期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 107, 期 15, 页码 6731-6736出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0909533107
关键词
membrane dynamics; microrheology; quantitative phase imaging
资金
- National Institutes of Health [P41-RR02594-18]
- Cambridge Foundation Fellowship
- Whitaker Health Sciences Fellowship
- Samsung Scholarship
- National Science Foundation [08-46660 CAREER, NSF-DMR-0907212]
- Direct For Mathematical & Physical Scien [0907212] Funding Source: National Science Foundation
- Division Of Materials Research [0907212] Funding Source: National Science Foundation
The human red blood cell (RBC) membrane, a fluid lipid bilayer tethered to an elastic 2D spectrin network, provides the principal control of the cell's morphology and mechanics. These properties, in turn, influence the ability of RBCs to transport oxygen in circulation. Current mechanical measurements of RBCs rely on external loads. Here we apply a noncontact optical interferometric technique to quantify the thermal fluctuations of RBC membranes with 3 nm accuracy over a broad range of spatial and temporal frequencies. Combining this technique with a new mathematical model describing RBC membrane undulations, we measure the mechanical changes of RBCs as they undergo a transition from the normal discoid shape to the abnormal echinocyte and spherical shapes. These measurements indicate that, coincident with this morphological transition, there is a significant increase in the membrane's shear, area, and bending moduli. This mechanical transition can alter cell circulation and impede oxygen delivery.
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