
JOURNAL OF CHEMICAL PHYSICS
(2021)
Article
Multidisciplinary Sciences
Sourav Maity, Gianluca Trinco, Pedro Buzon, Zaid R. Anshari, Noriyuki Kodera, Kien Xuan Ngo, Toshio Ando, Dirk J. Slotboom, Wouter H. Roos
Summary: This study used high-speed atomic force microscopy to visualize the transport cycle of the CitS protein in real time. It revealed the presence of three distinguishable states and independent movement of the protein subunits. These findings provide insights into the transport mechanism of the CitS protein.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Devin T. Edwards, Marc-Andre Leblanc, Thomas T. Perkins
Summary: Single-molecule force spectroscopy was used to study protein folding dynamics, revealing intrinsic changes in protein folding dynamics and transition state height under different pH conditions. Despite differences in destabilization mechanisms, constant-force landscapes showed minimal differences in transition state height, while force-dependent rates increased as pH decreased. This study provides insights for future AFM-based studies of mechanoresponsive proteins.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Chemistry, Physical
Denis Scaini, Fabio Biscarini, Loredana Casalis, Cristiano Albonetti
Summary: Adjacent patches of alkanethiol molecules with chain lengths ranging from 11 to 15 carbon atoms were fabricated using nanografting within a Self-Assembled Monolayer matrix. The structural and electronic properties of these molecules were investigated using Atomic Force Microscopy and Electrostatic Force Microscopy, highlighting the significant influence of substrate roughness. The results revealed an odd-even dependence of local stiffness on alkyl chain length based on topographic phase signal, and an asymmetric parabolic trend of conformational order versus the alkyl chain length induced by substrate roughness based on electrostatic force signal.
CHEMICAL PHYSICS LETTERS
(2022)
Article
Chemistry, Multidisciplinary
Binyu Zhao, Elmar Bonaccurso, Guenter K. Auernhammer, Longquan Chen
Summary: Capillarity and elasticity play crucial roles in determining elastocapillary deformations on soft solids induced by nanodroplets. Experimental observations reveal that the substrate's capillarity dominates deformations when the elastocapillary length is larger than the droplet contact radius. This study provides insights into nanoscale elastocapillarity and has the potential to advance future research in solid mechanics.
Article
Chemistry, Multidisciplinary
Haipei Liu, Zhaowei Liu, Mariana Sa Santos, Michael A. A. Nash
Summary: Single-molecule force spectroscopy (SMFS) is a powerful tool for studying protein folding and mechanical properties. However, the traditional method of immobilization using lysine residues results in a heterogeneous distribution of tether positions. In this study, we compared lysine-based immobilization to site-specific immobilization using genetically encoded peptide tags (ybbR). Our results showed that lysine-based immobilization led to signal deterioration and incorrect classification of unfolding pathways. We also developed a mixed immobilization approach that partially recovered specific signals.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Multidisciplinary
Emanuela Schiliro, Raffaella Lo Nigro, Salvatore E. Panasci, Simonpietro Agnello, Marco Cannas, Franco M. Gelardi, Fabrizio Roccaforte, Filippo Giannazzo
Summary: The authors demonstrated the growth of highly homogeneous and ultrathin Al2O3 films with good insulating properties on monolayer MoS2 exfoliated on gold using atomic layer deposition. The study showed that the nucleation process of the high-k film is better on the 1L MoS2/Au system compared to common insulating substrates, and the density of Al2O3 on bilayer MoS2 is significantly reduced. Raman and photoluminescence spectroscopy indicated p-type doping and tensile strain in the MoS2 induced by the Au substrate after Al2O3 deposition.
