Article
Multidisciplinary Sciences
Shaheen A. Farhadi, Renjie Liu, Matthew W. Becker, Edward A. Phelps, Gregory A. Hudalla
Summary: Galectin-3 exhibits dynamic oligomerization and promiscuous binding, affecting cell behavior by influencing signaling pathways. Synthetic Gal3 constructs with defined valency can physically regulate extracellular signaling activity and cell death induction. Changes in multivalency impact caspase activation, membrane glycoprotein engagement, and intracellular pathway activation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Oncology
Valen Zhuoyou Yu, Shan Shan So, Maria Li Lung
Summary: This study investigated the oncogenic mechanism of p53 gene mutations in esophageal squamous cell carcinoma patients, revealing a novel GoF mechanism through which specific p53 mutants exhibit oncogenic features and contribute to ESCC tumorigenesis.
TRANSLATIONAL ONCOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yunier Serrano-Rivero, Julieta Salazar-Uribe, Marcela Rubio-Carrasquilla, Frank Camacho-Casanova, Oliberto Sanchez-Ramos, Alain Gonzalez-Pose, Ernesto Moreno
Summary: This study successfully generated anti-EGF nanobodies from a synthetic library, using a selection strategy involving four different elution steps and three rounds of selection. The results demonstrate the feasibility of selecting nanobodies against small antigens like EGF from synthetic libraries.
Article
Cell Biology
Aleksandra Gedaj, Dominika Zukowska, Natalia Porebska, Marta Pozniak, Mateusz Krzyscik, Aleksandra Czyrek, Daniel Krowarsch, Malgorzata Zakrzewska, Jacek Otlewski, Lukasz Opalinski
Summary: Fibroblast growth factors (FGFs) and their receptors (FGFRs) play crucial roles in development and homeostasis. This study reveals the binding of specific lectins, galectins, to the N-glycans of FGFs. These galectins modulate FGF4 signaling and cellular processes by adjusting its activity.
CELL COMMUNICATION AND SIGNALING
(2023)
Article
Multidisciplinary Sciences
Makoto Nakakido, Naoki Tanaka, Ayako Shimojo, Nobuhiro Miyamae, Kouhei Tsumoto
Summary: Respiratory infectious diseases pose a serious global threat, and novel antiviral materials are in high demand. In this study, a new platform was constructed to evaluate antiviral activity of surface coating materials using the M13 bacteriophage and phagemid system. The experimental system developed in this study provides rapid and effective screening, which can be applied to the development of novel antiviral materials.
Article
Biology
Ting Gong, Xuedi Zhang, Zhiyong Peng, Yinfeng Ye, Ruimeng Liu, Yinggui Yang, Zhugui Chen, Zhihao Zhang, Hongfei Hu, Shuang Yin, Yi Xu, Jing Tang, Youtan Liu
Summary: The levels of exosomal APN/CD13 in circulating plasma were found to be higher in patients and mice with sepsis-induced acute lung injury (ALI). Increased levels of exosomal APN/CD13 were associated with the severity of ALI and fatality in sepsis patients. Macrophage-secreted exosomal APN/CD13 regulated necroptosis of lung epithelial cells by binding to the cell surface receptor TLR4 to induce ROS generation, mitochondrial dysfunction, and NF-kappa B activation.
COMMUNICATIONS BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Mariam Ahmed Galal, Samhar Samer Alouch, Buthainah Saad Alsultan, Huda Dahman, Nouf Abdullah Alyabis, Sarah Ammar Alammar, Ahmad Aljada
Summary: This comprehensive review thoroughly explores the intricate involvement of insulin receptor isoforms and insulin-like growth factor receptors in the insulin and insulin-like growth factor signaling pathway, emphasizing their crucial roles in cancer development and resistance to anti-cancer drugs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Cardiac & Cardiovascular Systems
Nikolaos G. Frangogiannis
Summary: Myocardial fibrosis, characterized by the expansion of cardiac interstitium through deposition of extracellular matrix proteins, is a common pathophysiologic feature in various myocardial conditions. Activated fibroblasts and myofibroblasts play a central role in cardiac fibrosis, producing matrix proteins and triggering fibrogenic signalling cascades in response to stress. Immune cells, vascular cells, and cardiomyocytes can also contribute to fibrosis, while fibrotic changes may disrupt cardiac function and play a role in arrhythmogenesis.
CARDIOVASCULAR RESEARCH
(2021)
Article
Biology
Dina Aggad, Nicolas Brouilly, Shizue Omi, Clara Luise Essmann, Benoit Dehapiot, Cathy Savage-Dunn, Fabrice Richard, Chantal Cazevieille, Kristin A. Politi, David H. Hall, Remy Pujol, Nathalie Pujol, Piali Sengupta
Summary: In mutants lacking furrows, the normal connection between the epidermis and the cuticle is lost, and stacked structures are formed in the lateral epidermis. These structures connect the lateral epidermis to the cuticle and play a role in the stress response. Additionally, furrow mutants exhibit significant changes in the biomechanical properties of their skin.
