Article
Biochemistry & Molecular Biology
Jinbae Yu, Youngsik Woo, Heesun Kim, Sihyeon An, Sang Ki Park, Sung Key Jang
Summary: FMRP is a multifunctional protein encoded by the FMR1 gene, and its deficiency is associated with fragile X syndrome. We discovered that FMRP controls neuronal development by enhancing the translation of 4EBP2, a key downstream regulator. This finding contributes to our understanding of the pathology of fragile X syndrome.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Ludovica Congiu, Viviana Granato, Gabriele Loers, Ralf Kleene, Melitta Schachner
Summary: Adhesion molecule L1-70 plays an important role in the development of the nervous system, especially in mitochondrial function and processes such as neuronal survival and synapse formation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Gabriele Loers, Ralf Kleene, Viviana Granato, Ute Bork, Melitta Schachner
Summary: The neural cell adhesion molecule L1 plays important roles in cell migration, survival, differentiation, myelination, neurite outgrowth, and signaling in nervous system development and in adults. The proteolytic cleavage of L1 generates soluble and transmembrane fragments that regulate cellular functions. The study identifies the interaction between the extracellular LIR motif of L1 and LC3, which is important for neuronal survival and neurite outgrowth.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Ralf Kleene, Gabriele Loers, Melitta Schachner
Summary: Abnormal functions of L1 are associated with neural diseases. L1-55 fragment interacts with MeCP2, HP1, HistH1e, and other proteins via the KDET motif. These interactions regulate neurite outgrowth and neuronal survival, indicating the importance of L1 in neural system functions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Yuhei Tanaka, Haruki Watanabe, Kenji Shimoda, Kazufumi Sakamoto, Yoshitsune Hondo, Mitsuru Sentoku, Rikuto Sekine, Takahito Kikuchi, Kenji Yasuda
Summary: Conventional techniques cannot control the arrangement of axons and dendrites, but a new microfabrication technique has been developed to guide neurites during elongation, providing potential for fully direction-controlled on-chip neuronal network studies.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Multidisciplinary
Stefania Blasa, Mykola Borzenkov, Valentina Pastori, Lavinia Doveri, Piersandro Pallavicini, Giuseppe Chirico, Marzia Lecchi, Maddalena Collini
Summary: Targeted thermal stimulation induces morphological and functional differentiation of neurons, suggesting potential future application for modifying neuronal behavior in vivo.
Article
Pharmacology & Pharmacy
Dusadee Ospondpant, Xiong Gao, Tina Tingxia Dong, Karl Wah Keung Tsim
Summary: This study found that the extracts of Dracaena cochinchinensis stemwood can block the formation of A beta fibrils, prevent A beta-induced cell toxicity, and promote neuronal differentiation. These results suggest that the extracts of Dracaena cochinchinensis stemwood may have potential for Alzheimer's disease treatment.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Neurosciences
Chien-Sung Chiang, Ssu-Ju Fu, Chia-Lang Hsu, Chung-Jiuan Jeng, Chih-Yung Tang, Yi-Shuian Huang, Sung-Chun Tang
Summary: The study revealed significant differences in exosomal miRNA expression levels released by neurons under simulated ischemia/reperfusion injury, potentially involving multiple pathways related to cell survival, death processes, and neuronal signaling. Moreover, exosomes from oxygen and glucose-deprived conditions significantly impacted neuronal cell viability and neurite outgrowth, compared to exosomes from normoxic conditions.
NEUROMOLECULAR MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Vini Nagaraj, Roy Kim, Talia Martianou, Shyam Kurian, Ashana Nayak, Mukti Patel, Melitta Schachner, Thomas Theis
Summary: The L1 cell adhesion molecule is involved in neural development and repair by stimulating beneficial cellular functions and improving conditions in neurotrauma and the L1 syndrome. L1 agonistic mimetics trigger L1-dependent cellular signaling and promote neurite outgrowth and neuronal migration.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Batoul Chouaib, Pierre-Yves Collart-Dutilleul, Nicolas Blanc-Sylvestre, Richard Younes, Csilla Gergely, Cedric Raoul, Frederique Scamps, Frederic Cuisinier, Olivier Romieu
Summary: Stem cell-derived conditioned media show promise as pharmaceutical products for tissue regeneration, with human dental pulp cells (DPCs) conditioned medium enhancing neurite outgrowth in sensory neurons. Frozen storage does not affect activity, and 48 hours of conditioning is optimal. The addition of B-27 supplement enhances the regenerative effect of DPC secretome.
NEUROCHEMISTRY INTERNATIONAL
(2021)
Article
Biochemistry & Molecular Biology
Xihui Chen, Fangfang Liu, Bowen Li, Yufeng Wang, Lijuan Yuan, Anan Yin, Qi Chen, Weihong Hu, Yan Yao, Mengjie Zhang, YuanMing Wu, Kun Chen
Summary: Neurodegenerative diseases involve a wide range of disorders characterized by neuronal degeneration. Mutations in the FARS2 gene have been linked to various neurological disorders, affecting aminoacylation activity and leading to mitochondrial dysfunction. Studies using genetic models show how Fars2 deficiency impairs mitochondrial function and neuronal development, contributing to neurologic manifestations seen in patients.