ADVANCED MATERIALS INTERFACES
(2021)
Article
Biochemistry & Molecular Biology
Ying Zhuang, Yihong Huang, Zijian He, Tianqi Liu, Xuefei Yu, Sherman Xuegang Xin
Summary: This study found that the perimeters and areas of SiHa cells grown on substrates of different stiffness exhibited significant differences, with cells on stiff substrates having higher Young's modulus. The adhesion force and binding probabilities of SiHa cells both increased with increasing substrate strength.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2022)
Article
Nanoscience & Nanotechnology
Keivan Asadi, Junghoon Yeom, Hanna Cho
Summary: Investigating internal resonance (IR) mechanisms in micro/nanoresonators reveals that intermodal coupling between second and third flexural modes in asymmetric structures provides an optimal condition for strong IR, with high energy transfer to the resonated mode. This study introduces design strategies that can be easily integrated into typical micro/nanoelectromechanical systems, offering potential for paradigm-shifting applications in micro/nanosystems.
MICROSYSTEMS & NANOENGINEERING
(2021)
Article
Chemistry, Physical
S. Mendoza-Rincon, M. S. Ospina-Arroyave, D. F. Arias Mateus, D. Escobar-Rincon, E. Restrepo-Parra
Summary: The roughness scaling behavior of Zr thin films deposited by DC magnetron sputtering was studied using atomic force microscopy scans, showing that the growth exponent increases with rotation speed, resulting in mound-like growth with reduced shadowing effect. The increase in beta was attributed to the remission process, where incoming atoms lose more kinetic energy when colliding with a substrate in motion, leading to an increased sticking coefficient.
APPLIED SURFACE SCIENCE
(2021)
Editorial Material
Multidisciplinary Sciences
Telmo O. Paiva, Albertus Viljoen, Yves F. Dufrene
Summary: Advancements in atomic force microscopy (AFM) techniques and methodologies in microbiology have enhanced our understanding of microbial cell surfaces. Recent studies have shown that AFM imaging of cells and membranes at or near molecular resolution enables detailed visualization of membrane-drug interactions.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Multidisciplinary
Hai Lei, Junsheng Zhang, Ying Li, Xin Wang, Meng Qin, Wei Wang, Yi Cao
Summary: In this study, a histidine-specific phosphorylation strategy was developed to covalently anchor proteins to an atomic force microscopy. This method improved the data quality of single-molecule force spectroscopy experiments and was successfully applied to investigate the mechanical stability and unfolding process of proteins.
Article
Chemistry, Physical
Carolina Paba, Virginia Dorigo, Beatrice Senigagliesi, Nicola Tormena, Pietro Parisse, Kislon Voitchovsky, Loredana Casalis
Summary: This study investigates the interaction between small extracellular vesicles (sEVs) released from triple-negative breast cancer cells and synthetic model membranes. The results show that the fluidity of the membranes plays a crucial role in the uptake of sEVs, with stronger interaction and fusion occurring in less fluid regions.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2023)
Article
Materials Science, Multidisciplinary
B. Molaei, M. Kolahdoozan
Summary: This paper investigates the effect of silver and gold metal coating in various thicknesses on the adhesion force of silicon surfaces. It finds that coatings of both metals with different thicknesses can reduce the adhesion force, with gold being more effective than silver. Gold can be a better option to reduce adhesion force, especially in applications such as micro assembly.
JOURNAL OF MATERIALS ENGINEERING AND PERFORMANCE
(2023)
Article
Physics, Applied
Shiquan Lin, Zhong Lin Wang
Summary: Inspired by TENG, scanning TENG is proposed for local surface charge density measurement using atomic force microscopy. The technique taps a conductive tip above a charged dielectric surface to induce an AC, with Fourier analysis showing a linear relation to surface charge density. Results demonstrate its power in probing nanoscale charge transfer in contact-electrification.
APPLIED PHYSICS LETTERS
(2021)
Article
Biochemistry & Molecular Biology
Noah Ritzmann, Selen Manioglu, Sebastian Hiller, Daniel J. Mueller
Summary: The 8-barrel assembly machinery (BAM) complex is important for inserting and folding outer membrane proteins in Escherichia coli. A natural antibiotic compound called darobactin inhibits the central unit, BamA. Using dynamic single-molecule force spectroscopy, researchers were able to study the structure-function relationship of BamA and its inhibition by darobactin.