Review
Biology
Yong Jin, Lele Ma, Wanying Zhang, Wen Yang, Qiyu Feng, Hongyang Wang
Summary: Extracellular vesicles (EVs) are naturally released membrane vesicles that act as carriers of proteins and RNAs for intercellular communication. With high efficiency and specificity, EVs provide a novel form of information transfer compared to classical signal transduction. In addition, EVs can collect extracellular information, extending the concept of signal transduction. The functions of EVs have been extensively studied and show great potential for clinical applications. However, the biogenesis of EVs and the regulation of this process by extracellular signals remain unclear.
BIOLOGICAL RESEARCH
(2022)
Review
Engineering, Biomedical
Robert C. H. Gresham, Chelsea S. Bahney, J. Kent Leach
Summary: Therapeutic approaches for musculoskeletal tissue regeneration commonly use growth factors to influence nearby cells for migration, proliferation, or differentiation. However, challenges in clinical translation include poor control and high dosages leading to side effects. The extracellular matrix plays a key role in regulating growth factor presentation and retention, impacting tissue regeneration.
BIOACTIVE MATERIALS
(2021)
Article
Chemistry, Multidisciplinary
Laura Franz, Jesko Koehnke
Summary: This study demonstrates a Sortase A-based approach for modifying peptides with enzymes from unrelated pathways, leading to the synthesis of a new-to-nature, hybrid ribosomal natural product in a one-pot reaction.
CHEMICAL COMMUNICATIONS
(2021)
Article
Cell Biology
Jakob Mitgau, Julius Franke, Camilla Schinner, Gabriele Stephan, Sandra Berndt, Dimitris G. Placantonakis, Hermann Kalwa, Volker Spindler, Caroline Wilde, Ines Liebscher
Summary: This study provides information on the structural requirements and forces needed for ECM-mediated activation of the GPR126 receptor. The N terminus of GPR126 acts as an allosteric module that can fine-tune receptor activation in a context-specific manner.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Dentistry, Oral Surgery & Medicine
M. P. Dieterle, A. Husari, T. Steinberg, X. Wang, I. Ramminger, P. Tomakidi
Summary: Novel findings in mechanotransduction have revealed the crucial role of YAP1 in periodontal disease development, cell behavior regulation, and its impact on oral squamous cell carcinoma and periodontitis.
JOURNAL OF DENTAL RESEARCH
(2021)
Article
Chemistry, Analytical
Ge Wang, Pengxue Yin, Jin Wang, Pengxin Ma, Yanbo Wang, Yuanyuan Cai, Huan Qi, Aihua Liu
Summary: A novel VEGF165-specific peptide was developed and utilized as a capture agent for an enzyme-linked chemiluminescence immunoassay (ELCLIA), demonstrating wide linear range and low detection limit. The ELCLIA method showed accurate and reproducible detection of VEGF165 in human serum samples, making it a promising biointerface for sensing and tumor metastasis related diagnosis and therapy.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Editorial Material
Biochemistry & Molecular Biology
Oscar Millet, Aitziber L. Cortajarena, Xavier Salvatella, Laura L. Kiessling, Jesus Jimenez-Barbero
ACS CHEMICAL BIOLOGY
(2020)
Article
Chemistry, Multidisciplinary
Mohammad Murshid Alam, Cassie M. Jarvis, Robert Hincapie, Craig S. McKay, Jiri Schimer, Carlos A. Sanhueza, Ke Xu, Roger C. Diehl, M. G. Finn, Laura L. Kiessling
Summary: DC surface lectins recognize glycans not typically present on host tissue to facilitate antigen uptake and presentation. Signal transduction through DC-SIGN depends on the ligand displayed on the VLP, promoting DC maturation and induction of a T(H)1-type immune response.
Editorial Material
Biochemistry & Molecular Biology
Laura L. Kiessling
ACS CHEMICAL BIOLOGY
(2021)
Review
Neurosciences
Joseph R. Klim, Greta Pintacuda, Leslie A. Nash, Irune Guerra San Juan, Kevin Eggan
Summary: TDP-43 is a multifunctional nucleic acid-binding protein associated with several devastating nervous system disorders. Dysfunction of TDP-43 leads to RNA homeostasis disruption and neural degeneration. Recent findings connecting TDP-43 dysfunction with improper splicing of STMN2 highlight a potential mechanistic link between TDP-43 proteinopathies and neuropathy.