CELL AND BIOSCIENCE
(2022)
Article
Food Science & Technology
Alex Xiong Gao, Jian Xiao, Tracy Chen-Xi Xia, Tina Ting-Xia Dong, Karl Wah-Keung Tsim
Summary: The ethanolic extract of peanut shell, known as PSE, is enriched with flavonoids that have neurotrophic functions. PSE can promote cell differentiation and neurofilament synthesis by activating the ERK1/2 and Akt signaling pathways. Phytochemicals such as luteolin, eriodictyol, and 5,7-dihydroxychromone in PSE may contribute to its neurotrophic effects.
JOURNAL OF FUNCTIONAL FOODS
(2022)
Article
Toxicology
Suzuna Go, Hisaka Kurita, Manami Hatano, Kana Matsumoto, Hina Nogawa, Masatake Fujimura, Masatoshi Inden, Isao Hozumi
Summary: Exposure to MeHg leads to impaired neuronal development primarily through alterations in DNA methylation and histone modification, indicating the importance of epigenetic changes in the neurotoxic effects of MeHg.
ARCHIVES OF TOXICOLOGY
(2021)
Article
Geriatrics & Gerontology
Shaoxiong Zhang, Chatrawee Duangjan, Tewin Tencomnao, Liangyu Wu, Michael Wink, Jinke Lin
Summary: Oolong tea polyphenols have neuroprotective and neuritogenesis properties, offering potential therapeutic effects against neurodegenerative diseases and aging.
FRONTIERS IN AGING NEUROSCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Jorge A. Sierra-Fonseca, Manuel Miranda, Siddhartha Das, Sukla Roychowdhury
Summary: The b gamma subunit of heterotrimeric G proteins interacts with both microtubules and actin filaments, playing a key role in modulating the neuronal cytoskeleton and participating in various aspects of neuronal differentiation. The interaction of Gb gamma with actin is independent of microtubules, and contributes to axon and growth cone formation during neuronal differentiation.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Genetics & Heredity
Srijana Adhikari, Neelam Thakur, Ujjowala Shrestha, Mohan K. Shrestha, Murarai Manshrestha, Bijay Thapa, Manish Poudel, Ajaya Kunwar
BMC MEDICAL GENETICS
(2020)
Article
Chemistry, Inorganic & Nuclear
Sabrina Schwarzbich, Claudia Horstmann nee Gruschka, Jasmin Simon, Lena Siebe, Alexander Moreth, Christiane Wiegand, Antonina Lavrentieva, Thomas Scheper, Anja Stammler, Hartmut Boegge, Gabriele Fischer von Mollard, Thorsten Glaser
INORGANIC CHEMISTRY
(2020)
Article
Microbiology
Marc Christian Hess, Svenja Bloess, Joe Max Risse, Karl Friehs, Gabriele Fischer von Mollard
Article
Neurosciences
Godwin Sokpor, Joachim Rosenbusch, Ajaya J. Kunwar, Michael Rickmann, Tran Tuoc, Silvio O. Rizzoli, Victor Tarabykin, Gabriele Fischer von Mollard, Kerstin Krieglstein, Jochen F. Staiger
Summary: Vti1a/1b play key roles in regulating neuronal growth and are essential for proper cortical development. Deletion of Vti1a/1b results in cortical dysgenesis in mice, particularly affecting the organization of cortical layer 5 and leading to neuronal apoptosis.
Article
Microbiology
Marc Christian Hess, Marvin Grollius, Valentin Duhay, Simon Koopmeiners, Svenja Bloess, Gabriele Fischer von Mollard
Summary: The study demonstrated the heterologous expression of four fungal LAAOs in Pichia pastoris, showing that the enzymatic activity of some enzymes for substrate conversion is influenced by N-glycosylation, with one enzyme's activity depending on specific N-glycosylation modification.
Article
Chemistry, Physical
Nils Janson, Tobias Heinks, Tobias Beuel, Sarfaraz Alam, Matthias Hoehne, Uwe T. Bornscheuer, Gabriele Fischer von Mollard, Norbert Sewald
Summary: The aldehyde tag is used for selective labeling of proteins and preparing antibody-drug conjugates, or immobilizing enzymes or antibodies. A new solid support, modified agarose with an aryl substituted pyrazolone, allows site-selective and efficient immobilization of aldehyde-containing molecules under physiological pH without the need for additives or catalysts. Compared to reductive amination, the modified agarose achieves higher loadings and activities in various buffers at different concentrations and temperatures.
