Article
Cell Biology
Dehao Huang, Qianhao Zhao, Mengyun Zhang, Qitong Weng, Qi Zhang, Kaitao Wang, Fang Dong, Hui Cheng, Fangxiao Hu, Jinyong Wang
Summary: In this study, the researchers used a mouse model with conditional expression of Hoxb5 to investigate its effect on multipotent blood progenitor cells. They found that induced expression of Hoxb5 in these cells led to the generation of a new cell type that can repopulate long-term multilineage haematopoiesis. RNA-seq analysis showed that these cells exhibited features of DNA replication and cell division.
CELL PROLIFERATION
(2022)
Article
Multidisciplinary Sciences
Minhe Xiao, Peng Zhou, Ziling Wang, Hanxianzhi Xiao, Xiao Chen, Rong Jiang, Yaping Wang
Summary: This study revealed that hematopoietic stem cells exhibit signs of aging, including decreased function and abnormal differentiation, which can lead to leukemia. The number of bone marrow hematopoietic cells started to decrease significantly after 45 years of age and senescent BMHCs gradually increased with age. BMHCs from individuals over 45 years old showed reduced proliferative capacity and increased G1-phase cell cycle arrest. Additionally, antioxidant capacity decreased and oxidative damage products increased with age, along with changes in the expression of senescence-associated proteins and genes.
SCIENTIFIC REPORTS
(2022)
Article
Medicine, Research & Experimental
Yihong Guan, Metis Hasipek, Dongxu Jiang, Anand D. Tiwari, Dale R. Grabowski, Simona Pagliuca, Sunisa Kongkiatkamon, Bhumika Patel, Salendra Singh, Yvonne Parker, Thomas LaFramboise, Daniel Lindner, Mikkael A. Sekeres, Omar Y. Mian, Yogen Saunthararajah, Jaroslaw P. Maciejewski, Babal K. Jha
Summary: Eltrombopag binds to the TET2 catalytic domain and inhibits its dioxygenase activity, leading to the expansion of normal hematopoietic stem cells and the prevention of neoplastic evolution in TET2 mutant malignant myeloid cells. This finding provides a scientific rationale for the treatment of myeloid malignancies associated with TET2 mutations or TET deficiency.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
Article
Multidisciplinary Sciences
Cheng Jin, Zhipeng Wang, Pengyu Li, Jielin Tang, Tao Jiao, Yiran Li, Jinhuan Ou, Dingfeng Zou, Mengzhen Li, Xinyu Mang, Jun Liu, Yanni Ma, Xiaolong Wu, Jie Shi, Shitao Chen, Manman He, Yan Lu, Ning Zhang, Shiying Miao, Fei Sun, Linfang Wang, Kai Li, Jia Yu, Wei Song
Summary: This study investigates the intercellular interactions between spermatogonial stem cell (SSC) and testicular niche in both adult mice and humans, uncovering the crucial role of C-C motif chemokine ligand 24 and insulin-like growth factor binding protein 7 in maintaining SSC homeostasis. The clinical relevance of specific paracrine factors in human fertility is also established.
Article
Multidisciplinary Sciences
Jette Lengefeld, Chia-Wei Cheng, Pema Maretich, Marguerite Blair, Hannah Hagen, Melanie R. McReynolds, Emily Sullivan, Kyra Majors, Christina Roberts, Joon Ho Kang, Joachim D. Steiner, Teemu P. Miettinen, Scott R. Manalis, Adam Antebi, Sean J. Morrison, Jacqueline A. Lees, Laurie A. Boyer, Omer H. Yilmaz, Angelika Amon
Summary: The size of stem cells plays a crucial role in their function, with small size being important for maintaining stem cell potential. Enlargement of hematopoietic stem cells under conditions of decreased function is associated with reduced proliferation and altered metabolism. Preventing stem cell enlargement can help prevent the loss of stem cell potential in exhausting conditions.
Editorial Material
Biochemistry & Molecular Biology
Christopher G. Hubert, Shaun R. Stauffer, Justin D. Lathia
Summary: A new signaling network that connects epigenetic regulation to self-renewal and therapeutic resistance in cancer stem cells is revealed in this study.
Article
Cell & Tissue Engineering
Gangqi Wang, Bram Heijs, Sarantos Kostidis, Rosalie G. J. Rietjens, Marije Koning, Lushun Yuan, Gesa L. Tiemeier, Ahmed Mahfouz, Sebastien J. Dumas, Martin Giera, Jesper Kers, Susana M. Chuva de Sousa Lopes, Cathelijne W. van den Berg, Bernard M. van den Berg, Ton J. Rabelink
Summary: Accumulating evidence demonstrates the importance of metabolism in cell fate determination. This study uses a multi-omics platform to study cell-type-specific dynamics in metabolism during nephrogenesis and highlights the relevance of understanding metabolic trajectories for guiding stem cell differentiation.
