Article
Multidisciplinary Sciences
Omar M. Khan, Jorge Almagro, Jessica K. Nelson, Stuart Horswell, Vesela Encheva, Kripa S. Keyan, Bruce E. Clurman, Ambrosius P. Snijders, Axel Behrens
Summary: The study demonstrates that TRIP12 mediates branched K11-linked ubiquitylation of FBW7, regulating its stability and the abundance of a subset of SCFFBW7 substrates, with concomitant FBW7 inactivation rescuing the effects of TRIP12 deficiency.
NATURE COMMUNICATIONS
(2021)
Editorial Material
Biochemistry & Molecular Biology
Laura A. Hehl, Brenda A. Schulman
Summary: Tsai et al.1 and Mark et al.2 show that the E3 ligase UBR5 mediates broad regulation by selectively targeting agonist-bound nuclear hormone receptors, MYC, and other transcriptional regulators not incorporated into active gene expression complexes.
Editorial Material
Biochemistry & Molecular Biology
Yuan Xie, Minglei Zhao
Summary: Proper regulation of protein degradation is crucial for cell physiology. In the recent issue of Cell, Baek et al. revealed the assembly and disassembly mechanism of a widely present ubiquitin ligase class called CRL, through a key regulatory protein, CAND1.
Editorial Material
Pharmacology & Pharmacy
Asad M. Taherbhoy, Danette L. Daniels
Summary: This article introduces targeted protein degradation (TPD) and proposes multiple strategies using UBR5 as an E3 ligase to drive degradation of key transcriptional targets implicated in disease.
TRENDS IN PHARMACOLOGICAL SCIENCES
(2023)
Article
Chemistry, Medicinal
Xuejiao Dong, Pengcheng Wang, Yinsheng Wang
Summary: This study developed a chemoproteomic strategy to identify proteins binding to trivalent arsenic. The results suggest that trivalent arsenic may perturb protein homeostasis by directly binding to molecular chaperones.
CHEMICAL RESEARCH IN TOXICOLOGY
(2022)
Article
Multidisciplinary Sciences
Christina Priest, Rohith T. Nagari, Lara Bideyan, Stephen D. Lee, Alexander Nguyen, Xu Xiao, Peter Tontonoz
Summary: The study illustrates the role of BRAP in modulating the hepatic Hippo pathway by regulating MST2, impacting liver cell morphology and turnover. Loss of BRAP leads to significant morphological changes in the liver, increased hepatocyte proliferation, cell death, and inflammation. The altered Hippo pathway signaling in Brap knockout mice affects liver lipid accumulation in dietary models of obesity, highlighting its relevance to human liver disease.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Medicine, Research & Experimental
Zongang Liu, Mengnan Zhao, Xizi Jiang, Yao Zhang, Suning Zhang, Yitong Xu, Hongjiu Ren, Hongbo Su, Huanxi Wang, Xueshan Qiu
Summary: This study elucidates the role of KLHL17 in the development and progression of NSCLC using clinical samples and NSCLC cell lines. The results show that upregulated KLHL17 in NSCLC promotes the proliferation and migration of tumor by activating Ras/MAPK signaling pathway, and suggest that KLHL17 may be a novel therapeutic target for the treatment of NSCLC.
LABORATORY INVESTIGATION
(2022)
Article
Chemistry, Medicinal
Fanye Meng, Chenxi Xu, Kwang-Su Park, H. Umit Kaniskan, Gang Greg Wang, Jian Jin
Summary: In this study, a first-in-class NSD2 proteolysis targeting chimera (PROTAC) degrader was discovered, which effectively degraded NSD2 and CRBN. This compound was more effective in suppressing cancer cell growth compared to existing inhibitors and showed bioavailability in mice.
JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Review
Cell Biology
Fumiyo Ikeda
Summary: Ubiquitin modifies diverse substrates through various conjugation types, regulating a wide range of biological functions. While the C-terminus of ubiquitin traditionally forms isopeptide or peptide bonds with protein substrates, recent studies have revealed that it can also form atypical oxyester bonds, targeting both proteinaceous and nonproteinaceous substrates such as sugars and lipids. The understanding of how nonprotein ubiquitination affects substrate and cellular functions is still incomplete. This review discusses recent discoveries in ubiquitination and its potential impacts on biology.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2023)
Article
Cell Biology
Allyson M. Cochran, Jacki Kornbluth
Summary: NK3.3-derived extracellular vesicles (EVs) exhibit anti-tumor activity by containing cytolytic molecules and various RNA species, inhibiting proliferation and inducing apoptosis in a tumor-specific manner. These EVs have the potential to be a safe and effective immunotherapeutic agent.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Oncology
Gatha Thacker, Mukul Mishra, Akshay Sharma, Anil Kumar Singh, Sabyasachi Sanyal, Arun Kumar Trivedi
Summary: The study demonstrates that CDK2 potentiates SKP2-mediated C/EBP alpha degradation in AML, leading to differentiation block. CDK2 promotes C/EBP alpha ubiquitination and reduces its protein levels, while simultaneous SKP2 depletion restores C/EBP alpha expression and function.
Article
Chemistry, Medicinal
Serah W. Kimani, Julie Owen, Stuart R. Green, Fengling Li, Yanjun Li, Aiping Dong, Peter J. Brown, Suzanne Ackloo, David Kuter, Cindy Yang, Miranda MacAskill, Stephen Scott MacKinnon, Cheryl H. Arrowsmith, Matthieu Schapira, Vijay Shahani, Levon Halabelian
Summary: DCAF1 serves as a subunit for RING-type CRL4(DCAF1) and HECT family EDVPDCAF1 E3 ubiquitin ligases in substrate recruitment. The WDR domain of DCAF1 acts as a binding platform for substrate proteins and is targeted by HIV and SIV lentiviral adaptors. This study used a proteome-scale drug-target interaction prediction model to identify ligands for the DCAF1 WDR domain through biophysical screening and X-ray crystallography, confirming the selective binding of a predicted ligand. The findings demonstrate the successful application of artificial intelligence-enabled virtual screening methods in the absence of previously known ligands.
JOURNAL OF CHEMICAL INFORMATION AND MODELING
(2023)
Review
Immunology
Haoran Cui, Yaxian Zhang, Leiliang Zhang
Summary: Poxviruses have evolved various mechanisms to evade innate immunity, some of which involve poxvirus-encoded E3 ubiquitin ligases and adaptor proteins. These proteins can be categorized into five groups based on their functional domains and ubiquitin transfer mechanisms. Most known substrates of poxvirus E3 ubiquitin ligases are components of the innate immune system. Current research progress provides mechanistic insights into the interaction between these viruses and their hosts.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Fisheries
Jian Zhang, Xiangyi Sun, Xuepeng Li, Shuning Zhang, Xiudan Xu, Jixing Feng
Summary: Many viruses hijack the host's ubiquitin-proteasome system (UPS) for successful infection. This study identified and characterized a protein called SmE2D2 in turbot, which interacts with the RBIV-C1 virus. The results show that SmE2D2 is an E2 ubiquitin-conjugating enzyme that is used by RBIV-C1 to promote efficient replication.
Article
Biochemistry & Molecular Biology
Kiran Challa, Christoph D. Schmid, Saho Kitagawa, Anais Cheblal, Vytautas Iesmantavicius, Andrew Seeber, Assaf Amitai, Jan Seebacher, Michael H. Hauer, Kenji Shimada, Susan M. Gasser
Summary: In eukaryotic cells, checkpoint activation leads to degradation of core histones, resulting in reduced nucleosome occupancy. Extensive changes in chromatin-associated protein composition were observed after DNA damage, including loss of core histones, recruitment of ubiquitin ligases, and compromised DNA strand invasion kinetics during repair. This study provides a comprehensive overview of the genome-wide chromatin response to DNA damage.