TRENDS IN NEUROSCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Jordan S. Ho, Amira Gharbi, Baptiste Schindler, Oznur Yeni, Richard Bredy, Laurent Legentil, Vincent Ferrieres, Laura L. Kiessling, Isabelle Compagnon
Summary: Sequencing glycans is challenging due to their structural diversity. In this study, researchers used infrared multiple photon dissociation spectroscopy with mass spectrometry to differentiate between pyranose- and furanose-linked galactose residues, offering a solution to distinguish between these isomers. By analyzing hexose ammonium adducts, the researchers determined diagnostic MS-IR signatures of galactose anomers in different forms, demonstrating the utility of MS-IR for analyzing unique substructures in bacterial glycans.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Cell Biology
Xuan Huang, Kasper C. D. Roet, Liying Zhang, Amy Brault, Allison P. Berg, Anne B. Jefferson, Jackie Klug-McLeod, Karen L. Leach, Fabien Vincent, Hongying Yang, Anthony J. Coyle, Lyn H. Jones, Devlin Frost, Ole Wiskow, Kuchuan Chen, Rie Maeda, Alyssa Grantham, Mary K. Dornon, Joseph R. Klim, Marco T. Siekmann, Dongyi Zhao, Seungkyu Lee, Kevin Eggan, Clifford J. Woolf
Summary: Screening using patient-derived motor neurons has identified multiple effective targets for managing hyperexcitability in ALS, demonstrating the potential of human disease cell-based phenotypic screens in identifying clinically relevant targets for neurological disorders.
Review
Biochemistry & Molecular Biology
Laura L. Kiessling, Roger C. Diehl
Summary: The text discusses how proteins recognize sugars through CH-π interactions, highlighting the importance of electron-rich aromatic residues in glycan binding.
ACS CHEMICAL BIOLOGY
(2021)
Editorial Material
Biochemistry & Molecular Biology
Mia L. Huang, Laura L. Kiessling
ACS CHEMICAL BIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Victoria M. Marando, Daria E. Kim, Phillip J. Calabretta, Matthew B. Kraft, Bryan D. Bryson, Laura L. Kiessling
Summary: Researchers proposed a new strategy for chemoselective glycan modification utilizing cellular glycosyltransferases. They successfully synthesized and tested probes of D-arabinofuranose (D-Araf), which can selectively label arabinofuranose-containing glycans for the first time.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Editorial Material
Biochemical Research Methods
Amanda E. Dugan, Amanda L. Peiffer, Laura L. Kiessling
Summary: Interactions between carbohydrates and lectins play a key role in viral infection. Studying glycosylation has been challenging, but recent advancements in methods have allowed for atomic-level information about glycans on viruses and host cells.
Article
Neurosciences
Irune Guerra San Juan, Leslie A. Nash, Kevin S. Smith, Marcel F. Leyton-Jaimes, Menglu Qian, Joseph R. Klim, Francesco Limone, Alexander B. Dorr, Alexander Couto, Greta Pintacuda, Brian J. Joseph, D. Eric Whisenant, Caroline Noble, Veronika Melnik, Deirdre Potter, Amie Holmes, Aaron Burberry, Matthijs Verhage, Kevin Eggan
Summary: This study found that disrupting a single TDP43-regulated RNA gene is sufficient to cause substantial motor dysfunction, indicating that disruption of TDP43 function likely contributes to ALS.
Article
Biochemical Research Methods
Elad Deiss-Yehiely, Spencer D. Brucks, Natalie Boehnke, Andrew J. Pickering, Laura L. Kiessling, Paula T. Hammond
Summary: Targeted delivery of nanoparticle drug carriers is crucial for the development of nanomedicine. In this study, the authors investigated the association between modified nanoparticles and ovarian cancer cells using the well-characterized hyaluronic acid-CD44 binding pair. The results demonstrated that functionalizing nanoparticles with high molecular weight hyaluronic acid using the layer-by-layer adsorption method improved cell association.
BIOCONJUGATE CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Azin Saebi, Joseph S. Brown, Victoria M. Marando, Nina Hartrampf, Nicole M. Chumbler, Stephanie Hanna, Mackenzie Poskus, Andrei Loas, Laura L. Kiessling, Deborah T. Hung, Bradley L. Pentelute
Summary: The impermeable outer membrane of Pseudomonas aeruginosa can be bypassed by antibacterial proteins called S-type pyocins. Pyocins are investigated as a potential new class of antimicrobials against Pseudomonas infections due to their properties. In this study, a rapid production method for a pyocin S2 import domain was employed using automated fast-flow peptide synthesis, resulting in milligram quantities of the desired product. These synthetic pyocin domains can be used to understand the import mechanisms of P. aeruginosa and to develop selective antimicrobial agents that can bypass the outer membrane.
ACS CHEMICAL BIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Katherine I. Taylor, Jordan S. Ho, Hallie O. Trial, Alan W. Carter, Laura L. Kiessling
Summary: Probes that covalently label protein targets are crucial for identifying ligand-binding sites. Squaramides, as mild electrophiles, exhibit higher selectivity for binding site lysine residues, making them promising for affinity-based chemoproteomic probes.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Meeting Abstract
Immunology
Monica Kasturi Chanda, Claire E. Shudde, Taylor L. Piper, Yating Zheng, Adam H. Courtney
JOURNAL OF IMMUNOLOGY
(2022)