Article
Cell & Tissue Engineering
Anna Altshuler, Aya Amitai-Lange, Noam Tarazi, Sunanda Dey, Lior Strinkovsky, Shira Hadad-Porat, Swarnabh Bhattacharya, Waseem Nasser, Jusuf Imeri, Gil Ben-David, Ghada Abboud-Jarrous, Beatrice Tiosano, Eran Berkowitz, Nathan Karin, Yonatan Savir, Ruby Shalom-Feuerstein
Summary: This study revealed the differences in LSC populations at different locations, maintaining corneal health in the outer and inner limbal regions. It also found that LSC populations are abundant, following stochastic rules and neutral drift dynamics.
Article
Biochemistry & Molecular Biology
Ada Nowosad, Arnaud Besson
Summary: Lysosomes, initially known for their degradative functions, have emerged as signaling hubs that regulate various cellular processes and play a role in stem cell fate regulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell & Tissue Engineering
Shuai Ma, Si Wang, Yanxia Ye, Jie Ren, Ruiqing Chen, Wei Li, Jiaming Li, Liyun Zhao, Qian Zhao, Guoqiang Sun, Ying Jing, Yuesheng Zuo, Muzhao Xiong, Yuanhan Yang, Qiaoran Wang, Jinghui Lei, Shuhui Sun, Xiao Long, Moshi Song, Shuyang Yu, Piu Chan, Jianwei Wang, Qi Zhou, Juan Carlos Izpisua Belmonte, Jing Qu, Weiqi Zhang, Guang-Hui Liu
Summary: This study provides a comprehensive framework to explore aging and rejuvenating trajectories at single-cell resolution, revealing cellular and molecular programs that instruct systemic revitalization by blood-borne factors. Specifically, it identified hematopoietic stem and progenitor cells (HSPCs) as one of the most responsive cell types to young blood exposure, initiating a continuum of cell state changes and restoring a youthful transcriptional regulatory program and cell-cell communications in HSPCs.
Article
Cell Biology
Feng Yue, Stephanie N. Oprescu, Jiamin Qiu, Lijie Gu, Lijia Zhang, Jingjuan Chen, Naagarajan Narayanan, Meng Deng, Shihuan Kuang
Summary: In skeletal muscle satellite cells, the dynamic distribution of lipid droplets determines cell fate, with LDLow cells outperforming LDHigh cells in regeneration and self-renewal. Interventions in LD biogenesis and catabolism disrupt cell fate balance and impair the regenerative capacity of SCs.
Article
Multidisciplinary Sciences
Iqra Batool, Naim Bajcinca
Summary: This paper explores the dynamic effects on tumor cell populations, focusing on the impact of mutations in stem, progenitor, and fully differentiated cells, as well as the nonlinear feedback regulation from mature cells into stem and progenitor cell populations. The model provides steady-state solutions analytically and numerical simulations demonstrate various expected behavioral patterns in homeostatic regulation and cancer evolution. The findings suggest that mutated cells can experience significant growth with even a single somatic mutation, but under regulation, reach a steady-state, leading to either a stable or reduced healthy cell population.
Article
Cell Biology
Meghali Aich, Asgar Hussain Ansari, Li Ding, Vytautas Iesmantavicius, Deepanjan Paul, Chunaram Choudhary, Souvik Maiti, Frank Buchholz, Debojyoti Chakraborty
Summary: This study reveals that the lncRNA-associated protein TOBF1 regulates the alternative splicing of transcripts necessary for maintaining stem cell identity in mouse ESCs. One of the affected genes is serine/arginine splicing factor 1 (SRSF1), whose splicing leads to global changes in splicing and expression of downstream genes involved in the maintenance of ESC pluripotency. The study shows that TOBF1 chromatin occupancy, pluripotency-associated OCT-SOX binding motifs, and transcripts undergoing differential expression and splicing converge in specific nuclear territories, collectively contributing to the maintenance of mouse ESC identity.
Article
Biochemistry & Molecular Biology
Dan Li, Jihong Yang, Vikas Malik, Yuting Huang, Xin Huang, Hongwei Zhou, Jianlong Wang
Summary: RNA helicase eIF4A3 plays a crucial role in maintaining pluripotency of stem cells by post-transcriptionally controlling the cell cycle, particularly the efficient nuclear export of Ccnb1 mRNA.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Hematology
Fengjiao Wang, Jiahuan He, Siqi Liu, Ai Gao, Liu Yang, Guohuan Sun, Wanqiu Ding, Chuan-Yun Li, Fanglin Gou, Manman He, Fang Wang, Xiaoshuang Wang, Xiangnan Zhao, Ping Zhu, Sha Hao, Yanni Ma, Hui Cheng, Jia Yu, Tao Cheng
Summary: By analyzing 12 murine adult hematopoietic cell populations, this study identified 30796 editing sites and revealed the dynamic changes of the RNA editome during hematopoietic development. The highly edited Azin1 in HSPCs plays a crucial role in promoting cell differentiation.