Article
Immunology
Dulce Maroni, Sandeep Rana, Chandrani Mukhopadhyay, Amarnath Natarajan, Mayumi Naramura
IMMUNOLOGY LETTERS
(2015)
Article
Multidisciplinary Sciences
Chandrani Mukhopadhyay, Aleata Triplett, Tom Bargar, Carol Heckman, Kay-Uwe Wagner, Mayumi Naramura
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2016)
Article
Biochemistry & Molecular Biology
Neha Nandwani, Parag Surana, Jayant B. Udgaonkar, Ranabir Das, Shachi Gosavi
Review
Biochemistry & Molecular Biology
Bhopal Mohapatra, Gulzar Ahmad, Scott Nadeau, Neha Zutshi, Wei An, Sarah Scheffe, Lin Dong, Dan Feng, Benjamin Goetz, Priyanka Arya, Tameka A. Bailey, Nicholas Palermo, Gloria E. O. Borgstahl, Amarnath Natarajan, Srikumar M. Raja, Mayumi Naramura, Vimla Band, Hamid Band
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2013)
Article
Developmental Biology
Gautam K. Malhotra, Xiangshan Zhao, Emily Edwards, Janel L. Kopp, Mayumi Naramura, Maike Sander, Hamid Band, Vimla Band
BMC DEVELOPMENTAL BIOLOGY
(2014)
Meeting Abstract
Oncology
Tameka A. Bailey, Haitao Luan, Amy L. Wells, David L. Kelly, Mayumi Naramura, Srikumar Raja, Vimla Band, Hamid Band
Meeting Abstract
Oncology
Shakur Mohibi, Channabasavaiah Gurumurthy, Sameer Mirza, Alo Nag, Bryan Katafiasz, Aditya Bele, Mayumi Naramura, Hamid Band, Vimla Band
Article
Biochemistry & Molecular Biology
Shakur Mohibi, Channabasavaiah Basavaraju Gurumurthy, Alo Nag, Jun Wang, Sameer Mirza, Yousaf Mian, Meghan Quinn, Bryan Katafiasz, James Eudy, Sanjit Pandey, Chittibabu Guda, Mayumi Naramura, Hamid Band, Vimla Band
JOURNAL OF BIOLOGICAL CHEMISTRY
(2012)
Article
Immunology
Joseph Y. Shin, Wenhuo Hu, Mayumi Naramura, Christopher Y. Park
JOURNAL OF EXPERIMENTAL MEDICINE
(2014)
Article
Multidisciplinary Sciences
Tomas Helikar, Naomi Kochi, Bryan Kowal, Manjari Dimri, Mayumi Naramura, Srikumar M. Raja, Vimla Band, Hamid Band, Jim A. Rogers
Article
Multidisciplinary Sciences
Chandrani Mukhopadhyay, Xiangshan Zhao, Dulce Maroni, Vimla Band, Mayumi Naramura
Article
Multidisciplinary Sciences
Neha Nandwani, Parag Surana, Hitendra Negi, Nahren M. Mascarenhas, Jayant B. Udgaonkar, Ranabir Das, Shachi Gosavi
NATURE COMMUNICATIONS
(2019)
Article
Chemistry, Multidisciplinary
Carmen Suay-Corredera, Maria Rosaria Pricolo, Diana Velazquez-Carreras, Divya Pathak, Neha Nandwani, Carolina Pimenta-Lopes, David Sanchez-Ortiz, Inigo Urrutia-Irazabal, Silvia Vilches, Fernando Dominguez, Giulia Frisso, Lorenzo Monserrat, Pablo Garcia-Pavia, David de Sancho, James A. Spudich, Kathleen M. Ruppel, Elias Herrero-Galan, Jorge Alegre-Cebollada
Summary: Hypertrophic cardiomyopathy (HCM) is a disease caused by mutations in sarcomeric proteins, with many mutations not affecting protein structure or stability. Research shows that these mutations may disrupt the nanomechanics of cMyBP-C, affecting its regulatory role in actomyosin filaments.
Meeting Abstract
Biophysics
Neha Nandwani, Darshan V. Trivedi, Saswata S. Sarkar, Makenna Morck, Kathleen Ruppel, James A. Spudich
BIOPHYSICAL JOURNAL
(2020)
Meeting Abstract
Biophysics
Debanjan Bhowmik, Neha Nandwani, Kathleen Ruppel, Chao Liu, James A. Spudich
BIOPHYSICAL JOURNAL
(2020)