Article
Multidisciplinary Sciences
Elisabeth Lambert, Ahmad Reza Mehdipour, Alexander Schmidt, Gerhard Hummer, Camilo Perez
Summary: LtaA is a proton-dependent MFS lipid transporter that catalyzes the translocation of glycolipids through a 'trap-and-flip' mechanism. LtaA has asymmetric lateral openings and its entire amphipathic central cavity can bind to lipids, while its hydrophilic pocket determines substrate specificity.
NATURE COMMUNICATIONS
(2022)
Article
Neurosciences
Prabhjot Dhami, Lena C. Quilty, Benjamin Schwartzmann, Rudolf Uher, Timothy A. Allen, Stefan Kloiber, Raymond W. Lam, Glenda MacQueen, Benicio N. Frey, Roumen Milev, Daniel J. Mueller, Stephen C. Strother, Pierre Blier, Claudio N. Soares, Sagar V. Parikh, Gustavo Turecki, Jane A. Foster, Susan Rotzinger, Sidney H. Kennedy, Faranak Farzan
Summary: The neurobiological correlates of response inhibition can predict the response of patients with major depressive disorder (MDD) to pharmacological and cognitive behavioral therapy treatment. The integrity of response inhibition may be crucial for the success of treatment for MDD. Electrophysiological correlates of response inhibition may serve as a general prognostic marker for treatment response in MDD.
BIOLOGICAL PSYCHIATRY-COGNITIVE NEUROSCIENCE AND NEUROIMAGING
(2023)
Article
Multidisciplinary Sciences
Stefania A. Mari, Kristyna Pluhackova, Joka Pipercevic, Matthew Leipner, Sebastian Hiller, Andreas Engel, Daniel J. Mueller
Summary: Gasdermin-A3 pore formation propagates along diverse pathways, and the assembly and pore-forming mechanisms have been characterized using high-resolution time-lapse atomic force microscopy. The results reveal the role of amphiphilic beta-hairpins and structurally adapting hydrophilic head domains in stabilizing variable oligomeric conformations and opening the pore.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Tetiana Serdiuk, Moutusi Manna, Cheng Zhang, Stefania A. Mari, Waldemar Kulig, Kristyna Pluhackova, Brian K. Kobilka, Ilpo Vattulainen, Daniel J. Muller
Summary: Cholesterol interacts with G protein-coupled receptors in cell membranes, modulating their assembly, stability, and conformation. This study shows that the cholesterol analog CHS can nonlinearly stabilize different structural regions of GPCRs and affect their functionality, with the strongest effects observed at physiological temperature.
Article
Chemistry, Multidisciplinary
Ilaria Incaviglia, Sophie Herzog, Gotthold Flaeschner, Nico Strohmeyer, Enrico Tosoratti, Daniel J. Mueller
Summary: Researchers design photothermally actuated microcantilevers to optimize the accuracy of cell mass measurements. By reducing the inertial mass of the microcantilever using a focused ion beam, they considerably increase its mass sensitivity. The improved microcantilevers allow for more accurate monitoring of cell mass and restrict cell migration, reducing measurement errors.
Article
Biochemistry & Molecular Biology
Nina Blaimschein, Parameswaran Hariharan, Selen Manioglu, Lan Guan, Daniel J. Muller
Summary: In this study, single-molecule force spectroscopy was used to investigate the substrate-induced structural changes of MelB from Salmonella typhimurium. The results showed that in the absence of substrate, MelB equally populated two different states, with one state showing higher mechanical structural stability. However, in the presence of melibiose or a coupling Na+-cation, MelB increasingly populated a mechanically less stable state. In the presence of both substrate and co-substrate, this mechanically less stable state of MelB was predominant. These findings provide mechanistic insights into the symport action of galactosides and cations catalyzed by MelB.
Article
Biochemistry & Molecular Biology
Vikrant Minhas, Arnau Domenech, Dimitra Synefiaridou, Daniel Straume, Max Brendel, Gonzalo Cebrero, Xue Liu, Charlotte Costa, Mara Baldry, Jean-Claude Sirard, Camilo Perez, Nicolas Gisch, Sven Hammerschmidt, Leiv Sigve Havarstein, Jan-Willem Veening
Summary: Competence development in Streptococcus pneumoniae controls genetic transformation, biofilm formation, and virulence. During competence, the genes involved in teichoic acid biosynthesis are essential. LytR and ComM play crucial roles in WTA synthesis and immunity against CbpD. Moreover, key virulence factors PspA and PspC become more surface-exposed at midcell during competence, in a CbpD-dependent manner.