Article
Multidisciplinary Sciences
Yumi Ueki, Matthew S. Wilken, Kristen E. Cox, Laura Chipman, Nikolas Jorstad, Kristen Sternhagen, Milesa Simic, Kristy Ullom, Masato Nakafuku, Thomas A. Reh
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2015)
Article
Developmental Biology
Shenyue Qin, Mayur Madhavan, Ronald R. Waclaw, Masato Nakafuku, Kenneth Campbell
Article
Developmental Biology
Jingyang Zhong, Hyoung-Tai Kim, Jungmook Lyu, Kazuaki Yoshikawa, Masato Nakafuku, Wange Lu
Article
Developmental Biology
Heather Chapman, Ronald R. Waclaw, Zhenglei Pei, Masato Nakafuku, Kenneth Campbell
Article
Cell Biology
Alejandro Lopez-Juarez, Jennifer Howard, Kristy Ullom, Lindsey Howard, Andrew Grande, Andrea Pardo, Ronald Waclaw, Yu-Yo Sun, Dianer Yang, Chia-Yi Kuan, Kenneth Campbell, Masato Nakafuku
GENES & DEVELOPMENT
(2013)
Correction
Cell Biology
Motoshi Nagao, Kenneth Campbell, Kevin Burns, Chia-Yi Kuan, Andreas Trumpp, Masato Nakafuku
JOURNAL OF CELL BIOLOGY
(2009)
Article
Endocrinology & Metabolism
Ahmed Shereen, Niza Nemkul, Dianer Yang, Faisal Adhami, R. Scott Dunn, Missy L. Hazen, Masato Nakafuku, Gang Ning, Diana M. Lindquist, Chia-Yi Kuan
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
(2011)
Article
Neurosciences
Nancy Lee, Myra K. Batt, Brigitte A. Cronier, Michele C. Jackson, Jennifer L. Bruno Garza, Dennis S. Trinh, Carter O. Mason, Rachel P. Spearry, Shayon Bhattacharya, Rachel Robitz, Masato Nakafuku, A. John MacLennan
JOURNAL OF NEUROSCIENCE
(2013)
Article
Neurosciences
Hiroko Nakatani, Elodie Martin, Hessameh Hassani, Adrien Clavairoly, Cecile L. Maire, Arthur Viadieu, Christophe Kerninon, Aurelie Delmasure, Magali Frah, Melanie Weber, Masato Nakafuku, Bernard Zalc, Jean-Leon Thomas, Francois Guillemot, Brahim Nait-Oumesmar, Carlos Parras
JOURNAL OF NEUROSCIENCE
(2013)
Article
Neurosciences
Lisa A. Ehrman, Diana Nardini, Sarah Ehrman, Tilat A. Rizvi, James Gulick, Maike Krenz, Biplab Dasgupta, Jeffrey Robbins, Nancy Ratner, Masato Nakafuku, Ronald R. Waclaw
JOURNAL OF NEUROSCIENCE
(2014)
Article
Neurosciences
Jimena Andersen, Noelia Urban, Angeliki Achimastou, Ayako Ito, Milesa Simic, Kristy Ullom, Ben Martynoga, Melanie Lebel, Christian Goritz, Jonas Frisen, Masato Nakafuku, Francois Guillemot
Article
Multidisciplinary Sciences
Andrew Grande, Kyoko Sumiyoshi, Alejandro Lopez-Juarez, Jennifer Howard, Bhuvaneswari Sakthivel, Bruce Aronow, Kenneth Campbell, Masato Nakafuku
NATURE COMMUNICATIONS
(2013)
Review
Developmental Biology
Masato Nakafuku, Angela del Aguila
WILEY INTERDISCIPLINARY REVIEWS-DEVELOPMENTAL BIOLOGY
(2020)
Article
Cell Biology
Joseph Salomone, Shenyue Qin, Temesgen D. Fufa, Brittany Cain, Edward Farrow, Bin Guan, Robert B. Hufnagel, Masato Nakafuku, Hee-Woong Lim, Kenneth Campbell, Brian Gebelein
Summary: The study investigates how Gsx homeodomain proteins gain DNA binding specificity by forming cooperative homodimers on different DNA sites. It also shows that the ratio of homodimer to monomer binding and Gsx protein levels define whether a gene is up-regulated or down-regulated. These findings provide a new paradigm for understanding how cooperative homeodomain transcription factor binding can increase target specificity and alter regulatory outcomes.
GENES & DEVELOPMENT
(2021)