Article
Biochemistry & Molecular Biology
Tobias Heinks, Jannik Paulus, Simon Koopmeiners, Tobias Beuel, Norbert Sewald, Matthias Hoehne, Uwe T. Bornscheuer, Gabriele Fischer von Mollard
Summary: Chiral and enantiopure amines can be synthesized through a dual-enzyme recycling system, which reduces the amount of transaminase co-substrate and alpha-keto acids by utilizing heterologously produced fungal hcLAAO4 and catalase. This system enables the kinetic resolution of racemic amines.
Review
Cell Biology
Sudip Paul, Anjuman Nahar, Mrinalini Bhagawati, Ajaya Jang Kunwar
Summary: This review summarizes the latest advances in cerebral palsy, including prevalence, risk factors, classification, diagnosis methods, and treatment strategies. It also analyzes the technological advancements in prevention, diagnosis, and treatment.
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2022)
Article
Cell Biology
Amna Music, Blanca Tejeda-Gonzalez, Diogo M. Cunha, Gabriele Fischer von Mollard, Sara Hernandez-Perez, Pieta K. Mattila
Summary: This study investigated the localization and function of SNARE protein Vti1b in B lymphocytes. The results showed that Vti1b was localized at the Golgi complex, MTOC, and lysosomal vesicles, and it was partially localized to internalized antigen vesicles and immunological synapse upon BCR activation. However, the absence of Vti1b in B cells did not affect BCR signaling, immunological synapse formation, or processing and presentation of internalized antigen.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Developmental Biology
Christian Bollmann, Susanne Schoening, Katharina Kotschnew, Julia Grosse, Nicole Heitzig, Gabriele Fischer von Mollard
Summary: This study reveals the importance of vti1a and vti1b in neuronal growth and confirms their interaction with other SNARE proteins. Loss of vti1a and vti1b leads to neuronal developmental defects and neurodegeneration.
NEURAL DEVELOPMENT
(2022)
Article
Chemistry, Physical
Tobias Heinks, Nicolai Montua, Michelle Teune, Jan Liedtke, Matthias Hoehne, Uwe T. Bornscheuer, Gabriele Fischer von Mollard
Summary: This study aims to compare the stability of soluble and immobilized transaminases from different species. Immobilization of transaminases enabled their reusability for at least 10 cycles and maintained full or high activity. Upscaled kinetic resolutions demonstrated high conversion, maintained enantioselectivity, and high product yields, showing their applicability.
Article
Chemistry, Physical
Tobias Heinks, Luisa M. Merz, Jan Liedtke, Matthias Hoehne, Luuk M. van Langen, Uwe T. Bornscheuer, Gabriele Fischer von Mollard, Per Berglund
Summary: In this study, four different amine transaminases (ATAs) were identified to catalyze the reductive amination of 5-(hydroxymethyl)furfural (HMF) and 2,5-diformylfuran (DFF). These ATAs were successfully immobilized on glutaraldehyde-functionalized amine beads and on amine beads by site-selective binding. Immobilized ATAs showed efficient reuse in the reductive amination of HMF with alanine as co-substrate, and the ATA-Spo exhibited high stability for reuse when isopropylamine was used as an amine donor. Additionally, immobilized ATA-Spo achieved high conversion in batch syntheses and continuous-flow catalysis using alanine or isopropylamine as amine donors.
Article
Biochemistry & Molecular Biology
Tobias Heinks, Simon Koopmeiners, Nicolai Montua, Norbert Sewald, Matthias Hoehne, Uwe T. Bornscheuer, Gabriele Fischer von Mollard
Summary: An enzyme cascade consisting of l-amino acid oxidase (hcLAAO4) and catalase (hCAT) was immobilized together with the (S)-selective amine transaminase (ATA-Vfl) to achieve higher reaction rates and more efficient H2O2-removal. The co-immobilized enzyme cascade showed good stability and high enantiomeric purity in preparative kinetic resolutions. However, further recycling was limited due to the instability of ATA-Vfl. The use of an engineered ATA-Vfl-8M in the immobilized enzyme cascade allowed a significant reduction in the amount of co-substrate required.
Article
Cell Biology
Lara Petersen, Rimma Bachmann, Sven Meinerz, Anne Tanz, Gabriele Fischer von Mollard
Summary: The epsin-related adaptor proteins Ent3p and Ent5p are involved in the formation of clathrin-coated vesicles during transport between trans-Golgi network and endosomes in yeast. They play different roles in transport, with Ent3p involved in the accumulation of Can1p-GFP in endosomes and Ent5p facilitating faster transport of Can1p-GFP to the vacuole for degradation. The C-terminal domain of Ent5p is sufficient to restore recycling of the secretory SNARE GFP-Snc1p between plasma membrane and TGN in cells with increased Ent3p and Ent5p levels. The interaction between Ent5p's ENTH domain and the SNARE Tlg2p is responsible for transport from early endosomes to the trans-Golgi network. The importance of Ent3p and Ent5p as cargo adaptors for distinct SNAREs is demonstrated.
