Article
Biochemistry & Molecular Biology
Fujian Qin, Jiankang Li, Tianxiao Mao, Shuo Feng, Jing Li, Maode Lai
Summary: 2-hydroxybutyric acid (2HB) is a crucial regulatory factor in various diseases. We found that 2HB-producing bacteria in the human gut are mainly taxonomically classified as Proteobacteria and Firmicutes. Most gut bacteria have the ability to produce 2HB through multiple pathways, and Fusobacterium nucleatum has the strongest capacity. However, the gut microbiota has a minor contribution to serum 2HB levels.
Article
Materials Science, Multidisciplinary
Ximing Chen, Yan Ma, Wenwei Li, Qingwen Gui, Xuhua Zhan, Yaqin Lan, Weisong Pan, Tiean Zhou, Zhaohong Su
Summary: This study presents a functional coating with cell-specific adhesion and antiprotein adhesion properties prepared by electrochemical copolymerization, showing significantly improved cell adsorption and antiprotein adhesion characteristics.
ACS APPLIED POLYMER MATERIALS
(2021)
Article
Cell Biology
Megan M. Nelson, J. Damon Hoff, Mya L. Zeese, Gabriel Corfas
Summary: PARP1 plays a novel role in brain development by regulating Cajal-Retzius (CR) cells, neuronal density, and cell adhesion.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Shalom Lerrer, Anna S. Tocheva, Shoiab Bukhari, Kieran Adam, Adam Mor
Summary: Although PD-1 signaling has an inhibitory effect, it can also activate additional functions in T cells; T cells that proliferate more following PD-1 ligation are associated with effector and central memory phenotypes; the presence of transcriptionally and functionally distinct T cell populations responsive to PD-1 ligation provides new insights into the biology of PD-1 and suggests improving clinical outcomes of PD-1 blockade with T cell subset-specific approaches.
Article
Biochemistry & Molecular Biology
Feifei Du, Avin Hawez, Zhiyi Ding, Yongzhi Wang, Carl-Fredrik Ronnow, Milladur Rahman, Henrik Thorlacius
Summary: This study investigates the role of a protein called Mid1 in abdominal sepsis. The results show that Mid1 expression is increased in endothelial cells in septic mice and can be further increased by TNF-alpha stimulation in vitro. Knockdown of Mid1 using siRNA reduces the upregulation of ICAM-1 and the adhesion of neutrophils to endothelial cells. In vivo, silencing Mid1 decreases leukocyte adhesion in septic lungs and also reduces the expression of ICAM-1. The study also suggests that Mid1 may target a protein called PP2Ac in endothelial cells. Targeting the Mid1-PP2Ac axis could be a potential strategy to reduce lung inflammation in abdominal sepsis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Endocrinology & Metabolism
Khalid Siddiqui, Teena P. P. George, Muhammad Mujammami, Arthur Isnani, Assim A. A. Alfadda
Summary: Chronic hyperglycemia in patients with type 2 diabetes mellitus induces pathogenic changes in the vascular endothelium, leading to the development of microvascular complications. The levels of cell adhesion molecules and selectins are significantly different in diabetic patients with and without microvascular complications, and the baseline level of VCAM-1 is significantly associated with the development of microvascular complications.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Multidisciplinary Sciences
Yuh-Ru Julie Lee, Soichiro Yamada, Su Hao Lo
Summary: Biomolecular condensates formed by liquid-liquid phase separation include GFP-tagged tensin-1 (TNS1) proteins. Live-cell imaging revealed that TNS1 condensates emerge from the disassembling ends of focal adhesions (FAs) and their presence is cell cycle dependent. TNS1 condensates dissolve prior to mitosis and reappear when postmitotic daughter cells form new FAs, containing selected FA proteins and signaling molecules.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biochemistry & Molecular Biology
Yunxia Ma, Miljana Nenkov, Desiree Charlotte Schroeder, Mohamed Abubrig, Nikolaus Gassler, Yuan Chen