Article
Biochemistry & Molecular Biology
Hella Baumann, Melanie Schwingel, Marcello Sestu, Anna Burcza, Susanna Marg, Wolfgang Ziegler, Anna V. V. Taubenberger, Daniel J. J. Muller, Martin Bastmeyer, Clemens M. M. Franz
Summary: This study investigated the role of vinculin in the reinforcement of nascent adhesions between cells and fibronectin or vitronectin. The researchers found that vinculin is dispensable for adhesion initiation but is crucial for adhesion strength and traction after 60 to 120 seconds. Re-expression of full-length vinculin or a constitutively active vinculin mutant restored adhesion and traction, while vinculin with specific mutations was ineffective.
JOURNAL OF MOLECULAR RECOGNITION
(2023)
Article
Multidisciplinary Sciences
Morris Degen, Jose Carlos Santos, Kristyna Pluhackova, Gonzalo Cebrero, Saray Ramos, Gytis Jankevicius, Ella Hartenian, Undina Guillerm, Stefania A. Mari, Bastian Kohl, Daniel J. Mueller, Paul Schanda, Timm Maier, Camilo Perez, Christian Sieben, Petr Broz, Sebastian Hiller
Summary: Eukaryotic cells can undergo different forms of programmed cell death, many of which culminate in plasma membrane rupture. The protein ninjurin-1 (NINJ1) mediates the active process of membrane rupture in dying cells. NINJ1 clusters into structurally diverse assemblies in dying cell membranes, particularly large filamentous assemblies with branched morphology. Molecular dynamics simulations show that NINJ1 can stably cap membrane edges. Therefore, NINJ1 is an interactive component of the eukaryotic cell membrane that functions as an in-built breaking point in response to activation of cell death.
Editorial Material
Multidisciplinary Sciences
Camilo Perez
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Multidisciplinary Sciences
Maximilian Huber, Javier Casares-Arias, Reinhard Faessler, Daniel J. Mueller, Nico Strohmeyer
Summary: Adopting a round cell morphology before mitosis is crucial. The authors found that, unlike interphase cells, mitotic cells cannot reinforce cell-ECM adhesion by engaging newly bound integrins via talin or vinculin. However, beta 1 integrins strengthen cell-cell adhesion in mitosis. This dual role of integrins weakens cell-ECM adhesion and strengthens cell-cell adhesion to prevent cell delamination during rounding and dividing.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Anna Stier, Samuel Gilberto, Weaam Mohamed, Lars N. Royall, Jonne Helenius, Ivan Mikicic, Tatjana Sajic, Petra Beli, Daniel J. Mueller, Sebastian Jessberger, Matthias Peter
Summary: CUL4A and CUL4B are paralogs that assemble cullin-RING E3 ubiquitin ligase (CRL) complexes regulating chromatin-associated cellular functions. The unique N-terminal extension of CUL4B is heavily phosphorylated during mitosis, and this phosphorylation is disrupted in the CUL4B-P50L mutation causing X-linked intellectual disability (XLID). Phenotypic characterization and mutational analysis showed that CUL4B phosphorylation is essential for mitosis progression and controls spindle positioning and cortical tension.
Article
Chemistry, Multidisciplinary
Seungkuk Ahn, Upnishad Sharma, Krishna Chaitanya Kasuba, Nico Strohmeyer, Daniel J. J. Muller
Summary: This study introduces a simple and scalable method to engineer biomimetic 3D fibrillar fibronectin matrices and characterizes how fibroblasts sense and adhere to these matrices. The findings demonstrate that fibroblasts accelerate adhesion initiation and strengthening to fibrillar fibronectin matrices compared to globular fibronectin. This immediate sensing and adhesion to fibrillar fibronectin also guide migration speed, persistency, and proliferation range in fibroblasts.