期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 107, 期 20, 页码 9141-9146出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1002538107
关键词
cell movement; force generation; retrograde flow; Arp2/3
资金
- Engineering and Physical Sciences Research Council
- University of Cambridge
- National Institutes of Health [R37 GM29994]
- MRC [MC_U105178939] Funding Source: UKRI
- Medical Research Council [MC_U105178939] Funding Source: researchfish
Many cells crawl by extending an actin-rich pseudopod. We have devised a simulation that describes how the polymerization kinetics of a branched actin filament network, coupled with excluded volume effects, powers the motility of crawling cells such as amoebae and fish keratocytes. Our stochastic simulation is based on the key fundamental properties of actin polymerization, namely growth, shrinkage, capping, branching, and nucleation, and also includes contributions from the creation and breaking of adhesive contacts with the substrate together with excluded volume effects related to filament packing. When reasonable values for appropriate constants were employed, this simulation generated a force-velocity relationship that resembled closely that observed experimentally. Our simulations indicated that excluded volume effects associated with actin filament branching lead to a decreased packing efficiency and resultant swelling of the cytoskeleton gel that contributes substantially to lamellipod protrusion.
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