Summary: FBLN2, a member of the fibulin family, acts as a tumor suppressor in non-small cell lung cancer by modulating multiple signaling pathways and genes related to cell adhesion and ECM remodeling, indicating its potential as a biomarker for NSCLC subclassification.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Meiling Wang, Jie Liu, Yizeng Tu, Zihan Zhao, Jingjing Qu, Ka Chen, Yonglong Chen, Ying Sun, Hui Zhao, Yi Deng, Chuanyue Wu
Summary: Our study reveals that the focal adhesion protein RSU1 plays a critical role in suppressing ERK signaling during cell-ECM detachment through its interaction with prohibitin 2 (PHB2) in lipid rafts. The binding between RSU1 and PHB2 at specific sites is essential for the activation of ERK signaling in response to ECM adhesion. When cells detach from ECM, RSU1 associates with lipid rafts and interacts with PHB2, ultimately leading to downregulation of ERK signaling.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Victor Pui-Yan Ma, Yuesong Hu, Anna Kellner, Joshua M. Brockman, Arventh Velusamy, Aaron T. Blanchard, Brian D. Evavold, Ronen Alon, Khalid Salaita
Summary: T cells defend against cancer and viral infections by binding to specific peptide antigens on target cells. It has been found that the forces transmitted through the LFA-1 /ICAM-1 complex can tune T cell signaling and enhance T cell activation and TCR discrimination.
Review
Cardiac & Cardiovascular Systems
Jessica R. Pickett, Yuao Wu, Lucia F. Zacchi, Hang T. Ta
Summary: Vascular cell adhesion molecule-1 (VCAM-1) plays a crucial role in atherosclerosis and has been targeted for anti-atherosclerotic drug development. Most current VCAM-1-inhibiting drug candidates indirectly down-regulate VCAM-1 expression by inhibiting upstream cytokines, but optimizing dosage to balance pathological activity and normal physiological function is challenging. Recently, specific strategies that selectively inhibit VCAM-1 function, such as peptide- and antibody-based therapeutics, have emerged. However, studies on these novel VCAM-1-directed therapies are limited by the complexity of atherosclerosis pathophysiology.
CARDIOVASCULAR RESEARCH
(2023)
Article
Genetics & Heredity
Joel S. Serre, Mark C. Slabodnick, Bob S. Goldstein, Jeff C. Hardin
Summary: A hallmark of gastrulation is the establishment of germ layers by internalization of cells initially on the exterior. In C. elegans the end of gastrulation is marked by the closure of the ventral cleft, a structure formed as cells internalize during gastrulation, and the subsequent rearrangement of adjacent neuroblasts that remain on the surface. We found that a nonsense allele of srgp-1/srGAP leads to 10-15% cleft closure failure.
Article
Cell Biology
Elizabeth Riffo, Mario Palma, Matias Hepp, Diego Benitez-Riquelme, Vicente A. Torres, Ariel F. Castro, Roxana Pincheira
Summary: SALL2/Sall2 is a transcription factor that plays a positive role in cell migration, and its deficiency can lead to decreased cell motility and altered focal adhesion dynamics. Sall2 promotes cell migration by regulating integrin beta 1 expression and FAK autophosphorylation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Hematology
Jennell White, Michael U. Callaghan, Xiufeng Gao, Ke Liu, Ahmar Zaidi, Michael Tarasev, Patrick C. Hines
Summary: This study evaluated the relationship between FA-WB-VCAM and SCD severity, finding a strong correlation. SCD patients with high frequency of self-reported VOCs exhibited a pro-adhesive phenotype at steady state, potentially impacting the frequency of VOC occurrences.
BRITISH JOURNAL OF HAEMATOLOGY
(2022)
Article
Immunology
Jae-Young Park, Hyo-Min Park, Seonhwa Kim, Kyeong-Bae Jeon, Chae-Min Lim, Jin Tae Hong, Do-Young Yoon
Summary: IL-32 theta A94V, a mutant isoform, can inhibit monocyte-endothelial adhesion by binding to integrins aVb3 and aVb6, thus reducing the early step of atherosclerosis.
FRONTIERS IN IMMUNOLOGY
(2023)
Correction
Biochemistry & Molecular Biology
Yejin Jeong, Jinuk Kim, Hee-Jung Choi, Ka Young Chung
BIOMOLECULES & THERAPEUTICS
(2022)
Article
Biochemistry & Molecular Biology
Elise S. Bruguera, Jacob P. Mahoney, William Weis
Summary: Many membrane proteins function as dimers or larger oligomers. However, the detergent solubilization used for biochemical and structural studies disrupts the functional oligomerization. In this study, a technique using split GFP tether is described to obtain purified homogeneous membrane protein dimers in nanodiscs. This method provides several advantages such as stability, easy screening and dimer-specific purification handle.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Correction
Multidisciplinary Sciences
Jinuk Kim, Wonhee Han, Taeyong Park, Eun Jin Kim, Injin Bang, Hyun Sik Lee, Yejin Jeong, Kyeonghwan Roh, Jeesoo Kim, Jong-Seo Kim, Chanhee Kang, Chaok Seok, Jin-Kwan Han, Hee-Jung Choi
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Chaehee Park, Jinuk Kim, Seung-Bum Ko, Yeol Kyo Choi, Hyeongseop Jeong, Hyeonuk Woo, Hyunook Kang, Injin Bang, Sang Ah Kim, Tae-Young Yoon, Chaok Seok, Wonpil Im, Hee-Jung Choi
Summary: The study of the interaction mechanism between NPY and its receptor Y1R provides important molecular insights into the role of NPY in physiological processes such as food intake, stress response, and anxiety.
NATURE COMMUNICATIONS
(2022)
Article
Engineering, Biomedical
Ju-Chan Park, Jumee Kim, Hyun-Ki Jang, Seung-Yeon Lee, Keun-Tae Kim, Eun-Ji Kwon, Seokwoo Park, Hyun Sik Lee, Hyewon Choi, Seung-Yeol Park, Hee-Jung Choi, Soon-Jung Park, Sung-Hwan Moon, Sangsu Bae, Hyuk-Jin Cha
Summary: This study successfully established multiple isogenic disease models of GNE myopathy by using base editors to construct hPSCs. Different mutations in different domains of the GNE gene were found to cause mutation-specific hyposialylation and gene expression changes, which were closely related to pathological clinical phenotypes. Furthermore, treatment with a drug candidate currently under clinical trials showed a mutation-specific drug response in GNE myopathy disease models.
Article
Anatomy & Morphology
Rachel Prins, Peter Windsor, Bill R. Miller, Stephanie Maiden
Summary: This study reveals a novel role of UNC-33/CRMP in epidermal development in C. elegans and investigates the effects of different mutations on UNC-33 using computational approaches.
DEVELOPMENTAL DYNAMICS
(2022)
Article
Biochemistry & Molecular Biology
Hyun-Kyu Choi, Hyunook Kang, Chanwoo Lee, Hyun Gyu Kim, Ben P. Phillips, Soohyung Park, Charlotte Tumescheit, Sang Ah Kim, Hansol Lee, Soung-Hun Roh, Heedeok Hong, Martin Steinegger, Wonpil Im, Elizabeth A. Miller, Hee-Jung Choi, Tae-Young Yoon
Summary: This study reveals the folding pathway of purified human glucose transporter 3 using single-molecule magnetic tweezers. The N-terminal major facilitator superfamily fold forms first and serves as a template for the C-terminal fold. The insertion of hydrophilic transmembrane helices is facilitated by the endoplasmic reticulum membrane protein complex, and final assembly depends on specific lipids.
NATURE CHEMICAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Leanna M. Owen, Nicolas A. Bax, William I. Weis, Alexander R. Dunn
Summary: This study found that talin ABS3 forms long-lived catch bonds with F-actin under force, which is necessary for normal focal adhesion formation. The results support a mechanism in which talin ABS3 preferentially binds to and orients actin filaments, leading to long-range order in the actin cytoskeleton. Talin ABS3 may serve as a molecular AND gate that allows focal adhesion growth only when sufficient integrin density, F-actin polarization, and mechanical tension are simultaneously present.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Correction
Multidisciplinary Sciences
Chaehee Park, Jinuk Kim, Seung-Bum Ko, Yeol Kyo Choi, Hyeongseop Jeong, Hyeonuk Woo, Hyunook Kang, Injin Bang, Sang Ah Kim, Tae-Young Yoon, Chaok Seok, Wonpil Im, Hee-Jung Choi
NATURE COMMUNICATIONS
(2022)
Article
Cell Biology
Soungyub Ahn, Heeseung Yang, Sangwon Son, Hyun Sik Lee, Dongjun Park, Hyunsoo Yim, Hee-Jung Choi, Peter Swoboda, Junho Lee
Summary: Cilia are crucial for environmental interaction and tissue function. This study uncovers the regulatory mechanism that confers a specific identity on IL2 neurons in C. elegans, revealing the conservation of genetic modules for distinct behaviors.
Article
Biology
Seung-Bum Ko, Emiko Mihara, Yedarm Park, Kyeonghwan Roh, Chanhee Kang, Junichi Takagi, Injin Bang, Hee-Jung Choi
Summary: The linker domain of Frizzled receptors has differential effects on canonical and non-canonical Wnt signaling, with FZD chimeras affecting Wnt protein binding and downstream signaling protein recruitment. These results suggest that the FZD linker plays an important role in specific Wnt binding and intracellular Wnt signaling.
COMMUNICATIONS BIOLOGY
(2022)
Article
Biology
Amy Wang, Alexander R. Dunn, William Weis
Summary: Mechanotransduction at cell-cell adhesions is crucial for the structural integrity, organization, and morphogenesis of epithelia. The E-cadherin/beta-catenin/alpha E-catenin complexes can sense and transmit mechanical load by interacting with F-actin.
Article
Biochemistry & Molecular Biology
Jacob P. Mahoney, Elise S. Bruguera, Mansi Vasishtha, Lauren B. Killingsworth, Saw Kyaw, William Weis
Summary: In the Wnt-beta-catenin pathway, Fzd-Dvl binding is enhanced by increased concentration of PI(4,5)P-2, generated by Dvl-associated lipid kinases in response to Wnt. However, binding to Fzd does not promote Dvl DEP domain dimerization.
Article
Biochemistry & Molecular Biology
Hyunook Kang, Chaehee Park, Yeol Kyo Choi, Jungnam Bae, Sohee Kwon, Jinuk Kim, Chulwon Choi, Chaok Seok, Wonpil Im, Hee-Jung Choi
Summary: Neuropeptide Y (NPY) and its receptors play a role in regulating appetite and emotion in the brain. The Y1R and Y2R receptors activate GI signaling in response to NPY stimulation, but they have different physiological responses to food intake. The structures of the PYY(3-36)-Y2R-Gi and NPY-Y2R-Gi complexes were determined using cryo-electron microscopy, revealing how PYY(3-36) specifically binds to Y2R. Furthermore, it was found that Y2R prefers G protein signaling over b-arrestin signaling, while Y1R does not show a preference between these two pathways.
Article
Biochemistry & Molecular Biology
Nicolas A. Bax, Amy Wang, Derek L. Huang, Sabine Pokutta, William I. Weis, Alexander R. Dunn
Summary: Classical cadherins are transmembrane proteins that connect neighboring cells. The cadherin-catenin complex transmits forces that drive tissue morphogenesis and wound healing. How multiple cadherin-complexes cooperate to reinforce cell-cell junctions in response to load remains poorly understood.
JOURNAL OF MOLECULAR BIOLOGY
(2023)
